岩木瓜茎干化学成分及PTP1B抑制活性研究

综合运用天然产物化学分离、纯化技术从岩木瓜茎干中分离得到18个化合物,结合各化合物理化性质和光谱数据鉴定其结构,依次为对羟基苯乙酮(1)、对羟基苯甲酸(2)、对羟基苯甲酸乙酯(3)、3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-1-one(4)、咖啡酸(5)、咖啡酸乙酯(6)、对羟基苯甲醛(7)、咖啡酸甲酯(8)、对羟基桂皮酸(9)、水杨酸(10)、原儿茶酸(11)、3,5-二羟基-4-甲氧基苯甲酸(12)、对醛基苯基-1-O-葡萄糖苷(13)、色氨酸(14)、豆甾-3,6-二酮(15)、β-谷甾酮(16)、5α-9(11)-豆甾烯-3β-醇(17)和胡萝卜苷(18)。这些化合物均为首次从该植物分离得到,其中化合物4,6,16和17为首次从该属植物中分离得到。对从岩木瓜中分离得到的46个化合物进行PTP1B抑制活性筛选,11个化合物具有PTP1B抑制活性,IC50值在1.0~37.0μM之间。     

黄桐树皮的化学成分研究

从黄桐(Endospermum chinense Benth.)树皮中分离获得了10个化合物。通过光谱分析,分别鉴定为3-羰基齐墩果酸(1)、齐墩果酸(2)、7-羰基谷甾醇(3)、β-谷甾醇(4)、β-胡萝卜甙(5)、7-羰基-β-胡萝卜甙(6)、4-羟基-3-甲氧基苯甲醛(7)、7-羟基-6-甲氧基香豆素(8)、3-羟基-4-甲氧基苯甲酸(9)和壬二酸(10)。10个化合物均为首次从黄桐树皮中分离得到。     

马槟榔果实的化学成分研究（英文）

采用硅胶柱色谱和Sephadex LH-20等分离方法,对马槟榔Capparis masaikai果实的化学成分进行分离纯化,依据理化性质及波谱数据分析进行结构鉴定,从中分离鉴定了10个单体化合物,分别为:杜仲树脂酚(1)、erythro-guaiacylglycerol-β-O-4'-sinapyl ether(2)、hedyotol C(3)、hedyotisol A(4)、hedyotisol B(5)、ozoroalide(6)、5α,6α-epoxy-3β-hydroxyergosta-22-ene-7-one(7)、松柏醛(8)、3-羟基-5-(对羟基苯基)戊酸(9)、β-hydroxypropiovanillone(10)。分离得到的化合物结构类型包括木脂素、大环内酯、甾醇及酚类。化合物1~10为首次从该植物中分离,其中1~7为首次从该属植物中分离得到。     

红波罗花的化学成分研究(英文)

从红波罗花(Incarvillea delavayi)植物的干燥全草乙醇提取物中分离得到12个化合物,通过MS和NMR等方法鉴定为tecomine(1)、epidihydrotecomanine(2)、2,6-二甲基-6-羟基-2-庚烯-4-酮(3)、marine B(4)、(3R,5S)-3-methyl-cyclopentyl[1,2-c]pyridine-5-ol(5)、3,4,5-三甲氧基苯甲酸乙酯(6)、cleroindicin B(7)、3,4,5-三甲氧基苯甲酸甲酯(8)、2-(4’-乙氧基苯基)-乙醇(9)、6-羟基苯并二氢呋喃(10)、β-谷甾酮(11)、β-乙酰齐墩果酸(12)。其中化合物1～3、5～6、8～12为首次从角蒿属植物中分离得到,所有化合物均为首次从该植物中分离得到。     

米邦塔仙人掌化学成分研究

从米邦塔仙人掌正丁醇部分分离到10个化合物。应用色谱和光谱分析方法分别鉴定为对羟基苯甲酸(1)、β-羟基-3-甲氧基-4-羟基苯乙酮(2)、2,3,4-三羟基戊酸(3)、4-甲氧基桂皮酸(4)、3-羟基-4-甲氧基肉桂酸(5)、5-羟基阿魏酸(6)、4-羟基肉桂酸(7)、原儿茶酸(8)、n-butyl eucomate(9)和eucomic acid(10)。化合物2～10为首次从该植物分离得到。     

藏药翁布的黄酮类化学成分研究

从藏药翁布(Myricaria germanica)的60%丙酮提取物中分离得到了11个黄酮类化合物,利用光谱、波谱分析法鉴定其结构分别为:35,,4’-三羟基-73,’-二甲氧基黄酮(1),3,5,4’-三羟基-7-甲氧基黄酮(2),3,5,3’,4’-四羟基-7-甲氧基黄酮(3),35,7,-三羟基-4’-甲氧基黄酮(4),柽柳素(5),山柰酚(6),槲皮素-3-O-β-D-葡萄糖苷(7)5,,7,4’-三羟基-3-O-β-D-葡萄糖醛酸(8),5,7,3’4,’-四羟基-3-O-β-D-葡萄糖醛酸(9),槲皮苷(10)和阿福豆苷(11)。上述化合物均为首次从该植物中分离得到,其中化合物4、5、7～11为首次从水柏枝属植物中分离得到。     

珍珠菜的化学成分研究(英文)

从珍珠菜(Lysimachia clethroidesDuby)的70%丙酮提取物中分离得到11个化合物,经理化性质及波谱数据分析分别鉴定为23-羟基乌苏酸(1)、Isotachioside(2)、对羟基苯甲酸(3)、3-甲氧基-4-羟基苯甲酸(4)、原儿茶酸(5)、3,5-二羟基苯甲酸(6)、甲基-α-D-呋喃果糖苷(7)、尿苷(8)、β-谷甾醇(9)、胡萝卜苷(10)和齐墩果酸(11)。化合物1、2、4、7和8为首次从该属植物中分离得到,化合物3、5、6、9~11为首次从该种植物中分离得到。     

小叶米仔兰枝条中的抗菌活性成分研究

为探讨小叶米仔兰(Aglaia odorata var.microphyllina)中的抗菌活性成分,从其枝条的乙醇提取物中分离得到6个化合物,通过波谱分析,分别鉴定为:4(15)-桉叶烯-1β,7,11-三醇(1)、4α,10β,11-三羟基-1,5-反-愈创木烷-6-烯(2)、6,15α-环氧-1β,4β-二羟基桉烷(3)、2-羟基-丁二酸-1-甲酯(4)、巴西红厚壳素(5)和胡萝卜苷(6)。以上化合物均为首次从该植物中分离得到,其中化合物1、2、4和5为首次从米仔兰属植物中分离得到。用滤纸片琼脂扩散法测定了化合物1~5的抗菌活性,结果显示化合物4和5对金黄色葡萄球菌有抑制作用。     

芒果叶化学成分研究Ⅲ

为了解芒果(Mangifera indica L.)的化学成分,从芒果叶70%乙醇提取物中分离鉴定了8个单体化合物,经波谱分析,分别鉴定为:5-(β-D-glucopyranosyloxy)-2-hydroxy benzoic acid methyl ester(1)、methyl salicylate glucoside(2)、对羟基苯甲酸(3)、nikoenoside(4)、(6R,9R)-3-oxo-α-ionol-β-D-glucopyranoside(5)、byzantionoside B(6)、icariside B2(7)和2-ethyl-3-methylmaleimide N-β-D-glucopyranoside(8)。化合物1、2、4~7均为首次从芒果属中分离得到,化合物8首次从该种中分离得到,化合物1的NMR数据是首次报道。     

蝴蝶花的化学成分研究

对鸢尾科鸢尾属植物蝴蝶花全草进行化学成分和生物活性研究。通过正相硅胶、Sephadex LH-20以及反相HPLC柱等等色谱方法对蝴蝶花的化学成分进行分离纯化,应用谱学技术鉴定化合物的结构。从蝴蝶花95%乙醇提取物中分离得到18个化合物,分别鉴定为:(+)-(7S,8R,7'E)-4-hydroxy-3,5'-dimethoxy-4',7-epoxy-8,3'-neolign-7'-ene-9,9'-diol 9'-ethyl ether(1)、丁香脂素(2)、erythro-guaiacylglycerol-β-O-4'-coniferylether(3)、threo-guaiacylglycerol-β-O-4'-coniferylether(4)、4-O-β-d-(6-O-vanilloylglucopyranosyl)vanillic acid(5)、4-[6-O-(4-hydroxy-3,5-dimethoxybenzoyl)-β-d-glucopyranosyloxy]-3-methoxybenzoic acid(6)、藏黄连新苷B(7)、belalloside B(8)、藏黄连新苷A(9)、茶叶花宁(10)、sakuranetin(11)、鼠李柠檬素(12)、鸢尾甲黄素B(13)、染料木素(14)、5,7,4'-三羟基-6,8-二甲氧基异黄酮(15)、野鸢尾苷元(16)、8-羟基鸢尾苷元(17)、3'-O-methylorobol(18)。其中,化合物1~18均为首次从该种植物中分离得到。化合物1、2、5、6、15为首次从该属植物中分离得到。对所分离得到的化合物进行了保肝、抗炎、抗氧化等体外药理活性筛选。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.