共查询到20条相似文献,搜索用时 46 毫秒
1.
2.
Claude Szpirer Fadel Tissir Michèle Rivière Pascale Van Vooren Johanna Kela Françoise Lallemand Philippe Gabant Barbara Hoebee Karin Klinga-Levan Göran Levan Josiane Szpirer 《Mammalian genome》1999,10(1):30-34
The rat Chromosome (Chr) 2 harbors several genes controlling tumor growth or development, blood pressure, and non-insulin-dependent
diabetes mellitus. We report that the region (2q1) containing the mammary susceptibility cancer gene Mcs1 also harbors the genes encoding cyclin B1, interleukin 6 signal transducer (gp130), and proprotein convertase 1. We also
generated 13 new anonymous microsatellite markers from Chr 2-sorted DNA. These markers, as well as a microsatellite marker
in the cyclin B1 gene, were genetically mapped in combination with known markers. A cyclin B1-related gene was also cytogenetically
assigned to rat Chr 11q22-q23.
Received: 21 July 1998 / Accepted: 28 August 1998 相似文献
3.
R. S. Yeung K. H. Buetow T. Scherpbier-Heddema D. W. Bell J. R. Testa 《Mammalian genome》1996,7(6):425-428
A map of rat Chromosome (Chr) 10 was generated from 21 markers, mostly of conserved structural genes, by linkage analysis
and fluorescence in situ hybridization. The study emphasizes the proximal third of the chromosome which, until now, has been relatively devoid of markers.
Based on comparative analysis, our data suggest that genes on rat Chr 10 are conserved on mouse Chr 11, 16, 17 and human Chr
16, 5, and 17.
Received: 22 November 1995 / Accepted: 29 January 1996 相似文献
4.
P. E. Fischer N. G. Holmes H. F. Dickens R. Thomas M. M. Binns E. P. Nacheva 《Mammalian genome》1996,7(1):37-41
The abundance of CA/GT repeats in the DNA of the dog (Canis familiaris) has established the importance of polymorphic microsatellites in the development of a low density map of the canine genome.
The assignment of linkage groups of markers to chromosomes by physical mapping requires reliable cytogenetic techniques for
routine production of metaphase cells. The dog has 78 chromosomes, many of which are smaller and more contracted than those
of other mammals. Although the molecular study of inherited disease in dogs has important implications for both improved welfare
in dogs and the provision of animal models for human diseases, the small size and large number of chromosomes in the canine
genome has discouraged the inclusion of cytogenetic analysis in the planning of relevant research protocols. In this report,
Fluorescence In Situ Hybridization (FISH) techniques have been optimized for the physical mapping of probes in C. familiaris. A method to obtain a good yield of early and midmetaphases from short-term peripheral blood cultures and the optimal conditions
for hybridization and detection of probes is described. Thirteen microsatellite-containing cosmid probes from a canine genomic
library in pWE15, a highly repetitive probe (human ribosomal DNA pHr14E3), and a human X Chromosome (Chr) paint have been
mapped. Six microsatellites, two ribosomal sites, and the human paint have been assigned to specific chromosomes.
Received: 5 June 1995 / Accepted: 1 September 1995 相似文献
5.
Physical assignments of 68 porcine cosmid and lambda clones containing polymorphic microsatellites 总被引:5,自引:0,他引:5
L. J. Alexander D. L. Troyer G. A. Rohrer T. P. L. Smith L. B. Schook C. W. Beattie 《Mammalian genome》1996,7(5):368-372
Two lambda phage and 66 cosmids containing informative porcine microsatellites were assigned to 17 of 18 porcine autosomes
and the X Chromosome (Chr) by fluorescence in situ hybridization (FISH). These assignments provide additional physically anchored
markers to integrate the porcine physical and genetic maps.
Received: 2 October 1995 / Accepted: 12 December 1995 相似文献
6.
T. Eki M. Abe K. Furuya I. Ahmad N. Fujishima H. Kishida A. Shiratori T. Onozaki K. Yokoyama D. Le Paslier D. Cohen F. Hanaoka Y. Murakami 《Mammalian genome》1996,7(4):303-311
The human Chromosome (Chr) 21q22.1 region contains several genes for cytokines and neurotransmitters and the gene for superoxide
dismutase (mutant forms of which can cause familial amyotrophic lateral sclerosis). A region of approximately 5.8 Mb encompassing
D21S82 and the glycinamide ribonucleotide transformylase (GART) loci was covered by overlapping YAC clones, which were contiguously
ordered by clone walking with sequence-tagged site (STSs). A total of 76 markers, including 29 YAC end-specific STSs, were
unambiguously ordered in this 5.8-Mb region, and the average interval between markers was 76 kb. Restriction maps of the YAC
clones with rare-cutting enzymes were simultaneously prepared, and the restriction sites were aligned to obtain a consensus
restriction map of the proximal region of the 21q22.1 band. The restriction map made from 44 overlapping YACs contains 54
physically assigned STSs. By integrating the consensus map of the adjacent 1.8-Mb region, we obtained a fine physical map
spanning 6.5 Mb of human Chr 21q22.1. This map contains 24 precisely positioned end-specific STSs and 12 NotI-linking markers. More than 39 potential CpG islands were identified in this region and were found to be unevenly distributed.
This physical map and the YACs should be useful as a reference map and as a resource for further structural analysis of the
Giemsa-negative band (R-band) of Chr 21q22.1.
Received: 1 September 1995 / Accepted: 21 November 1995 相似文献
7.
Four homeobox genes that belong to the four homeobox gene clusters known in mammals have been regionally assigned to four
distinct porcine chromosomes in conserved regions between human and pig. HOXA11, HOXB6, HOXC8, and HOXD4 genes were mapped by radioactive in situ hybridization to porcine Chromosomes (Chrs) 18q21-24 (with a secondary signal in
16q14-21), 12p11-12, 5p11-12, and 15q22-23 respectively. Besides, we have also revealed the presence of a porcine homeobox
(pig Hbx24) which, although showing DNA sequence homology with a mouse gene of HOXB cluster, was located on porcine Chr 3 (3p14-13) outside the Hox clusters. To support the identity of the homeobox gene clusters
analyzed and in the light of the high sequence similarity among homeobox genes, we also localized markers known to be mapped
near each Hox cluster in human. In this way, four genes were also mapped in pig: GAPD (5q12-21), GAD1 (15q21-22), INHBA (18q24), and IGFBP3 (18q24). Mapping of HOXA11, INHBA, and IGFBP3 on pig Chr 18 constitutes the first assignments of genes on this small chromosome. These new localizations extend the information
on the conservation of four human chromosomal regions in the pig genome.
Received: 7 August 1995 / Accepted: 16 October 1995 相似文献
8.
Kira K. Lueders Rosemary W. Elliott Ingo Marenholz Dietmar Mischke Michael DuPree Dean Hamer 《Mammalian genome》1999,10(9):900-905
As a first step in determining whether there are polymorphisms in the nicotinic acetylcholine receptor (nAChR) genes that
are associated with nicotine addiction, we isolated genomic clones of the β2-nAChR genes from human and mouse BAC libraries.
Although cDNA sequences were available for the human gene, only the promoter sequence had been reported for the mouse gene.
We determined the genomic structures by sequencing 12 kb of the human gene and over 7 kb of the mouse gene. While the sizes
of exons in the mouse and human genes are the same, the introns differ in size. Both promoters have a high GC content (60–80%)
proximal to the AUG and share a neural-restrictive silencer element (NRSE), but overall sequence identity is only 72%. Using
a 6-Mb YAC contig of Chr 1, we mapped the human β2-nAChR gene, CHRNB2, to 1q21.3 with the order of markers cen, FLG, IVL,
LOR, CHRNB2, tel. The mouse gene, Acrb2, had previously been mapped to Chr 3 in a region orthologous to human Chr 1. We refined mapping of the mouse gene and other
markers on a radiation hybrid panel of Chr 3 and found the order cen, Acrb2, Lor, Iv1, Flg, tel. Our results indicate that this cluster of markers on human Chr 1 is inverted with respect to its orientation on the
chromosome compared with markers in the orthologous region of mouse Chr 3.
Received: 26 January 1999 / Accepted: 10 May 1999 相似文献
9.
We have identified four single-strand conformation variants of the bovine tumor necrosis factor alpha gene by analysis of
PCR-amplified fragments. The variants are inherited in Mendelian fashion and are informative for linkage mapping. We have
mapped the bovine gene to Chromosome (Chr) 23 in a panel of somatic cell hybrids and observed genetic linkage to the major
histocompatibility complex (BoLA) genes and microsatellite markers on bovine Chr 23 in an international bovine reference family
panel. The distribution of the alleles was determined in cattle of different breeds and of different geographical origins,
which included trypano-susceptible and trypano-tolerant cattle.
Received: 3 July 1995 / Accepted: 16 October 1995 相似文献
10.
A quantitative trait locus (QTL) for blood pressure has recently been mapped to a region of roughly 30 cM on rat Chromosome
(Chr) 2 by linkage and by the use of congenic strains. For further fine mapping of the QTL, however, closely linked chromosome
markers residing in this 30-cM region are required. In the current work, 36 new markers were generated by screening rat Chr
2-sorted DNA libraries and subsequently mapped using five F2 populations. Combining new and existing markers, the marker density for the 30-cM region approaches, on average, one marker
per 1.1 cM.
Received: 11 April 1997 / Accepted: 12 May 1997 相似文献
11.
Comparative studies of genes in the pseudoautosomal region (PAR) of human and mouse sex chromosomes have thus far been very
limited. The only comparisons that can presently be made indicate that the PARs of humans and mice are not identical in terms
of gene content. Here we describe additional comparative studies of human pseudoautosomal genes and their mouse homologs.
Using a somatic cell hybrid mapping panel, we have assigned the mouse homolog of the human pseudoautosomal interleukin 3 receptor
alpha subunit (IL3RA) gene to mouse Chromosome (Chr) 14. Attempts to clone the mouse homolog of the human pseudoautosomal
adenine nucleotide translocase-3 (ANT3) gene resulted in the isolation of the murine homologs of the human ANT1 and ANT2 genes.
The mouse Ant1 and Ant2 genes are very similar in sequence to their human homologs, and we have mapped them to mouse Chromosomes (Chrs) (8 and X
respectively) that exhibit conserved synteny with the chromosomes on which the human genes are located. In contrast, the homolog
of ANT3 appears to be either very divergent or absent from the mouse genome. Southern blot analysis of DNA from a variety
of mammalian species shows restricted conservation of human pseudoautosomal genes, a trend that also applies to the two cloned
mouse homologs of these genes and to neighboring human genes in distal Xp22.3. Our observations combined with those of other
workers lead us to propose a model for the evolution of the PAR that includes both rapid sequence evolution and the incremental
reduction in size of the region during mammalian evolution.
Received: 4 May 1995 / Accepted: 21 August 1995 相似文献
12.
Ethan A. Carver Laurie Issel-Tarver Jasper Rine Anne S. Olsen Lisa Stubbs 《Mammalian genome》1998,9(5):349-354
Olfactory receptors are G protein-coupled, seven-transmembrane-domain proteins that are responsible for binding odorants
in the nasal epithelium. They are encoded by a large gene family, members of which are organized in several clusters scattered
throughout the genomes of mammalian species. Here we describe the mapping of mouse sequences corresponding to four conserved
olfactory receptor genes, each representing separate, recently identified canine gene subfamilies. Three of the four canine
genes detected related gene clusters in regions of mouse Chromosomes (Chrs) 2, 9, and 10, near previously mapped mouse olfactory
genes, while one detected a formerly unidentified gene cluster located on mouse Chr 6. In addition, we have localized two
human gene clusters with homology to the canine gene, CfOLF4, within the established physical map of Chr 19p. Combined with
recently published studies, these data link the four conserved olfactory gene subfamilies to homologous regions of the human,
dog, and mouse genomes.
Received: 10 September 1997 / Accepted: 29 December 1997 相似文献
13.
J. Riquet D. Milan N. Woloszyn A. Schmitz F. Pitel G. Frelat J. Gellin 《Mammalian genome》1995,6(9):623-628
We have developed a simple and efficient method to construct partial libraries of swine Chromosome (Chr) 11, starting with only 300 flow-sorted copies. DNA is amplified by PARM-PCR with primer containing at the 5-end the sequence AGCU-. After amplification, digestion of PCR products with uracil DNA glycosylase generates cohesive ends corresponding to the SstI site. The amplified fragments can then be ligated in vector linearized with the SstI enzyme. Using five different primers, we PARM-PCR amplified and cloned swine Chr 11 DNA. These chromosome-specific libraries have been used to develop 14 different (TG)n microsatellites. Ten of these markers were assigned to Chr 11 by PCR analysis of a panel of Pig-Rodent somatic hybrids and by linkage analysis of the 171 individuals of the PiGMaP reference families. A complete linkage map of 147 cM of this chromosome was then realized by integrating existing markers. 相似文献
14.
Svetlana D. Pack Vladimir M. Bedanov Olga V. Sokolova Natalia S. Zhdanova Natalia M. Matveeva Oleg L. Serov 《Mammalian genome》1992,3(2):112-118
To expand the mink map, we established a new panel consisting of 23 mink-mouse clones. On the basis of statistical criteria (Wijnen et al. 1977; Burgerhout 1978), we developed a computer program for choice of clones of the panel. Assignments of the following mink genes were achieved with the use of the hybrid panel: glyoxalase (GLO), Chromosome (Chr) 1; acetyl acylase (ACY), Chr 5; creatine phosphokinase B (CKBB), Chr 10; alcohol dehydrogenase-2 (subunit B) (ADH2), Chr 8. Using a series of clones carrying rearrangements involving mink Chr 1 and 8, we assigned the gene for ME1 to the short arm of Chr 1 and that for ADH2 to Chr 8, in the region 8p12-p24. Mapping results confirm the ones we previously obtained with a mink-Chinese hamster panel. However, by means of an improved electrophoretic technique, we revised the localization of the gene for purine nucleoside phosphorylase (NP), which has been thought to be on mink Chr 2. It is reassigned to mink Chr 10. 相似文献
15.
Linkage map of seven polymorphic markers on rat Chromosome 18 总被引:8,自引:0,他引:8
Elaine F. Remmers Ellen A. Goldmuntz Hongbin Zha Leslie J. Crofford Joseph M. Cash Peter Mathern Ying Du Ronald L. Wilder 《Mammalian genome》1993,4(5):265-270
A genetic linkage map of seven polymorphic markers was created with F2 intercross progeny of F344/N and LEW/N rats and assigned to rat Chromosome (Chr) 18. Five of the markers described were defined by simple sequence length polymorphisms (SSLPs) associated with five genes: transthyretin (TTR), trypsin inhibitor-like protein (TILP), 2 adrenergic receptor (ADRB2), olfactory neuron-specific G protein (OLF), and gap junction protein (GJA1). One marker was defined by a restriction fragment length polymorphism (RFLP) detected with a probe for the human colony stimulating factor 1 receptor (CSF1R) gene. The D18N1R locus was defined by an anonymous DNA fragment amplified by the randomly amplified polymorphic DNA (RAPD) technique with a single short primer. These seven DNA loci formed a single genetic linkage group 30.4 cM in length with the following order: TTR-6.8 cM-D18N1R-9.1 cM-TILP-4.3 cM-CSF1R-0 cM-ADRB2-10.2 cM-OLF-0 cM-GJA1. The five SSLP markers were highly polymorphic. In a total of 13 inbred rat strains analyzed (F344/ N, LEW/N, LOU/MN, WBB1/N, WBB2/N, MR/N, MNR/N, ACI/N, SHR/N, WKY/N, BN/SsN, BUF/N, and LER/N), three to six alleles were detected for each marker. Remarkable linkage conservation was detected between the region of rat Chr 18 mapped and a region of mouse Chr 18. However, genes associated with these markers have been mapped to three different human chromosomes (Chrs 5, 6, and 18). The markers described here should be useful for genetic mapping studies and genetic monitoring of inbred rat strains. 相似文献
16.
Kazuhiro Yoneda Yasuo Moritomo Marika Takami Siro Hirata Yoshio Kikukawa Tetsuo Kunieda 《Mammalian genome》1999,10(6):597-600
A hereditary chondrodysplastic dwarfism caused by an autosomal recessive gene has been reported in a population of Japanese
Brown cattle. Affected calves show an insufficiency of endochondral ossification at the long bones of the limbs. In the present
study, we mapped the locus responsible for the disease (bcd) by linkage analysis, using microsatellite markers and a single paternal half-sib pedigree obtained from commercial herds.
Linkage analysis revealed a significant linkage between the bcd locus and marker loci on the distal region of bovine Chromosome (Chr) 6. The bcd locus was mapped in the interval between microsatellite markers BM9257 and BP7 or BMS511 with a recombination fraction of 0.05 and 0.06, and a lod score of 8.6 and 10.1, respectively. A comparison of genetic maps
between bovine Chr 6 and human Chr 4 or mouse Chr 5 indicates possible candidate genes including FGFR3 and BMP3 genes, which
are responsible for human chondrodysplasias and associated with bone morphogenesis, respectively.
Received: 24 November 1998 / Accepted: 2 February 1999 相似文献
17.
D. Milan N. Woloszyn M. Yerle P. Le Roy M. Bonnet J. Riquet Y. Lahbib-Mansais J. -C. Caritez A. Robic P. Sellier J. -M. Elsen J. Gellin 《Mammalian genome》1996,7(1):47-51
It has been shown that a major gene, called RN, is responsible for the RTN technological yield, a meat quality porcine trait. Experimental families informative for the segregation
of RN gene were constituted from animals belonging to the Laconie composite line. We have previously mapped the RN gene to Chromosome (Chr) 15 (Milan et al. Genet. Sel. Evol. 27, 195–199, 1995). A Chr 15 map was established with 16 markers.
The RN gene was found to be located between markers Sw120 and Sw936, at 2 cM from Sw936 (LOD = 38.1). In addition, by localizing Sw936 at 15q21–22 using DISC-PCR, we also located RN on the physical map.
Received: 25 April 1995 / Accepted: 相似文献
18.
Thirteen loci physically assigned to sheep Chromosome 2 by cell hybrid analysis and in situ hybridization 总被引:1,自引:0,他引:1
T. E. Broad P. E. Lewis D. J. Burkin A. J. Gleeson M. A. Carpenter C. Jones P. D. Pearce D. W. Maher H. A. Ansari 《Mammalian genome》1995,6(12):862-866
Sheep x hamster cell hybrids containing sheep metacentric Chromosome (Chr) 2 were produced by fusing blood leukocytes from normal sheep with hamster auxotrophic Ade F-minus mutants. Cell clones that were isocitrate dehydrogenase 1 (IDH1) positive were cytogenetically characterized, confirming that they contained sheep Chr 2. The following loci were newly assigned by Southern hybridization to sheep Chr 2: lipoprotein lipase (LPL), glycoprotein-4-beta galactosyltransferase 2 (GGTB2), neurofilament light polypeptide (68 kDa; NEFL), surfactant-associated protein 2 (SFTP2), lymphocyte-specific protein tyrosine kinase (LCK), and nebulin (NEB). These new assignments and the in situ localization of gelsolin (GSN) to sheep Chr 2pter-p24 are consistent with the predicted homology of cattle Chr 8 (U18) with sheep Chr 2p, and of cattle Chr 2 (U17) with sheep 2q. In addition, the assignment by cell hybrid analysis of loci previously mapped to Chr 2 in sheep, viz., cholinergic receptor, nicotinic, delta polypeptide (CHRND), collagen type III alpha 1 (COL3A1), fibronectin 1 (FN1), isocitrate dehydrogenase (IDH1), and villin 1 (VIL1), confirmed the localization of sheep syntenic group U11 to this chromosome. By nutritional selection and complementation of the hamster auxotrophic Ade F mutation, the multifunctional enzyme locus phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase)/IMP cyclohydrolase (inosinicase) (provisionally given the symbol PRACFT) has also been newly assigned to sheep Chr 2. This report significantly extends the number of loci physically mapped to sheep Chr 2 and confirms its close homology with cattle Chrs 2 and 8. 相似文献
19.
《Life sciences》1995,56(18):PL369-PL375
Opiate receptors are the primary targets for the drugs of abuse morphine and heroin. In this study, we completed the localization on mouse chromosomes of the genes encoding mu (Oprm) and kappa (Oprk) receptors, as well as the genes for the opioid propeptides proenkephalin (Penk) and prodynorphin (Pdyn). The genetic mapping was performed using a panel of DNA samples from an interspecific cross [C3H/HeJ-gld and (C3H/HeJ-gld x Mus spretus)Fi] that has been characterized for more than 800 markers throughout the genome. The genes are localized on mouse Chr 1 (Oprk, 10 cM from the centromere), Chr 2 (Pdyn, 75 cM from the centromere), Chr 4 (Penk, 1 cM from the centromere) and Chr 10 (Oprm, 10 cM from the centromere). Interestingly, the gene for the mu receptor is located in the same region as a Quantitative Trait Locus for high morphine consumption, thus raising the possibility of its direct role in drug abuse mechanisms. 相似文献
20.
Mass-spectrometrical analysis of proteins encoded on chromosome 21 in human fetal brain 总被引:1,自引:0,他引:1
Summary. Overexpression of chromosome 21 genes is directly or indirectly responsible for the Down syndrome phenotype. In order to analyse
chromosome 21 gene products (Chr21Ps), we extracted proteins from fetal human brain cortex and applied an ultracentrifugal
and chromatographic prefractionation principle followed by two-dimensional gel electrophoresis (2-DE) and mass-spectrometrical
analysis using high-throughput automated MALDI-TOF/TOF. Nine Chr21Ps were identified: pyridoxal kinase; superoxide dismutase
[Cu/Zn] 1; carbonyl reductase 1; ES1 protein homolog, mitochondrial [Precursor]; cystathionine-beta-synthetase; T-complex
protein 1, theta subunit; cystatin B; 6-phosphofructokinase; glycinamide ribonucleotide synthetase. Mass-spectrometric characterisation
of Chr21Ps following separation in 2-DE gels is a useful tool for the analysis of these structures in brain, independent of
antibody availability and specificity. 相似文献