磁处理水对家兔血脂影响的实验研究

目的:探讨饮用磁处理水时间的长短与降低家兔血脂的关系。方法:72只家兔平均分四组A组基础饲料组,饮自来水;B、C、D高脂饲料组,B组对照组饮自来水;C组治疗1组,30天后饮磁处理水,治疗30天后采耳血,分别测血清TC、TG、HDL-c、LDL-c水平。D组治疗2组,30天后饮磁处理水,治疗100天后采耳血,分别测血清TC、TG、HDL-c、LDL-c水平。结果:B组家兔血清TC、TG、HDL-c、LDL-c水平显著高于A组,(P<0.01);C组家兔TC、LDL-c水平显著低与B组(P<0.01);,但也显著高于A组(P<0.01);TG、HDL-c水平与B组相比无显著差异(P>0.05)。D组家兔血清TC、TG、LDL-c水平与B组相比均有明显下降(P<0.01),与A组比较差异无显著性(P>0.05),而HDL-c水平与A组比较明显上升(P<0.01)。结论:长期饮用磁处理水可以显著降低家兔血清高胆固醇含量,并恢复到正常状态。     

西藏芫根提取物对小鼠抗缺氧作用的初步研究

实验研究西藏芫根提取物的抗缺氧活性.通过建立小鼠常压缺氧模型、急性脑缺血性缺氧模型以及亚硝酸钠中毒模型,以蒸馏水作为空白对照,盐酸普萘洛尔作为阳性对照,观察芫根水、65%乙醇及95%乙醇提取物的10 g/kg、15 g/kg和20 g/kg剂量组对小鼠缺氧的保护作用,同时观察各提取物10 g/kg剂量组对红细胞数量和血红蛋白含量的影响.结果表明,芫根水提取物、65%乙醇提取物及95%乙醇提取物均能明显延长模型小鼠在常压缺氧、急性脑缺血性缺氧及亚硝酸钠中毒时的存活时间,并可增加外周血象中红细胞数与血红蛋白含量,显示芫根提取物具有抗缺氧活性.     

磁处理水对家兔血脂影响的实验研究

目的：探讨饮用磁处理水时间的长短与降低家兔血脂的关系.方法：72只家兔平均分四组A组基础饲料组,饮自来水;B、C、D高脂饲料组,B组对照组饮自来水;C组治疗1组,30天后饮磁处理水,治疗30天后采耳血,分别测血清TC、TG、HDL-c、LDL-c水平.D组治疗2组,30天后饮磁处理水,治疗100天后采耳血,分别测血清TC、TG、HDL-c、LDL-c水平.结果：B组家兔血清TC、TG、HDL-c、LDL-c水平显著高于A组,（P〈0.01）;C组家兔TC、LDL-c水平显著低与B组（P〈0.01）;,但也显著高于A组（P〈0.01）;TG、HDL-c水平与B组相比无显著差异（P〉0.05）.D组家兔血清TC、TG、LDL-c水平与B组相比均有明显下降（P〈0.01）,与A组比较差异无显著性（P〉0.05）,而HDL-c水平与A组比较明显上升（P〈0.01）.结论：长期饮用磁处理水可以显著降低家兔血清高胆固醇含量,并恢复到正常状态.     

芫根总皂苷对饮食诱导肥胖大鼠减肥降脂机制研究

以高脂饮食诱导建立肥胖大鼠模型,研究芫根总皂苷减肥降脂的作用机制。以奥利司他(48 mg/kg)为阳性药,设芫根总皂苷3个剂量组(500 mg/kg、1000 mg/kg、1500 mg/kg),灌胃给药45 d。检测血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、脂蛋白脂酶(LPL)、肝脂酶(HL)、卵磷脂胆固醇酰基转移酶(LCAT)、胰脂肪酶(PL)的活性及丙二醛(MDA)、游离脂肪酸(FFA)、瘦素(LEP)的含量。结果显示与高脂模型组相比,芫根总皂苷可不同程度升高SOD、GSH-Px、LPL、HL活性,抑制PL活性,降低MDA、FFA、LEP含量,其可能的作用机制是提高抗氧化能力,降低肝脂质损伤,促进脂质分解,抑制脂肪吸收,改善脂质代谢及瘦素抵抗等。     

蝇蛆壳聚糖对实验性糖尿病大鼠脂代谢的影响

目的测定实验糖尿病大鼠血脂,探讨蝇蛆壳聚糖改善脂代谢以降低血糖的机制。方法用全自动生化分析仪测定各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)的含量。结果经蝇蛆壳聚糖治疗后,高剂量组TC,TG含量明显低于模型对照组(P<0.01),其中TG含量与拜糖平组相比有明显差异(P<0.05)。中剂量组TC含量明显低于模型对照组(P<0.01),TG含量低于模型对照组(P<0.05)。低剂量组HDL含量最高,与模型对照组相比有显著差异(P<0.01)。结论蝇蛆壳聚糖有改善实验糖尿病大鼠血脂的作用。     

屎肠球菌DM891129菌株对大鼠高血脂症的调节作用

对屎肠球菌DM 891129 菌株进行调节血脂作用的功能实验。实验动物采用二级w istar 雄性大鼠,饲以高胆固醇脂饲料再同时饲以本活菌制剂。14 和28 天后,分别测定大鼠血清胆固醇(TC)、血清甘油三脂(TG)和血清高密度脂蛋白(HDL- C)。实验结果显示:实验组中高中低剂量组血清胆固醇浓度比对照组低(P< 0.05);血清TG 浓度、血清HDL- C 浓度、体重实验组与对照组相比组内和组间差异不明显。结果表明:与饲以高脂饲料的对照组大鼠相比,实验组的大鼠血清TC 浓度明显低于对照组(P< 0.05)。实验结果证实屎肠球菌具有调节大鼠血脂的作用     

一次密度梯度超速离心分离人血清的VLDL、LDL、HDL_2、HDL_3及无脂血清

本文将密度梯度、离心力和离心时间作适当的组合和配比:即不连续密度梯度1.000—1,400g/mlNaBr溶液、离心力168,000g、22小时,10℃,在Beckman L8-80型、区带头Ti-14一次超速离心将血清中四种主要脂蛋白和无脂血清相互分开,获得五个明显的蛋白峰。前四个峰经琼脂糖电泳,聚丙烯酰胺电泳,免疫双扩散和分析超速离心鉴定分别为VLDL、LDL、HDL_2和HDL_3,不含清蛋白。峰五为无脂血清,仅含0.046mg/dl胆固醇和0.2mg/dl甘油三酯,本法重复性佳,分离样品多(50ml),效果好,操作简单,并可延长离心机头的使用期限。已用于研究各种因素对脂蛋白含量的影响及其代谢间的相互关系。     

嗜酸乳杆菌及其制剂对大鼠血脂代谢的影响

本实验表明,口服嗜酸乳杆茵及其制剂,能升高大鼠ADL—C和HDL—C/T比值及降低[VLDL LDL]—C的浓度。这一作用对于防止动粥发生显然是十分有利的。若将嗜酸乳杆菌及其制剂,与其它降血脂药物配伍,用于高血脂症的辅助治疗,将会产生良好的效果。     

新安软胶囊对大鼠脂肪肝动物模型的影响

目的:研究新安软胶囊对大鼠脂肪肝模型动物的影响.方法:建立大鼠脂肪肝动物模型后,以血清中生化指标(ALT、AST、LDL、HDL-C)测定、血清和组织中CHO、TG、MDA、SOD含量测定等为检测指标,比较新安软胶囊的量效与时效关系.结果:①每个新安软胶囊组与模型组及对照组比较均能明显降低ALT、AST、LDL、CHO、TG、MDA、SOD (P＜0.05),以及明显升高HDL-C(P＜0.05),其剂量效果是新安软胶囊62.5 mg/kg组＜125mg/kg组＜250 mg/kg组,其时间效果是用药后第2周＜第4周＜第6周;②新安软胶囊中、大剂量组与模型组比较有明显统计学差异(P＜0.05).结论:新安软胶囊对大鼠脂肪肝模型具有明显的治疗作用.     

牛磺胆酸钠在大鼠急性胰腺炎模型中的半数致死量(LD50)研究

目的:通过使用不同浓度的牛磺胆酸钠(TC)建立急性胰腺炎(AP)模型,观察相应的死亡率,进而计算其半数致死量。方法:使用不同浓度的TC(0%(wt/vl)、1%、2%、3%、4%、5%、9%)观察AP造模72小时后各组的死亡率。同时分析TC浓度与组织病理评分、血清淀粉酶和外周血细胞变化的相关性。结果:TC的半数致死量为3.409%。并且,TC的浓度与组织病理评分、血清淀粉酶和外周血细胞变化密切相关。结论:TC所致AP造模的最佳剂量为3.409%。     

翻白草对大鼠和家兔高血脂模型的降血脂作用

目的探讨翻白草对高血脂大鼠和家兔的降血脂作用。方法采用Wistar大鼠60只（其中50只为高脂饲料诱导成高血脂动物模型,10只为空白对照组）,家兔36只（其中30只为高脂饲料诱导成高血脂动物模型,6只为空白对照组）。随机分为6组（翻白草大、中、小3个剂量组,模型组,脂必妥对照组,空白对照组）。连续给药6周,分别于给药后第4周、第6周采血,检测血清中胆固醇（TC）、甘油三脂（TG）及低密度脂蛋白（LDL）浓度。结果翻白草各给药组与模型组比较,给药后第4周,翻白草可降低高血脂大鼠和家兔血清中TC、TG、LDL的含量（P〈0.05）;给药后第6周,可显著降低高血脂大鼠和家兔血清中TC、TG、LDL的含量（P〈0.01）。结论翻白草具有很好的降血脂作用。     

藏药玛宁江巴提取物对大鼠利尿作用的初步研究

目的:研究玛宁江巴系统溶剂提取物对大鼠的利尿作用.方法:采用清醒状态下正常wistar大鼠,观察玛宁江巴各系统溶剂提取物的利尿作用.结果:玛宁江巴石油醚提取物、乙酸乙酯提取物有显著促进排尿的作用.结论:玛宁江巴利尿作用的有效成分主要集中于石油醚提取物及乙酸乙酯提取物中,可能是脂肪酸及黄酮类成分.     

Impact of mating design on selection response in Brassica rapa L.

The impact of four mating designs on selection response for leaf area was assessed at four different population sizes, using fast-cycling Brassica rapa L. Mating designs were either balanced (partial diallel or pair mating) or unbalanced (factorial mating designs with either one or two testers). When balanced, the mating designs required different numbers of crossings for the same number of parents: the partial diallel design, in the configuration retained here, required three times as many crossings as pair mating. Population sizes were 4, 8, 16, and 32. The percentage of selected individuals was kept constant at 25%. Despite an average estimated heritability around 0.4, the overall response to selection after five generations was fairly weak in all three replicates. For a given population size, selection response was larger under balanced mating designs than under unbalanced ones. There was no difference among balanced mating designs. Both results indicate that effective population size is more important than population size or the number of crossings in maintaining genetic gain.     

Genetic diversity of allozymes in turnip (Brassica rapa L. var. rapa) from the Nordic area

Genetic diversity and relationships based on isozymes were studied in 31 accessions of turnip (Brassica rapa L. var. rapa). The material included varieties, elite stocks, landraces and older turnip of slash-and-burn type from the Nordic area. A total of 9 isozyme loci and 26 alleles were studied. The isozyme systems were ACO, DIA, GPI, GOT, PGM, PGD and SKD. The level of heterozygosity was reduced in the landraces, but it was high for the variety group 'Ostersundom'. Turnip has a higher genetic variation than other crops within B. rapa and than in other species with the same breeding system. The genetic diversity showed that 18.7% of the genetic variation was within the accessions, and the total H tau value was 0.358. Gpi-I and Pgd-I showed the lowest variation compared with the other loci. The cluster analysis revealed five clusters, with one main cluster including 25 of the 31 accessions. The dendrogram indicated that the variety group 'Ostersundom' clustered together whereas the variety group 'Bortfelder' was associated with country of origin. The landraces were spread in different clusters. The 'slash-and-burn' type of turnip belonged to two groups.     

苦瓜提取物的抑菌活性研究

本文研究了苦瓜(MC)不同活性成分的抑菌活性.结果表明:苦瓜提取物对细菌的抑菌作用明显,但对霉菌、酵母菌的抑制效果不明显.苦瓜粗皂甙和苦瓜粗多糖的抑菌效果比纯品的好.苦瓜乙醇浸提物、苦瓜粗多糖、苦瓜多糖、苦瓜粗皂甙、苦瓜皂甙、苦瓜粗蛋白对藤黄八叠球菌的最低抑菌浓度(MIC)分别为50、100、5、100、2、20 mg/mL.苦瓜多糖、苦瓜皂甙和苦瓜蛋白是苦瓜抑菌活性功能成分.     

蔗糖诱发Sprague—Dawley雄性大鼠肥胖病高脂血症

用蔗糖作辅助铁水,饲养刚离乳的Sprague－Dawley雄性大鼠,180天后,53％大鼠肥胖成为饮食诱发肥胖型大鼠（DIO大鼠）,47％大鼠不肥胖成为饮食诱发肥胖抵抗型大鼠（DIO－R大鼠）且DIO和DIO－R大鼠均具高脂血症。说明25％蔗糖可诱发Sprague-Dawley雄性大鼠肥胖病高脂血症,但同样条件下为什么部分大鼠不肥胖还有待于进一步研究。另外,对DIO大鼠的肥胖机制作了初步探讨。     

Genomic Markers Associated with Cold-Hardiness in Brassica rapa L.

Molecular Biology - Brassica rapa L. is a valuable and widespread species, but its cultivation in risk farming areas requires high-quality cold-hardy varieties to be developed. Mechanisms of the...     

Pollination and embryo development in Brassica rapa L. in microgravity

Plant reproduction under spaceflight conditions has been problematic in the past. In order to determine what aspect of reproductive development is affected by microgravity, we studied pollination and embryo development in Brassica rapa L. during 16 d in microgravity on the space shuttle (STS-87). Brassica is self-incompatible and requires mechanical transfer of pollen. Short-duration access to microgravity during parabolic flights on the KC-135A aircraft was used initially to confirm that equal numbers of pollen grains could be collected and transferred in the absence of gravity. Brassica was grown in the Plant Growth Facility flight hardware as follows. Three chambers each contained six plants that were 13 d old at launch. As these plants flowered, thin colored tape was used to indicate the date of hand pollination, resulting in silique populations aged 8-15 d postpollination at the end of the 16-d mission. The remaining three chambers contained dry seeds that germinated on orbit to produce 14-d-old plants just beginning to flower at the time of landing. Pollen produced by these plants had comparable viability (93%) with that produced in the 2-d-delayed ground control. Matched-age siliques yielded embryos of equivalent developmental stage in the spaceflight and ground control treatments. Carbohydrate and protein storage reserves in the embryos, assessed by cytochemical localization, were also comparable. In the spaceflight material, growth and development by embryos rescued from siliques 15 d after pollination lagged behind the ground controls by 12 d; however, in the subsequent generation, no differences between the two treatments were found. The results demonstrate that while no stage of reproductive development in Brassica is absolutely dependent upon gravity, lower embryo quality may result following development in microgravity.