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Almeida AM Santos M Villalobos E Araújo SS van Dijck P Leyman B Cardoso LA Santos D Fevereiro PS Torné JM 《Protoplasma》2007,230(1-2):41-49
Summary. Following the establishment of a transgenic line of tobacco (B5H) expressing the trehalose-6-phosphate synthase (TPS) gene
from Arabidopsis thaliana, a preliminary immunolocalization study was conducted using leaves of adequately watered B5H and wild-type plants. Immunocytochemical
staining, followed by electron microscopy showed that the enzyme could be detected in both B5H and wild-type plants at two
different levels. Quantification showed the signal to be two to three times higher in transgenic plants than in the wild type.
This enzyme was markedly present in the vacuoles and the cell wall, and to a lesser extent in the cytosol. Moreover, a high
profusion of gold particles was detected in adjacent cells and in the sieve elements. Occasional spots were also detected
in chloroplasts and the nucleus, especially in the transgenic B5H line. No labeling signal was detected in mitochondria. Protein
localization seems to confirm the important role of TPS in sugar metabolism and transport through the plant, which could explain
its role in plant stress tolerance. Finally, it can be expected that TPS from tobacco has a relatively high similarity to
the TPS of Arabidopsis thaliana.
Correspondence and reprints: Laboratório de Biotecnologia de Células Vegetais, ITQB, Apartado 127, Avenida da República (E.A.N.),
2781-901 Oeiras, Portugal. 相似文献