Diagnostics of fast processes in laser plasmas after the irradiation of low-density media in the mishen facility

New experimental data on the laser irradiation of low-density porous materials in the Mishen facility are presented and discussed. A wide set of optical and X-ray diagnostics was used to analyze the physical processes in porous media with different microstructures and specific densities of 1–30 mg/cm³ exposed to laser pulses with λ=1.054 µm, τ=3 ns, and I=10¹³–10¹⁴ W/cm². The features of laser absorption and scattering and the processes of energy transfer in porous media were investigated for different average densities, thicknesses, and microstructures of the targets and different incidence angles of the laser beam. It was found that the material microstructure (chaotic or quasi-ordered) significantly affected the formation and dynamics of a plasma produced inside the irradiated samples that model the components of the advanced targets used in inertial confinement fusion research.     

Study of the glow dynamics in a laser-produced plasma jet expanding across the magnetic field

Results are presented from experimental studies of the glow dynamics of a plasma jet generated during the irradiation of a plane aluminum target by an iodine laser pulse with the wavelength 1.315 μm. The laser pulse energy was 330–480 J, the pulse duration was 0.5 ns, and the focal spot diameter was 3 mm, the laser intensity on the target surface being ∼10¹³ W/cm². The jet expanded across an external magnetic field with the strength ∼1 kOe. The residual air pressure in the vacuum chamber was ∼10⁻⁵ Torr. The spatiotemporal behavior of the jet glow was investigated using a nine-frame camera in two mutually perpendicular directions (along and across the magnetic field). The results of measurements indicate azimuthal asymmetry of the jet expansion.     

Water permeability of thin lipid membranes

The osmotic permeability coefficient, P_f, and the tagged water permeability coefficient, P_d, were determined for thin (<100 A) lipid membranes formed from ox brain lipids plus DL-α-tocopherol; their value of approximately 1 x 10^-3 cm/sec is within the range reported for plasma membranes. It was established that P_f = P_d. Other reports that P_f > P_d can be attributed to the presence of unstirred layers in the experimental determination of P_d. Thus, there is no evidence for the existence of aqueous pores in these thin phospholipid membranes. The adsorption onto the membrane of a protein that lowers its electrical resistance by a factor of 10³ was found not to affect its water permeability; however, glucose and sucrose were found to interact with the membrane to modify P_f. Possible mechanisms of water transport across these films are discussed, together with the implications of data obtained on these structures for plasma membranes.     

Stability and variations of plasma parameters in the L-2M stellarator during excitation of the induction current in the regime of ECR plasma heating

Results are presented from experimental studies of variations in the plasma parameters during the excitation of a multiaxis magnetic configuration by the induction current (up to 17 kA) in the basic magnetic configuration of the L-2M stellarator in the regime of ECR heating at a microwave power of ～200 kW (～1 MW m^?3) and an average plasma density of (1–2) × 10¹⁹ m^?3. The current direction was chosen to reduce the net rotational transform (the so-called “negative“ current). The current was high enough for the rotational transform to change its sign inside the plasma column. Computer simulations of the L-2M magnetic structure showed that the surface with a zero rotational transform is topologically unstable and gives rise to magnetic islands, i.e., to a multiaxis magnetic configuration. Magnetic measurements showed that, at negative currents above 10 kA, intense bursts of MHD oscillations with a clearly defined toroidal mode number n = 0 were observed in the frequency range of several kilohertz. Unfortunately, the experimental data are insufficient to draw the final conclusion on the transverse structure of these oscillations. The radial temperature profiles along the stellarator major radius in the equatorial plane were studied. It is found that the electron temperature decreases by a factor of 1.3 in the plasma core (r/a ≤ 0.6) and that the temperature jump is retained near the boundary. A change in turbulent fluctuations of the plasma density during the excitation of a negative current was studied using wave scattering diagnostics. It is found that the probability density function of the increments of fluctuations in the plasma core differs from a Gaussian distribution. The measured distribution is heavy-tailed and broadens in the presence of the current. It is found that the spectrum of turbulent fluctuations and their Doppler shift near the plasma boundary are nonuniform in the radial direction. This may be attributed to the shear of the poloidal velocity. The experimental results indicate that the formation of regions with a zero rotational transform in the plasma core somewhat intensifies plasma transport.     

Solution Structure of a Parallel Left-handed Double-helical Gramicidin-A Determined by 2D1H NMR

The structure of a parallel left-handed double-helical form of gramicidin was detected by circular dichroism spectroscopy and determined using 500 and 600 MHz NMR in CaCl₂/methanol solution. Measurements of TOCSY, DQF-COSY and NOESY spectra were converted into 604 distance and 48 torsional angle constraints for structure calculations. Stereospecific assignments and χ₁angles were calculated using³J_αβ, d_αβ(i,i), d_Nβ(i,i) and d_Nγ(i,i). χ₂angles were determined using d_αβ(i,i), d_Nβ(i,i), d_βδ(i,i), d_Nγ(i,i) and d_αγ(i,i). The calculations of initial structures were performed using the distance geometry/simulated annealing method in XPLOR. The initial structures were further refined and energy minimized using simulated annealing/molecular dynamics methods. Back-calculations for every generated structure were also performed to check their consistency with the experimental data.187 final structures with no violations above the threshold conditions (0.05 Å, 5°, 5°, 0.5 Å and 5° for bonds, angles, improper, NOE and cdihe, respectively) were produced from the 200 initial structures. Twenty structures with the lowest NOE energies were used for further analysis. The average r.m.s. deviations for the 20 structures are 0.64 Å for backbone and 1.1 Å for all non-hydrogen atoms.Gramicidin in this form, with approximately 5.7 residues per turn, is a parallel double helical dimer. The length along the helix axis is about 30 Å and the inner pore diameter varies from 1 to 2 Å. It is different from all other gramicidin structures determined to date. The presence of Ca^{2 +}stabilises a conformation that prevents the binding of monovalent cations. It is likely that this structure is related to a non-channel, antibiotic role of gramicidin.     

Study of the fine structure of the plasma current sheath and magnetic fields in the axial region of the PF-1000 facility

Results of measurements of magnetic fields in the plasma pinching region during the compression of the deuterium plasma current sheath (PCS) at the PF-1000 plasma focus facility are presented. The fine structure of the PCS (shock wave-magnetic piston) and its variations in the course of plasma compression toward the facility axis are studied using magnetic probes and laser interferometry. The radial distributions of the plasma density and current in the PCS are compared. It is shown that, in the shock wave region, the electron density of the compressed plasma is on the order of ～10¹⁸ cm^?3, whereas the PCS current is almost entirely concentrated in the magnetic piston region—a plasma layer with an electron density of less than 10¹⁵ cm^?3. Efficient transportation of the current by the PCS into the axial region of the facility in discharges with a high neutron yield (Y _n > 10¹¹ neutrons/shot) is detected. It is shown that the total neutron yield is well described by the dependence Y _n ≈ (1.5–3) × 10¹⁰ I _p ⁴ , where I _p is the pinch current (in MA) flowing within the region r ≤ 13 mm.     

Single-cell measurements of two-dimensional binding affinity across cell contacts

The two-dimensional (2D) affinity between protein molecules across contacting cells is a key parameter regulating and initiating several cellular processes. However, measuring 2D affinity can be challenging, and experimental data are limited. In addition, the obtained 2D affinities are typically averaged over the cell population. We here present a method to measure 2D affinity on single cells binding to polyhistidine-tagged fluorescent ligands anchored to a supported lipid bilayer (SLB). By decreasing the density of ligands in the SLB using imidazole, a new steady-state accumulation in the contact is obtained, and from this change, both the 2D affinity and the number of receptors on the cell can be determined. The method was validated on an SLB containing rat CD2 binding to the rat CD48 mutant T92A expressed on Jurkat T cells. The addition of imidazole did not influence the average 2D affinity (1/K_d), and the spread in affinities within the cell population was low, K_d = 4.9 ± 0.9 molecules/μm² (mean ± SD), despite an order of magnitude spread in ligand accumulation because of differences in receptor density. It was also found that cell contact size increased both with ligand density and with the number of receptors per cell but that the contact size stayed approximately constant when lowering the ligand density, above a density of around 10 rat CD2 molecules/μm², after the contact first had formed, indicative of a heterogeneous process. In summary, this method not only allows for single-cell affinities to be measured, but it can also reduce measurement and analysis time and improve measurement accuracy. Because of the low spread in 2D K_d within the cell population, the analysis can further be restricted to the cells showing the strongest binding, paving the way for using this method to study weak binding events.     

Investigation of Early Plasma Evolution Induced by Ultrashort Laser Pulses

Early plasma is generated owing to high intensity laser irradiation of target and the subsequent target material ionization. Its dynamics plays a significant role in laser-material interaction, especially in the air environment^1-11.Early plasma evolution has been captured through pump-probe shadowgraphy^1-3 and interferometry^1,4-7. However, the studied time frames and applied laser parameter ranges are limited. For example, direct examinations of plasma front locations and electron number densities within a delay time of 100 picosecond (ps) with respect to the laser pulse peak are still very few, especially for the ultrashort pulse of a duration around 100 femtosecond (fs) and a low power density around 10¹⁴ W/cm². Early plasma generated under these conditions has only been captured recently with high temporal and spatial resolutions¹². The detailed setup strategy and procedures of this high precision measurement will be illustrated in this paper. The rationale of the measurement is optical pump-probe shadowgraphy: one ultrashort laser pulse is split to a pump pulse and a probe pulse, while the delay time between them can be adjusted by changing their beam path lengths. The pump pulse ablates the target and generates the early plasma, and the probe pulse propagates through the plasma region and detects the non-uniformity of electron number density. In addition, animations are generated using the calculated results from the simulation model of Ref. ¹² to illustrate the plasma formation and evolution with a very high resolution (0.04 ~ 1 ps).Both the experimental method and the simulation method can be applied to a broad range of time frames and laser parameters. These methods can be used to examine the early plasma generated not only from metals, but also from semiconductors and insulators.     

Specific features of microheterogeneous plasma produced by irradiation of a polymer aerogel target with an intense 500-ps-long laser pulse

The properties of microheterogeneous plasma produced by irradiation of a polymer aerogel target with an intense (10¹⁴ W/cm³) short (0.5 ps) 1.064-μm laser pulse were studied. It is found that, even at plasma densities exceeding the critical density, a small fraction of the incident laser radiation penetrates through the plasma in which the processes of density and temperature equalization still take place. The intensification (as compared to plasmas produced from denser foams and solid films) of transport processes in such plasma along and across the laser beam can be caused by the initial microheterogeneity of the solid target. The replacement of a small (10% by mass) part of the polymer with copper nanoparticles leads to a nearly twofold increase in the intensity of the plasma X-ray emission.     

Fusicoccin Binding to Its Plasma Membrane Receptor and the Activation of the Plasma Membrane H-ATPase: I. Characteristics and Intracellular Localization of the Fusicoccin Receptor in Microsomes from Radish Seedlings

The characteristics of fusicoccin binding were investigated in microsomes from 24-h-old radish (Raphanus sativus L.) seedlings. The time course of fusicoccin binding depended on fusicoccin concentration: equilibrium was reached much faster at 10 nanomolar fusicoccin than at 0.3 nanomolar fusicoccin. Scatchard analysis of equilibrium binding as a function of fusicoccin concentration indicated a single class of receptor sites with a K_d of 1.8 nanomolar and a site density of 6.3 picomoles per milligram protein. Similar values (K_d 1.7 nanomolar and site density 7 picomoles per milligram protein) were obtained from the analysis of the dependence of equilibrium binding on membrane concentration at fixed fusicoccin concentrations. Fusicoccin binding comigrated with the plasma membrane H⁺-ATPase in an equilibrium sucrose density gradient: both activities formed a sharp peak (1.18 grams per milliliter) clearly distinct from that of markers of other membranes which all peaked at lower densities. The saturation profiles of fusicoccin binding and of fusicoccin-induced activation of the plasma membrane H⁺-ATPase, measured under identical conditions, were similar, supporting the view that fusicoccin-induced activation of the plasma membrane H⁺-ATPase is mediated by fusicoccin binding to its plasma membrane receptor.     

Laser Plasma Jet Driven Microparticles for DNA/Drug Delivery

This paper describes a microparticle delivery device that generates a plasma jet through laser ablation of a thin metal foil and uses the jet to accomplish particle delivery into soft living targets for transferring biological agents. Pure gold microparticles of 1 µm size were coated with a plasmid DNA, pIG121Hm, and were deposited as a thin layer on one surface of an aluminum foil. The laser (Nd:YAG, 1064 nm wavelength) ablation of the foil generated a plasma jet that carried the DNA coated particles into the living onion cells. The particles could effectively penetrate the target cells and disseminate the DNA, effecting the transfection of the cells. Generation of the plasma jet on laser ablation of the foil and its role as a carrier of microparticles was visualized using a high-speed video camera, Shimadzu HPV-1, at a frame rate of 500 kfps (2 µs interframe interval) in a shadowgraph optical set-up. The particle speed could be measured from the visualized images, which was about 770 m/s initially, increased to a magnitude of 1320 m/s, and after a quasi-steady state over a distance of 10 mm with an average magnitude of 1100 m/s, started declining, which typically is the trend of a high-speed, pulsed, compressible jet. Aluminum launch pad (for the particles) was used in the present study to make the procedure cost-effective, whereas the guided, biocompatible launch pads made of gold, silver or titanium can be used in the device during the actual clinical operations. The particle delivery device has a potential to have a miniature form and can be an effective, hand-held drug/DNA delivery device for biological applications.     

Study of a cumulative jet in a plasma focus discharge by the method of shearing interferometry

The dynamics of the cumulative jet formed in the course of plasma compression in a plasma focus discharge is investigated by the method of differential optical interferometry. The jet propagation velocity is found to be V = (2.3?C3) × 10⁷ cm/s, which coincides with the results of calculations performed in a 2D ideal MHD model. Ejection of matter from the anode in the late stage of the discharge due to the interaction of the cumulative jet and the electron beam with the anode surface is observed.     

Generation and interaction of intense counterpropagating plasma flows

Results are presented from experimental studies of the parameters of two counterpropagating (colliding) plasma flows generated by discharges in crossed electric and magnetic fields. It is shown that the conversion efficiency of the energy deposited in the discharges into the energy of directed plasma flows is 0.3–0.6. For discharge current pulses with a duration of ∼10 μs, the energy flux density in the plasma flow reaches ∼10 J/cm² and the total energy of the flow is on the order of 300 J. The density of deuterons in the flows is ∼10¹⁵ cm⁻³, and the flow velocity is ≤2×10⁷ cm/s. The total number of particles carried by the flows is about 10¹⁹. The possibility of using counterpropagating plasma flows to study reactions involving light nuclei (dd, pd, dt, and dHe reactions) in the range of ultralow collision energies is discussed. __________ Translated from Fizika Plazmy, Vol. 29, No. 8, 2003, pp. 714–721. Original Russian Text Copyright ? 2003 by Dudkin, Nechaev, Padalko, Bystritsky, Stolupin, Bystritskii, Voznyak.     

Processing and characterization of laser sintered hydroxyapatite scaffold for tissue engineering

The sintering processing of hydroxyapatite (HAP) powder was studied using selective laser sintering for bone tissue engineering. The effect of laser energy density on the microstructure, phase composition and mechanical properties of the sintered samples was investigated. The results indicate that the average grain size increases from 0.211 ± 0.039 to 0.979 ± 0.133 μm with increasing the laser energy density from 2.0 to 5.0 J/mm². The maximum value of Vickers hardness and fracture toughness were 4.0 ± 0.13 Gpa and 1.28 ± 0.033 MPam^1/2, respectively, when the laser energy density was 4.0 J/mm². The XRD results indicated that the nano-HAP was decomposed into TCP with the laser energy density of above 4.0 J/mm². In vitro bioactivity after soaking in simulated body fluid (SBF) for 3 ～ 12 days showed that a bone-like apatite layer on the surface of the sintered samples. It indicated that the HAP scaffold possesses favorable mechanical properties and bioactivity, and may be used for bone tissue engineering.     

Optical characteristics of a gallium laser plasma

Results are presented from studies of the emission from an erosion gallium laser plasma at a moderate intensity (W=(1–5)×10⁸ W/cm²) of a 1.06-μm laser radiation. It is shown that, under these conditions, the lower excited states of gallium atoms are populated most efficiently. Among the ions, only the most intense GaII lines are observed in the emission spectrum. The populations of GaI and GaII excited states are not related to direct electron excitation, but are determined by the recombination of gallium ions with slow electrons. The recombination times of GaIII and GaII ions in the core of the plasma jet are determined from the waveforms of emission in the GaII and GaI spectral lines and are equal to 10 and 140 ns, respectively. The results obtained are of interest for spectroscopic diagnostics of an erosion plasma produced from gallium-containing layered crystals during the laser deposition of thin films. __________ Translated from Fizika Plazmy, Vol. 27, No. 1, 2001, pp. 85–88. Original Russian Text Copyright ? 2001 by Shuaibov, Shimon, Dashchenko, Shevera, Chuchman.     

Investigation of the angular distribution and energy spectrum of fast ions produced during irradiation of targets by short laser pulses in the SOKOL-P facility

Results are presented from experimental investigations of the angular distributions and energy spectra of fast ions produced in deuterium polyethylene targets under irradiation by picosecond laser pulses with intensities of up to 2 × 10¹⁸ W/cm² in the SOKOL-P facility. The parameters of ion fluxes were measured by time-of-flight spectrometers based on semiconductor detectors.     

High-energy ion generation by short laser pulses

This paper reviews the many recent advances at the Center for Ultrafast Optical Science (CUOS) at the University of Michigan in multi-MeV ion beam generation from the interaction of short laser pulses focused onto thin foil targets at intensities ranging from 10¹⁷ to 10¹⁹ W/cm². Ion beam characteristics were studied by changing the laser intensity, laser wavelength, target material, and by depositing a well-absorbed coating. We manipulated the proton beam divergence using shaped targets and observed nuclear transformation induced by high-energy protons and deuterons. Qualitative theoretical approaches and fully relativistic two-dimensional particle-in-cell simulations modeled energetic ion generation. Comparison with experiments sheds light on ion energy spectra for multi-species plasma, the dependences of ion-energy on preplasma scale length and solid density plasma thickness, and laser-triggered isotope yield. Theoretical predictions are also made with the aim of studying ion generation for high-power lasers with the energies expected in the near future, and for the relativistic intensity table-top laser, a prototype of which is already in operation at CUOS in the limits of several-cycle pulse duration and a single-wavelength spot size.     

Laser-Driven Precipitation and Modification of Silver Nanoparticles in Soda Lime Glass Matrix Monitored by On-line Extinction Measurements

In this work, we investigated the effect of nanosecond laser irradiation at 532?nm on precipitation of Ag nanoparticles (NPs) in soda lime glasses doped with silver in the Ag^?+??CNa^?+? ion-exchange process. Formation and subsequent modification of Ag NPs during laser irradiation were studied by on-line extinction measurements making use of the localized surface plasmon resonance (LSPR). These investigations were further completed using scanning and transmission electron microscopies to examine the average size and distribution of nanoparticles within the sample. It has been shown that formation of NPs, its kinetics and the particle size strongly depend on the fluence and the total number of deposited laser pulses. It has been found that Ag NPs form after some specific number of pulses and they rapidly grow in size and number until some maximal value of extinction has been reached. Further irradiation of such samples only results in destruction of precipitated NPs due to photo-breakup, laser ablation confirmed by strong plasma emission observation. Moreover, due to strong irradiation, the host matrix can also be affected by changing its refractive index which manifests as the blue shift of the LSPR.     

A Study of High-Altitude Hypoxia-Induced Cell Stress in Murine Model

We evaluated the effects of high-altitude hypoxic stress in the murine model. For this purpose, 36 CR-mice in group A were maintained at the altitude of 3,820?m for hypoxia-induced factor (HIF)-1?? expression analysis by immunohistochemistry. The 36 Wistar rats in group B were maintained in low-pressure (400?C420?kPa) oxygen chamber, and the effects of hypoxia on myocardial mitochondria were studied. In the 36 CR-mice of group C, plasma vascular endothelial growth factor (VEGF) levels were determined using strept?Cavidin?Cbiotin complex/diaminobenzidine method after exposure to different altitudes/O₂-concentrations. The data show that in experimental group A1, endothelin (ET)-1?? concentrations gradually increased whereas HIF-1?? expression in myocardial cells was higher (P?<?0.01) than in control group A2. In rats of group B, the myocardial mitochondria numbers were reduced during the initial phase of acute stress response to hypoxia and cellular injury but, later, mitochondrial numbers were restored to normal values. In mice of experimental group C1, plasma VEGF concentrations increased under hypoxia, which were significantly higher (P?<?0.01) than those of control group C2. We, therefore, concluded that high-altitude hypoxia: (i) induced HIF-1?? expression; (ii) prompted adaptation/acclimatization after initial stress and cellular injury; and (iii) enhanced VEGF expression in murine.     

Estimation of the in situ degradation of the washout fraction of starch by using a modified in situ protocol and in vitro measurements

The in situ degradation of the washout fraction of starch in six feed ingredients (i.e. barley, faba beans, maize, oats, peas and wheat) was studied by using a modified in situ protocol and in vitro measurements. In comparison with the washing machine method, the modified protocol comprises a milder rinsing method to reduce particulate loss during rinsing. The modified method markedly reduced the average washout fraction of starch in these products from 0.333 to 0.042 g/g. Applying the modified rinsing method, the fractional degradation rate (k _d) of starch in barley, oats and wheat decreased from on average 0.327 to 0.144 h⁻¹ whereas for faba beans, peas and maize no differences in k _d were observed compared with the traditional washing machine rinsing. For barley, maize and wheat, the difference in non-fermented starch in the residue between both rinsing methods during the first 4 h of incubation increased, which indicates secondary particle loss. The average effective degradation of starch decreased from 0.761 to 0.572 g/g when using the new rinsing method and to 0.494 g/g when applying a correction for particulate matter loss during incubation. The in vitro k _d of starch in the non-washout fraction did not differ from that in the total product. The calculated ratio between the k _d of starch in the washout and non-washout fraction was on average 1.59 and varied between 0.96 for oats and 2.39 for maize. The fractional rate of gas production was significantly different between the total product and the non-washout fraction. For all products, except oats, this rate of gas production was larger for the total product compared with the non-washout fraction whereas for oats the opposite was observed. The rate of increase in gas production was, especially for grains, strongly correlated with the in vitro k _d of starch. The results of the present study do not support the assumption used in several feed evaluation systems that the degradation of the washout fraction of starch in the rumen is much faster than that of the non-washout fraction.