Life cycle assessment of copper production: a case study in China

Purpose

China is the world’s largest producer and consumer of refined and reclaimed copper because of the rapid economic and industrial development of this country. However, only a few studies have analyzed the environmental impact of China’s copper industry. The current study analyzes the life cycle environmental impact of copper production in China.

Methods

A life cycle impact assessment using the ReCiPe method was conducted to estimate the environmental impact of refined and reclaimed copper production in China. Uncertainty analysis was also performed based on the Monte-Carlo simulation.

Results and discussion

The environmental impact of refined copper was higher than that of reclaimed copper in almost all categories except for human toxicity because of the direct atmospheric arsenic emission during the copper recycling stage. The overall environmental impact for the refined copper production was mainly attributed to metal depletion, freshwater ecotoxicity, marine ecotoxicity, and water depletion potential impact. By contrast, that for the reclaimed copper production was mainly caused by human toxicity impact.

Conclusions

Results show that the reclaimed copper scenario had approximately 59 to 99% more environmental benefits than those of the refined copper scenario in most key categories except for human toxicity, in which a similar environmental burden was observed between both scenarios. The key factors that reduce the overall environmental impact for China’s copper industry include decreasing direct heavy metal emissions in air and water, increasing the national recycling rate of copper, improving electricity consumption efficiency, replacing coal with clean energy sources for electricity production, and optimizing the efficiency of copper ore mining and consumption.

     

Metabolomic profiling of maternal hair suggests rapid development of intrahepatic cholestasis of pregnancy

Introduction

Intrahepatic cholestasis of pregnancy (ICP) is a common maternal liver disease; development can result in devastating consequences, including sudden fetal death and stillbirth. Currently, recognition of ICP only occurs following onset of clinical symptoms.

Objective

Investigate the maternal hair metabolome for predictive biomarkers of ICP.

Methods

The maternal hair metabolome (gestational age of sampling between 17 and 41 weeks) of 38 Chinese women with ICP and 46 pregnant controls was analysed using gas chromatography–mass spectrometry.

Results

Of 105 metabolites detected in hair, none were significantly associated with ICP.

Conclusion

Hair samples represent accumulative environmental exposure over time. Samples collected at the onset of ICP did not reveal any metabolic shifts, suggesting rapid development of the disease.

     

Life cycle assessment and life cycle costing of road infrastructure in residential neighbourhoods

Purpose

The built environment consists of a huge amount of infrastructure, such as roads and utilities. The objective of this paper is to assess the life cycle financial and environmental impact of road infrastructure in residential neighbourhoods and to analyse the relative contribution of road infrastructure in the total impact of neighbourhoods.

Methods

Various road sections are analysed based on an integrated life cycle approach, combining life cycle costing and life cycle assessment. To deal with complexity, a hierarchic assessment structure, using the principles of the “element method for cost control”, is implemented. Four neighbourhood models with diverse built densities are compared to gain insight in the relative impact of road infrastructure in neighbourhoods.

Results and discussion

The results reveal important financial and environmental impact differences between the road sections analysed. Main contributors to the life cycle financial and environmental impact are the surface layer and electrical and piped services. The contribution of road infrastructure to the total neighbourhood impact, ranging from 2 to 9 % of the total cost, is relatively limited, compared to buildings, but not negligible in low built density neighbourhoods.

Conclusions

Good spatial planning of the neighbourhood is recommended to reduce the amount of road infrastructure and the related financial and environmental impact. The priority should be to design denser neighbourhood layouts, before decreasing the financial and environmental impact of the road sections.

     

An effective hybrid of hill climbing and genetic algorithm for 2D triangular protein structure prediction

Background

Proteins play fundamental and crucial roles in nearly all biological processes, such as, enzymatic catalysis, signaling transduction, DNA and RNA synthesis, and embryonic development. It has been a long-standing goal in molecular biology to predict the tertiary structure of a protein from its primary amino acid sequence. From visual comparison, it was found that a 2D triangular lattice model can give a better structure modeling and prediction for proteins with short primary amino acid sequences.

Methods

This paper proposes a hybrid of hill-climbing and genetic algorithm (HHGA) based on elite-based reproduction strategy for protein structure prediction on the 2D triangular lattice.

Results

The simulation results show that the proposed HHGA can successfully deal with the protein structure prediction problems. Specifically, HHGA significantly outperforms conventional genetic algorithms and is comparable to the state-of-the-art method in terms of free energy.

Conclusions

Thanks to the enhancement of local search on the global search, the proposed HHGA achieves promising results on the 2D triangular protein structure prediction problem. The satisfactory simulation results demonstrate the effectiveness of the proposed HHGA and the utility of the 2D triangular lattice model for protein structure prediction.

     

LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels

Introduction

Metabolome analysis is complicated by the continuous dynamic changes of metabolites in vivo and ex vivo. One of the main challenges in metabolomics is the robustness and reproducibility of results, partially driven by pre-analytical variations.

Objectives

The objective of this study was to analyse the impact of pre-centrifugation time and temperature, and to determine a quality control marker in plasma samples.

Methods

Plasma metabolites were measured by gas chromatography-mass spectrometry (GC–MS) and analysed with the MetaboliteDetector software. The metabolites, which were the most labile to pre-analytical variations, were further measured by enzymatic assays. A score was calculated for their use as quality control markers.

Results

The pre-centrifugation temperature was shown to be critical in the stability of plasma samples and had a significant impact on metabolite concentration profiles. In contrast, pre-centrifugation delay had only a minor impact. Based on the results of this study, whole blood should be kept on wet ice and centrifuged within maximum 3 h as a prerequisite for preparing EDTA plasma samples fit for the purpose of metabolome analysis.

Conclusions

We have established a novel blood sample quality control marker, the LacaScore, based on the ascorbic acid to lactic acid ratio in plasma, which can be used as an indicator of the blood pre-centrifugation conditions, and hence the suitability of the sample for metabolome analyses. This method can be applied in research institutes and biobanks, enabling assessment of the quality of their plasma sample collections.

     

Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics

Introduction

Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites.

Objectives

In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting.

Methods

The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces.

Results

A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed.

Conclusion

The workflow generated repeatable and informative fingerprints for robust metabolome characterization.

     

Regioselective C-H hydroxylation of omeprazole sulfide by <Emphasis Type="Italic">Bacillus megaterium</Emphasis> CYP102A1 to produce a human metabolite

Objectives

To find a simple enzymatic strategy for the efficient synthesis of the expensive 5′-hydroxyomeprazole sulfide, a recently identified minor human metabolite, from omeprazole sulfide, which is an inexpensive substrate.

Results

The practical synthetic strategy for the 5′-OH omeprazole sulfide was accomplished with a set of highly active CYP102A1 mutants, which were obtained by blue colony screening from CYP102A1 libraries with a high conversion yield. The mutant and even the wild-type enzyme of CYP102A1 catalyzed the high regioselective (98 %) C-H hydroxylation of omeprazole sulfide to 5′-OH omeprazole sulfide with a high conversion yield (85–90 %).

Conclusions

A highly efficient synthesis of 5′-OH omeprazole sulfide was developed using CYP102A1 from Bacillus megaterium as a biocatalyst.

     

A modeling framework to evaluate sustainability of building construction based on LCSA

Purpose

Life cycle sustainability assessment (LCSA) is a method that combines three life cycle techniques, viz. environmental life cycle assessment (LCA), life cycle costing (LCC), and social life cycle assessment (S-LCA). This study is intended to develop a LCSA framework and a case study of LCSA for building construction projects.

Methods

A LCSA framework is proposed to combine the three life cycle techniques. In the modeling phases, three life cycle models are used in the LCSA framework, namely the environmental model of construction (EMoC), cost model of construction (CMoC), and social-impact model of construction (SMoC). A residential building project is applied to the proposed LCSA framework from “cradle to the end of construction” processes to unveil the limitations and future research needs of the LCSA framework.

Results and discussion

It is found that material extraction and manufacturing account for over 90 % to the environmental impacts while they contribute to 61 % to the construction cost. In terms of social impacts, on-site construction performs better than material extraction and manufacturing, and on-site construction has larger contributions to the positive social impacts. The model outcomes are validated through interviews with local experts in Hong Kong. The result indicates that the performance of the models is generally satisfactory.

Conclusions

The case study has confirmed that LCSA is feasible. Being one of the first applications of LCSA on building construction, this study fulfills the current research gap and paves the way for future development of LCSA.

     

Genetic variation in the TAS2R38 taste receptor contributes to the oral microbiota in North and South European locations: a pilot study

Background

Microbial communities are influenced by environmental factors including host genetics. We investigated the relationship between host bitter taste receptor genotype hTAS2R38 and oral microbiota, together with the influence of geographical location.

Methods

hTAS2R38 polymorphisms and 16S bacterial gene sequencing from oral samples were analyzed from a total of 45 healthy volunteers from different geographical locations.

Results

Genetic variation in the bitter taste receptor TAS2R38 reflected in the microbial composition of oral mucosa in Finnish and Spanish subjects. Multivariate analysis showed significant differences in the microbial composition between country and also dependent on taste genotype. Oral microbiota was shown to be more stable to the geographical location impact among AVI-homozygotes than PAV-homozygotes or heterozygotes (PAV/AVI).

Conclusion

Geographical location and genetic variation in the hTAS2R38 taste receptor impact oral mucosa microbial composition. These findings provide an advance in the knowledge regarding the interactions between taste receptor genes and oral microbiota. This study suggests the role of host-microbiota interactions on the food taste perception in food choices, nutrition, and eating behavior.

     

Life cycle assessment of the construction of an unpaved road in an undisturbed tropical rainforest area in the vicinity of Manu National Park,Peru

Purpose

The main goal of this study is to provide a thorough environmental sustainability analysis of the construction, traffic, and maintenance of a 45.6-km section of the ‘Manu Road’, an unpaved tropical road that is currently being built in the vicinity of Manu National Park, in the region of Madre de Dios, Peru.

Methods

Life cycle assessment (LCA) using a set of 18 different impact categories was selected to conduct the environmental analysis. Modelling of machinery and vehicle emissions, as well as dust emissions, was performed to account for site-specific characteristics in terms of road construction and traffic. Similarly, direct land use changes were modelled with a particular emphasis on the decay of deforested biomass during construction. A set of different scenarios for the production system were considered to account for uncertainty regarding vehicle transit, amount of deforested biomass, and emission standards.

Results and discussion

Construction, maintenance, and traffic of the Manu Road varied considerably depending on methodological assumptions. Deforestation due to direct land use changes appears to be the main environmental hotspot in terms of climate change, whereas in the remaining impact categories, traffic was the main carrier of environmental burdens.

Conclusions

To the best of our knowledge, this study is the first LCA that focuses on the construction, maintenance, and traffic in a tropical rainforest environment. Despite the low requirements in terms of materials and technology to build this road, its derived environmental impacts are relevant in terms of climate change and particulate matter formation due to deforestation and dust emissions, respectively. Unpaved roads represent a relevant proportion of the entire road network worldwide, especially in developing tropical countries, playing a crucial role in the transportation of raw materials. Furthermore, road infrastructure is expected to expand explosively in the decades to come. Therefore, we suggest that LCA studies can and should improve the planning of road infrastructure in terms of life cycle inventories.

     

LCA applied to nano scale zero valent iron synthesis

Purpose

Application of zero valent iron nanoparticles is an innovative technology for ground water treatment and soil remediation. There are several methods to synthesise nano scale zero valent iron (nZVI), namely through bottom-up methods which consists on chemical reactions using strong reducing agents. In this work, the environmental impacts and costs were determined for two methods, namely the traditional one that uses sodium borohydride and the green method that uses extracts obtained from natural products.

Methods

The consideration of environment and economic aspects in the earlier stages of the synthesis processes and in the development of new materials is of great importance since it can help to decide if alternative methods are promising and should be further developed aiming more sustainable processes. In this work, life cycle assessment (LCA) was used as an ecodesign strategy evaluating environmental performance of the two synthesis methods, identifying critical stages of the synthesis processes of nZVI. An economic evaluation and a sensitivity analysis considering a different scenario for electricity production were also performed.

Results and discussion

The results obtained in this study showed that the green synthesis method presents lower environmental impacts than the traditional one, roughly 50% lower in the first scenario. In the second scenario, environmental impact of green synthesis corresponds to 38% of the environmental impact of traditional synthesis. In the green method, the critical stage is the extraction process which is closely related to the electricity production. In the traditional method, the reactant use is the critical stage that is related to the production of sodium borohydride. The economic evaluation indicated that the traditional synthesis method is much more expensive than the green synthesis (roughly eight times higher).

Conclusions

From the results obtained, it is possible to conclude that the green synthesis method presents lower environmental impacts in both scenarios and lower costs than the traditional synthesis.

     

A review of environmental life cycle assessment studies examining cheese production

Purpose

Cheese is one of the world’s most widely consumed dairy products and its popularity is ever growing. However, as concerns for the environmental impact of industries increase, products like cheese, which have a significant environmental impact, may lose their popularity. A commonly used technique to assess the environmental impact of a product is life cycle assessment (LCA). In this paper, a state-of-the-art review of LCA studies on the environmental impact of cheese production is presented.

Methods

Sixteen LCA studies, which explored the impact from the production of a variety of cheese types (fresh, mature and semi-hard) were examined and discussed. The four stages of the LCA were examined and the range of results of selected environmental impact categories (global warming potential, acidification potential and eutrophication potential) were detailed and discussed.

Results and discussion

For each of these environmental impact categories, raw milk production was consistently found to be the most significant contributor to the total impact, which was followed by processing. It was found that allocation between cheese and its by-products was crucial in determining the impact of cheese production and standardisation or guidelines may be needed. Very little information relating to wastewater treatment system and processes were reported and this leads to inaccurate environmental impact modelling relating to these aspects of the manufacture of cheese. Very few studies included the design of packaging in terms of reducing food waste, which may significantly contribute to the overall environmental impact.

Conclusions

As raw milk production was found to have the greatest contribution to environmental impact, mitigation strategies at farm-level, particularly in relation to enteric fermentation and manure management, need to be implemented. Additionally, based on the literature, there is a suggestion that fresh cheese has less of an environmental impact than semi-hard cheeses, particularly when examining direct energy consumption. However, there needs to be more case studies investigated to justify this statement.

     

Do green wooden composites using lignin-based binder have environmentally benign alternatives? A preliminary LCA case study in China

Purpose

Nowadays, formaldehyde emissions from petroleum-based adhesives contribute considerably to environmental problems and are a constraint to the development of forest-based industries. Although many efforts are being made to develop new lignin-based adhesives for panels, very few studies were carried out via life cycle assessment (LCA). This study aims to assess the life cycle of green wooden composites by using hybrid-modified ammonium lignosulfonate (HMAL) as the binder and investigate the possibility of lignin-based binder to be a good alternative.

Methods

This study is a step further of the previous work conducted on HMAL as an alternative binder for medium-density fiberboard (MDF) or, in other words, the wooden composite made from HMAL and wood fiber (WF). LCA was carried out to assess the environmental impacts during the life cycle of the new manufacturing process of HMAL/WF production using ReCiPe 1.08 Endpoint and IPCC global warming potential (GWP) method built into the GaBi version 6.0 software. The production system involved two subsystems: raw material supply and board manufacture. Meanwhile, a comparative LCA of conventional MDF, with three main damage categories and GWP, was also carried out.

Results and discussion

The hydrogen peroxide (H₂O₂) production, electricity, and the HMAL/WF manufacturing stages had the greatest environmental impact. The comparative results pointed out that HMAL/WF production is environmentally superior to conventional MDF in general. Due to the environmental impacts associated with the HMAL binder, a sensitivity analysis was carried out. Suggestions were made for a cleaner production, in which the H₂O₂ dose was reduced to 24 wt%.

Conclusions

H₂O₂ use, energy, and electricity consumption are main contributors to most impact categories, which help us to find the potential improvements of sustainability, choose the appropriate HMAL technology, and optimize the HMAL/WF system. Feasible production processes and life cycle costs are factors that still need to be studied.

     

Effect of experimental gingivitis induction and erythritol on the salivary metabolome and functional biochemistry of systemically healthy young adults

Introduction

Understanding the changes occurring in the oral ecosystem during development of gingivitis could help improve prevention and treatment strategies for oral health. Erythritol is a non-caloric polyol proposed to have beneficial effects on oral health.

Objectives

To examine the effect of experimental gingivitis and the effect of erythritol on the salivary metabolome and salivary functional biochemistry.

Methods

In a two-week experimental gingivitis challenge intervention study, non-targeted, mass spectrometry-based metabolomic profiling was performed on saliva samples from 61 healthy adults, collected at five time-points. The effect of erythritol was studied in a randomized, controlled trial setting. Fourteen salivary biochemistry variables were measured with antibody- or enzymatic activity-based assays.

Results

Bacterial amino acid catabolites (cadaverine, N-acetylcadaverine, and α-hydroxyisovalerate) and end-products of bacterial alkali-producing pathways (N-α-acetylornithine and γ-aminobutyrate) increased significantly during the experimental gingivitis. Significant changes were found in a set of 13 salivary metabolite ratios composed of host cell membrane lipids involved in cell signaling, host responses to bacteria, and defense against free radicals. An increase in mevalonate was also observed. There were no significant effects of erythritol. No significant changes were found in functional salivary biochemistry.

Conclusions

The findings underline a dynamic interaction between the host and the oral microbial biofilm during an experimental induction of gingivitis.

     

Pseudo current density maps of electrophysiological heart,nerve or brain function and their physical basis

Background

In recent years the visualization of biomagnetic measurement data by so-called pseudo current density maps or Hosaka-Cohen (HC) transformations became popular.

Methods

The physical basis of these intuitive maps is clarified by means of analytically solvable problems.

Results

Examples in magnetocardiography, magnetoencephalography and magnetoneurography demonstrate the usefulness of this method.

Conclusion

Hardware realizations of the HC-transformation and some similar transformations are discussed which could advantageously support cross-platform comparability of biomagnetic measurements.

     

Comparative life cycle assessment and life cycle costing of lodging in the Himalaya

Purpose

The main aim of the study is to assess the environmental and economic impacts of the lodging sector located in the Himalayan region of Nepal, from a life cycle perspective. The assessment should support decision making in technology and material selection for minimal environmental and economic burden in future construction projects.

Methods

The study consists of the life cycle assessment and life cycle costing of lodging in three building types: traditional, semi-modern and modern. The life cycle stages under analysis include raw material acquisition, manufacturing, construction, use, maintenance and material replacement. The study includes a sensitivity analysis focusing on the lifespan of buildings, occupancy rate and discount and inflation rates. The functional unit was formulated as the ‘Lodging of one additional guest per night’, and the time horizon is 50 years of building lifespan. Both primary and secondary data were used in the life cycle inventory.

Results and discussion

The modern building has the highest global warming potential (kg CO_2-eq) as well as higher costs over 50 years of building lifespan. The results show that the use stage is responsible for the largest share of environmental impacts and costs, which are related to energy use for different household activities. The use of commercial materials in the modern building, which have to be transported mostly from the capital in the buildings, makes the higher GWP in the construction and replacement stages. Furthermore, a breakdown of the building components shows that the roof and wall of the building are the largest contributors to the production-related environmental impact.

Conclusions

The findings suggest that the main improvement opportunities in the lodging sector lie in the reduction of impacts on the use stage and in the choice of materials for wall and roof.

     

A lost opportunity for science: journals promote data sharing in metabolomics but do not enforce it

Introduction

Data sharing is being increasingly required by journals and has been heralded as a solution to the ‘replication crisis’.

Objectives

(i) Review data sharing policies of journals publishing the most metabolomics papers associated with open data and (ii) compare these journals’ policies to those that publish the most metabolomics papers.

Methods

A PubMed search was used to identify metabolomics papers. Metabolomics data repositories were manually searched for linked publications.

Results

Journals that support data sharing are not necessarily those with the most papers associated to open metabolomics data.

Conclusion

Further efforts are required to improve data sharing in metabolomics.

     

Blue mussel (<Emphasis Type="Italic">Mytilus edulis</Emphasis>) bouchot culture in Mont-St Michel Bay: potential mitigation effects on climate change and eutrophication

Purpose

Bivalve production is an important aquaculture activity worldwide, but few environmental assessments have focused on it. In particular, bivalves’ ability to extract nutrients from the environment by intensely filtering water and producing a shell must be considered in the environmental assessment.

Methods

LCA of blue mussel bouchot culture (grown out on wood pilings) in Mont Saint-Michel Bay (France) was performed to identify its impact hotspots. The chemical composition of mussel flesh and shell was analyzed to accurately identify potential positive effects on eutrophication and climate change. The fate of mussel shells after consumption was also considered.

Results and discussion

Its potential as a carbon-sink is influenced by assumptions made about the carbon sequestration in wooden bouchots and in the mussel shell. The fate of the shells which depends on management of discarded mussels and household waste plays also an important role. Its carbon-sink potential barely compensates the climate change impact induced by the use of fuel used for on-site transportation. The export of N and P in mussel flesh slightly decreases potential eutrophication. Environmental impacts of blue mussel culture are determined by the location of production and mussel yields, which are influenced by marine currents and the distance to on-shore technical base.

Conclusions

Bouchot mussel culture has low environmental impacts compared to livestock systems, but the overall environmental performances depend on farming practices and the amount of fuel used. Changes to the surrounding ecosystem induced by high mussel density must be considered in future LCA studies.

     

Untargeted metabolomics suffers from incomplete raw data processing

Introduction

Untargeted metabolomics is a powerful tool for biological discoveries. To analyze the complex raw data, significant advances in computational approaches have been made, yet it is not clear how exhaustive and reliable the data analysis results are.

Objectives

Assessment of the quality of raw data processing in untargeted metabolomics.

Methods

Five published untargeted metabolomics studies, were reanalyzed.

Results

Omissions of at least 50 relevant compounds from the original results as well as examples of representative mistakes were reported for each study.

Conclusion

Incomplete raw data processing shows unexplored potential of current and legacy data.