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1.
通过测定块根老鹳草中总鞣质的含量,分析块根老鹳草中总鞣质的积累动态规律。方法:采用紫外分光光度法测定。结果:块根老鹳草叶中鞣质含量是花期最高,茎的鞣质含量普遍偏低;地上部分药材的鞣质含量是始果期含量最高。结论:建立了稳定、可靠的鞣质含量测定方法,块根老鹳草中鞣质的含量变化规律对于确定药材的药用部位及最佳采收期具有指导意义。 相似文献
2.
Karin Readel David Seigler Kuo Hwang June Keesy Susan Seilheimer 《Economic botany》2001,55(2):212-222
The percentage of tannins in leaves, bark, wood, and immature fruits of several species of Acacia and related mimosoid legumes
from the southwestern U.S. and Mexico, along with a few from Costa Rica and Argentina, was determined by a modified hide powder
procedure and by precipitation with casein. The relative percentages of hydrolyzable and condensed tannins were determined
by the iodate and the vanillin-HCl methods, respectively. Gallotannins of selected samples were also determined by the rhodanine
method. Although the amount of total tannins was similar for the first two methods, values for condensed tannins by the vanillin-HCl
method were frequently two to four times greater than the total tannin values. 相似文献
3.
Summary The concept of protein precipitation potential has recently been introduced by Wisdom et al. (1987) as a means to combine chemical and protein precipitation assays of tannins for ecological studies. The definition of protein precipitation potential was not theoretically rigorous, and data analysis was obscure. Our attempts to repeat the tannin extraction procedure gave incomplete recovery (24% loss of quebracho) of condensed tannins, the only type considered by Wisdom et al. In contrast we found their method efficient for hydrolysable tannins (80% recovery of tannic acid) which were undetected by their chemical assay of tannins. Their protein precipitation assay was confounded by chemical interference from both types of tannins. We conclude with recommendations for this type of analysis. 相似文献
4.
Summary A series of seventeen plant extracts rich in phenolic materials, including condensed and hydrolysable tannins, have been subjected to a series of chemical analyses in an attempt to gather ecologically significant information about their structure. Procedures investigated were (i) the Folin-Denis and Hagerman and Butler methods for quantifying total phenolics, (ii) the vanillin and proanthocyanidin methods for quantifying condensed tannins, (iii) the iodate and nitrous acid methods for hydrolysable tannins. It was found that the techniques for total phenolics correlated well, the Hagerman and Butler method giving higher estimates where solutions were particularly phenol rich. By contrast there was considerable discrepancy between the methods examined for condensed tannins. This is probably due primarily to the very different chemical reactions that form the basis of these procedures and also to the fact that the extract dependent products of the proanthocyanidin method vary in their A
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values. During the study of condensed tannins methods for estimating their contribution to total phenolics and for measuring their average polymer length were examined. In both cases different procedures produced very variable results. Available methods for hydrolysable tannins were found not to be generally applicable across all extracts thought to contain this type of tannin on the basis of chromatographic analysis. An attempt to produce a quantitative spectrophotometric assay for hydrolysable tannins based on changes in reactivity to ferric chloride due to hydrolysis is described. This proved to be of limited sensitivity but may have some merit for estimating levels in hydrolysable tannins in phenol-rich plant extracts that also contain condensed tannins. It is concluded that whilst the overall level of phenolics in extracts can be estimated with some confidence the information imparted by more specific assays is very dependent on the procedures employed, particularly when dealing with extracts from taxonomically highly diverse sources. 相似文献
5.
Aguilera-Carbo A Augur C Prado-Barragan LA Favela-Torres E Aguilar CN 《Applied microbiology and biotechnology》2008,78(2):189-199
In the last years, tannin biodegradation has been the subject of a lot of studies due to its commercial importance and scientific
relevance. Tannins are molecules of low biodegradation and represent the main chemical group of natural anti-microbials occurring
in the plants. Among the different kinds of tannins, ellagitannins represent the group less studied manly due to their diversity
and chemical complexity. The general outline of this work includes information on tannins, their classification and properties,
biodegradation, ellagic acid production, and potential applications. In addition, it describes molecular, catalytic, and functional
information. Special attention has been focused on the biodegradation of ellagitannins describing the possible role of microbial
enzymes in the production of ellagic acid. 相似文献
6.
测定了野生地木耳(Nostoc commune Vauch.)中次生代谢产物含量,并与4种常见食用菌藻黑木耳(Auricularia auricular-judae)、银耳(Tremella fuciformis)、紫菜(Porphyra)、海带(Laminaria japonica)进行比较分析,总酚、总黄酮和缩合单宁含量测定采用分光光度法,总生物碱含量测定采用高效液相色谱法(HPLC)。结果显示,野生地木耳中总酚含量为24.255 mg/g±1.631 mg/g,总黄酮含量为5.741 mg/g±0.239 mg/g,总生物碱含量为0.768 mg/g±0.073 mg/g,缩合单宁含量为0.069 mg/g±0.009 mg/g。4种食用菌藻中次生代谢产物含量范围为:总酚(5.520~62.326 mg/g)、总黄酮(0.847~7.010 mg/g)、总生物碱(0.408~4.132 mg/g)、缩合单宁(0.063~0.233 mg/g);比较分析结果显示,野生地木耳次生代谢产物中的总酚和总黄酮物质含量较高,且总酚是主要次生代谢产物,缩合单宁与总生物碱含量较低;总酚含量约为黑木耳和银耳的2倍、紫菜的6倍;总黄酮含量约为黑木耳的7倍,银耳的3倍。 相似文献
7.
《Animal Feed Science and Technology》2006,125(1-2):139-149
Leaf samples of Juglans regia (walnut), Rhus typhina (sumach), Aesculus hippocastanum (chestnut) and Salix alba (willow) were either freeze dried or oven dried and the amount of tannins was determined by two chemical assays (Folin Ciocalteau and ferric chloride method). In addition, the biological activity of these tannins was determined by their protein precipitation capacity and by a bioassay based on the incubation to the leaves in vitro in buffered rumen fluid with and without polyethylene glycol. Generally, oven drying is recognised to decrease the extractability and/or the biological activity of tannins. Our results however do not indicate a general decrease in extractability of tannins due to oven drying. The effects observed were plant specific with negative effects of oven drying in walnut and willow leafs, positive effects in chestnut leaves and no effects were observed for the sumach. No negative effects of oven drying were detected for the biological activity of the tannins. The specific protein precipitation capacity was nearly identical for the freeze dried and the oven dried tannins. Oven drying however decreased the solubility of the cell contents and the plant cell wall, which led to changes in fermentation end products, but the biological activity of the tannins was not negatively affected by the oven drying. Although some differences in the extractability and activity of the individual plant species were observed, we conclude from this work that the drying procedure has no negative effect on the biological activity of the tannins examined. 相似文献
8.
油松为我国特有树种,药用历史悠久且分布广泛,其药用部位含有多酚和单宁等生物活性物质。本文采用Folin-Cicalten法和柠檬酸铁铵法对油松不同部位多酚与单宁含量进行了测定。实验结果表明,油松根、茎和叶多酚含量依次为89.24±12.06、68.18±11.20、104.30±12.51 mg·g-1;单宁含量依次为25.38±5.85、20.92±3.55、39.02±5.68 mg·g-1。油松多酚与单宁在油松各部位有相同的分布趋势,均为叶中最高,根次之,茎中最低。与其他中草药植物多酚含量相比,油松多酚含量较高,具有较大的开发前景和应用价值。 相似文献
9.
Chikako Kuwabara Donghui WangKeita Endoh Yukiharu FukushiKeita Arakawa Seizo Fujikawa 《Cryobiology》2013
Based on the discovery of novel supercooling-promoting hydrolyzable gallotannins from deep supercooling xylem parenchyma cells (XPCs) in Katsura tree (see Wang et al. (2012) [38]), supercooling capability of a wide variety of tannin-related polyphenols (TRPs) was examined in order to find more effective supercooling-promoting substances for their applications. The TRPs examined were single compounds including six kinds of hydrolyzable tannins, 11 kinds of catechin derivatives, two kinds of structural analogs of catechin and six kinds of phenolcarboxylic acid derivatives, 11 kinds of polyphenol mixtures and five kinds of crude plant tannin extracts. The effects of these TRPs on freezing were examined by droplet freezing assays using various solutions containing different kinds of identified ice nucleators such as the ice nucleation bacterium (INB) Erwinia ananas, the INB Xanthomonas campestris, silver iodide and phloroglucinol as well as a solution containing only unintentionally included unidentified airborne ice nucleators. Among the 41 kinds of TRPs examined, all of the hydrolyzable tannins, catechin derivatives, polyphenol mixtures and crude plant tannin extracts as well as a few structural analogs of catechin and phenolcarboxylic acid derivatives exhibited supercooling-promoting activity (SCA) with significant differences (p > 0.05) from at least one of the solutions containing different kinds of ice nucleators. It should be noted that there were no TRPs exhibiting ice nucleation-enhancing activity (INA) in all solutions containing identified ice nucleators, whereas there were many TRPs exhibiting INA with significant differences in solutions containing unidentified ice nucleators alone. An emulsion freezing assay confirmed that these TRPs did not essentially affect homogeneous ice nucleation temperatures. It is thought that not only SCA but also INA in the TRPs are produced by interactions with heterogeneous ice nucleators, not by direct interaction with water molecules. In the present study, several TRPs that might be useful for applications due to their high SCA in many solutions were identified. 相似文献
10.
《Journal of Fermentation and Bioengineering》1990,69(3):148-153
The effectiveness of several low molecular weight (MW) tannin measurement methods for indicating the tannin toxicity to methan bacteria was evaluated. The methanogenic toxicity of the low and high MW tannins from autoxidized bark extracts was studied by selective removal of MW fractions from the extract with active carbon adsorption and calcium precipitation treatments. The toxicity of the low MW tannin fraction and the nontoxicity of the high MW tannin fraction were demonstrated. The low MW tannin concentration, measured by HPLC and a method based on the loss of tannins by treatment with granular active carbon (AC), had a very close relationship with the methanogenic toxicity, but a poor relationship was found with the total tannin concentration. The low MW tannins detected by the HPLC and AC methods had similar peak area positions in HPLC chromatograms as the tannins that were adsorbed by polyamide (trisacryl GF05) gel beads. These gel beads have an exclusion limit of 3000 g·mol−1, indicating that this is the approximate MW boundary between toxic and non-toxic tannins. 相似文献
11.
Microbial degradation of tannins – A current perspective 总被引:26,自引:0,他引:26
Tannins are water-soluble polyphenolic compounds having wide prevalence in plants. Hydrolysable and condensed tannins are the two major classes of tannins. These compounds have a range of effects on various organisms – from toxic effects on animals to growth inhibition of microorganisms. Some microbes are, however, resistant to tannins, and have developed various mechanisms and pathways for tannin degradation in their natural milieu. The microbial degradation of condensed tannins is, however, less than hydrolysable tannins in both aerobic and anaerobic environments. A number of microbes have also been isolated from the gastrointestinal tract of animals, which have the ability to break tannin-protein complexes and degrade tannins, especially hydrolysable tannins. Tannase, a key enzyme in the degradation of hydrolysable tannins, is present in a diverse group of microorganisms, including rumen bacteria. This enzyme is being increasingly used in a number of processes. Presently, there is a need for increased understanding of the biodegradation of condensed tannins, particularly in ruminants. 相似文献
12.
Protein-binding capacity of microquantities of tannins 总被引:1,自引:0,他引:1
The physiological effect of tannins is studied in terms of their protein-binding or precipitation capacity. A number of assays based on binding of hemoglobin or bovine serum albumin (BSA) and subsequent determination of unbound protein in supernatant or tannin in a protein-tannin complex are available but with various limitations. These methods are unable to estimate protein-binding capacity, if the quantity of tannin available is low. In the method reported here, tannins or other phenolics were applied on chromatography paper and reacted with BSA and unbound BSA was washed off. The protein in the tannin-protein complex was measured spectrophotometrically after staining with Ponceau S. It required microquantities of sample. Using this method the protein-binding capacity of total leaf extract and hydrolyzable and condensed tannins of Quercus incana, Q. semecarpofolia, and Q. dilatata was determined. The protein binding capacities of ellagic acid and quercetin (microgram BSA/mg) were 297.3 and 78.0, respectively. 相似文献
13.
《Journal of Fermentation Technology》1986,64(6):487-492
A new procedure was developed for the separation of polymeric tannins from a high-tannin white table Koshu wine by extraction with organic solvent and adsorption chromatography. The wine was adjusted to pH 1.0, sodium chloride was added to give a final concentration of 12.5% NaCl, then phenolic compounds including phenolic acids and tannin phenolics were extracted with 2-butanol. About 95% of the total phenolics was extractable.Polymeric tannins were separated from the extract by chromatography on Bio-Gel P-2, which adsorbs polymeric tannins in the presence of 10% acetic acid-50% ethanol, and recovered quantitatively by subsequent elution with 50% acetone. The phenolic acids and flavonoid tannins of low molecular weight that were eluted first using 10% acetic acid-50% ethanol, and the tannin phenolics of high molecular weight subsequently eluted using 50% acetone, were both free of potassium and sodium ions.This method, including the extraction and chromatography, is useful for rough separation of polymeric tannins and other phenolics from white wines. 相似文献
14.
Tree resistance to Lymantria dispar caterpillars: importance and limitations of foliar tannin composition 总被引:1,自引:0,他引:1
Raymond V. Barbehenn Adam Jaros Grace Lee Cara Mozola Quentin Weir Juha-Pekka Salminen 《Oecologia》2009,159(4):777-788
The ability of foliar tannins to increase plant resistance to herbivores is potentially determined by the composition of the
tannins; hydrolyzable tannins are much more active as prooxidants in the guts of caterpillars than are condensed tannins.
By manipulating the tannin compositions of two contrasting tree species, this work examined: (1) whether increased levels
of hydrolyzable tannins increase the resistance of red oak (Quercus rubra L.), a tree with low resistance that produces mainly condensed tannins, and (2) whether increased levels of condensed tannins
decrease the resistance of sugar maple (Acer saccharum Marsh.), a tree with relatively high resistance that produces high levels of hydrolyzable tannins. As expected, when Lymantria dispar L. caterpillars ingested oak leaves coated with hydrolyzable tannins, levels of hydrolyzable tannin oxidation increased in
their midgut contents. However, increased tannin oxidation had no significant impact on oxidative stress in the surrounding
midgut tissues. Although growth efficiencies were decreased by hydrolyzable tannins, growth rates remained unchanged, suggesting
that additional hydrolyzable tannins are not sufficient to increase the resistance of oak. In larvae on condensed tannin-coated
maple, no antioxidant effects were observed in the midgut, and levels of tannin oxidation remained high. Consequently, neither
oxidative stress in midgut tissues nor larval performance were significantly affected by high levels of condensed tannins.
Post hoc comparisons of physiological mechanisms related to tree resistance revealed that maple produced not only higher levels
of oxidative stress in the midgut lumen and midgut tissues of L. dispar, but also decreased protein utilization efficiency compared with oak. Our results suggest that high levels of hydrolyzable
tannins are important for producing oxidative stress, but increased tree resistance to caterpillars may require additional
factors, such as those that produce nutritional stress. 相似文献
15.
A modified method for determining protein binding capacity of plant polyphenolics using radiolabelled protein 总被引:1,自引:0,他引:1
Henson GL Niemeyer L Ansong G Forkner R Makkar HP Hagerman AE 《Phytochemical analysis : PCA》2004,15(3):159-163
A modified radiochemical protein binding method for determining the protein binding capacity of plant polyphenolics (tannins) is described. Purified tannin or unfractionated plant extracts were immobilised on filter paper discs and incubated with the 125I-labelled bovine serum albumin. Protein bound to the disc was proportional to the amount of tannin applied to the disc, although at high concentrations of polyphenolics the discs became saturated and the relationship was no longer applicable. The method was validated using purified procyanidin from Sorghum grain and has been applied to crude polyphenolic extracts from maple, white oak, black oak, walnut and tulip poplar leaves. Specific chemical assays for the determination of proanthocyanidins (acid butanol method) and hydrolysable tannins (modified potassium iodate method) were employed to validate the new protein binding method with the complex plant extracts. 相似文献
16.
The same nine plots were used in this study as in our previous study on inhibition of nitrification (Rice and Pancholy, 1972). These consisted of three stands representing two stages of old field succession and the climax in each of three vegetation types in Oklahoma: tall grass prairie, post oak-blackjack oak forest, and oak-pine forest. Soil samples were analyzed three times during the growing season of 1972 for exchangeable ammonium nitrogen, nitrate, and numbers of Nitrosomonas and Nitrobacter. Results were similar to those obtained during the entire year of 1971. The amount of ammonium nitrogen was lowest in the first successional stage, intermediate in the intermediate successional stage, and highest in the climax. The amount of nitrate was highest in the first successional stage, intermediate in the intermediate successional stage, and lowest in the climax. The numbers of nitrifiers were highest in the first successional stage usually and decreased to a very low number in the climax. These data furnish additional evidence that the nitrifiers are inhibited in the climax so that ammonium nitrogen is not oxidized to nitrate as readily in the climax as in the successional stages. This would aid in the conservation of nitrogen and energy in the climax ecosystem. Some inhibition of nitrification occurred in the intermediate stage of succession also. Previous studies of tannins indicated that these are inhibitory to nitrification, so all important plant species in the intermediate successional stage and the climax were analyzed for total tannin content. A method for extracting and quantifying condensed tannins from soils was developed and the amounts of tannins were determined in each 15-cm level down to 60 cm in the same two plots in each vegetation type. Gallic and ellagic acids, which result from the digestion of hydrolyzable tannins in oak species, were also extracted and quantified in the climax oak-pine forest. All the important herbaceous species, including the grasses, were found to have considerable amounts of condensed tannins. The highest amounts of tannins occurred in the oaks and pine, however. Condensed tannins, hydrolyzable tannins, ellagic acid, gallic acid, digallic acid, and commercial tannic acid (hydrolyzable tannin), in very small concentrations, were all found to completely inhibit nitrification by Nitrosomonas in soil suspensions for 3 weeks, the duration of the tests. Slightly larger concentrations were required to inhibit nitrification by Nitrobacter under similar conditions. The concentrations of tannins, gallic acid, and ellagic acid found in the soil of the research plots were several times higher than the minimum concentrations necessary to completely inhibit nitrification. The inhibition of nitrification was always greater in the climax stand than in the intermediate successional stage in each vegetation type, and the concentration of tannins in the top 15 cm of soil was always higher in the climax stand than in the intermediate successional stage. Moreover, the amounts of tannins calculated to be added to each plot each year are much less than the amounts found in the soil, indicating that the tannins accumulate over a period of time. Thus, it appears that the tannins and tannin derivatives may play a continuous and rather prominent role in the inhibition of nitrification by vegetation. 相似文献
17.
中草药提取物中缩合类单宁的选择性脱除 总被引:1,自引:0,他引:1
以皮胶原纤维为原料,通过甲醛交联反应制备吸附材料。采用外加单宁的方法研究了这种吸附材料对中草药提取物中缩合类单宁的选择性吸附能力。结果表明,胶原纤维吸附材料对落叶松单宁、黑荆树单宁和杨梅单宁等3种典型的缩合类单宁都具有非常好的吸附选择性,在实验条件下单宁的去除率达到100%,而对有效成份的吸附率较低。该吸附材料对与缩合类单宁具有相似结构的低聚原花青素的吸附率也较低。对比试验表明,胶原纤维吸附材料对单宁的吸附选择性优于聚酰胺。 相似文献
18.
Tharayil N Suseela V Triebwasser DJ Preston CM Gerard PD Dukes JS 《The New phytologist》2011,191(1):132-145
? Climate change could increase the frequency with which plants experience abiotic stresses, leading to changes in their metabolic pathways. These stresses may induce the production of compounds that are structurally and biologically different from constitutive compounds. ? We studied how warming and altered precipitation affected the composition, structure, and biological reactivity of leaf litter tannins in Acer rubrum at the Boston-Area Climate Experiment, in Massachusetts, USA. ? Warmer and drier climatic conditions led to higher concentrations of protective compounds, including flavonoids and cutin. The abundance and structure of leaf tannins also responded consistently to climatic treatments. Drought and warming in combination doubled the concentration of total tannins, which reached 30% of leaf-litter DW. This treatment also produced condensed tannins with lower polymerization and a greater proportion of procyanidin units, which in turn reduced sequestration of tannins by litter fiber. Furthermore, because of the structural flexibility of these tannins, litter from this treatment exhibited five times more enzyme (β-glucosidase) complexation capacity on a per-weight basis. Warmer and wetter conditions decreased the amount of foliar condensed tannins. ? Our finding that warming and drought result in the production of highly reactive tannins is novel, and highly relevant to climate change research as these tannins, by immobilizing microbial enzymes, could slow litter decomposition and thus carbon and nutrient cycling in a warmer, drier world. 相似文献
19.
Effects of tannins on Chinese hamster cell line B14 总被引:5,自引:0,他引:5
Tannins, naturally occurring plant phenols, have been recognized as antioxidants, but toxic effects have also been observed. In the current investigation, the interaction of this type of compounds with Chinese hamster cells (cell line B14) has been examined. This study reports on the results of experiments in which B14 cells were exposed to tannins: tannic, ellagic and gallic acids in the concentration range 15-240 microM. The cytotoxic and genotoxic effects of these compounds were studied. The colorimetric MTT assay to assess cytotoxicity and the Comet assay for detection of DNA damage were used. In this paper, we also demonstrated the influence of tannins on the fluidity of the plasma membrane. This experiment was carried out by a spectrofluorometric method using two fluorescent probes: 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) and 12-(9-anthroyloxy)stearic acid (12-AS). The tannins increased the fluidity in the internal region of the lipid bilayer, but no changes at the surface of the plasma membrane were observed. The results of the MTT assay showed that tannins could decrease the viability of cells and that their cytotoxicity was highest at the concentration of 60 microM. The degree of toxicity of these compounds was not correlated with the concentration used. The data obtained from the Comet assay showed that the tannins could also contribute to formation of DNA single-strand breaks. 相似文献
20.
Marja-Riitta Juntheikki 《Biochemical Systematics and Ecology》1996,24(7-8):595-601
Differences in the ability of salivary proteins from Scandinavian and North American moose (Alces alces) to bind tannins from various preferred food plant sources were studied. Both Scandinavian and North American moose produce a salivary tannin-binding protein which binds only condensed tannins common in their diet. The tannins of winter-browsed stems of Pinus sylvestris and Salix pentandra were more effectively bound by salivary tannin-binding protein from North American moose than by those from Scandinavian moose. Tannins of P. sylvestris and S. pentandra stems were also more effective binding agents than tannins from S. caprea and Betula species. The interactions between salivary tannin-binding protein and plant tannins may be one factor affecting food plant choice of moose. The chemical and biochemical characteristics of plant tannins and the ability of salivary tannin-binding proteins to react with tannins should be taken into account in diet selection. 相似文献