Diet of stoats at Okarito Kiwi Sanctuary, South Westland, New Zealand

The diet of 871 stoats (Mustela erminea) caught within the Okarito Kiwi Sanctuary, South Westland, New Zealand, between 2001 and 2004 was studied by assessment of gut contents. Stoat and ship rat (Rattus rattus) captures were used as a measure of relative abundance over time, and rat and mouse (Mus musculus) abundance was indexed using tracking tunnels between spring 2002 and winter 2004. There were major increases in rat captures in spring of 2002 and again in spring of 2003. Stoat captures peaked in the following summers, as rat captures declined. Rats and invertebrates were major components of stoat diet, occurring in 41% and 52% of guts respectively; birds were found in 19%. Changes in these percentages with time indicated that stoats shifted their diet from rats to birds and invertebrates as rat abundance (as indexed by tracking tunnels) decreased. The greatest impact on native species is therefore likely to be when rat abundance is declining after major irruptions, as the increased consumption of birds and invertebrates overlaps with the periods of highest stoat abundance.     

Trappability and densities of stoats (

Stoat (Mustela erminea) density was estimated by live-trapping in a South Island Nothofagus forest, New Zealand, at 8-9 (Jan/Feb 1996) and 15-16 (Aug/Sep 1996) month intervals after significant beech seedfall in autumn 1995. Absolute densities were 4.2 stoats per km² (2.9-7.7 stoats per km², 95% confidence intervals) in Jan;Feb 1996 and 2.5 stoats per km² (2.1-3.5 stoats per km²) in Aug/Sep 1996. Trappability of stoats increased in the latter sampling period, probably because mice (Mus musculus) had become extremely scarce. accordingly, trapping rates of stoats may vary temporally and spatially with food supply rather than only with absolute abundance. Ship rats (Rattus rattus) capture rates doubled between Jan/Feb 1996 and Aug/Sep 1996, but rapidly declined shortly afterwards. Trappability of ship rats also increased in the latter sampling period. These factors must be considered when planning methods of indexing relative densities of stoats and rats.     

Secondary poisoning of stoats after an aerial 1080 poison operation in Pureora Forest, New Zealand

Stoats were monitored by three methods through an aerial 1080 poisoning operation at Waimanoa, Pureora Forest in August 1997. Tracking rates and number of live captures were used as indices of abundance, and radio-transmitters were used to follow individual animals. All 13 stoats with radio-transmitters within the poisoned area died between 2-18 days after the operation. No mustelids were tracked or live-trapped after the operation for three months. Of the radio-tracked stoats that died, rat remains occurred in 67%, passerine birds in 17%, cave weta in 17% and possum in 8%. Residues of 1080 were found in 12 of the 13 dead stoats. Our findings have important implications for the management of threatened species. Stoats are known to be a major factor in the continuing decline of some native birds. Previously, the potential of secondary poisoning to control stoats land other predators) in New Zealand had focused on the use of anticoagulants, as these compounds persist and can accumulate in predators over a longer period. However, our results suggest that secondary poisoning with an acute toxin can also be highly efficient. This may also have greater public acceptability.     

Pathogen's level and parasitism rate in Ips typographus at high population densities: importance of time

We compared the levels of pathogen infection in parental beetles, parasitism of the offspring, abundance of predators and breeding performance success of univoltine populations of Ips typographus in plots characterized by short‐term (2–3 years) outbreaks vs. those with long‐term (>10 years) outbreaks on two localities at ca. 1100 m altitude in the ?umava Mts. The numbers of I. typographus were high in all plots, whether the plots were characterized by long‐term outbreaks or short‐term outbreaks. The numbers of maternal galleries in the sample areas ranged from 300 to 400 per m². The density of parental beetle galleries, abundance of surviving specimens of I. typographus, and length of maternal galleries did not differ between plots. The short‐term outbreaks had only fewer ectoparasitoids of I. typographus and a lower percentage of parasitism and infection level of Mattesia schwenkei than the long‐term outbreaks even though the maternal gallery densities and beetle production were the same. The most mortality appeared to be caused by intraspecific larval competition, and the identical reproductive success in plots with short‐term and long‐term outbreaks indicates that the negative feedback resulting from parasitoids and entomopathogens does not substantially reduce beetle numbers. Although entomopathogenic fungi as Beauveria bassiana occur naturally in the galleries of spruce bark beetles, there was no evidence of its presence in the studied population. The low levels of predation and/or parasitism in both kinds of plots indicate that natural enemies did not play a significant role in reducing outbreak numbers of I. typographus.     

Metabolic shift analysis at high cell densities

Abstract: In high cell density cultures it is virtually inevitable that the environment to which the cells are exposed is heterogeneous. Thus, with suspended cultures, individual cells are subject to temporal changes in their environment whereas with aggregated or immobilized cells, the culture can be considered as being formed by a number of subpopulations, each with its own environmental characteristics. In addition, in a high cell density environment, high concentrations of end products may negatively influence the growth rate. This may result in the selection of organisms with an altered metabolic behaviour or with a decreased sensitivity to the adverse effects of the product. We discuss the consequences of this heterogeneity with regard to carbon source metabolism in view of the ability of many bacterial species to adapt to environmental conditions. Selection of variant organisms was found to occur with Clostridium butyricum when grown for a prolonged time in a medium containing approx. I-50 mM glucose. In contrast to the original strain, these variants could sustain a high maximal growth rate in the presence of butyric acid. In addition, they had acquired the capacity to spontaneously form aggregates and were able to carry out a completely solventogenic fermentation. Heterogeneous metabolic activity in aggregated cells is demonstrated with cultures of Lactobacillus laevolacticus , an aggregateforming lactic acid bacterium that converts glucose completely to o-lactate. By using microelectrodes, we show that the fraction of metabolically active cells decreases with increasing aggregate size: in larger aggregates steep pH gradients occur with the effect that only the outer layer of the aggregate is metabolically active, i.e. contributes to lactic acid formation, whereas with smaller aggregates all cells remain active. As a result, the net specific lactic acid production rate of the population as a whole is not invariably increased with increased aggregate size.     

Spring home range, spatial organisation and activity of stoats Mustela erminea in a South Island Nothofagus forest, New Zealand

This radio-tracking study reports the spring home range, spatial organisation and activity of 11 stoats Mustela erminea in a New Zealand Nothofagus forest, 1,5 yr after significant seedfall when rodent density was low, but stoat density was high. The average home range of 4 male stoats was 223 (SE = 45) ha, significantly larger than the average range area of 94 (SE = 13) ha recorded for 7 female stoats. Stoats were generally tolerant of sharing space and did not maintain intra- or intersexual territorial spacing systems. There was no evidence of temporal avoidance with several stoats of the same sex showing slight attraction to one another. However, stoats still may avoid one another when in close proximity. Long-term radio-tracking studies are required to determine the general patterns of spacing behaviour in stoats, Male stoats showed higher levels of activity during daylight than females.     

Human respiration at rest in rapid compression and at high pressures and gas densities

Ventilation (V), end-tidal PCO2 (PACO2), and CO2 elimination rate were measured in men at rest breathing CO2-free gas over the pressure range 1-50 ATA and the gas density range 0.4-25 g/l, during slow and rapid compressions, at stable elevated ambient pressures and during slow decompressions in several phases of Predictive Studies III-1971 and Predictive Studies IV-1975. Inspired O2 was at or near natural O2 levels during compressions and at stable high pressures; it was 0.5 ATA during decompressions. Rapid compressions to high pressures did not impair respiratory homeostasis. Progressive increase in pulmonary gas flow resistance due to elevation of ambient pressure and inspired gas density to the He-O2 equivalent of 5,000 feet of seawater was not observed to progressively decrease resting V, or to progressively increase resting PACO2. Rather, a complex pattern of change in PACO2 was seen. As both ambient pressure and pulmonary gas flow resistance were progressively raised, PACO2 at first increased, went through a maximum, and then declined towards values near the 1 ATA level. It is suggested that this pattern of PACO2 change results from interaction on ventilation of 1) increase in pulmonary resistance due to elevation of gas density with 2) increase in respiratory drive postulated as due to generalized CNS excitation associated with exposure to high hydrostatic pressure. There may be a similar interaction between increased gas flow resistance and increase in respiratory drive related to nitrogen partial pressure and the narcosis resulting therefrom.     

Thermoregulatory adjustments in squirrel monkeys exposed to microwaves at high power densities

The present study was undertaken to investigate the thermal adjustments of squirrel monkeys exposed in a cold environment to relatively high energy levels of microwave fields. The animals (Saimiri sciureus) were equilibrated for 90 min to a cool environment (Ta = 20 degrees C) to elevate metabolic heat production (M). They were then exposed for brief (10-min) or long (30-min) periods to 2,450-MHz continuous-wave microwaves. Power densities (MPD) were 10, 14, 19, and 25 mW/cm2 during brief exposures and 30, 35, 40, and 45 mW/cm2 during long exposures (rate of energy absorption: SAR = 0.15 [W/kg]/[mW/cm2]). Individual exposures were separated by enough time to allow physiological variables to return to baseline levels. The results confirm that each microwave exposure induced a rapid decrease in M. In a 20 degree C environment, the power density of a 10-min exposure required to lower M to approximate the resting level was 35 mW/cm2 (SAR = 5.3 W/kg). During the long exposures, 20 min was needed to decrease M to its lowest level. Cessation of irradiation was associated with persistence of low levels of M for periods that depended on the power density of the preceding microwave exposure. Vasodilation, as indexed by changes in local skin temperature, occurred at a high rate of energy absorption (SAR = 4.5 W/kg) and was sufficient to prevent a dramatic increase in storage of thermal energy by the body; vasoconstriction was reinstated after termination of irradiation. Patterns of thermophysiological responses confirm the influence both of peripheral and of internal inputs to thermoregulation in squirrel monkeys exposed to microwaves in a cool environment.     

Geological subsidence, river capture, and cladogenesis of galaxiid fish lineages in central New Zealand

We used mtDNA and isozyme analysis of a freshwater fish, Galaxias divergens (Osmeriformes: Galaxiidae), to test a hypothesis of drainage evolution in South Island, New Zealand. Geological evidence indicates that the presently north-flowing Kaituna River branch of the Pelorus River system once flowed south into the Wairau River system. The subsequent flow-reversal is thought to have resulted from Pleistocene subsidence in central New Zealand. mtDNA sequence data corroborated this geological hypothesis: rivers draining into Pelorus Sound were found to retain a genetic lineage of G. divergens that is otherwise restricted to the Wairau River system and adjacent coastal drainages (based on current sampling). Other sampled drainages in northern South Island and southern North Island were found to house lineages that were highly divergent from the Wairau–Pelorus clade. Isozyme data yielded groupings based on fixed differences that were largely congruent with mtDNA clades. Standard molecular calibrations suggest that vicariant isolation of Pelorus and Wairau systems (drainage reversal) occurred in the mid-Pleistocene rather than the late Pleistocene as suggested by geology. Future multidisciplinary analyses will aim to improve our understanding of geological and molecular evolutionary rates.  © 2006 The Linnean Society of London, Biological Journal of the Linnean Society, 2006, 88 , 367–376.     

Scats and den contents as indicators of the diet of stoats (Mustela erminea) in the Tasman Valley,South Canterbury,New Zealand

Stoats are significant predators of native fauna in New Zealand. They occur in many habitat types and consume a wide range of prey. The diet of stoats in the Tasman River, South Canterbury, was studied by analysis of scats and den contents. Analysis of 206 scats showed that stoats ate mainly lagomorphs, birds and invertebrates. Minor components included mice, lizards, fish and hedgehogs. Stoats ate more birds in spring than in autumn, and female stoats ate more invertebrates than did males. The contents of 219 dens collected in the same area at the same time provided further information. Birds and lagomorphs occurred at high frequency in dens, and other components were minor. Remains in dens were larger than in scats and allowed identification of many more prey items to species level. Den contents revealed a potentially substantial impact of stoats on threatened shorebirds locally; this impact was not detected by analysis of scats.     

Retinoblastoma mutation rate in New Zealand and support for the two-hit model

Summary Age-specific incidence rates for 96 New Zealand patients with sporadic retinoblastoma peaked earlier for bilateral patients than for unilateral patients. The cumulative log survival until diagnosis for bilateral and unilateral patients followed linear and quadratic curves respectively, and supported the two-hit hypothesis for retinoblastoma. The germ cell mutation rate for retinoblastoma, assuming a single major gene, was calculated to be in the order of 9.3×10^-6 to 10.9×10^-6 for the New Zealand population.     

Decline in male mouse pheromone with age

An age-related decline in urinary-borne pheromone was found in male C57BL/6J mice aged from 2 to 30 months. Pheromone activity, estimated by bioassay, declined sharply after about 10 months of age. Two other strains of mice tested (DBA/2J and CBA/HT6J) also appeared to show an age-related decline in pheromone activity. Within each strain, however, pheromone activity was consistently similar to or higher than that of the C57BL/6J male mice. The DBA/2J and BALB/cWt strains appeared to be high pheromone producers, and the C57BL/6J and CBA/HT6J strains, low producers. This report is the first demonstration of a decline with age in male mouse pheromone activity. This decline appears to be synchronized with the well-defined loss of reproductive function in female mice.     

Decline in symbiont densities of tropical and subtropical scleractinian corals under ocean acidification

Coral Reefs - Ocean acidification changes the carbonate chemistry of seawater in a manner that reduces the biomineralisation rate of reef-building corals. Other effects of acidification on coral...