The impact of physical training on neutrophil extracellular traps in young male athletes – a pilot study

Neutrophils are an important component of the innate immune response against various pathogens. However, there is a lack of research concerning the effects of short intensive training on neutrophil functions, especially neutrophil extracellular traps (NET) formation. The study aim was to determine the effects of a 19-day training cycle on innate immunity among young male athletes. Six male ice hockey players (< 20 years old) from the Polish national team were monitored across a five-day training camp and after a return to normal club training. The first blood collection took place before training (T1), the second after the training camp (T2) and the third 14 days later (T3). The counts/concentrations of blood biochemical, immune and endocrine markers were compared across each training period. Creatine kinase activity tended to increase at T2 (546 ± 216 U·L^-1) when compared to T1 (191 ± 111 U·L^-1; p=0.063). Neutrophil extracellular traps formation and neutrophil counts also differed between training periods (p=0.042 and p=0.042, respectively). Neutrophil counts tended to decrease, in contrast to NET formation which tended to rise, at T2 in comparison to T1 (2.51 ± 0.45 vs 3.04 ± 0.47 109·L^-1; 24 ± 13 vs 8 ± 15%, respectively). No significant differences in other leucocyte counts were observed. A short period of intensive training was accompanied by some muscle damage and inflammation, as evidenced by CK and NET up-regulation, whilst neutrophil counts were diminished in the blood. Thus, neutrophils and NET could be involved in muscle damage and local inflammatory processes following intensive physical training in young male athletes.     

Resisted sprint training with partner towing improves explosive force and sprint performance in young soccer players – a pilot study

The purpose of this study was to examine the effects of non-resisted (NRS) and partner-towing resisted (RS) sprint training on legs explosive force, sprint performance and sprint kinematic parameters. Sixteen young elite soccer players (age 16.6 ± 0.2 years, height 175.6 ± 5.7 cm, and body mass 67.6 ± 8.2 kg) were randomly allocated to two training groups: resisted sprint RS (n = 7) and non-resisted sprint NRS (n = 9). The RS group followed a six-week sprint training programme consisting of two “sprint training sessions” per week in addition to their usual soccer training. The NRS group followed a similar sprint training programme, replicating the distances of sprints but without any added resistance. All players were assessed before and after training: vertical and horizontal jumping (countermovement jump (CMJ), squat jump (SJ), and 5-jump test (5JT)), 30 m sprint performance (5, 10, and 20 m split times), and running kinematics (stride length and frequency). In the RS group significant (p < 0.05) changes were: decreased sprint time for 0–5 m, 0–10 m and 0–30 m (-6.31, -5.73 and -2.00%; effect size (ES) = 0.70, 1.00 and 0.41, respectively); higher peak jumping height (4.23% and 3.59%; ES = 0.35 and 0.37, for SJ and CMJ respectively); and 5JT (3.10%; ES = 0.44); and increased stride frequency (3.96%; ES = 0.76). In the NRS group, significant (p < 0.05) changes were: decreased sprint time at 0–30 m (-1.34%, ES = 0.33) and increased stride length (1.21%; ES = 0.17). RS training (partner towing) for six weeks in young soccer players showed more effective performances in sprint, stride frequency and lower-limb explosive force, while NRS training improved sprint performance at 0–30 m and stride length. Consequently, coaches and physical trainers should consider including RS training as part of their sprint training to ensure optimal sprint performance.     

Sachet water consumption as a risk factor for cholera in urban settings: Findings from a case control study in Kinshasa,Democratic Republic of the Congo during the 2017–2018 outbreak

BackgroundBehavioural risk factors for cholera are well established in rural and semi-urban contexts, but not in densely populated mega-cities in Sub-Saharan Africa. In November 2017, a cholera epidemic occurred in Kinshasa, the Democratic Republic of the Congo, where no outbreak had been recorded for nearly a decade. During this outbreak, we investigated context-specific risk factors for cholera in an urban setting among a population that is not frequently exposed to cholera.Methodology/Principal findingsWe recruited 390 participants from three affected health zones of Kinshasa into a 1:1 matched case control study. Cases were identified from cholera treatment centre admission records, while controls were recruited from the vicinity of the cases’ place of residence. We used standardized case report forms for the collection of socio-demographic and behavioural risk factors. We used augmented backward elimination in a conditional logistic regression model to identify risk factors.The consumption of sachet water was strongly associated with the risk of being a cholera case (p-value 0.019), which increased with increasing frequency of consumption from rarely (OR 2.2, 95% CI 0.9–5.2) to often (OR 4.0, 95% CI 1.6–9.9) to very often (OR 4.1, 95% CI 1.0–16.7). Overall, more than 80% of all participants reported consumption of this type of drinking water. The risk factors funeral attendance and contact with someone suffering from diarrhoea showed a p-value of 0.09 and 0.08, respectively. No socio-demographic characteristics were associated with the risk of cholera.Conclusions/SignificanceDrinking water consumption from sachets, which are sold informally on the streets in most Sub-Saharan African cities, are an overlooked route of infection in urban cholera outbreaks. Outbreak response measures need to acknowledge context-specific risk factors to remain a valuable tool in the efforts to achieve national and regional targets to reduce the burden of cholera in Sub-Saharan Africa.