共查询到20条相似文献,搜索用时 46 毫秒
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Ariadna Peremarti Ludovic Bassie Paul Christou Teresa Capell 《Plant molecular biology》2009,70(3):253-264
Polyamines are known to play important roles in plant stress tolerance but it has been difficult to determine precise functions
for each type of polyamine and their interrelationships. To dissect the roles of putrescine from the higher polyamines spermidine
and spermine, we generated transgenic rice plants constitutively expressing a heterologous S-adenosylmethionine decarboxylase (SAMDC) gene from Datura stramonium so that spermidine and spermine levels could be investigated while maintaining a constant putrescine pool. Whereas transgenic
plants expressing arginine decarboxylase (ADC) produced higher levels of putrescine, spermidine and spermine, and were protected from drought stress, transgenic plants
expressing SAMDC produced normal levels of putrescine and showed drought symptoms typical of wild type plants under stress,
but the transgenic plants showed a much more robust recovery on return to normal conditions (90% full recovery compared to
25% partial recovery for wild type plants). At the molecular level, both wild type and transgenic plants showed transient
reductions in the levels of endogenous ADC1 and SAMDC mRNA, but only wild type plants showed a spike in putrescine levels under stress. In transgenic plants, there was no spike
in putrescine but a smooth increase in spermine levels at the expense of spermidine. These results confirm and extend the
threshold model for polyamine activity in drought stress, and attribute individual roles to putrescine, spermidine and spermine. 相似文献
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The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes, ACT1, ACT2, GAPDH, eIF1, eIF2, α-TUB, β-TUB, TBP, RNA Pol II and RP II, were calculated for a suitable reference gene selection in Paeonia ostii T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one. In addition, the geNorm program provided the optimal choice of two reference gene combination, RNA Pol II and β-TUB, for qRT-PCR normalization in P. ostii subjected to different drought stress levels. Our research provided convenience for gene expression analysis in P. ostii under drought stress and promoted research of effective methods to alleviate P. ostii drought stress in the future. 相似文献
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Shoukai Lin Shichang Xu Liyan Huang Fuxiang Qiu Yihong Zheng Qionghao Liu Shiwei Ma Bisha Wu Jincheng Wu 《Phyton》2023,92(4):1185-1201
Loquat (Eriobotrya japonica Lindl.) is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components. Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression. In this study, eight candidate reference genes were selected from our previously published RNA-seq data, and primers for each candidate reference gene were designed and evaluated. The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples, including 12 subgroups of developing or abiotic-stressed tissues. Different combinations of stable reference genes were screened according to a comprehensive rank, which was synthesized from the results of four algorithms, including the geNorm, NormFinder, BestKeeper and ΔCt methods. The screened reference genes were verified by normalizing EjLGA1 in each subgroup. The obtained suitable combinations of reference genes for accurate normalization were GAPDH, EF1α and ACT for floral development; GAPDH, UBCE and ACT for fruit setting; EF1α, GAPDH and eIF2B for fruit ripening; ACT, EF1α and UBCE for leaves under heat stress; eIF2B, UBCE and EF1α for leaves under freezing stress; EF1α, TUA and UBCE for leaves under salt stress; ACT, EF1α and eIF2B for immature pulp under freezing stress; ACT, UBCE and eIF2B for immature seeds under freezing stress; EF1α, eIF2B and UBCE for both immature pulp and seeds under freezing stress; UBCE, TUB and TUA for red-fleshed fruits under cold-storage stress; eIF2B, RPS3 and TUB for white-fleshed fruits under cold-storage stress; and eIF2B, UBCE and RPS3 for both red- and white-fleshed fruits under cold-storage stress. This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat. To our knowledge, this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E. japonica. The use of the three most stable reference genes could increase the reliability of future quantification experiments. 相似文献
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Jing Qu Shuang Liu Peng Jiao Zhenzhong Jiang Jianbo Fei Shuyan Guan Yiyong Ma 《Phyton》2022,91(8):1709-1719
To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (ICE1) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize (Zea mays L.) genomes of the three T1 generations. Under low temperature-stress conditions (4°C), the relative conductivity levels decreased by 27.51%–31.44%, the proline concentrations increased by 12.50%–17.50%, the malondialdehyde concentrations decreased by 16.78%–18.37%, and the peroxidase activities increased by 19.60%–22.89% in the T1 lines compared with those of the control. A real-time quantitative PCR analysis showed that the ICE1 gene was ectopically expressed in the roots, stems, and leaves of the T1 lines. ICE1 positively regulates the expression of the CBF genes in response to cold stress. Thus, this study showed the successful transformation of maize with the ICE1 gene, resulting in the generation of a new maize germplasm that had increased tolerance to cold stress. 相似文献
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Analía I Alet Diego H Sanchez Juan C Cuevas Secundino del Valle Teresa Altabella Antonio F Tiburcio Francisco Marco Alejandro Ferrando Fabiana D Espasandín María E González Pedro Carrasco Oscar A Ruiz 《Plant signaling & behavior》2011,6(2):278-286
Polyamines have been globally associated to plant responses to abiotic stress. Particularly, putrescine has been related to a better response to cold and dehydration stresses. It is known that this polyamine is involved in cold tolerance, since Arabidopsis thaliana plants mutated in the key enzyme responsible for putrescine synthesis (arginine decarboxilase, ADC; EC 4.1.1.19) are more sensitive than the wild type to this stress. Although it is speculated that the overexpression of ADC genes may confer tolerance, this is hampered by pleiotropic effects arising from the constitutive expression of enzymes from the polyamine metabolism. Here, we present our work using A. thaliana transgenic plants harboring the ADC gene from oat under the control of a stress-inducible promoter (pRD29A) instead of a constitutive promoter. The transgenic lines presented in this work were more resistant to both cold and dehydration stresses, associated with a concomitant increment in endogenous putrescine levels under stress. Furthermore, the increment in putrescine upon cold treatment correlates with the induction of known stress-responsive genes, and suggests that putrescine may be directly or indirectly involved in ABA metabolism and gene expression.Key words: cold acclimation, dehydration, putrescine, polyamines, stress 相似文献
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Iris domestica is a plant of the Iridaceae family and is drought-tolerant, but its drought-resistance mechanism is not yet clear. Analysing the gene expression changes of I. domestica by qRT-PCR is an important mean to understand its drought resistance characteristics. Nevertheless, a lack of reference genes greatly hinders investigation and research on the adaptation of I. domestica to drought at the molecular and genetic levels. In this study, we assessed the expression stability of 11 candidate gene in I. domestica under drought stress conditions and different tissues using geNorm, NormFinder, BestKeeper and RefFinder tools. The results showed that EF1β was the most stable reference genes under drought stress and in different tissues. To validate further the stability of the identified reference genes, the expression patterns of VP gene in I. domestica was analysed. These results will be conducive to more accurate quantification of gene expression levels in I. domestica. 相似文献
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Dazhuang Qi Meixia Liang Fudong Jiang Jianzhao Li Xuqiang Qiao Hongxia Zhang 《Phyton》2020,89(4):943-955
Manganese (Mn) is one of the essential microelements in all organisms.
However, high level of Mn is deleterious to plants. In this study, the effects of exogenous manganese application on mineral element, polyamine (PA) and antioxidant
accumulation, as well as polyamine metabolic and antioxidant enzyme activities,
were investigated in Malus robusta Rehd., a widely grown apple rootstock. High
level of Mn treatments decreased endogenous Mg, Na, K and Ca contents, but
increased Zn content, in a Mn-concentration-dependent manner. Polyamine metabolic assays revealed that, except the content of perchloric acid insoluble bound
(PIS-bound) spermine, which increased significantly, the contents of putrescine
(Put), spermidine (Spd) and spermine (Spm) all decreased progressively, accompanied with the decreased activities of arginine decarboxylase (ADC, EC 4.1.1.19)
and ornithine decarboxylase (ODC, EC 4.1.1.17), and the increased activities of
diamine oxidase (DAO, EC 1.4.3.6) and polyamine oxidase (PAO, EC 1.5.3.3).
Further antioxidant capacity analyses demonstrated that contents of anthocyanin,
non-protein thiols (NPT) and soluble sugar, and the activities of guaiacol peroxidase
(POD, EC 1.11.1.7), catalase (CAT, EC 1.11.1.6) and superoxide dismutase (SOD,
EC 1.15.1.1), also increased upon different concentrations of Mn treatments. Our
results suggest that endogenous ion homeostasis is affected by high level of Mn
application, and polyamine and antioxidant metabolism is involved in the responses
of M. robusta Rehd. plants to high level of Mn stress. 相似文献
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Shiyang Li Xue-Ao Liu Lizi Zhao Huiqing Huang Bei Li Zhizhong Song Meixia Liang Hongxia Zhang Limin Wang Shenglin Zhou 《Phyton》2021,90(3):761-772
Seven in absentia (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a seven in absentia gene IbSINA5 was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that IbSINA5 was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm and nucleus. Overexpression of IbSINA5 in sweet potato led to dramatically improved resistance to cold stress in transgenic plants, which was associated with the up-regulated expression of IbCOR (cold-regulated) genes, increased proline production, and decreased malondialdehyde (MDA) and H2O2 accumulation in the leaves of transgenic plants. Furthermore, transient expression of IbCBF3, a C-repeat binding factor (CBF) gene, in the leaf protoplasts of wild type sweet potato plants up-regulated the expression of both IbSINA5 and IbCOR genes. Our results suggest that IbSINA5 could function as a positive regulator in the cold signaling pathway through a CBF-SINA-COR mediated module in sweet potato, and have a great potential to be used as a candidate gene for the future breeding of new plant species with improved cold resistance. 相似文献
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Minghui Niu Heng Zhang Xiangyang Li Zhibao Hu Hongjiao Zhang Zhiru Xu Chunpu Qu Guanjun Liu 《Phyton》2023,92(7):2001-2015
KANADI (KAN) is a plant-specific gene that controlled the polarity development of lateral organs. It mainly acted on the abaxial characteristics of plants to make the lateral organs asymmetrical. However, it had been less identified in woody plants. In this study, the members of the KAN gene family in Populus trichocarpa were identified and analyzed using the bioinformatics method. The results showed that a total of 8 KAN family members were screened out, and each member contained the unique GARP domain and conserved region of the family proteins. Phylogenetic analysis and their gene structures revealed that all KAN genes from P. trichocarpa, Arabidopsis thaliana, and Nicotiana benthamiana could be divided into four subgroups, while the eight genes in P. trichocarpa were classified into three subgroups, respectively. The analysis of tissue-specific expression indicated that PtKAN1 was highly expressed in young leaves, PtKAN6 was highly expressed in young leaves and mature leaves, PtKAN2, PtKAN5, and PtKAN7 were highly expressed in nodes and internodes, PtKAN8 was highly expressed in roots, and PtKAN3 and PtKAN4 showed low expression levels in all tissues. Among them, PtKAN2 and PtKAN6, and PtKAN4 and PtKAN5 might have functional redundancy. Under high nitrogen concentrations, PtKAN2 and PtKAN8 were highly expressed in mature stems and leaves, respectively, while PtKAN4, PtKAN5, and PtKAN7 were highly expressed in roots. This study laid a theoretical foundation for further study of the KAN gene-mediated nitrogen effect on root development. 相似文献
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Hongxiu Gao Lin Zhu Tianqi Liu Xueyu Leng Zhenxing Zhu Wei Xie Haitao Lv Zhengxun Jin Ping Wu Zhongchen Zhang 《Phyton》2023,92(6):1743-1763
Cyclophilin (CYP) plays an important role in plant response to stress, and OsCYP2, one gene of cyclophlilin
family, is involved in auxin signal transduction and stress signaling in rice. However, the mechanism that OsCYP2
is involved in rice response to low temperature is still unclear. We identified a new OsCYP2 allelic mutant, lrl3,
with fewer lateral roots, and the differences in shoot height, primary root length and adventitious root length
increased with the growth process compared to the wild-type plant. Auxin signaling pathway was also affected
and became insensitive to gravity. The transgenic rice plants with over-expression of OsCYP2 were more tolerant
to low temperature than the wild-type plants, suggesting that OsCYP2 was involved in the low temperature
response in rice. In addition, OsCYP2 negatively regulated the expression of OsTPS38, a terpene synthase gene,
and was dependent on the OsCDPK7-mediated pathway in response to low temperature stress. OsTPS38-
overexpressed transgenic line ox-2 was more sensitive to low temperature. Therefore, OsCYP2 may negatively
regulate OsTPS38 through an OsCDPK7-dependent pathway to mediate the response to low temperature in rice.
These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress. 相似文献
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EARLY FLOWERING 3 (ELF3), a light zeitnehmer (time-taker) gene, regulates circadian rhythm and photoperiodic flowering in Arabidopsis, rice, and barley. The three orthologs of ELF3 (TaELF3-1AL, TaELF3-1BL, and
TaELF3-1DL) have been identified in wheat too, and one gene, TaELF3-1DL, has been associated with heading
date. However, the basic characteristics of these three genes and the roles of the other two genes, TaELF3-1BL
and, TaELF3-1AL, remain unknown. Therefore, the present study obtained the coding sequences of the three
orthologs (TaELF3-1AL, TaELF3-1BL, and TaELF3-1DL) of ELF3 from bread wheat and characterized them
and investigated the role of TaELF3-1BL in Arabidopsis. Protein sequence comparison revealed similarities among
the three TaELF3 genes of wheat; however, they were different from the Arabidopsis ELF3. Real-time quantitative
PCR revealed TaELF3 expression in all wheat tissues tested, with the highest expression in young spikes; the three
genes showed rhythmic expression patterns also. Furthermore, the overexpression of the TaELF3-1BL gene in
Arabidopsis delayed flowering, indicating their importance in flowering. Subsequent overexpression of
TaELF3-1BL in the Arabidopsis ELF3 nonfunctional mutant (elf3 mutant) eliminated its early flowering phenotype, and slightly delayed flowering. The wild-type Arabidopsis overexpressing TaELF3-1BL demonstrated
reduced expression levels of flowering-related genes, such as CONSTANS (AtCO), FLOWERING LOCUS T (AtFT),
and GIGANTEA (AtGI). Thus, the study characterized the three TaELF3 genes and associated TaELF3-1BL with
flowering in Arabidopsis, suggesting a role in regulating flowering in wheat too. These findings provide a basis for
further research on TaELF3 functions in wheat. 相似文献
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Zhenfei Guo Jiali Tan Chunliu Zhuo Congying Wang Bin Xiang Zengyu Wang 《Plant biotechnology journal》2014,12(5):601-612