共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
Stimulation of RNA synthesis at initiation by ribosomal proteins 总被引:2,自引:0,他引:2
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
RNA aptamers selected against DNA polymerase beta inhibit the polymerase activities of DNA polymerases beta and kappa 下载免费PDF全文
Gening LV Klincheva SA Reshetnjak A Grollman AP Miller H 《Nucleic acids research》2006,34(9):2579-2586
DNA polymerase β (polβ), a member of the X family of DNA polymerases, is the major polymerase in the base excision repair pathway. Using in vitro selection, we obtained RNA aptamers for polβ from a variable pool of 8 × 1012 individual RNA sequences containing 30 random nucleotides. A total of 60 individual clones selected after seven rounds were screened for the ability to inhibit polβ activity. All of the inhibitory aptamers analyzed have a predicted tri-lobed structure. Gel mobility shift assays demonstrate that the aptamers can displace the DNA substrate from the polβ active site. Inhibition by the aptamers is not polymerase specific; inhibitors of polβ also inhibited DNA polymerase κ, a Y-family DNA polymerase. However, the RNA aptamers did not inhibit the Klenow fragment of DNA polymerase I and only had a minor effect on RB69 DNA polymerase activity. Polβ and κ, despite sharing little sequence similarity and belonging to different DNA polymerase families, have similarly open active sites and relatively few interactions with their DNA substrates. This may allow the aptamers to bind and inhibit polymerase activity. RNA aptamers with inhibitory properties may be useful in modulating DNA polymerase actvity in cells. 相似文献
15.
16.
17.
18.
Transcription of chromatin by human RNA polymerase 总被引:3,自引:0,他引:3
19.