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1.
Yaser Hassan Dewir Nisha Singh Shakira Shaik Ashley Nicholas 《In vitro cellular & developmental biology. Plant》2010,46(1):41-46
The present study reports a simple protocol for indirect shoot organogenesis and plant regeneration of Sutherlandia using rachis and stem segments. Different concentrations (0.0–68.08 μmol l−1) of thidiazuron (TDZ) were used for callus induction and shoot organogenesis. The highest percentage of callus formation
(97.5%) and the highest percentage of explants forming shoots (88.8%) were obtained from rachis explants cultured onto Murashige
and Skoog (MS) medium (Murashige and Skoog, Physiol. Plant. 15:473–495, 1962) supplemented with 45.41 μmol l−1 TDZ. Scanning electron microscopy demonstrated the early development of adventitious shoots derived from callus cultures.
Shoot clusters were further developed and grown in MS hormone-free medium. The presence of l-canavanine was determined by thin-layer chromatography and confirmed after column fractionation using silica gel and nuclear
magnetic resonance spectroscopy. Individual shoots were rooted on different concentrations and combinations of MS salt strength
and IBA. Half-strength MS salt medium supplemented with 24.6 μmol l−1 IBA was optimal for root induction in which 78% of shoots were rooted. The in vitro plants were successfully acclimatized in a growth chamber with a 90% survival rate. 相似文献
2.
J. Vijaya Kumar B. D. Ranjitha Kumari G. Sujatha Enrique Castaño 《Plant Cell, Tissue and Organ Culture》2008,93(1):85-96
The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower
(Carthamus
tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium
with different levels of FCF (10–50%) produced embryogenic callus. In organogenesis, 42.2% microshoots formed directly from
embryogenic callus tissues in plant regeneration medium with 40% FCF. Isolated embryogenic callus cultured on embryo induction
medium containing 40% FCF induced 50.2% somatic embryogenesis. Embryo germination percentage was decreased from 64.5 to 28
in embryo maturation medium containing 40% FCF. However, nine plantlets from organogenesis and 24 plantlets from somatic embryogenesis
were selected as FCF-tolerant. Alternaria carthami fungal spores (5 × 105 spores/ml) sprayed on the leaves of FCF-tolerant plants showed enhanced survival rate over control plants, which plants were
more susceptible to fungal attack. The number of leaf spot lesions per leaf was decreased from 3.4 to 0.9 and their lesion
length was also reduced from 2.9 to 0.7 mm in organogenic derived FCF-tolerant plants over control. In somatic embryo derived
FCF-tolerant plants, the number of lesions was decreased from 3.1 to 0.4 and the lesion size was also reduced to 2.7–0.5 mm
when compared to the control. This study also examined antioxidant enzyme activity in FCF-tolerant plants. Catalase (CAT)
activity was slightly decreased whereas peroxidase (POD) activity was increased to a maximum of 42% (0.19 μmol min−1 mg−1 protein) from organogenesis and 47% (0.23 μmol min−1 mg−1 protein) from embryogenesis in FCF-tolerant plants. Superoxide dismutase (SOD) activity was also increased to 17% (149 U
mg−1 protein) and 19.5% (145 U mg−1 protein) in FCF-tolerant plants derived from organogenesis and somatic embryogenesis when compared with control plants. 相似文献
3.
Immature inflorescences of kodo millet (Paspalum scrobiculatum L. cv. GPUK-3) were cultured on MS medium. Induction of embryogenic callus and subsequent somatic embryogenesis was possible
on both 2,4-D and Picloram alone or with kinetin from spikelets as well as rachis. Immature inflorescence cultured on medium
supplemented with lower levels of Picloram in combination with kinetin developed organogenic callus with shoot buds. Direct
somatic embryo formation on rachis was observed at higher levels of Picloram in combination with kinetin. Plant regeneration
was observed when calluses were transferred to α-napthaleneacetic acid (NAA) plus 6-benzylaminopurine (BA) supplemented MS
medium. Histological observations provided a clear evidence for both somatic embryogenesis and shoot organogenesis. Profuse
rooting was induced on phenylacetic acid (PAA) supplemented medium. Regenerated plants were successfully transferred to pots
under field conditions where most of the plants survived and set normal seeds. 相似文献
4.
R. V. Sairam C. Wilber J. Franklin B. Smith J. Bazil R. Hassel D. Whaling K. Frutiger C. A. Blakey R. Vierling S. L. Goldman 《In vitro cellular & developmental biology. Plant》2002,38(5):435-440
Summary A protocol for high-frequency callus, somatic embryogenesis, and plant regeneration for Tripsacum is described. Plants were regenerated from complete shoot meristems (3–4 mm) via organogenesis and embryogenesis. In organogenesis,
the shoot meristems were cultured directly on a high cytokinin medium comprising 5–10 mgl−1 (22.2–44.4 μM) 6-benzyladenine (BA). The number of multiple shoots varied from six to eight from each meristem. The time required for production
of plants from organogenesis was rapid (4–6 wk). In contrast, callus was induced on an auxin medium and continuously cultured
on an auxin medium for production of somatic embryos. Prolific callus with numerous somatic embryos developed within 3–4 wk
when cultured on an auxin medium containing 5 mgl−1 (22.6μM), 2,4-dichlorophenoxyacetic acid (2,4-D). The number of shoots induced varied from two to five per callus. Regardless of
the cultivars used, the frequency of callus induction and plant regeneration was between 48% and 94%. The seed germination
procedures also were modified and resulted in a maximum of 60–80% seed germination. Finally, the rate of T-DNA transfer to
complete shoot meristems of Tripsacum was high on the auxin medium and was independent of whether super-virulent strains of Agrobacterium were used or not. 相似文献
5.
High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng 总被引:4,自引:0,他引:4
W. Tang 《Plant cell reports》2000,19(7):727-732
The morphogenesis ability of light yellowish globular callus derived from cotyledons of mature zygotic embryos of Panax ginseng was investigated. The optimal media for somatic embryogenesis and shoot organogenesis were MS medium containing 0.5 mg l–1 2,4-dichlorophenoxyacetic acid, 0.1 mg l–1 6-benzyladenine (BA), and 500 mg l–1 lactoalbumin hydrolysate, and SH medium supplemented with 0.5 mg l–1 α-naphthaleneacetic acid, 0.1 mg l–1 BA, and 500 mg l–1casein hydrolysate. The influences of glucose, mannose, fructose, and sorbose in the media on somatic embryogenesis and shoot
organogenesis were revealed as differences in the numbers of somatic embryos and adventitious shoots per gram of morphogenic
callus. The best regeneration of somatic embryos was obtained on medium containing glucose, with a mean of 8.7 somatic embryos
per gram of callus. The best regeneration of shoots was observed on medium containing fructose, with an average of 12.2 adventitious
shoots per gram of callus. Of the somatic embryos 95% were converted into regenerated plantlets, and 100% of adventitious
shoots rooted to form regenerated plantlets. Regenerated plants were successfully established in soil. Flowering was observed
in 5.7% of the regenerated plants derived from shoot organogenesis and in 1.4% of the regenerated plants derived from somatic
embryogenesis.
Received: 1 December 1998 / Revision received: 13 September 1999 / Accepted: 20 September 1999 相似文献
6.
Summary Callus induction and later plant regeneration were studied in four widely grown garlic (Allium sativum L.) cultivars from Europe. Root segments from in vitro plantlets were used as starting material. In addition to cultivar effects, the effects of auxin and cytokinin levels and
the position of the segments on the root were studied. There were no statistically significant differences among cultivars
for the number of root segments that induced callus in the two series of experiments. The average induction frequency was
34.7% in the first series of experiments. Callus induction on apical root segments was significantly higher compared to callus
induction on non-apical root segments in the second series of experiments. Two months after callus induction, callus lines
were transferred to a regeneration medium consisting of Murashige and Skoog basal medium supplemented with 30gl−1 sucrose and 1 mgl−1 (4.6μM) kinetin. Calluses derived from different experiments were quite uniform with respect to their regeneration potential. Also
it was found that our regeneration system was cultivar-independent. The average shoot regeneration frequency was 17.9% in
the first series of experiments. Highly significant differences were found in the frequency of shoot regeneration among different
callus induction treatments. When the cytokinin 6-(γ,γ-dimethylallylamino)purine (0.1mgl−1∶0.5 μM) was present during callus induction, shoot regeneration ranged from 30.10 to 47.60%. Shoot regeneration from callus induced
on non-apical segments was higher, although not significant, compared to callus induction from apical root segments in the
second series of experiments. All in all, an efficient callus induction and plant regeneration system was developed from both
apical and non-apical segments taken along the entire length of the roots. This system has potential to be used for garlic
transformation. 相似文献
7.
Temperature-stress Pretreatment in Barley Anther Culture 总被引:7,自引:0,他引:7
Methods of pretreating anthers at different temperatures priorto culture have been tested, with respect to pollen-callus productionand plant regeneration, in Hordeum vulgare cv. Sabarlis. For callus production, pretreatment of excised spikes (in sealedPetri dishes) was more effective than pretreatment of excisedtillers (in water or in polythene) at both 4 and 25 °C.Pretreatment of individual anthers at these temperatures wasdeleterious. Greater callus yields resulted from pretreatmentat 4 than at 25 °C, both for spikes and tillers, 35weeks being required for maximal yields at 4 °C and 35days at 25 °C. At 4 °C, a shorter pretreatment was requiredfor spikes than for tillers. Pretreatment of spikes was alsomore effective at 4 than at 7, 14 or 20 °C. Pretreatmentof individual spikelets at 4 °C was as effective as thatof whole spikes. For plant regeneration, calluses derived from pretreatment ofspikes were more effective than those derived from pretreatmentof tillers. More plants resulted from pretreatment at 4 thanat 25 °C, both for spikes and tillers. Maximal pretreatmenttimes for plant regeneration generally exceeded those for callusproduction. Following spike pretreatment at 4 °C the maximumfor plant regeneration exceeded that for callus production byabout 2 weeks. With this optimal pretreatment approximately60 per cent of the calluses gave rise to plantlets. Among this60 per cent, for every three calluses giving albinos, two gavegreen plantlets, equivalent to five green plantlets on averagefor every 100 anthers (= two spikes) cultured. The ratio ofgreen to albino plantlets was lower for all other pretreatments. Hordeum vulgare L., barley, anther culture, pollen callus, pollen plant-production, temperature stress 相似文献
8.
Segments of young inflorescences of Triticum aestivum cv. Chinese Spring (CS), its F1 hybrids with Agropyron trachycaulum and A. scirpeum and backcross derivatives with A. yezoense, A. intermedium and A. junceum, and of a A. yezoense x T. aestivum cv. Wichita hybrid were cultured. Different parts of young spikelets of A. trachycaulum x CS F1 and A. yezoense x Wichita F1 's were also cultured. Percent callus induction was lower in wheat than in the wheat-Agropyron hybrids or backcross derivatives. Percent callus induction from different organs in both hybrids was in the descending order of whole spikelet, spikelet without glumes, rachis, and glumes. No plants could be regenerated from calli of wheat and backcross derivatives except those of CS x A. intermedium combination. Callus induction in hybrids varied from 54 to 84% and plant regeneration from 14 to 31%. The regenerants required no vernalization. Variants including one with top-dense spikes and another with elongated spikelets were recovered. Out of eight A. trachycaulm x CS hybrid regenerants, one had anthers and stigma as opposed to neutral flowers of the original hybrid. 相似文献
9.
Li Liu Xiaoli Fan Junwei Zhang Meiling Yan Manzhu Bao 《In vitro cellular & developmental biology. Plant》2009,45(6):673-680
In this study, we have demonstrated that Zoysia japonica callus induced from mature seeds can produce high frequencies of plant regeneration and somatic embryogenesis, even following
a prolonged period of subculturing. Initial callus cultures were induced from mature seeds of Japanese lawngrass (Z. japonica Steud.) incubated on a medium containing major N6 medium salts, minor Murashige and Skoog (MS) medium salts, and modified MS medium organic elements supplemented with 3 mg
L−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.01–0.02 mg L−1 6-benzyladenine. Compact callus were selected and subcultured monthly on a medium containing 2 mg L−1 2,4-D, 0.5 mg L−1 kinetin, 500 mg L−1 casein hydrolysate, 500 mg L−1 proline, and 500 mg L−1 myoinositol. Callus maintained in vitro for 18 mo could be induced to regenerate plantlets with a frequency of >90%. By contrast, 36-mo-old callus cultures failed
to produce normal shoot regeneration. However, the addition of CuSO4 to the subculture media maintained >90% regeneration frequencies in such long-term callus cultures. Histological observations
revealed that plant regeneration occurred both through somatic embryogenesis and organogenesis pathways. The ability to sustainable
regeneration in long-term callus cultures will be valuable to the program of genetic transformation and somaclonal variant
selection. 相似文献
10.
Budi Winarto Fitri Rachmawati Dewi Pramanik Jaime A. Teixeira da Silva 《Plant Cell, Tissue and Organ Culture》2011,105(3):363-374
Anthurium anther culture was successfully established using half-anthers as explants. Explants were cultured on Winarto–Teixeira basal
medium (WT-1) containing 0.01 mg/l α-naphthalene acetic acid (NAA), 0.5 mg/l thidiazuron (TDZ), and 1.0 mg/l 6-benzylaminopurine
(BAP), or on New Winarto–Teixeira basal medium (NWT-3) supplemented with 0.02 mg/l NAA, 1.5 mg/l TDZ, and 0.75 mg/l BAP for
callus initiation. Regenerated calli produced multiple shoots on WT-1, which were then rooted in NWT-3 supplemented with 1%
activated charcoal. Plantlets were acclimatized ex vitro using a mixture of burned rice husk, rice husk, and bamboo peat (1:1:1,
v/v/v) as the potting medium. There was considerable morphological and cytological diversity of regenerants derived from anther
culture, which are described in detail in this study. The callus cluster color ranged from green to light green and had a
high regeneration capacity (7.3 and 4.8 shoots/callus cluster), light reddish-yellow callus showed moderate regeneration (2.6
shoots/callus cluster), while reddish-yellow callus had the lowest regeneration capacity (1.5 shoots/callus cluster). Morphological
variations clearly observed in regenerants derived from this technique included alterations in plant size, peduncle length,
spathe position compared to leaves, the type and number of buds, spathe and spadix color, and spadix length. There were also
cytological variations in both in vitro and ex vitro regenerants of anther culture with 23–29% haploids, 5–10% aneuploids,
56–69% diploids, and 3–4% triploids. The results strengthen other studies in which the development of anther cultures, especially
via callus formation, resulted in morphological and cytological alterations. These variations have been discussed to great
length in this paper. 相似文献
11.
Karla K. Valenzuela-Sánchez Raul E. Juárez-Hernández Andrés Cruz-Hernández Víctor Olalde-Portugal María Elena Valverde Octavio Paredes-Lopez 《In vitro cellular & developmental biology. Plant》2006,42(4):336-340
Summary Indirect organogenesis was developed in Agave tequilana. Leaf segments and meristematic tissue from the central head (‘pi?a’) were evaluated as explant sources. A minimal-sized
explant with high bud-forming capacity (19.5 BFC) was obtained through a cross section of meristematic tissue from in vitro plantlets. In callus culture, the best growth response was due to naphthalene acetic-acid (NAA) presenting a contrasting
response compared to 2,4-dichlorophenoxyacetic acid (2,4-D). Regeneration from meristem segments and callus was obtained using
1.1 μM 2,4-D and 44 μM 6-benzylaminopurine (BA). The regeneration capacity of callus was maintained for 3 mo. Shoots regenerated were rooted in
a hormone-free MSI medium and acclimatized in a greenhouse with a 100% survival. 相似文献
12.
Flower buds, cotyledons and hypocotyls of Pharbitis nil were used as plant material. Flower buds (1–2 mm long) were excised from 3-week-old plants, grown in soil. Cotyledons of
7-day-old sterile seedlings were cut into 25 mm2 squares cotyledons whereas hypocotyls were cut to 1 mm long fragments. Explants were transferred into Petri dishes containing
the Murashige and Skoog medium (MS), supplemented with either BA (11 μM·L−1) alone or BA (22 μM·L−1) and NAA (0.55 μM·L−1), and different sugars: sucrose, fructose, glucose, mannose or sorbitol (autoclaved or filter-sterilized). Addition of glucose
instead of sucrose to the medium stimulated the induction of callus on flower buds and cotyledonary explants, but inhibited
its growth on fragments of hypocotyls. The medium supplemented with fructose (especially filter-sterilized) stimulated the
development of flower elements. Organogenesis of shoots and roots on explants was also observed. Flower buds and hypocotyls
were able to regenerate both organs. Addition of fructose or glucose to the medium stimulated the organogenesis of shoots,
whereas root organogenesis was inhibited on all explants used. Sorbitol strongly inhibited both induction of callus and organogenesis
on all explants used. 相似文献
13.
Ashok Kumar Sahrawat Suresh Chand 《In vitro cellular & developmental biology. Plant》2001,37(1):55-61
Summary An efficient method was established for high-frequency embryogenic callus induction and plant regeneration from 3-,4-, 5-
and 7-d-old coleoptile segments of Indica rice (Oryza sativa L. cv. Kasturi), Compact and friable callus developed from the cut ends and also on the entire length of the coleoptile segments
cultured on Murashige and Skoog (MS) basal medium (1962) supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 4.50–18.0
μM), kinetin (2.32 μM) and sucrose (3%, w/v). High frequency embryogenic callus induction and somatic embryo development was achieved when embryogenic
calluses were transferred to MS medium supplemented with 2.25 μM 2,4-D, 2.32 μM kinetin, 490 μM
L-tryptophan and 3% (w/v) sucrose. Plant regeneration was achieved by transferring clumps of embryogenic callus onto MS medium
containing 2.85 μM indole-3-acetic acid (IAA), 17.77 μM 6-benzylaminopurine (BA) and 3% (w/v) sucrose. Histological observations of embryogenic calluses revealed the presence of
somatic embryos and also plant regeneration via multiple shoot bud formation. Three, 4- and 5-d-old coleoptile segments showed
a significantly (P<0.05) higher frequency of plant regeneration and mean number of plantlets per explant in comparison to 7-d-old coleoptile
segments. The highest frequency (73.5%) of plant regeneration and mean number of plantlets (11.9±1.0) was obtained from 4-d-old
coleoptile segments. Regenerated shoots were rooted on MS basal medium containing 4.92 μM indole-3-butyric acid (IBA) and plants were successfully transferred to soil and grown to maturity. 相似文献
14.
Morphogenesis and regeneration from stomatal guard cell complexes of cotton (Gossypium hirsutum L.) 总被引:1,自引:0,他引:1
The development of a regeneration system from cotton stomatal guard cells directly on epidermal strips is described. The
most important factors affecting embryogenic callus initiation in both of the varieties tested (Coker 312 and 315) were the
source of the epidermal tissue, including plant age (4–5 months old), the developmental stage of the flower (opening flower
stage) from which bracts were obtained, the composition of the culture medium and light irradiance. The flower developmental
stage was critical for callus formation, which was observed only from bracts obtained from opening flowers. In addition, epidermal
strips excised from the bract basal region were more responsive in culture than those obtained from the top region. Improved
callus initiation was obtained on epidermal strips which had their cuticle in contact with the culture medium. Light irradiance
was a limiting factor for embryogenic callus formation, which was observed only in calluses cultured under the lower light
irradiance (15.8 μmol m–2 s–1). Somatic embryogenesis was observed on callus cultures subcultured consecutively to a culture medium containing naphthalene
acetic acid (10.7 μM) and isopentenyladenine (4.9 μM). Histodifferentiation of somatic embryos was improved on a medium containing naphthaleneacetic acid (8.1 μM)+isopentenyladenine (2.5 μM) and abscisic acid (0.19–0.38 μM). Somatic embryo germination and plantlet development were obtained using established protocols with few modifications. On
average, one fully developed plant was obtained from the culture of circa 100 epidermal strips in both cultivars.
Received: 19 May 2000 / Revision received: 25 August 2000 / Accepted: 29 August 2000 相似文献
15.
S. C. Debnath 《In vitro cellular & developmental biology. Plant》2009,45(2):122-128
An efficient system to regenerate shoots on excised leaves of greenhouse-grown wild lowbush blueberry (Vaccinium angustifolium Ait.) was developed in vitro. The effect of thidiazuron (TDZ) on adventitious bud and shoot formation from apical, medial, and basal segments of the leaves
was tested. Leaf cultures produced multiple buds and shoots with or without an intermediary callus phase on 2.3–4.5 μM TDZ
within 6 wk of culture initiation. The greatest shoot regeneration came from young expanding basal leaf segments positioned
with the adaxial side touching the culture medium and maintained for 2 wk in darkness. Callus development and shoot regeneration
depended not only on the polarity of the explants but also on the genotype of the clone that supplied the explant material.
TDZ-initiated cultures were transferred to medium containing 2.3–4.6 μM zeatin and produced usable shoots after one additional
subculture. Elongated shoots were dipped in 39.4 mM indole-3-butyric acid powder and planted on a peat:perlite soilless medium
at a ratio of 3:2 (v/v), which yielded an 80–90% rooting efficiency. The plantlets were acclimatized and eventually established in the greenhouse
with 75–85% survival. 相似文献
16.
An efficient in vitro regeneration protocol was developed for medicinally important aromatic plant Anethum graveolens. Nodal segments were cultured onto Murashige and Skoog (MS) basal medium supplemented with different auxins and cytokinins
singly as well as in combinations. The optimum callus induction (93.33 %) was obtained on medium fortified with 2.2 μM N6-benzyladenine (BA) and 0.21 μM α-naphthaleneacetic acid. The best shoot regeneration (85.7 %) with 12.86 shoots per explant
was achieved in two weeks when callus was subcultured on MS medium amended with 2.2 μM BA and 1.85 μM kinetin. The average
length of regenerated shoots varied from 3.15 to 4.8 cm. The rooting of regenerated shoots was nearly 100 % on ? MS augmented
with 4.9 μM indolebutyric acid with a maximum root length of 5.1 cm. Plantlets were successfully acclimatized with 60 % survival
rate. During organogenesis, catalase and ascorbate peroxidase activity increased while superoxid dismutase activity decreased.
Clonal fidelity of in vitro raised plants has been checked by random amplified polymorphic DNA using 10 selected decamer primers. It has been found that
regenerated plants are true to type plants. 相似文献
17.
Crown and leaf slices of in vitro plantlets of a non-flowering Vetiveria zizanioides from Java were used to induce compact calli and to regenerate plantlets. The influence of different growth regulators (2,4-dichlorophenoxy
acetic acid, 6-benzylaminopurine), sucrose concentrations (10–100 g l−1), cultivation in light or dark, and cultivation time on callus induction medium (6 or 12 weeks), on the induction of compact
callus and the subsequent regeneration of plantlets was studied. Up to 75% of crown slices cultured on modified Murashige
and Skoog medium supplemented with 2.26 μM 2,4-dichlorophenoxy acetic acid, 2.22 μM 6-benzylaminopurine and 75 g l−1 sucrose developed compact callus. For subsequent regeneration of plantlets, callus induction in the light for 6 weeks on
the callus induction medium containing 10 g l−1sucrose, and subsequent transfer to the regeneration medium, was the best procedure, regenerating plantlets on around 60%
of the crown or leaf slices, with up to 100 plantlets per slice. We have compared the efficiency of the above mentioned procedure
with several other methods to regenerate plantlets. Our findings indicate that the procedure developed in this study was best
in regenerating plantlets for the used vetiver variant.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
18.
19.
Efficient plant regeneration was achieved from callus derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.). Calli were obtained on MS media containing 3% sucrose and different concentrations of TDZ. The highest rate of green,
compact and nodular callus was formed on MS medium supplemented with 1 mg/l of TDZ. Shoot organogenesis was achieved when
the callus was transferred onto MS media containing 3% sucrose and BA alone (05–4 mg/l) or BA (0.5 and 1 mg/l) combined with
NAA or IAA (0.5 and 1 mg/l). Maximum organogenesis was obtained with 1 mg/l BA in combination with 0.5 mg/l NAA. Rooting of
the shoots was achieved on MS medium supplemented with 0.2 mg/l IBA. Regenerated plantlets were acclimatized and successfully
transplanted to soil. 相似文献
20.
Several grass species of the genus Miscanthus are considered to be outstanding candidates for a sustainable production of biomass to generate renewable energy. The purpose
of this study was to investigate the effects of genotype, the developmental stage of the explant donor inflorescence and the
induction medium on the success rate of micropropagation. The experiments were conducted on three genotypes of M. sinensis and one of M. x giganteus. Explants from the youngest inflorescences (0.1–2.5 cm in length) showed a significantly higher callus induction rate than
those from more developed inflorescences (2.6–5 cm in length). In addition, cultures initiated from explants from the youngest
inflorescences showed significantly the highest rates of callus regeneration and the highest shoot regeneration rate. Three
out of the four genotypes tested showed the best shoot regeneration from calli initiated from the youngest inflorescences
when cultured on the Murashige and Skoog basal medium (MS) with 5 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l−1 6-benzyladenine (BA). The percentages of calli from those genotypes showing regeneration ranged from 45 to 76.7%, and the
corresponding shoot regeneration rates ranged from 1.85 to 6.33 shoots/callus. This demonstrates that, with some adjustments,
efficient micropropagation of Miscanthus sp. is feasible. 相似文献