Bird Abundance in Different-Aged Stands of Rimu (

The abundance of birds in three different-aged stands (young, mature, and old) was examined at North Okarito, a lowland rimu (Dacrydium cupressinum) forest in Westland, using 5-minute counts, transect counts, and mist-netting. Most of New Zealand's common forest bird species were present in the study area, with relatively high numbers of brown creeper (Mohoua novaeseelandiae) and New Zealand robin (Petroica australis), and low numbers of kaka (Nestor meridionalis) and yellow- crowned parakeet (Cyanoramphus auriceps). Most insectivorous species were more abundant than expected (from sampling effort) in young and mature stands, the frugivorous New Zealand pigeon (Hemiphaga novaeseelandiae) was more abundant than expected in mature and old stands, and most omnivorous species, viz., bellbird (Anthornis melanura), silvereye (Zosterops lateralis), and tui (Prosthemadera novaeseelandiae), were more abundant than expected in young and old stands. North Okarito Forest provided an important source of seasonal foods (nectar, fruit, and seeds) for frugivorous, omnivorous, and introduced granivorous species, which tended to have greater changes in their seasonal abundance than did insectivorous species. Coupe- logging of old stands will affect all bird species because it will reduce the overall area of standing forest, but it will have a greater impact on the pigeon, bellbird, silvereye, robin, and tui because of their preference for old stands.     

Modelling the recovery of resident shorebirds following a fox eradication program using citizen science data

Historically, the land-based threat to shorebird colonies on Phillip Island, Victoria, Australia, was fox predation. As a result, a fox eradication programme consisting of three phases: knock-down (i.e., 2006), clean-up (i.e., 2011), and post-eradication. In 2011 an effective knock-down was declared, signalling the beginning of the clean-up phase. The purpose of this research is to assess the recovery of six resident shorebird species on Phillip Island following fox removal. The statistical methodologies used are novel for assessing bird species population recovery following a successful predator eradication program. We used citizen science data from 2003 to 2017, extracted from the Atlas of Living Australia. The first analysis method used INLA modelling, which relied on a Negative Binomial distribution for bird counts to look for upward trends in shorebird populations during the fox eradication operation. The second method use changepoint analysis techniques to see whether successive phases of the eradication process were associated with changes in bird population numbers. Four of the six shorebird species investigated responded positively to reduced fox populations over the 15-year study, and all changepoint approaches consistently recognised the start of the clean-up phase, with less consistency identifying the start of the knock-down phase. Since 2006, the INLA models indicate a significant increase in the upward trend of shorebird populations for three of the six shorebird species investigated. Agreement across the four changepoint techniques indicates that changes in bird numbers were associated with the date of the eradication program's clean-up phase for all of these shorebird species. These results demonstrate some promise for these methods to monitor native species recovery during eradication programs.     

Diet and impacts of brushtail possum populations across an invasion front in South Westland, New Zealand

Impact of the irruptive fluctuation in abundance of brushtail possum populations since their initial colonisation was investigated in the forests of South Westland, New Zealand. Possum abundance, fecundity, and diet, the condition of common possum-palatable tree species, and the abundance of common forest birds were measured at three sites occupied by possums for c. 10, 20, and 30 years. Possum densities were highest at the site where possums had been present for c. 20 years. Possum fecundity was higher at the site they had recently colonised than at the two sites where they had been present for c. 20–30 years. Diet of possums where they had recently colonised was dominated by highly preferred foods (72% of total diet), whereas these same foods contributed just 36% of total diet at the site where possums had been present for more than two decades. Canopy condition of common possum-preferred trees was scored progressively lower in areas with increasing length of possum occupation, especially at the site where the possum population had apparently declined from its maximum density. Native forest bird abundance also declined with increasing length of possum occupation. The implications of these results for management are discussed.     

Plant-Species Preferences of Birds in Lowland Rimu (

Plant species preferences of birds were determined by comparing the proportional bird use of plant species during direct observations with the proportions of plant species present on point-height intercepts in lowland rimu (Dacrydium cupressinum) forest in North Okarito, Westland. Plant species and bird use of plant species were divided into 5 m height classes, and rimu trees were divided into four age classes (sapling, pole, mature, and old). The frugivorous New Zealand pigeon (Hemiphaga novaeseelandiae) used mature and old rimu more than expected from the proportion of these age classes present, and it preferred the upper tiers of the forest. The omnivorous tui (Prosthemadera novaeseelandiae) had similar preferences to the pigeon. Other omnivorous species, viz., the bellbird (Anthornis melanura) and silvereye (Zosterops lateralis), shared preferences with both the pigeon and insectivorous species. Most insectivorous species, viz., the brown creeper (Mohoua novaeseelandiae), grey warbler (Gerygone igata), New Zealand fantail (Rhipidura fuliginosa), New Zealand tomtit (Petroica macrocephala), and rifleman (Acanthisitta chloris), used pole rimu more than expected and preferred the middle and lowest tiers. The insectivorous New Zealand robin (Petroica australis) had no preference for any plant species but had a strong preference for deadwood and the lowest tier of the forest. Of the 10 species sufficiently abundant to be monitored, the pigeon, bellbird, and tui are most likely to be detrimentally affected by selective-logging of mature and old rimu.     

Whey proteins of the common brushtail possum (Trichosurus vulpecula): isolation, characterization and changes in concentration in milk during lactation of transferrin, alpha-lactalbumin and serum albumin

1. Transferrin and serum albumin were purified from both whey and serum and alpha-lactalbumin was purified from whey from the common brushtail possum (Trichosurus vulpecula). 2. The N-terminal amino acid sequences for transferrin and serum albumin were identical for the proteins from both whey and serum and showed homologies with transferrin or serum albumin from other species. 3. N- and C-terminal regions of possum alpha-lactalbumin were also sequenced and have been compared with wallaby alpha-lactalbumin and several eutherian alpha-lactalbumins. 4. Antisera raised to each of the three proteins were species specific and Western blots further confirmed the identity of the serum and whey transferrins and serum and whey serum albumins. 5. The concentration of transferrin increased ten-fold between days 110 and 130 of lactation, whereas no significant changes in the concentration of alpha-lactalbumin could be detected after day 60.     

Impacts of Aerial 1080 Poisoning on the Birds of Rangitoto Island, Hauraki Gulf, New Zealand

Bird populations were monitored for one year (October 1990- October 1991) to determine whether the 1080 poison used to eradicate possums and wallabies on Rangitoto Island had had any detrimental effects on them. There was no significant decline in bird numbers recorded immediately after poisoning, with four species increasing in abundance (P <0.001). Twelve months after the operation the abundance of four species had increased significantly (P <0.001). The poisoning does not appear to have had any negative effect on the bird populations of Rangitoto, while the removal of browsing mammals may in future prove to have beneficial effects.     

Ship rat demography and diet following possum control in a mixed podocarp–hardwood forest

Control of one pest species may permit increases in abundance of other pests, thereby reducing the overall net benefit from pest control. We provide evidence that control of introduced possums (Trichosurus vulpecula) may increase ship rat (Rattus rattus) abundance in some New Zealand native forests. Ship rat abundance in a podocarp–hardwood forest was assessed using simple interference indices over 14 years (1990–2004) that included two aerial possum-poisoning operations (1994, 2000). Ship rat demography and rat and possum diet were measured from June 2001 to June 2003 when the rat population was increasing after the 2000 poisoning. Mean ship rat abundance indices increased nearly fivefold after possum control and remained high for up to 6 years after the 1994 poisoning. Rat fecundity was high (50–100% of adult females breeding), even during winter, and young animals dominated the population (73% in age classes 1–3) in 2001–2002 when rat numbers were increasing. During 2002–2003, rat abundance stabilised, without marked winter or spring reductions, the population aged significantly (only 32% in age classes 1–3), and fecundity declined to low levels (4–27% of adult females breeding). Although seeds and fruit dominated the diet of rats driving population recovery after control (74.0% of total diet by dry weight), rat fecundity was instead closely correlated with the proportional consumption of invertebrates (r = 0.91). Juvenile survival was correlated with proportional seed consumption (r = 0.75), while adult survival was correlated with combined seed and invertebrate consumption (r = 0.83). Adult rats ate more seeds and fewer invertebrates than juvenile rats. Seeds and fruit also dominated possum diet (52.2% of total diet). These results are consistent with the hypothesis that increased rat abundance following possum control is a consequence of greater availability of, or reduced competition for, seeds and fruit.     

Using soundscape recordings to estimate bird species abundance, richness, and composition

ABSTRACT Point counts are the most frequently used technique for sampling bird populations and communities, but have well‐known limitations such as inter‐ and intraobserver errors and limited availability of expert field observers. The use of acoustic recordings to survey birds offers solutions to these limitations. We designed a Soundscape Recording System (SRS) that combines a four‐channel, discrete microphone system with a quadraphonic playback system for surveying bird communities. We compared the effectiveness of SRS and point counts for estimating species abundance, richness, and composition of riparian breeding birds in California by comparing data collected simultaneously using both methods. We used the temporal‐removal method to estimate individual bird detection probabilities and species abundances using the program MARK. Akaike's Information Criterion provided strong evidence that detection probabilities differed between the two survey methods and among the 10 most common species. The probability of detecting birds was higher when listening to SRS recordings in the laboratory than during the field survey. Additionally, SRS data demonstrated a better fit to the temporal‐removal model assumptions and yielded more reliable estimates of detection probability and abundance than point‐count data. Our results demonstrate how the perceptual constraints of observers can affect temporal detection patterns during point counts and thus influence abundance estimates derived from time‐of‐detection approaches. We used a closed‐population capture–recapture approach to calculate jackknife estimates of species richness and average species detection probabilities for SRS and point counts using the program CAPTURE. SRS and point counts had similar species richness and detection probabilities. However, the methods differed in the composition of species detected based on Jaccard's similarity index. Most individuals (83%) detected during point counts vocalized at least once during the survey period and were available for detection using a purely acoustic technique, such as SRS. SRS provides an effective method for surveying bird communities, particularly when most species are detected by sound. SRS can eliminate or minimize observer biases, produce permanent records of surveys, and resolve problems associated with the limited availability of expert field observers.     

Thirty years of vegetation dynamics in response to a fluctuating rabbit population on sub‐Antarctic Macquarie Island

Australia's sub‐Antarctic Macquarie Island is presently undergoing one of the most ambitious vertebrate pest eradication programmes ever undertaken. The anticipated success of this programme will release the island's tundra‐like vegetation from well over a century of grazing and disturbance from House Mouse (Mus musculus), Ship Rat (Rattus rattus) and most significantly European Rabbit (Oryctolagus cuniculus). This study describes results from 30 years of vegetation quadrat monitoring (prior to the most recent and comprehensive pest eradication programme) when lower level pest animal control programmes were underway. Plant species were assigned to one of five distinct functional plant groups: Indigenous short‐lived perennials, Introduced short‐lived perennials, Indigenous perennials rarely grazed by rabbits, Indigenous perennials occasionally grazed by rabbits and Indigenous long‐lived perennials heavily grazed by rabbits, with one species, Agrostis magellanica, analysed as a sixth monospecific group. Results from monthly rabbit counts were used to compare changes in abundance of these six groups under different rabbit populations. It was found that there were three distinct phases of rabbit activity during the study period, indicated by (i) an initial very high count year in 1980–1981, followed by (ii) 20 years of low counts ending in 2001–2002 after which (iii) counts rose to medium/high until the commencement of the eradication programme in 2010–2011. Vegetation composition and progression were distinct for these three rabbit count phases. The first four of the plant functional groups decreased under lower count periods and increased in cover under higher rabbit count periods. Agrostis magellanica appears to respond primarily to interspecies competition and is disadvantaged under extended periods of low rabbit numbers. Indigenous long‐lived perennials heavily grazed by rabbits, which includes the large tussocks and megaherbs, is inversely related to rabbit numbers. During the study period, there has also been an overall decline in plant species richness with average species count per quadrat falling by between 0.6 and 2.7 taxa. This study attempts to address the observed vegetation change from this long‐term monitoring, to discuss other potential contributing factors and to use the results to predict likely future vegetation changes after eradication of vertebrate pests.     

The distribution and abundance of aerial seabirds in relation to Antarctic krill in the Prydz Bay region,Antarctica, during late summer

Summary The distribution patterns of aerial seabirds are analysed from counts made in the Prydz Bay region, Antarctica, during the African legs of SIBEX I and II in late summer (end of February to April), and compared with those made farther west at the same time of year during FIBEX. Species composition and abundances were similar in all three surveys, with sooty shearwaters Puffinus griseus contributing approximately half of the total aerial bird energy demand. Differences between surveys are explained in terms of longitudinal or seasonal differences in sampling areas and periods. Correlations between bird distribution patterns and environmental parameters are used to infer the scale-dependent factors affecting bird dispersion at sea. Two macro-scale bird assemblages, identified by physical parameters, were separated along latitudinal gradients (temperature and salinity) associated with the Antarctic Divergence. These assemblages are consistent with the Intermediate and Southern High Latitude Groups identified during FIBEX. At smaller spatial scales, almost all species were correlated with the abundance of Antarctic krill Euphausia superba, both across the entire SIBEX I grid, and within the areas north and south of the Antarctic Divergence. Similarly, during SIBEX II, seabird densities were six times greater when krill was abundant than when krill was scarce. Sooty shearwaters, which appeared to be moving through the area, were the only abundant bird species not correlated with krill abundance. Possible reasons why previous studies have not detected correlations between seabird and krill abundances are discussed.     

Response of the waterbird community to floating pennywort (Hydrocotyle ranunculoides) cover at Ngamo dam,Antelope Park,Zimbabwe.

Little is known on the influence of invasive aquatic weeds on afro‐tropical waterbird communities. We used bird counts in sites of varying floating pennywort Hydrocotyle ranunculoides cover to explore the relationship between the weed and the waterbird community dynamics at Ngamo dam, Antelope Park, Zimbabwe. Waterbird communities in low‐to‐medium weed cover sites were more diverse and abundant compared to sites of high weed cover. In addition to supporting birds such as African Jacana which are able to forage within dense aquatic plants, high weed cover sites were associated with birds whose diets include invertebrates and fish which are likely more abundant and diverse in these sites. In contrast, low‐to‐medium weed cover sites were associated with bird species such as Common Moorhen, Great Egret, Pied Kingfisher and African Fish Eagle which require accessible open water and forage for diving, swimming or wading. The increased bird abundance in low‐to‐medium weed cover sites also likely increases prey for predatory birds. Thus, the negative changes in the waterbird community composition, abundance and diversity in response to increasing floating pennywort cover reflects species‐specific tolerances to floating pennywort and its influence on accessible open water, foraging sites and prey availability.     

Fluorescent localization of thiols and disulfides in marsupial spermatozoa by bromobimane labelling

The acrosome of marsupial spermatozoa is a robust structure which, unlike its placental counterpart, resists disruption by detergent or freeze/thawing and does not undergo a calcium ionophore induced acrosome reaction. In this study specific fluorescent thiol labels, bromobimanes, were used to detect reactive thiols in the intact marsupial spermatozoon and examine whether disulfides play a role in the stability of the acrosome. Ejaculated brushtail possum (Trichosurus vulpecula) and tammar wallaby (Macropus eugenii) spermatozoa were washed by swim up and incubated with or without dithiothreitol (DTT) in order to reduce disulfides to reactive thiols. Spermatozoa were then washed by centrifugation and treated with monobromobimane (mBBr), a membranepermeable bromobimane, or with monobromotrimethylammoniobimane (qBBr), a membrane-impermeable bromobimane. Labelled spermatozoa were examined by fluorescence microscopy and sperm proteins (whole sperm proteins and basic nuclear proteins) were analysed by gel electrophoresis. The membrane-permeable agent mBBr lightly labelled the perimeter of the acrosome of non-DTT-treated possum and wallaby spermatozoa, indicating the presence of peri-acrosomal thiol groups. After reduction of sperm disulfides by DTT, mBBr labelled the entire acrosome of both species. The membrane-impermeable agent qBBr did not label any part of the acrosome in non-DTT or DTT-treated wallaby or possum spermatozoa. Thiols and disulfides are thus associated with the marsupial acrosome. They are not found on the overlying plasma membrane but are either in the acrosomal membranes and/or matrix. The sperm midpiece and tail were labelled by mBBr, with increased fluorescence observed in DTT-treated spermatozoa. The nucleus was not labelled in non-DTT or DTT-treated spermatozoa. Electrophoretic analysis confirmed the microscopic observations: Basic nuclear protein (protamines) lacked thiols or disulfide groups. Based on these findings, the stability of the marsupial acrosome may be due in part to disulfide stabilization of the acrosomal membranes and/or acrosomal matrix. In common with placental mammals, thiol and disulfide containing proteins appear to play a role in the stability of sperm tail structures. It was confirmed that the fragile marsupial sperm nucleus lacked thiols and disulfides. © 1994 Wiley-Liss, Inc.     

Patterns of bird abundance and habitat use in rice fields of the Kanto Plain, central Japan

Rice fields, the most widespread cropland in Japan, provide important habitat for birds, but few studies have examined bird communities that are influenced by agriculture in rice fields. I studied the seasonal pattern of bird abundance and their habitat preferences in terms of agricultural habitats in the rice fields of the central Kanto Plain, central Japan. In total, 19 waterbird species and 31 landbird species were recorded over the study year. Waterbird occurrence was largely restricted to the cultivation season (i.e. the flooded season, May–August), with maximum abundance in August. Rice fields dried up during winter and supported few waterbirds except for irregular occurrences of little egrets (Egretta garzetta) and common snipes (Gallinago gallinago), which selectively used ditches with shallow water. Landbird abundance increased after the start of cultivation (May) to August, but more birds were attracted to the rice fields in midwinter (around January). For both waterbirds and landbirds, most species preferred levees and fallow grass fields, but avoided fields with rice vegetation. Unplowed fields after harvest were preferred by several species including skylarks (Alauda arvensis), while plowed fields were less attractive to most species. These results suggest the need for advanced agricultural practices to include improvement in habitat quality for birds.     

Proteins of marsupial erythrocyte membranes

The proteins of erythrocyte membranes from the red kangaroo, western grey kangaroo, eastern grey wallaroo (euro), red-necked wallaby, Tammar wallaby, and brush-tail possum have been fractionated on acrylamide gels in the presence of sodium dodecyl sulfate. The pattern of proteins was remarkably similar between the different marsupial species. The pattern of Coomassie blue-staining proteins in the membranes of these species was also very similar to that of the human erythrocyte membrane. However, the glycoproteins in the marsupial erythrocyte membranes were markedly less conspicuous than those of the human erythrocyte membrane. Furthermore, the mobilities of the glycoproteins from the marsupials were different from those of the human erythrocyte membrane. The erythrocytes of the western grey kangaroo, the eastern wallaroo and the red-necked wallaby showed pronounced resistance to hypotonic lysis compared with those of the Tammar wallaby and the human. This effect seems to be related to the size of the erythrocytes rather than to differences in their protein composition.     

Resources at the landscape scale influence possum abundance

Abstract There is increasing realization that human perceptions of a ‘patchy’ environment do not necessarily relate to that of a particular organism and ecological characteristics of land adjacent to patches of natural vegetation (‘remnants’) may influence the ecological processes within. We investigated how the distribution and abundance of resources within and surrounding 39 small remnants located within the city of Melbourne, Australia, influenced the abundance of common brushtail possums Trichosurus vulpecula (Phalangeridae) and common ringtail possums Pseudocheirus peregrinus (Pseudocheiridae), two primarily folivorous marsupials. Bayesian modelling techniques were used to relate the abundance of both possum species to estimates of den and food availability both within the remnant and within a 100‐m buffer strip extending out into adjacent residential development. We found that both brushtail and ringtail possum abundance within remnants increased with the density of potential den sites within the remnant and food availability within the surrounding landscape. Ringtail possum density within the remnant further increased with den availability within the surrounding landscape. Our results lend support to the hypothesis that, in highly modified landscapes, processes originating outside a remnant vegetation patch may influence the abundance of species within.     

The relative importance of birds and insects as pollinators of the New Zealand flora

Native birds may have been underestimated as pollinators of the New Zealand flora due to their early decline in abundance and diversity on the mainland. This paper reconsiders the relative importance of birds and insects as pollinators to eight native flowering plants, representing a range of pollination syndromes, on two offshore island refuges. Experimental manipulations were made on five of these plant species to assess the relative effectiveness of bird and insect visitors as pollinators. In addition, foraging behaviour and the respective morphologies of flowers and visitors were measured at all eight plants to identify the main pollinators. The experimental measures showed that percentage fruit set was significantly higher in flowers exposed to birds than flowers from which birds were excluded in all manipulated plants. The observational measures revealed that for six of the flowering species (Sophora microphylla, Vitex lucens, Pittosporum crassifolium, Pittosporum umbellatum, Pseudopanax arboreus and Dysoxylum spectabile) the endemic honeyeaters were most likely to meet the conditions necessary for successful pollination. For the remaining two species (Metrosideros excelsa and Geniostoma ligus trifolium) the contribution by honeyeaters and insects to pollination was equivalent. The results suggest that the role of the endemic honeyeaters in pollination of the New Zealand flora, and the subsequent regeneration of native forest ecosystems, should be important considerations in ecosystem management.     

The density and stability of birds in shrubland and drainage line woodland in the southern Karoo,South Africa

Comparative surveys of bird species richness and abundance showed that a total of 69 bird species was recorded on 1 km transects (n = 996) through shrubland, with a total estimated density of birds of 32.7 ± 32.8 (S.D.) birds/km², of which resident birds made up 24.4 ± 21.0 birds/km², nomads made up 1.9 ± 11.7 birds/km² and local nomads made up 6.3 ± 20.1 birds/km². There was no correlation in general between total bird numbers or total bird biomass and rain, but numbers and biomass of nomads showed the strongest correlations with rainfall two months previously. A total of 86 species was recorded on 276 surveys along a 1 km stretch of drainage line woodland. Species richness varied from an average of about nine species to 24 species each month, and only showed a marked change during the 'dry' period of 1990 to early 1991. Density of birds in this woodland was 59.2 ± 20.8 birds/km. Biomass of birds was relatively constant throughout the study period, with marked increases in certain months when large species were present.     

Responses of kukupa (

The kukupa or New Zealand pigeon (Hemiphaga novaeseelandiae) is gradually declining on the New Zealand mainland, due mostly to predation by introduced pest mammals including ship rats (Rattus rattus) and brushtail possums (Trichosurus vulpecula). We report on a co-operative project between Maori landowners, the Department of Conservation, and Manaaki Whenua–Landcare Research researchers to restore a Northland kukupa population and to examine kukupa nesting success in relation to pest abundance. Ship rats and possums were targeted by trapping and poisoning throughout Motatau Forest (350 ha) from 1997 to 1999; only possums were targeted in 2000. All 13 kukupa nests located before pest control started in late 1997 failed at the egg stage, but all seven nests located in 1998–99 successfully fledged young when trapping and tracking indices of possums and ship rats were less than 4%. After pest control, counts of kukupa and some other bird species increased at Motatau compared with counts in a nearby non-treatment block, suggesting numbers of adult kukupa can be increased in small forest areas by intensive pest control. This increase is due at least partly to increased nest success. Evidence from time-lapse video cameras, sign remaining at nests, and nest success rates under different pest control regimes suggest both ship rats and possums are important predators at kukupa nests.     

Avian species diversity in different habitat types in and around North Nandi Forest,Kenya

Forest fragmentation and degradation leads to formation of modified habitats whose ability to support existing avifaunal diversity is still largely unknown. Bird diversity in indigenous forest, disturbed forest, plantation forest and farmlands adjacent to North Nandi Forest reserve was studied between January 2015 and June 2015. The distribution of bird feeding guilds in these habitat patches was also evaluated. Birds were surveyed using point counts, timed species counts and line transects and classified into six feeding guilds. A total of 3,232 individual birds of 151 species were recorded in the four habitats. Significant difference on bird abundance across the four habitats (F = 15.141, P ≤ 0.05, df = 3, 1121) was noted. Shannon–Weiner diversity index H′ for bird community ranged from 3.06 for plantation forest to 4.05 for disturbed forest showing a relatively diverse bird community. Insectivores (F = 3.090, P ≤ 0.05, df = 3, 297) dominated the foraging species assemblage in all the habitats significantly. Linear regression analysis revealed a strong linear relationship on bird species richness and abundance with vegetation variables (P < 0.01 in all cases). The results indicate that disturbed forest and indigenous forest support high bird species richness than plantation forest and farmlands. However, high bird abundance was observed in farmlands and plantation forest as opposed to indigenous forest and disturbed forest as they provide dispersal routes over a short distance and are important for creating corridors between primary forests.     

Isolation and characterization of marsupial IL5 genes

The genomic nucleotide sequence and chromosomal position of the interleukin 5 (IL5) gene has been described for the model marsupial Macropus eugenii (tammar wallaby). A 272 base pair genomic IL5 polymerase chain reaction (PCR) product spanning exon 3, intron 3, and exon 4 was generated using stripe-faced dunnart (Sminthopsis macroura) DNA. This PCR product was used to isolate a genomic lambda clone containing the complete IL5 gene from a tammar wallaby EMBL3 lambda library. Sequencing revealed that the tammar wallaby IL5 gene consists of four exons separated by three introns. Comparison of the marsupial coding sequence with coding sequences from eutherian species revealed 61 to 69% identity at the nucleotide level and 48 to 63% identity at the amino acid (aa) level. A polymorphic complex compound microsatellite was identified within intron 2 of the tammar wallaby IL5 gene. This microsatellite was also found in other marsupials including the swamp wallaby, tree kangaroo, stripe-faced dunnart, South American opossum, brushtail possum, and koala. Fluorescence in situ hybridization using DNA from the IL5 clone on tammar wallaby chromosomes indicated that the IL5 gene is located on Chromosome 1.