Transfer of small chromosome fragments of Agropyron elongatum to wheat chromosome via asymmetric somatic hybridization

~~Transfer of small chromosome fragments of Agropyron elongatum to wheat chromosome via asymmetric somatic hybridization1 .Dong,Y.C,GenePools of common wheat,Journal of Triticeae CroPs(in Chinese),2000,20(3):78-81. 2 .Wei,Y.M.,Zheng,YL.Zhou,R.H., Detectlon of the rye chro- matin in multisPikelet wheat germplasm 10-A background using fluorescence in situ hybridization(FISH)and RFLP markers,Acta Bot.Sinica(in Chinese),1999,41(7):722-725. 3 .Xiang,E N.,Xia,G M.…     

Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells

~~Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells1. Jin, Q., Yuan, Z., Xu, J., Wang, Y., Shen, Y., Lu, W., Wang, J., Liu, H., Yang, J., Yang, P., Zhang, X., Zhang, J., Yang, G, Wu, H., Qu, D., Dong, J., Sun, L., Xue, Y, Zhao, A., Gao, Y., Zhu, J., Kan, B., Ding, K.. Chen, S., Cheng, H., Yao, Z., He, B., Chen, R., Ma, D., Qiang, B., Wen, Y, Hou, Y., Yu, J., Genome sequence of Shigella flexneri 2…     

Intersectional gene inactivation: there is more to conditional mutagenesis than Cre

正The efforts of many laboratories worldwide and large scale international mutagenesis consortia have resulted in mutagenesis of nearly all mouse genes, and present efforts are devoted to large scale phenotyping of germline null mutant mice (Ayadi et al., 2012). Although early lethality associated with loss of function of a given gene may prevent elucidating its function at a later stage or in a given tissue, conditional alleles in which a critical part of the gene is flanked by recombinase target sites provide an opportunity for addressing gene function at later stages (Branda and Dymecki,2004). Fortunately, many of the mutant alleles generated by the consortia allow for conditional mutagenesis using site directed recombinases.     

Narrow distribution of cyanophage psbA genes observed in two paddy waters of Northeast China by an incubation experiment

正Dear Editor,Cyanophages are viruses that infect cyanobacteria.They play an important role in shaping the genetic and functional diversity of themselves and their hosts through genetic exchange and shuffling(Lindell et al.,2005;Singh et al.,2012).The psb A gene of cyanophages is a"hostderived"gene,and its encoded D1 protein is capable of     

蓝胸秧鸡亚种新纪录

蓝胸秧鸡 Rallus striatus albiventer (Swainson) Rallus albiventer Swainson, 1838, Animals in Menageries: 337 (印度)。Aii, S., and S.D. Ripley 1980 Handbook of the birds of Indai and Pakistan. Vol. 2. P: 153—154.; Deignan, H.G. 1945 The birds of northern Thailand. P: 103—104.; Smythies, B.E. 1953 The birds of Burina. P: 452—454. and P; 609.     

Identification and expression profiling of putative odorant-binding proteins in the malaria mosquitoes, Anopheles gambiae and A.arabiensis

~~Identification and expression profiling of putative odorant-binding proteins in the malaria mosquitoes, Anopheles gambiae and A. arabiensis1. Curtis, C. F., Introduction 1: An overview of mosquito biology, behaviour and importance, in Olfaction in Mosquito-Host Interactions (eds. Bock, G. R.. Cardew, G.), New York: Wiley, 1996, 3-7. 2. Nighom, A., Hildebrand. J. G.. Dissecting the molecular mechanisms of olfaction in a malaria-vector mosquito, PNAS, 2002, 99(3): 1113-…     

JURASSIC-CRETACEOUS BOUNDARY OF DONG''ANZHEN, RAOHE, HEILONGJIANG WITH A NEW KNOWLEDGE OF BUCHIA

1. FOREWORD Jurassic and Cretaceous: deposits are widely dispersed over Northeast China. However, they are mostly continental strata. In 1958, marine beds with ammonites were found for the first time by Xu, Y. Q. and Zhao, D. Z. of the Geological Bureau of Heilongjiang from Longzhaogou, Hulin County, Heilongjiang. The ammonites were identified by Wang, Y. G. (1983., p.100) as Arctocephalites (Crarocephalites) hulinensis Wang, Stenocadoceras? sp., etc., with their age tecognized as the Bathonian, In the 1970's, blackish deposits with the fossil fishes Sungarichthys longicephalus Takai, etc. were found from western Jilin and studied by Zhang, M. M. et al. (1977) who considered them as belonging to the early-middle     

Mutagenesis at Specific Genomic Loci of Amphioxus Branchiostoma belcheri Using TALEN Method

Amphioxus, also called lancelet or cephalochordate, is a promising model organism owning to its particularly evolu- tionary position, simple genome content and comparable body plan to that of vertebrates （Holland et aL, 2004; Bertrand and Escriva, 2011）. However, use of amphioxus as a model or- ganism has been limited for many years because of lack of an efficient genomic modification method. Recently, several revolutionary gene targeting methods that could induce directed mutations, insertions and deletions at intended target sites, have been developed （Gaj et al., 2013）.     

第13届国际生物奥林匹克竞赛(2002)实验试题答案

(续 2 0 0 3年第 38卷第 6期第 5 7页 )实验 动物系统学和形态学Q1.　 (5分 )A B C D E F G H I J0 1 0 1 0 2 0 4 0 1 0 1 0 1 0 1 0 5 0 3Q2　 (5分 )A B C D E F G H I J0 1 0 2 0 4 0 5 0 1 0 6 0 3 0 70 80 9Q3　 (10分 )A B C D E F G H I JK + + +L +M ++ +N +O +P +R ++S ++T ++ +++ +Q4 A　 (4分 ) 　□　□　　 　□　□Q4 B　 (1分 )　　□Q5　 (5分 )A B C D E F G H I J0 3 0 1 0 2 0 2 0 3 0 3 0 1 0 1 0 3 0 2Q6　 (10分 )　　 A.Gammaruspulex(钩虾 )　 B.Anopheles sp.(疟　蚊 )　 C.H irudo …     

Genetic analysis and gene mapping of a rice few-tillering mutant in early backcross populations (Oryza sativa L.)

A rice mutant, G069, characteristic of few tiller numbers, was found in anther culture progeny from the F1 hybrid between an indica-japonica cross, Gui630×02428. The mutant has another two major features: delayed tillering development and yellowing apex and margin on the mature leaves. As a donor parent, G069 was further backcrossed with the recurrent parent, 02428, for two turns to develop a BC2F2 population. Genetic analysis in the BC2F2 population showed that the traits of few-tillering and yellowing apex and margin on the mature leaves were controlled by one recessive gene. A pool of equally mixed genomic DNA, from few-tillering individual plants in BC2F2, was constructed to screen polymorphism with simple sequence repeat (SSR) markers in comparison with the 02428 genome. One SSR marker and three restriction fragment length polymorphism (RFLP) markers were found possibly linked with the recessive gene. By using these markers, the gene of few-tillering was mapped on chromosome 2 between RFLP marker C     

华南虎栖息地适宜性评价及其保护规划

1 引言地理信息系统(GIS)是对空间数据进行采集、存储检索、转换、运算、显示和分析的计算机系统,是在近二十年来发展起来的(Wikinson G.G.and P.E Fisher,1987)。但是它已在地学、资源与环境等领域中发挥出重要作用。国际上,GIS在动物栖息地研究中的应用已有许多报导(EngM.A.,1991;Kangas J.et al,1993;Kauz R.S.,1992;Michael E.Hodgson,1988;Norton T.W.,1991;Shaw D.M.and S.F.Atkinson,1988;Tappan G.G.,1991;Yonzon P.and R.Jones,1991),在国内,这方面的报道不多(欧阳志云等,1995)。本文介绍运用GIS评价梅花山自然保护区华南虎栖息地适     

Effective inhibition of human cytomegalovirus gene expression by DNA-based external guide sequences

To investigate whether a 12 nucleotide DNA-based miniEGSs can silence the expression of human cytomegalovirus （HCMV） UL49 gene efficiently, A HeLa cell line stably expressing UL49 gene was constructed and the putative miniEGSs （UL49-miniEGSs） were assayed in the stable cell line. Quantitative RT-PCR and western blot results showed a reduction of 67% in UL49 expression level in HeLa cells that were transfected with UL49-miniEGSs. It was significantly different from that of mock and control miniEGSs （TK-miniEGSs） which were 1 and 7%, respectively. To further confirm the gene silence directed by UL49-miniEGSs with human RNase P, a mutant of UL49-miniEGSs was constructed and a modified 51 RACE was carried out. Data showed that the inhibition of UL49 gene expression directed by UL49-miniEGSs was RNase P-dependent and the cleavage of UL49 mRNA by RNase P was site specific. As a result, the length of DNA-based miniEGSs that could silence gene expression efficiently was only 12 nt. That is significantly less than any other oligonucleotide-based method of gene inactivation known so far. MiniEGSs may represent novel gene-targeting agents for the inhibition of viral genes and other human disease related gene expression.     

Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage

To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat(SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum×Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centi Morgan(c M) of the cotton AD genome, with an average marker interval of 2.23 c M. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci(QTLs) for lint percentage(LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%–2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality.     

新疆碱茅属的新分类群

发表了禾本科碱茅属的三个新种:多花碱茅Puccinellia florida D.F.Cui,sp.nov.,阿尔金山碱茅P.arjinshanensis D.F.Cui,sp.nov.,小药碱茅P.microanthera D.F.Cui,sp.nov.。     

DGMP: Identifying Cancer Driver Genes by Jointing DGCN and MLP from Multi-omics Genomic Data

Identification of cancer driver genes plays an important role in precision oncology research, which is helpful to understand cancer initiation and progression. However, most existing computational methods mainly used the protein–protein interaction(PPI) networks, or treated the directed gene regulatory networks(GRNs) as the undirected gene–gene association networks to identify the cancer driver genes, which will lose the unique structure regulatory information in the directed GRNs, and then affe...     

Mitochondrial haplogroups associated with Japanese centenarians,Alzheimer＇s patients,Parkinson＇s patients,type 2 diabetic patients and healthy non-obese young males

The relationships between five classes of Japanese people （i.e., 96 centenarians, 96 Alzheimer＇s disease （AD） patients, 96 Parkinson＇s disease （PD） patients, 96 type 2 diabetic （T2D） patients, and 96 healthy non-obese young males） and their mitochondrial single nucleotide polymorphism （mtSNP） frequencies at individual mtDNA positions of the entire mitochondrial genome were examined using the radial basis function （RBF） network and the modified method. New findings of mitochondrial haplogroups were obtained for individual classes. The five classes of people were associated with the following haplogroups： Japanese centenarians-M7b2, D4b2a, and B5b; Japanese AD patients-G2a, B4cl, and N9b1; Japanese PD patients-M7b2, B4e, and B5b; Japanese T2D patients-B5b, M8al, G, D4, and F1; and Japanese healthy non-obese young males-D4g and D4b1b. From the points of common haplogroups among the five classes, the cente- narians have the common haplogroups M7b2 and B5b with the PD patients and common haplogroup B5b with the T2D patients. In addition, the 112 Japanese semi-supercentenarians （over 105 years old） recently reported were also examined by the method proposed. The results obtained were the haplogroups D4a, B4c1a, M7b2, F1, M1, and B5b. These results are different from the previously reported haplogroup classifications. As the proposed analysis method can predict a person＇s mtSNP constitution and the probabilities of becoming a centenarian, AD patient, PD patient, or T2D patient, it may be useful in initial diagnosis of various diseases.