Spatially Oscillating Activity and Microbial Succession of Mercury-Reducing Biofilms in a Technical-Scale Bioremediation System

Mercury-contaminated chemical wastewater of a mercury cell chloralkali plant was cleaned on site by a technical-scale bioremediation system. Microbial mercury reduction of soluble Hg(II) to precipitating Hg(0) decreased the mercury load of the wastewater during its flow through the bioremediation system by up to 99%. The system consisted of a packed-bed bioreactor, where most of the wastewater's mercury load was retained, and an activated carbon filter, where residual mercury was removed from the bioreactor effluent by both physical adsorption and biological reduction. In response to the oscillation of the mercury concentration in the bioreactor inflow, the zone of maximum mercury reduction oscillated regularly between the lower and the upper bioreactor horizons or the carbon filter. At low mercury concentrations, maximum mercury reduction occurred near the inflow at the bottom of the bioreactor. At high concentrations, the zone of maximum activity moved to the upper horizons. The composition of the bioreactor and carbon filter biofilms was investigated by 16S-23S ribosomal DNA intergenic spacer polymorphism analysis. Analysis of spatial biofilm variation showed an increasing microbial diversity along a gradient of decreasing mercury concentrations. Temporal analysis of the bioreactor community revealed a stable abundance of two prevalent strains and a succession of several invading mercury-resistant strains which was driven by the selection pressure of high mercury concentrations. In the activated carbon filter, a lower selection pressure permitted a steady increase in diversity during 240 days of operation and the establishment of one mercury-sensitive invader.     

Spatially oscillating activity and microbial succession of mercury-reducing biofilms in a technical-scale bioremediation system

Mercury-contaminated chemical wastewater of a mercury cell chloralkali plant was cleaned on site by a technical-scale bioremediation system. Microbial mercury reduction of soluble Hg(II) to precipitating Hg(0) decreased the mercury load of the wastewater during its flow through the bioremediation system by up to 99%. The system consisted of a packed-bed bioreactor, where most of the wastewater's mercury load was retained, and an activated carbon filter, where residual mercury was removed from the bioreactor effluent by both physical adsorption and biological reduction. In response to the oscillation of the mercury concentration in the bioreactor inflow, the zone of maximum mercury reduction oscillated regularly between the lower and the upper bioreactor horizons or the carbon filter. At low mercury concentrations, maximum mercury reduction occurred near the inflow at the bottom of the bioreactor. At high concentrations, the zone of maximum activity moved to the upper horizons. The composition of the bioreactor and carbon filter biofilms was investigated by 16S-23S ribosomal DNA intergenic spacer polymorphism analysis. Analysis of spatial biofilm variation showed an increasing microbial diversity along a gradient of decreasing mercury concentrations. Temporal analysis of the bioreactor community revealed a stable abundance of two prevalent strains and a succession of several invading mercury-resistant strains which was driven by the selection pressure of high mercury concentrations. In the activated carbon filter, a lower selection pressure permitted a steady increase in diversity during 240 days of operation and the establishment of one mercury-sensitive invader.     

Root-zone constraints and plant-based solutions for dryland salinity

Limitations to agricultural productivity imposed by the root-zone constraints in Australian dryland soils are severe and need redemption to improve the yields of grain crops and thereby meet world demand. Physical, chemical and biological constraints in soil horizons impose a stress on the plant and restrict plant growth and development. Hardsetting, crusting, compaction, salinity, sodicity, acidity, alkalinity, nutrient deficiencies and toxicities due to boron, carbonates and aluminium are the major factors that cause these constraints. Further, subsoils in agricultural regions in Australia have very low organic matter and biological activity. Dryland salinity is currently given wide attention in the public debate and government policies in Australia, but they only focus on salinity induced by shallow groundwater. However, the occurrence of transient salinity in root-zone layers in the regions where water tables are deep is an important issue with potential for larger economic loss than water table-induced seepage salinity. Root-zone constraints pose a challenge for salinity mitigation in recharge as well as discharge zones. In recharge zones, reduced water movement in sodic horizons results in salt accumulation in the root zone resulting in chemical and physical constraints that reduce transpiration that, in turn, upsets salt balance and plant growth. High salinity in soil and groundwater restricts the ability of plants to reduce water table in discharge zones. Thus plant-based strategies must address different kinds of limitations in soil profiles, both in recharge and discharge zones. In this paper we give an overview of plant response to root-zone constraints but with an emphasis on the processes of salt accumulation in the root-zone of soils. We also examine physical and chemical methods to overcome subsoil limitations, the ability of plants to adapt to and ameliorate these constraints, soil modification by management of agricultural and forestry ecosystems, the use of biological activity, and plant breeding for resistance to the soil constraints. We emphasise that soil scientists in cooperation with agronomists and plant breeders should design site-specific strategies to overcome multiple soil constraints, with vertical and lateral variations, and to develop plant-based solutions for dryland salinity.     

Soil reconstruction after mining fails to restore soil function in an Australian arid woodland

The biogeochemical properties of soils drive ecosystem function and vegetation dynamics, and hence soil restoration after mining should aim to reinstate the soil properties and hydrological dynamics of remnant ecosystems. The aim of this study is to assess soil structure in two vegetation types in an arid ecosystem, and to understand how these soil properties compare to a reconstructed soil profile after mining. In an arid ecosystem in southeast Australia, soil samples were collected at five depths (to 105 cm) from remnant woodland and shrubland sites, and sites either disturbed or totally reconstructed after mining. We assessed soil physico‐chemical properties and microbial activity. Soils in the remnant arid ecosystem had coarse‐textured topsoils that overlay clay horizons, which allows water to infiltrate and avoid evaporation, but also slows drainage to deeper horizons. Conversely, reconstructed soils had high sand content at subsoil horizons and high bulk density and compaction at surface layers (0–20 cm). Reconstructed soils had topsoils with higher pH and electrical conductivity. The reconstructed soils did not show increased microbial activity with time since restoration. Overall, the reconstructed soil horizons were not organized in a way that allowed rainfall infiltration and water storage, as is imperative to arid‐zone ecosystem function. Future restoration efforts in arid ecosystems should focus on increasing sand content of soils near the surface, to reduce evaporative water loss and improve soil quality and plant health.     

Mercury-biogeochemical exploration for mineral deposits

Biogeochemical prospecting for mercury deposits and deposits of other minerals by the chemical analysis of mercury in plants or plant tissue that accumulate this element in linear (or near linear) proportion to the concentration in the soil is an effective method of exploration, even where allochthonous material as much as 200 to 2000 m thick covers the deposits. Plant tissues with this tendency to accumulate mercury (designated as non-barrier to mercury) comprise only a small fraction of the total of 255 types of plant tissues that were tested. Ten of these were considered to be quantitatively informative, and their mercury concentrations exceeded background values 300 or more times. The remaining types of plant tissues ranged in prospecting value from semi-quantitatively informative to qualitatively informative to uniformative (mercury values at or below background). The failure of some earlier uses of this prospecting method is attributed to the use of inappropriate plant tissues, to the mercury in the particular substrate studied existing in a form of low mobility and availability to plants, or to both causes.Prospecting by examining mercury concentrations in soils and rocks (lithogeochemical prospecting) is more effective than the biogeochemical approach only in prospecting for cinnabar deposits having no allochthonous cover. Mercury-biogeochemical prospecting is most effective for non-mercury mineral deposits and for oil and gas deposits. The types of plant tissues used in these studies are listed and are classified according to their value in prospecting. A case history is given of the Ozernoe pyrite-polymetallic deposit in Siberia.     

Phytoremediation of Mercury- and Methylmercury-Polluted Soils Using Genetically Engineered Plants

Inorganic mercury in contaminated soils and sediments is relatively immobile, though biological and chemical processes can transform it to more toxic and bioavailable methylmercury. Methylmercury is neurotoxic to vertebrates and is biomagnified in animal tissues as it is passed from prey to predator. Traditional remediation strategies for mercury contaminated soils are expensive and site-destructive. As an alternative we propose the use of transgenic aquatic, salt marsh, and upland plants to remove available inorganic mercury and methylmercury from contaminated soils and sediments. Plants engineered with a modified bacterial mercuric reductase gene, merA, are capable of converting Hg(II) taken up by roots to the much less toxic Hg(0), which is volatilized from the plant. Plants engineered to express the bacterial organo-mercurial lyase gene, merB, are capable of converting methylmercury taken up by plant roots into sulfhydryl-bound Hg(II). Plants expressing both genes are capable of converting ionic mercury and methylmercury to volatile Hg(0) which is released into an enormous global atmospheric Hg(0) pool. To assess the phytoremediation capability of plants containing the merA gene, a variety of assays were carried out with the model plants Arabidopsis thaliana, and tobacco (Nicotiana tabacum).     

Long‐term carbon storage through retention of dissolved aromatic acids by reactive particles in soil

Soils retain large quantities of carbon, thereby slowing its return to the atmosphere. The mechanisms governing organic carbon sequestration in soil remain poorly understood, yet are integral to understanding soil‐climate feedbacks. We evaluated the biochemistry of dissolved and solid organic carbon in potential source and sink horizons across a chronosequence of volcanic soils in Hawai'i. The soils are derived from similar basaltic parent material on gently sloping volcanic shield surfaces, support the same vegetation assemblage, and yet exhibit strong shifts in soil mineralogy and soil carbon content as a function of volcanic substrate age. Solid‐state¹³carbon nuclear magnetic resonance spectra indicate that the most persistent mineral‐bound carbon is comprised of partially oxidized aromatic compounds with strong chemical resemblance to dissolved organic matter derived from plant litter. A molecular mixing model indicates that protein, lipid, carbohydrate, and char content decreased whereas oxidized lignin and carboxyl/carbonyl content increased with increasing short‐range order mineral content. When solutions rich in dissolved organic matter were passed through Bw‐horizon mineral cores, aromatic compounds were preferentially sorbed with the greatest retention occurring in horizons containing the greatest amount of short‐range ordered minerals. These minerals are reactive metastable nanocrystals that are most common in volcanic soils, but exist in smaller amounts in nearly all major soil classes. Our results indicate that long‐term carbon storage in short‐range ordered minerals occurs via chemical retention with dissolved aromatic acids derived from plant litter and carried along preferential flow‐paths to deeper B horizons.     

Ecosystem wicks: Woodland trees enhance water infiltration in a fragmented agricultural landscape in eastern Australia

Abstract Since European settlement, Eucalyptus box woodlands have been substantially modified by agricultural practices, and in many areas in southern Australia are now restricted to scattered or clumped trees. We report here on a study to examine the impact of trees on water flow (infiltration) in an agricultural landscape with substantial areas of extant native vegetation. We examined infiltration through coarse‐ and fine‐textured soils within four landscape strata, the zones below Eucalyptus melliodora and Callitris glaucophylla canopies, the intertree zone dominated by perennial grasses and a landscape homogenized by cultivation and dominated by annual crops. We measured sorptivity, the early phase of water flow, and steady‐state infiltration with disc permeameters at two supply potentials. These different potentials enabled us to separate infiltration into (i) flow through large (biopores) and small pores and (ii) flow through small pores only where biopores are prevented from conducting water. On the fine‐textured soils, both sorptivity and steady‐state infiltration were significantly greater (approximately fivefold) under the timbered strata compared with the grassy slopes or cultivation. Differences were attributable to the greater proportion of macropores below the tree canopies compared with the nontimbered strata. The lack of a significant difference on the coarse‐textured soils, despite their macropore status, was attributed to differences in surface litter and plant cover, which would maintain continuous macropores at the surface and thus conduct large amounts of water. The tendency of slopes covered by cryptogamic crusts and grasses to shed run‐off and for the trees to absorb substantial quantities of water reinforced the important ecological service provided by trees, which moderates large run‐off events and captures small amounts of water leaking from the grassy patches. In the absence of these ‘ecosystem wicks’, run‐off would find its way into regional groundwater and contribute to rising salinity.     

Strategies for the engineered phytoremediation of toxic element pollution: mercury and arsenic

Plants have many natural properties that make them ideally suited to clean up polluted soil, water, and air, in a process called phytoremediation. We are in the early stages of testing genetic engineering-based phytoremediation strategies for elemental pollutants like mercury and arsenic using the model plant Arabidopsis. The long-term goal is to develop and test vigorous, field-adapted plant species that can prevent elemental pollutants from entering the food-chain by extracting them to aboveground tissues, where they can be managed. To achieve this goal for arsenic and mercury, and pave the way for the remediation of other challenging elemental pollutants like lead or radionucleides, research and development on native hyperaccumulators and engineered model plants needs to proceed in at least eight focus areas: (1) Plant tolerance to toxic elementals is essential if plant roots are to penetrate and extract pollutants efficiently from heterogeneous contaminated soils. Only the roots of mercury- and arsenic-tolerant plants efficiently contact substrates heavily contaminated with these elements. (2) Plants alter their rhizosphere by secreting various enzymes and small molecules, and by adjusting pH in order to enhance extraction of both essential nutrients and toxic elements. Acidification favors greater mobility and uptake of mercury and arsenic. (3) Short distance transport systems for nutrients in roots and root hairs requires numerous endogenous transporters. It is likely that root plasma membrane transporters for iron, copper, zinc, and phosphate take up ionic mercuric ions and arsenate. (4) The electrochemical state and chemical speciation of elemental pollutants can enhance their mobility from roots up to shoots. Initial data suggest that elemental and ionic mercury and the oxyanion arsenate will be the most mobile species of these two toxic elements. (5) The long-distance transport of nutrients requires efficient xylem loading in roots, movement through the xylem up to leaves, and efficient xylem unloading aboveground. These systems can be enhanced for the movement of arsenic and mercury. (6) Aboveground control over the electrochemical state and chemical speciation of elemental pollutants will maximize their storage in leaves, stems, and vascular tissues. Our research suggests ionic Hg(II) and arsenite will be the best chemical species to trap aboveground. (7) Chemical sinks can increase the storage capacity for essential nutrients like iron, zinc, copper, sulfate, and phosphate. Organic acids and thiol-rich chelators are among the important chemical sinks that could trap maximal levels of mercury and arsenic aboveground. (8) Physical sinks such as subcellular vacuoles, epidermal trichome cells, and dead vascular elements have shown the evolutionary capacity to store large quantities of a few toxic pollutants aboveground in various native hyperaccumulators. Specific plant transporters may already recognize gluthione conjugates of Hg(II) or arsenite and pump them into vacuole.     

Some microbiological aspects of surface and buried soils on Mt. Kenya,East Africa

When numbers of microorganisms in profiles of surface and buried horizons on Mt. Kenya were estimated by dilution plate counting they were found to be consistently lower than those from other soils in different geographical regions as determined from the literature. The lower numbers are probably characteristic of the poorly weathered Inceptisols and Entisols usually found in the alpine zone.The A horizons of the soils studied contain proportionately fewer of the total numbers of organisms in the A, B and C horizons than observed in most soils. Estimates of organic matter were positively correlated with numbers of fungi and bacteria in the A horizons. However, other factors such as severe drought, high light intensity, low temperatures, diurnal frost heaving, low pH and paucity of clay minerals may be significant factors in suppressing the more luxuriant growth of microbial populations.Organic and inorganic horizons of buried soils sometimes exhibit higher counts of microorganisms than adjacent horizons of surface soils. However, the bacteria and fungi even in deeply buried paleosols exhibit characteristics of an unspecialized heterotrophic population. Among fungi the species were obviously the same as those isolated from one or more of the overlying horizons. Taken in conjunction with other evidence from the profiles it is concluded that the microorganisms were introduced and represent a transient or non-active population. Contamination of buried organic horizons may influence the estimated age as assessed by radiocarbon dating.     

Strategies for the engineered phytoremediation of toxic element pollution: mercury and arsenic

Plants have many natural properties that make them ideally suited to clean up polluted soil, water, and air, in a process called phytoremediation. We are in the early stages of testing genetic engineering-based phytoremediation strategies for elemental pollutants like mercury and arsenic using the model plant Arabidopsis. The long-term goal is to develop and test vigorous, field-adapted plant species that can prevent elemental pollutants from entering the food-chain by extracting them to aboveground tissues, where they can be managed. To achieve this goal for arsenic and mercury, and pave the way for the remediation of other challenging elemental pollutants like lead or radionucleides, research and development on native hyperaccumulators and engineered model plants needs to proceed in at least eight focus areas: (1) Plant tolerance to toxic elementals is essential if plant roots are to penetrate and extract pollutants efficiently from heterogeneous contaminated soils. Only the roots of mercury- and arsenic-tolerant plants efficiently contact substrates heavily contaminated with these elements. (2) Plants alter their rhizosphere by secreting various enzymes and small molecules, and by adjusting pH in order to enhance extraction of both essential nutrients and toxic elements. Acidification favors greater mobility and uptake of mercury and arsenic. (3) Short distance transport systems for nutrients in roots and root hairs requires numerous endogenous transporters. It is likely that root plasma membrane transporters for iron, copper, zinc, and phosphate take up ionic mercuric ions and arsenate. (4) The electrochemical state and chemical speciation of elemental pollutants can enhance their mobility from roots up to shoots. Initial data suggest that elemental and ionic mercury and the oxyanion arsenate will be the most mobile species of these two toxic elements. (5) The long-distance transport of nutrients requires efficient xylem loading in roots, movement through the xylem up to leaves, and efficient xylem unloading aboveground. These systems can be enhanced for the movement of arsenic and mercury. (6) Aboveground control over the electrochemical state and chemical speciation of elemental pollutants will maximize their storage in leaves, stems, and vascular tissues. Our research suggests ionic Hg(II) and arsenite will be the best chemical species to trap aboveground. (7) Chemical sinks can increase the storage capacity for essential nutrients like iron, zinc, copper, sulfate, and phosphate. Organic acids and thiol-rich chelators are among the important chemical sinks that could trap maximal levels of mercury and arsenic aboveground. (8) Physical sinks such as subcellular vacuoles, epidermal trichome cells, and dead vascular elements have shown the evolutionary capacity to store large quantities of a few toxic pollutants aboveground in various native hyperaccumulators. Specific plant transporters may already recognize gluthione conjugates of Hg(II) or arsenite and pump them into vacuole.

     

天津汉沽农田土壤－植物系统中汞的分布、迁移和积累

本文研究了受汞污染的农田土壤—植物系统中汞的分布,迁移和积累的规律。土壤中的汞在离污染源3公里的范围内含量最高;主要集中在0一20厘米的土壤上层,几乎不往下迁移。植物可以从土壤和大气中吸收、积累汞。在汉沽区没有发现由于汞污染所造成的植物受害症状。植物中的汞含量与土壤中的汞含量成正相关。土壤汞含量与水稻茎叶汞含量的相关系数为0.836(N=7),与糙米汞含量的相关系数为0.898(N=7)。植物不同部位的汞含量根>叶>茎>种子。不同作物种子比较,糙米>高粱>小麦。在大气中汞含量高的地段,植物地上部分汞含量高于根。土壤、植物中的汞不断地向大气扩散,而大气中的汞随着降雨、降尘等又不断地沉降到土壤和植物的气生表面,并可被植物吸收。汞向其邻近地区扩散的能力较小。     

Potential of S-containing and P-containing complexones in improving phytoextraction of mercury by Trifolium repens L.

Mercury is a global pollutant in the modern world. There is a large number of areas in the world where mercury is present in soils in significant quantities. Remediation methods which have traditionally been proposed may pose a risk of secondary mercury contamination and/or adverse health effects for cleaners. Phytoextraction of heavy metals from the soil environment is currently considered one of the promising non-invasive methods of remediation. But this approach has limited effectiveness. Chemically induced phytoextraction can increase the efficiency of this process both by converting less bioavailable mercury compounds to bioavailable fractions in the soil and by increasing the rate of transfer of metals in plants. This paper presents the results of a screening study of various chemical amendments to enhance the phytoextraction of mercury by Trifolium repens L. The results showed good potential for the induction of phytoextraction of phosphorus(P) and sulfur (S)-containing chelates. With this study, for the first time for the phytoextraction of mercury, the monoethanolamine salt of 2,2′-(ethylenedithio) diacetic acid was used as the S-containing chelate, and the disubstituted potassium salt of 1-hydroxy ethylidene-1,1-diphosphonic acid was used as the P-containing chelate. Further attention is given to study the effect that exogenous application of phytohormones and plant growth regulators has on the efficiency of mercury absorption and physiological status of plants, which performed well in combination with a P-containing chelate.     

Sulfur in Ghanaian soils

Studies were carried out to examine factors which might influence the distribution of S in Ghanaian soils. Nine soil profiles developed over granitic rocks, three each representing the upper slope (US), middle slope (MS) and lower slope (LS) of catena in the evergreen high rain forest (ERF), semi-deciduous rain forest (SDF) and the interior savanna (ISAV) zone of Ghana were selected. The total S contents varied from 9 to 347 ppm; the average for all the surface and subsurface horizons was 141 ppm and for subsoils 105 ppm. The contents also varied according to: (1) the ecological zone as follows: ERF 0) SDF>ISAV and (2) the topographic position: US>MS>LS. The total S was closely correlated with organic C and total N in the surface and subsurface horizons (r=0.931^*** and 0.941^*** respectively). Inorganic sulfate was generally higher in the subsoils than in the surface and subsurface horizons of the ERF and SDF profiles whereas the opposite was the case in the ISAV profiles. Based on the critical value of 6 ppm in surface soils, all the savanna soils would be considered S deficient.The total organic S, which constituted from 56 to over 95% of the total S in the profiles, was significantly correlated with total N both in the surface and subsurface horizons (N:S ratio=9.1:1) and in the subsoils (N:S ratio=7.6:1). Fractionation of the organic S showed that HI-reducible S ranged from 14 to 117 ppm in the surface and subsurface horizons (average 55 ppm, equivalent to 47% of the total organic S) and from 2 to 169 ppm (average 55 ppm, equivalent to 60% of the total organic S) in the subsoils. The C-bonded S ranged from 6 to 223 ppm (average 73 ppm, equivalent to 57% of the total organic S) in the surface and subsurface horizons and from 1 to 83 ppm (average 29 ppm, equivalent to 32% of the total organic S) in the subsoils. HI-reducible S was significantly correlated with organic C (r=0.805^***) and total N (r=0.845^***) in the surface and subsurface horizons only whereas C-bonded S was significantly correlated with organic C and total N in both the surface and subsurface horizons and subsoils (r=0.870^*** and 0.624^*** respectively).The N:S ratios varied from 6.0 to 12.7 in the surface and subsurface horizons and from 0.5 to 27.3 in the subsoils. However the N:S ratio was less variable within the profile than the C:S ratio. The C:N:S ratios varied considerably within the profile and among the different soils but they fall within the range of values reported world-wide.     

Growth and mineral nutrition of plant species from clearings on different horizons of an iron-humus podzol profile

Summary Four plant species of woodland clearings — Avenella flexuosa, Chamaenerion angustifolium, Rumex acetosella, and Senecio sylvaticus — were grown on five different horizons of an iron-humus podzol profile. Reasonable growth was achieved only on the A₀ horizon, which was rich in organic matter. The growth reduction on the other horizons was correlated with low concentrations of manganese and phosphorus in soils and plant organs. The restriction of so-called acidophilous species to acid soils is interpreted as meaning that they have a need for a high supply of manganese.     

Plant species richness and diversity of the serpentine areas on the Witwatersrand

Soil chemistry can play an important role in determining plant diversity. Serpentine soils are usually toxic to many plant taxa, which limits plant diversity compared to that on adjacent non-serpentine soils. The usually high concentrations of toxic metals in serpentine soils are considered to be the edaphic factors that cause low diversity and high endemism. This paper aimed primarily to determine whether there is a relationship between serpentine soil chemistry and species richness on the Witwatersrand and to compare species richness of the serpentine areas with that of adjacent non-serpentine areas as well as with the species richness of the serpentine areas in the Barberton Greenstone Belt. The alpha- and beta-diversity of the Witwatersrand serpentine and non-serpentine areas was also investigated. A secondary aim of this study was to determine which of the non-serpentine taxa were more common on the serpentine than off the serpentine, which taxa were more common off the serpentine than on the serpentine and which taxa were equally common on and off serpentine soils. There was no significant difference in alpha-diversity between the serpentine and the adjacent non-serpentine areas, but beta-diversity is higher between serpentine plots than between non-serpentine plots. Although soil factors do affect species richness and diversity of plants on the Witwatersrand to a limited extent, the concentrations of soil chemicals in serpentine soils are not sufficiently different from those in non-serpentine soils to significantly influence the species richness and diversity of the serpentine soils. The high, but similar, diversity on serpentine and non-serpentine soils on the Witwatersrand indicates that soil factors do not play a significant role in determining diversity on potentially toxic soils in the area.     

Distribution and mobility of lead in soils at an outdoor shooting range

The distribution of total Pb in surface and subsurface soil horizons at an outdoor shooting range in southeastern Michigan was determined by single extraction elemental analysis (AAS and ICP‐AES). Significant Pb enrichment of the site's soils coincides closely with Pb vapor and particulate matter produced from shot shell primers and the downfall of Pb/Sb pellets associated with the recreational shooting of skeet and trap. Surface concentrations in these locations are 10 to 100 times greater than the background concentration found on adjacent properties. The distribution of Pb in the subsurface soil horizons corresponds to the distribution of Pb at the surface, which suggests the Pb is mobilizing and migrating downward through the vadose zone. This mobilization appears to be occurring despite the clay‐rich nature of the soils, and may be due to the transformation of metallic Pb into soluble Pb compounds of carbonate and sulfate: Both compounds appear to be present in crust material found coating many of the pellets found at the site. The downward migration of soluble Pb is a potential threat to groundwater that is present at the site at a depth of less than 1 m. The protection of surface water quality is also a concern because Pb pellets from the shooting range have been found in the bed sediments of a nearby stream.     

Predicting arsenic bioavailability to hyperaccumulator Pteris vittata in arsenic-contaminated soils

Using chemical extraction to evaluate plant arsenic availability in contaminated soils is important to estimate the time frame for site cleanup during phytoremediation. It is also of great value to assess As mobility in soil and its risk in environmental contamination. In this study, four conventional chemical extraction methods (water, ammonium sulfate, ammonium phosphate, and Mehlich III) and a new root-exudate based method were used to evaluate As extractability and to correlate it with As accumulation in P. vittata growing in five As-contaminated soils under greenhouse condition. The relationship between different soil properties, and As extractability and plant As accumulation was also investigated. Arsenic extractability was 4.6%, 7.0%, 18%, 21%, and 46% for water, ammonium sulfate, organic acids, ammonium phosphate, and Mehlich III, respectively. Root exudate (organic acids) solution was suitable for assessing As bioavailability (81%) in the soils while Mehlich III (31%) overestimated the amount of As taken up by plants. Soil organic matter, P and Mg concentrations were positively correlated to plant As accumulation whereas Ca concentration was negatively correlated. Further investigation is needed on the effect of Ca and Mg on As uptake by P. vittata. Moreover, additional As contaminated soils with different properties should be tested.     

Soil Organic Carbon Content Decreases in Both Surface and Subsoil Mineral Horizons by Simulated Future Increases in Labile Carbon Inputs in a Temperate Coniferous Forest

Soils represent important pools of soil organic carbon (SOC) that can be greatly influenced by labile C inputs, which are expected to increase in future due to CO₂ enrichment of atmosphere and a concomitant rise in plant primary productivity. Studying effects of variable labile C inputs on SOC pool helps to understand how soils respond to global change. However, this knowledge is missing for coniferous forest soils despite being widespread throughout the northern temperate zone. We conducted a 7-month field manipulation experiment to study the effects of variable labile C inputs (simulated by additions of C₄ sucrose) on the C content in soil fractions and on microbial abundance in the organic (O), surface mineral (A), and subsoil mineral (B) horizons of a temperate coniferous forest soil. SOC in less-protected soil fractions and total organic C were substantially decreased by labile C additions that simulated future increases in C inputs. The SOC losses were comparable between the A and B horizon (40% vs. 30%). However, because sucrose availability estimated from its incorporation into soil fractions and microbial biomass sharply decreased with soil depth, the loss of C was higher in the B than in the A horizon when related to the amount of sucrose added. Utilization of sucrose was highest by fungi in the O horizon and by bacteria in the mineral soil horizons. The results indicate that future increases in labile C inputs to coniferous forest soils will cause rapid and substantial losses of SOC in both the surface and subsoil mineral horizons.

     

Microbiological processes in the Severnyi deep disposal site for liquid radioactive wastes

Local monitoring of physicochemical, radiochemical, and microbiological parameters was performed in the deep horizons of the Severnyi site used for disposal of liquid radioactive waste (LRW). Analysis of the chemical and radiochemical composition of the wastes and formation fluid revealed that the boundary for migration of radionuclides lagged behind that for nonradioactive waste components (sodium nitrate) and tritium. The physicochemical and radiochemical conditions in deep horizons did not prevent microbial growth. The numbers of microorganisms (aerobic organotrophs, denitrifying, fermentative, sulfate-reducing, and methanogenic) were low, as were the rates of sulfate reduction and methanogenesis; they increased in the waste dispersion zone. The microorganisms from deep horizons were able to produce gases (CH₄, CO₂, N₂, and H₂S) from possible waste components. Denitrifying bacteria belonged to different Pseudomonas species and reduced nitrate to dinitrogen under the conditions of pH, salinity, temperature, and radioactivity found in the disposal site. These results suggest the need for control of microbiological processes in deep disposal site for liquid RW.