贡嘎山东坡非飞行小型兽类物种多样性的垂直分布格局

为了解贡嘎山地区物种多样性的垂直分布格局,2010年4—9月利用夹日法对贡嘎山东坡非飞行小型兽类的物种多样性进行了详细调查。调查在海拔1200—4000m之间按400m间隔设置了8个采集样地,累计布夹28800夹次,捕获非飞行小型兽类个体701个,观察记录到松鼠个体25个,共调查记录小兽个体726个,分属于3目6科16属25种。非参数估计的物种丰富度Chao2和Jackknife2指数以及物种累积曲线评估表明本次调查取样充分,能很好地反映该地区非飞行小型兽类物种多样性的垂直分布格局。结果表明:非飞行小型兽类物种多样性的垂直分布格局为单峰模型;物种丰富度和物种多度在中海拔地区最高,在低海拔和高海拔地区较低;相反,物种均匀度在中海拔地区较低,在低海拔和高海拔地区较高;而物种优势度则随着海拔的升高而逐渐增加;Shannon-Wiener、Fisher-α、Margalef三个综合性物种多样性指数均显示物种多样性在中海拔地区最高;与其它多样性指数相比,Simpson指数未能很好地反映出不同海拔段群落物种多样性的垂直分布差异;而与Shannon-Wiener和Simpson指数相比,Fisher-α和Margalef指数对不同物种组成的群落多样性区分较好。同时,基于不同海拔段物种组成的聚类分析结果也表明物种多样性在中海拔地区最高。物种多样性在中海拔地区最高的垂直分布模式提示我们在贡嘎山地区的生物多样性保护和生态管理中应特别重视中海拔地段,因为该地段中居于生态食物链中间位置的小兽物种最丰富,是山地生物多样性保护的关键。此外,规范统一的调查方法将有利于研究数据的整合和减少人为因素带来的误差。     

广州不同生境类型区域昆虫多样性

2005—2006年,对广州全域4个不同生境类型区域(中心城市区、农田区、沿海湿地区和森林区)的昆虫群落进行6次调查,共获得昆虫标本10595号,分属于22个目、216个科。多样性分析结果表明,森林区昆虫群落的丰富度指数、多样性指数、复杂性指数最高,其次为农田区、中心城市区,最低为沿海湿地区;沿海湿地区昆虫群落的优势度最高,其他依次为中心城市区、农田区和森林区。不同生境类型昆虫群落多样性指数的时间动态不同,森林区、农田区和沿海湿地区的丰富度指数及Shannon-Wiener多样性指数均为6—7月最高,中心城市区以9—10月最高;农田区昆虫群落的Shannon-Wiener多样性指数及均匀度指数随时间波动最大,森林区的丰富度指数时间波动最大,中心城市区的优势度时间波动最大。相似性分析结果表明,森林区和农田区昆虫群落具有的相同科数最多,相似性系数最高,沿海湿地区和森林区、农田区、城市区具有的相同科数都较少,相似性系数也较低。广州不同生境类型区域昆虫多样性存在差异,森林区受城市化进程影响较小,生境得到较好保护,昆虫多样性丰富,沿海湿地区由于城市化发展改变了原生生境,昆虫多样性遭到较大破坏,因此在城市化进程中,要加强...     

鹞落坪自然保护区蝽类昆虫区系与多样性

2006～2007年期间对鹞落坪自然保护区蝽类昆虫多样性进行调查,在不同的生境、月份及海拔对该地区的蝽类昆虫种类及数量进行抽样统计,将调查到的物种进行编目,并采用Margalof丰富度指数、Simpson多样性指数、Shannon-Wiener指数、Pielou指数和Whittaker多样性指数对其群落多样性进行分析。结果表明,鹞落坪共有蝽类昆虫102种,分别隶属于13科,77属。其中有33种为安徽省新记录种。区系分析表明该地区东洋种成分占优势,古北种次之,古北、东洋共有种最少。蝽类群落α多样性分析结果显示:不同生境多样性指数高低顺序是:阔叶灌丛、阔叶林、灌草丛、竹林、农田;不同月份多样性指数高低顺序是:5,7,6,4,9和8月;不同海拔多样性指数高低顺序是:1100,800,700,600,900,1000,1200,500和1300m以上。多样性、均匀性及丰富度的相关分析说明:在不同生境、不同月份、不同海拔,鹞落坪蝽类群落多样性H′指数与Simpson多样性指数及丰富度是一致的,H′与均匀度均不一致。鹞落坪蝽类群落β多样性指数受生境影响最大。     

晋中万柏林生态园不同海拔及干扰程度访桃花昆虫群落差异

[目的]明确万柏林生态园不同海拔及干扰程度访桃花昆虫群落组成、丰富度、多度和多样性参数差异。[方法]不同海拔高度,设置不同生境样地,定点定树拍照法采样,利用统计学分析方法,分析万柏林生态园不同海拔及干扰程度分类阶元访桃花昆虫群落物种组成、丰富度、多度及多样性。[结果]经鉴定,万柏林生态园共采集访桃花昆虫126个体,15种;游人汽车干扰强小生境、汽车干扰小生境、游人汽车干扰强生境、汽车干扰生境、游人汽车干扰弱生境、游人干扰生境中各采到访桃花昆虫7种、4种、7种、4种、10种和8种。相同年份内游人汽车干扰强小生境与游人汽车干扰强生境、汽车干扰强生境访桃花昆虫群落极相似(q=1.000),游人汽车干扰强小生境、游人汽车干扰强生境与汽车干扰小生境、汽车干扰生境访桃花昆虫群落极不相似(q=0.100);低海拔样地访桃花昆虫群落丰富度(R=10)和多度(P=46)分别低于高海拔样地访桃花昆虫群落丰富度(R=12)和多度(P=82)。[结论]不同海拔及干扰程度访桃花昆虫群落丰富度、多度和多样性参数存在差异;不同干扰程度桃园生境中特有访桃花昆虫物种可作为生境指示物种;优势访桃花昆虫物种可作为桃树传粉昆虫。万柏林生态园桃树花期与果成熟期,干扰严重,需要大力保护花期与果期桃园。     

湖北三峡库区兴山县昆虫多样性调查与评估

【目的】湖北三峡库区兴山县特有的地理环境与气候条件造就了当地丰富的昆虫资源,本研究对湖北三峡库区特有区域兴山县的昆虫多样性开展调查,评估兴山县昆虫多样性的威胁因子,并提出兴山县昆虫多样性保护建议。【方法】通过GPS定位系统对调查区域进行网格和区域划分,将整个兴山县分为28个有效网格(长和宽各10 km),在2017年的昆虫发生期(6月中旬)、暴发期(8月中旬)和越冬期(10月中旬),采用样线踏查法对兴山县不同生境和不同海拔网格昆虫多样性进行调查和评估。【结果】2017年从湖北省兴山县共采集并鉴定出16个目136科612种昆虫,优势目分别是鳞翅目(Lepidoptera)(47.39%)、鞘翅目(Coleoptera)(22.88%)和半翅目(Hemiptera)(11.44%)。兴山县低海拔网格昆虫多样性指数和丰富度较低,而高海拔网格昆虫多样性指数和丰富度较高。黄柏坪(龙门河林场)的昆虫Shannon-Wiener多样性指数(H′)和Margalef丰富度指数(ds)最高。【结论】兴山县昆虫优势类群为鳞翅目、鞘翅目和半翅目,网格生境中林地及林地-灌木丛混合地更适合昆虫的栖息,植被丰富的...     

长白山北坡不同海拔及干扰程度下访花食蚜蝇群落的差异

本研究采用垂直面取样法,对中国长白山北坡不同海拔及干扰程度下访花食蚜蝇群落的物种组成、多度、丰富度及多样性进行了调查研究。我们选择了不同垂直带的两个样地,分别位于800~1 100 m和1 750 ~2 150 m;每个样地选取3种生境类型,每一生境类型采样面积为20 m×20 m。252组调查数据表明,共采到访花食蚜蝇42种2 540个体。不同海拔生境访花食蚜蝇的组成、多度、丰富度及多样性的差异分析结果表明: 低海拔样地内不同生境访花食蚜蝇的群落相似性高于高海拔样地;低海拔样地访花食蚜蝇的多度高于高海拔样地,而物种丰富度低于高海拔样地;不同生境类型访花食蚜蝇多样性指数存在差异,但高海拔样地与低海拔样地多样性差异不显著;过渡林生境（岳桦林带）与次生林生境（针阔混交林带Ⅱ）中访花食蚜蝇的多样性较高。保护珍稀植物物种的人工植物花园访花食蚜蝇多样性仅次于过渡林生境（岳桦林带）与次生林生境（针阔混交林带Ⅱ）,保护作用显著。     

深圳梧桐山风景区昆虫资源调查及群落结构分析

2012年7月-2013年7月,对深圳梧桐山风景区昆虫资源进行系统调查,并对其群落结构进行分析。期间共采集昆虫标本2934号,鉴定出166种,隶属17目98科。通过对不同生境昆虫物种丰富度和多样性指数统计分析得出,亚热带常绿阔叶林昆虫物种多样性指数最高,为3.29;南亚热带常绿灌丛昆虫多样性指数最低,为1.88。各种生境昆虫物种多样性指数从大到小排列为:亚热带常绿阔叶林南亚热带针阔叶混交林南亚热带人工常绿阔叶林南亚热带常绿灌丛。不同生境昆虫群落相似性系数在0.0889-0.2690之间,随着植被构成和环境小气候的差别越大相似性系数越小,说明梧桐山风景区各个生境之间昆虫群落具有一定的独立性。通过对不同垂直地带昆虫物种多样性指数统计分析得出,海拔介于300-600 m地带昆虫物种多样性指数最高,为3.30,海拔900 m以上地带昆虫多样性指数最低,为1.06。不同垂直地带昆虫多样性指数从大到小排列顺序为:海拔300-600 m海拔600-900 m海拔100-300 m海拔900 m以上。     

内蒙古典型草原不同围封年限样地访花昆虫多样性

本文对内蒙古锡林郭勒退化典型草原围封样地,1983年围封禁牧区域(A样地)、1996年围封禁牧区域(B样地)和未围封的自由放牧区(C样地)的访花昆虫群落多样性及时序特征、访花频率进行了研究。结果表明:在3个样地共采集到访花昆虫成虫标本2131头,隶属7目71科,共183种;其中,A样地有7目60科154种1133头,B样地有6目59科158种847头,C样地有3目13科21种151头;A样地访花昆虫的数量最多,B样地访花昆虫的种类最多,C样地访花昆虫的种类与数量均最少;B样地7月份的访花昆虫Shannon多样性指数(3.12)、Pielou均匀度指数(0.86)和Margalef丰富度指数(7.16)均最高;C样地6月份的访花昆虫Berger-Parker优势度指数(0.15)最高。访花昆虫的Shannon多样性指数与Pielou均匀度指数随月份的变化趋势一致,而与Berger-Parker优势度指数相反;通过Jaccard群落相似性系数计算可知,A样地与B样地的访花昆虫Jaccard群落相似性系数(0.25)最高;A样地与C样地的访花昆虫群落相似性系数(0.16)最低;不同样地各月份的优势开花植物的优势访花昆虫不同,访花频率也不同。     

广西猫儿山沿海拔梯度的叶蜂多样性

在猫儿山自然保护区沿海拔梯度设置7个样地进行叶蜂调查.共采获叶蜂803头,隶属3科58属121种.统计分析表明,异鳞纹眶叶蜂(Claremontia sinobirmana Malaise)和南华平缝叶蜂(Nesoselandria southaWei)为优势种和广布种.不同海拔叶蜂群落的物种丰富度指数、Simpson多样性指数和Shannon-Wiener多样性指数均以中间海拔(1000~1500m)最高,分别为12.293、0.971和3.830;优势度指数则以高海拔最高.聚类分析显示,7个样地的叶蜂物种可以分为高海拔组(>2000m)和其它海拔组(<2000m).相关分析表明,降水量和植被种类是影响叶蜂多样性的主要因素.     

南京老山地区鞘翅目昆虫多样性及区系分析

南京老山地区动植物资源丰富,但迄今尚无对老山鞘翅目昆虫多样性的系统研究。为了解南京老山地区鞘翅目昆虫种类、群落结构及分布状况,于2020年和2021年在老山地区对鞘翅目昆虫种类的多样性进行调查,统计分析鞘翅目昆虫的种类及数量,并采用Shannon-Wiener多样性指数、Pielou均匀度指数、Margalef丰富度指数和Simpson优势集中性指数对其群落多样性进行分析。结果显示,老山地区共鉴定出鞘翅目昆虫23科130属152种,从属种关系来看,该地区鞘翅目昆虫属内以单种为主。区系分析表明,该地区以东洋-古北界共有种和古北种为主,比例分别为46.05%和41.45%,东洋种最少,其比例为2.63%。对多样性、均匀度、丰富度及优势集中性的相关分析表明,老山地区鞘翅目昆虫群落结构稳定,物种丰富。本研究填补了老山地区鞘翅目昆虫资源及多样性调查的空白,为该地区鞘翅目昆虫的深入研究、多样性保护和害虫防治奠定了基础,可以为其它相关类群昆虫的调查、研究提供参考依据。     

The simple-septate basidiomycetes: a synopsis

The simple-septate basidiomycetes comprise more than 8,000 species that show a high morphological and ecological heterogeneity. To gain insight in the phylogenetic relationships within this group, we compared several ultrastructural features such as septal pore apparatus, form, and behavior of the spindle pole bodies, types of host–parasite interaction, presence or absence of colacosomes, symplechosomes, atractosomes, and cystosomes as well as nuclear rDNA sequences coding for small- and large-subunit rRNA. Based on our integrated analysis, we propose a new classification system for the simple-septate basidiomycetes with the subphylum Pucciniomycotina and the classes Agaricostilbomycetes, Atractiellomycetes, Classiculomycetes, Cryptomycocolacomycetes, Cystobasidiomycetes, Microbotryomycetes, Mixiomycetes, and Pucciniomycetes. We also propose the pucciniomycotinous taxa Cystobasidiales, Erythrobasidiales, Helicobasidiales, Mixiales, Naohideales, Pachnocybales, Spiculogloeales, and Kondoaceae and the new subphyla Agaricomycotina (equivalent to the current Hymenomycetes) and Ustilaginomycotina (equivalent to the current Ustilaginomycetes).     

略论棕榈科与新分出的省藤科的系统分类、演化、区系地理及主要的经济用途

棕榈科原省藤亚科因其子房壁及外果皮被倒生、螺旋状排列的鳞片所覆盖,而区别于其他亚科,因而独立分出成一新科--省藤科。作者讨论了棕榈科的祖先种可能在石炭纪时,自原始裸子植物开以顿目在分化、衍生出苏铁目祖先种的进化干上,于白垩纪时分化出的一个分支。在棕榈科的祖先种出现不久后,在其进化的分支上,于白垩纪后期又分化出一旁支,成为棕榈科的姊妹科--省藤科的祖先种。从两祖先种分别再分化、衍生出现今分布地球上该二科的属与种。两科、尤其前者是被子植物、尤其是单子叶植物中最原始的类群之一。作者还提出棕榈科象牙椰亚科与贝叶棕亚科是该科最原始或较原始的两类群;槟榔亚科和腊材榈亚科是较进化的两类群;而水椰亚科祖先种可能源于象牙椰亚科的祖先种,但又演化为该科最进化与特化的类群。省藤科省藤亚科略比鳞果榈亚科原始。作者讨论了两科为泛热带分布的科,指出两科的"现代分布区"在南北两半球热带地区,少数种还延伸分布到两半球暖亚热带、甚至达中亚热带地区,分布区边缘最北达日本中部、中国长江流域及黄河下游的南部,美国加利佛尼亚州与佛罗里达州和地中海北部;最南达智利中部和新西兰南部;而"现代分布中心"在热带与暖亚热带的亚洲,中、南美洲,大洋洲及非洲的东、南、西部;但分布区的"密集中心"则在热带亚洲、热带中及南美洲、南太平洋群岛及非洲东南部。作者还介绍了近50年我国南方引种驯化成功的两科植物近400种(见^*图谱),其中少数为耐寒的种类,有的种已引种到长江流域或更北的地区。引种的大部分种都有其重要的经济用途,包括:1. 食用,如淀粉和树液可制"西米"或制糖,酿酒、醋或作饮料;果或种子榨油,供食用或工业用;某些种的嫩芽作蔬菜,甚至种子代咖啡饮用;2. 药用,有消炎、止血、活血、驱虫、抗癌等用;3. 建筑、工艺与日用品,包括不少种的树干供建普通房子、桥梁、小船;少数种可提制工业用蜡;许多种的纤维制高级缆绳和编织品;还制工艺品与日用品等;4. 代表热带景观的园林工程、绿化及美化环境的观赏树和人行道树及建造园林景观生态类型的树种等。     

Sugars in crop plants

We review current knowledge of the most abundant sugars, sucrose, maltose, glucose and fructose, in the world's major crop plants. The sucrose‐accumulating crops, sugar beet and sugar cane, are included, but the main focus of the review is potato and the major cereal crops. The production of sucrose in photosynthesis and the inter‐relationships of sucrose, glucose, fructose and other metabolites in primary carbon metabolism are described, as well as the synthesis of starch, fructan and cell wall polysaccharides and the breakdown of starch to produce maltose. The importance of sugars as hormone‐like signalling molecules is discussed, including the role of another sugar, trehalose, and the trehalose biosynthetic pathway. The Maillard reaction, which occurs between reducing sugars and amino acids during thermal processing, is described because of its importance for colour and flavour in cooked foods. This reaction also leads to the formation of potentially harmful compounds, such as acrylamide, and is attracting increasing attention as food producers and regulators seek to reduce the levels of acrylamide in cooked food. Genetic and environmental factors affecting sugar concentrations are described.     

Physico-chemical environment in Tjeukemeer with special reference to speciation of algal nutrients

This paper attempts to generalize the major controlling factors and their impacts on the physico-chemical environment in Tjeukemeer in relation to the speciation of algal nutrients.Morphometry, man-made hydrology, climate, chemistry of drainage area and biology of the lake appear to control its physico-chemistry the most. Thus the shallow Tieukemeer is vulnerable to wind and therefore almost always completely mixed and often turbid owing to resuspended sediments. In normal summers the lake is oligohaline, hard, eutrophic and alkaline, and in winter it is hard, eutrophic, alkaline and humus-rich. The implications of these properties on the following physico-chemical components and their relevance for the speciation of algal nutrients are discussed: turbidity, colour, halinity, pH, alkalinity, salinity, conductivity, ionic composition, ionic balance and organic matter.Because of the relationships between the physico-chemical environment on the one hand and the speciation and bio-availability of nutrients on the other hand, special attention is given to the speciation of some major and minor elements as derived from ultra filtration experiments. In this context, the metal binding capacity of the organic matter (mainly fulvic acids) is also considered.     

The role of the microbiome in the neurobiology of social behaviour

Microbes colonise all multicellular life, and the gut microbiome has been shown to influence a range of host physiological and behavioural phenotypes. One of the most intriguing and least understood of these influences lies in the domain of the microbiome's interactions with host social behaviour, with new evidence revealing that the gut microbiome makes important contributions to animal sociality. However, little is known about the biological processes through which the microbiome might influence host social behaviour. Here, we synthesise evidence of the gut microbiome's interactions with various aspects of host sociality, including sociability, social cognition, social stress, and autism. We discuss evidence of microbial associations with the most likely physiological mediators of animal social interaction. These include the structure and function of regions of the ‘social' brain (the amygdala, the prefrontal cortex, and the hippocampus) and the regulation of ‘social’ signalling molecules (glucocorticoids including corticosterone and cortisol, sex hormones including testosterone, oestrogens, and progestogens, neuropeptide hormones such as oxytocin and arginine vasopressin, and monoamine neurotransmitters such as serotonin and dopamine). We also discuss microbiome‐associated host genetic and epigenetic processes relevant to social behaviour. We then review research on microbial interactions with olfaction in insects and mammals, which contribute to social signalling and communication. Following these discussions, we examine evidence of microbial associations with emotion and social behaviour in humans, focussing on psychobiotic studies, microbe–depression correlations, early human development, autism, and issues of statistical power, replication, and causality. We analyse how the putative physiological mediators of the microbiome–sociality connection may be investigated, and discuss issues relating to the interpretation of results. We also suggest that other candidate molecules should be studied, insofar as they exert effects on social behaviour and are known to interact with the microbiome. Finally, we consider different models of the sequence of microbial effects on host physiological development, and how these may contribute to host social behaviour.     

农林业系统沙兰杨-小麦、玉米群落类型氮、磷、钾营养元素循环的研究

农林业系统沙兰杨-小麦、玉米群落类型N、P、K营养元素循环研究结果表明:在整个群落中,元素N和P发生损亏,K基本平衡。在植物组分库和枯落物库中,元素N、P、K均发生累积;在土壤库中,元素N、P和K均发生亏损。土壤表层(0—20cm)N的含量是限制农作物正常生长发育的主要环境因子。土壤库中有效N和速效P的含量是限制沙兰杨正常生长发育的主要环境因子。群落的年输入输出量大,枯落物少,营养元素在各组分间的循环量大等是该群落类型营养元素循环的重要特性。     

Simultaneous accumulation of calcium, phosphorus, and magnesium in various human arteries

To elucidate compositional changes of the arteries with aging, the authors investigated the relationships among average contents of calcium, phosphorus, sulfur, and magnesium in the arteries by inductively coupled plasma-atomic emission spectrometry. The arteries used were the thoracic and abdominal aortas, coronary, common carotid, anterior, middle and posterior cerebral, vertebral, basilar, internal thoracic, axillary, radial, truncus celiacus, common, internal and external iliac, femoral, popliteal, and umbilical arteries. It was found that high correlations were found between the average contents of calcium and phosphorus, between the average contents of calcium and magnesium, and between the average contents of phosphorus and magnesium in the arteries, but not between the average contents of sulfur and the other elements. These correlations revealed that as the content of calcium and phosphorus increased in the arteries, the magnesium content increased simultaneously in the arteries, but the sulfur content did not. It is likely that magnesium forms compounds with phosphorus in the arteries.     

The morphology and relationships of the Carboniferous amphibian Eoherpeton watsoni Panchen

The oldest recorded anthracosaur amphibian Eoherpeton watsoni Panchen, from localities in the Visean and Namurian of the Scottish Carboniferous, is described. It lacks a distinct otic notch, and reconstruction of the position of cranial ligaments and muscles suggest that a tympanum was never developed. On the basis of these observations it is concluded that arguments used to refute a relationship between anthracosaurs and amniotes, based on apparent differences in the morphology of their otic regions, are invalid. The order Anthracosauria is considered to be a monophyletic group characterized by the development of a sutural connection between the tabular and parietal bones on a skull table which includes both the intertemporal and supratemporal as well as the tabular. It is divided into two suborders: Seymouriamorpha characterized by a sutural connection between the squamosal and intertemporal and the presence of a lateral otic tube, and Anthracosauroideae characterized by the absence of post-temporal fossae. The Discosauriscidae, Kotlassiidae and Seymouriidae are included in the Seymouriamorpha, and the Eoherpetontidae, Gephyrostegidae and Embolomeri comprise the Anthracosauroideae. The Proterogyrinidae are considered the most primitive family of embolomeres. A new phylogeny of Amphibia is presented. The old groupings of Labyrinthodontia, Lepospondyli and Lissamphibia are rejected and Amphibia is divided, on the basis of differences in morphology of the neurocranium and its relationship with the surrounding bones of the skull, into two major groups, the first including the Ichthyostcgalia, Temnospondyli, Microsauria and the extant Anura, Apoda and Urodela, while the Aistopoda, Nectridea, Palaeostegalia, Loxommatoidea, Anthracosauria and Diadectomorpha, together with the Amniota, form the second. Similarities in the dentition of the palaeostegalian Crassigyrinus and loxommatoids suggest the two taxa are sister-groups, but the immediate relationships of anthracosaurs remain indeterminate and they here form an unresolved trichotomy with diadectomorphs and amniotes.     

IPBES工作效率和科学职能的有效性分析

IPBES第一轮工作方案受到国际社会的广泛关注,奠定了其作为第一个生物多样性领域政府间机制的重要地位.IPBES自成立以来相继发布了系列评估报告和决策者摘要,引起了国际社会广泛关注,因此,其公平性、公正性、科学性和透明性始终为各界关注的焦点,直接决定着IPBES评估报告的可信度和未来的发展.为有效提升工作效率,提高其成...     

Structural activity relationship between Salmonella-mutagenicity and nitro-orientation of nitroazaphenanthrenes

Nitroazaphenanthrenes (NAphs) and their N-oxides (NAphOs) were synthesized as derivatives with nitrogen atoms in the 1, 4, and 9 positions of phenanthrene rings, and as nitrated derivatives substituted at the 1, 2, 3, 4, 5, 6, 7, and 8 positions of phenanthrene rings. To determine the structure activity relationship of these derivatives, all 19 isomers were bioassayed with Salmonella tester strains. NAphs substituted at the 4, 6, 7 and 8 positions were mutagenic for TA98, and 1-, 2-, and 3-N-9-AphOs, 6-N-1-AphO and 6-N-4-AphO were mutagenic for TA98 and TA100 without the S9 mix, while 5-N-1-AphO and 5-N-9-AphO were non- or weakly mutagenic. Nitrated derivatives, 6-N-4-Aph, 6-N-9-Aph, 6-N-1-AphO, and 6-N-4-AphO, were powerful mutagens for TA98 and TA100. Mutagenicity was enhanced by mutant strains producing nitroreductase, such as YG1021 and 1026, and by those producing O-acetyltransferase, such as YG1024 and 1029. Nitro derivatives substituted at positions 4 and 5 in the phenanthrene rings were perpendicular, while those at positions 2, 3, 6 and 7 were coplanar to the phenanthrene rings. NAphs substituted at the 1 and 8 positions were noncoplanar due to steric hindrance of the aromatic proton at the peri position. On the other hand, 1,5- and 1,8-dinitro-4-azaphenanthrenes showed high mutagenicity for strains TA98 and TA100 in the absence of the S9 mix, and were strongly enhanced by nitroreductase and O-acetyltransferase, over-producing mutants. Therefore, it was found that the mutagenic potency of NAphs and NAphOs was closely associated with the chemical properties and orientation of nitro substitution of aromatic rings.