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TFIIB and the regulation of transcription by RNA polymerase II 总被引:2,自引:0,他引:2
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The role of Zn(II) in transcription by T7 RNA polymerase 总被引:4,自引:0,他引:4
J E Coleman 《Biochemical and biophysical research communications》1974,60(2):641-648
Homogeneous T7 RNA polymerase contains from 2–4 gm atoms of zinc per mole of M.W. 107,000. Inactivated molecules which can be separated from the active molecules by repeated chromatography contain less zinc, from 0.4 to 1 gm at per mole. Instability of the enzyme makes it difficult to relate maximal activity to a specific stoichiometry of Zn. The enzyme is inhibited by 1,10-phenanthroline, EDTA, CN?, SH?, N3? and by incubation with Chelex resin. Zinc is retained on gel filtration, but can be removed by dialysis for 96 hr against 5 mM 1,10-phenanthroline which totally inactivates the enzyme. Catalytic activity requires the presence of thiol reagents. Preparations with low activity can be activated by exogenous Zn ions. 相似文献
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Exon tethering in transcription by RNA polymerase II 总被引:1,自引:0,他引:1
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