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1.
Fed-batch is the dominating mode of operation in high-cell-density cultures of Saccharomyces cerevisae in processes such as the production of baker's yeast and recombinant proteins, where the high oxygen demand of these cultures makes its supply an important and difficult task. The aim of this work was to study the use of hyperbaric air for oxygen mass transfer improvement on S. cerevisiae fed-batch cultivation. The effects of increased air pressure up to 1.5 MPa on cell behavior were investigated. The effects of oxygen and carbon dioxide were dissociated from the effects of total pressure by the use of pure oxygen and gas mixtures enriched with CO(2). Fed-batch experiments were performed in a stirred tank reactor with a 600 mL stainless steel vessel. An exponential feeding profile at dilution rates up to 0.1 h(-)(1) was used in order to ensure a subcritical flux of substrate and, consequently, to prevent ethanol formation due to glucose excess. The ethanol production observed at atmospheric pressure was reduced by the bioreactor pressurization up to 1.0 MPa. The maximum biomass yield, 0.5 g g(-)(1) (cell mass produced per mass of glucose consumed) was attained whenever pressure was increased gradually through time. This demonstrates the adaptive behavior of the cells to the hyperbaric conditions. This work proved that hyperbaric air up to 1.0 MPa (0.2 MPa of oxygen partial pressure) could be applied to S. cerevisiae cultivation under low glucose flux. Above that critical oxygen partial pressure value, i.e., for oxygen pressures of 0.32 and 0.5 MPa, a drastic cell growth inhibition and viability loss were observed. The increase of carbon dioxide partial pressure in the gas mixture up to 48 kPa slightly decreased the overall cell mass yield but had negligible effects on cell viability. 相似文献
2.
Oxidative stress response of <Emphasis Type="Italic">Kluyveromyces marxianus</Emphasis> to hydrogen peroxide,paraquat and pressure 总被引:1,自引:0,他引:1
The aim of this work was to study the oxidative stress response of Kluyveromyces marxianus to hydrogen peroxide (50 mM), paraquat (1 mM), an increase in air pressure (120 kPa, 600 kPa) and pure oxygen pressure (120-600 kPa) in a pressurized bioreactor. The effect of these oxidants on metabolism and on the induction of antioxidant enzymes was investigated. The exposure for 1 h of K. marxianus at exponential growth phase with either H(2)O(2) or paraquat, under air pressure of 120 kPa or 600 kPa, induced an increase in both superoxide dismutase (SOD) and glutathione reductase (GR) content. SOD induction by the chemical oxidants was independent of the air pressure values used. A 2-fold increase in SOD activity was observed after 1 h of exposure to H(2)O(2) and a 3-fold increase was obtained by the presence of paraquat, with both air pressures studied. In contrast, GR activity was raised 1.7-fold by the exposure to both chemicals with 120 kPa, but a 2.4-fold GR induction was obtained with 600 kPa. As opposed to Saccharomyces cerevisiae, catalase was not induced and was even lower than the normal basal levels. This antioxidant enzyme seemed to be inhibited under increasing oxygen partial pressure. The cells showed a significant increase in SOD and GR activity levels, 4.7-fold and 4.4-fold, when exposed for 24 h to 120 kPa pure oxygen pressure. This behaviour was even more patent with 400 kPa. However, whenever cells were previously exposed to low air pressures, low enzymatic activity levels were measured after subsequent exposure to pure oxygen pressure. 相似文献
3.
The use of air pressure as a way of improving oxygen transfer in aerobic bioreactors was investigated. To compare the air pressure effects with traditional air bubbled cultures, experiments using a pressure reactor and a stirred flask, with the same oxygen transfer rate, were made. Kluyveromyces marxianus is an important industrial yeast and some of it show a “Kluyver effect” for lactose: even under oxygen limited growth conditions, certain disaccharides that support aerobic, respiratory growth, are not fermented. This study deals with the effect of increased pressure on the physiological behavior of two Kluyveromyces strains: K. marxianus ATCC10022 is a lactose-fermenting strain, whereas K. marxianus CBS 7894 has a Kluyver-effect for lactose. For K. marxianus ATCC10022 an air pressure increase of 2 bar led to a 3-fold increase in biomass yield. When air pressure increased an enhancement of ethanol oxidation of cell yeasts was also observed. Batch cultures of K. marxianus CBS 7894 exhibited different growth behaviour. Its metabolism was always oxidative and ethanol was never produced. With the increase in air pressure, it was possible to increase the productivity in biomass of K. marxianus CBS 7894. As a response to high oxygen concentrations, due to the increase in oxygen partial pressure, oxidative stress in the cells was also studied. Antioxidant defences, such as superoxide dismutase, catalase, and glutathione reductase, were at high activity levels, suggesting that these yeast strains could tolerate the increased pressures applied. 相似文献
4.
Onken U 《Biotechnology and bioengineering》1990,35(10):983-989
The effect of increased total pressure and partial pressures of oxygen and carbon dioxide on the growth of Pseudomonas fluorescens was investigated in an airlift reactor. In batch cultivations bacterial growth was completely inhibited with air at 8 bars total pressure. The same effect was observed with aeration by pure oxygen at 1.15 bars. Carbon dioxide partial pressure did not show inhibitory effects. Continuous experiments confirm the assumption that growth inhibition at higher total pressure is caused by the increase in oxygen partial pressure. Incubation of P. fluorescens at higher oxygen partial pressure led to an increase of bacterial productivity during subsequent continuous cultivation at ambient pressure (1 bar) with air. Maximum productivity was increased by about 75% after aeration with pure oxygen. This effect is probably the result of metabolic adaption of the bacterial cells to high oxygen partial pressure. 相似文献
5.
Coelho MA Belo I Pinheiro R Amaral AL Mota M Coutinho JA Ferreira EC 《Applied microbiology and biotechnology》2004,66(3):318-324
The effects of hyperbaric stress on the morphology of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 MPa and 0.6 MPa and different gas compositions (air, oxygen, nitrogen or carbon dioxide), covering aerobic and anaerobic conditions. A method using automatic image analysis for classification of S. cerevisiae cells based on their morphology was developed and applied to experimental data. Information on cell size distribution and bud formation throughout the cell cycle is reported. The results show that the effect of pressure on cell activity strongly depends on the nature of the gas used for pressurization. While nitrogen and air to a maximum of 0.6 MPa of pressure were innocuous to yeast, oxygen and carbon dioxide pressure caused cell inactivation, which was confirmed by the reduction of bud cells with time. Moreover, a decrease in the average cell size was found for cells exposed for 7.5 h to 0.6 MPa CO2. 相似文献
6.
Saccharomyces cerevisiae, in a pressure batch reactor, coped with higher air (1.2-3 bar) pressures better than with pure oxygen pressures (1.2-3 bar) for an equivalent dissolved oxygen concentration. However, pure oxygen pressure enhanced ethanol production. Both pressures did not influence the type of metabolism followed by the organism which was always oxidoreductive. Growth was inhibited with the increase of air and pure oxygen pressure and almost completely inhibited with 8 bar of pure oxygen. Above 3 bar activities of mitochondrial superoxide dismutase and glutathione reductase increased with air pressure, but cytosolic superoxide dismutase and catalase increased activity only in pure oxygen pressure. 相似文献
7.
Gas Exchange of Algae: II. Effects of Oxygen, Helium, and Argon on the Photosynthesis of Chlorella pyrenoidosa 总被引:3,自引:3,他引:0
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Changes in the oxygen partial pressure of air over the range of 8 to 258 mm of Hg did not adversely affect the photosynthetic capacity of Chlorella pyrenoidosa. Gas exchange and growth measurements remained constant for 3-week periods and were similar to air controls (oxygen pressure of 160 mm of Hg). Oxygen partial pressures of 532 and 745 mm of Hg had an adverse effect on algal metabolism. Carbon dioxide consumption was 24% lower in the gas mixture containing oxygen at a pressure 532 mm of Hg than in the air control, and the growth rate was slightly reduced. Oxygen at a partial pressure of 745 mm of Hg decreased the photosynthetic rate 39% and the growth rate 37% over the corresponding rates in air. The lowered metabolic rates remained constant during 14 days of measurements, and the effect was reversible after this time. Substitution of helium or argon for the nitrogen in air had no effect on oxygen production, carbon dioxide consumption, or growth rate for 3-week periods. All measurements were made at a total pressure of 760 mm of Hg, and all gas mixtures were enriched with 2% carbon dioxide. Thus, the physiological functioning and reliability of a photosynthetic gas exchanger should not be adversely affected by: (i) oxygen partial pressures ranging from 8 to 258 mm of Hg; (ii) the use of pure oxygen at reduced total pressure (155 to 258 mm of Hg) unless pressure per se affects photosynthesis, or (iii) the inclusion of helium or argon in the gas environment (up to a partial pressure of 595 mm of Hg). 相似文献
8.
Increased cellular levels of reactive oxygen species are known to arise during exposure of organisms to elevated metal concentrations, but the consequences for cells in the context of metal toxicity are poorly characterized. Using two-dimensional gel electrophoresis, combined with immunodetection of protein carbonyls, we report here that exposure of the yeast Saccharomyces cerevisiae to copper causes a marked increase in cellular protein carbonyl levels, indicative of oxidative protein damage. The response was time dependent, with total-protein oxidation peaking approximately 15 min after the onset of copper treatment. Moreover, this oxidative damage was not evenly distributed among the expressed proteins of the cell. Rather, in a similar manner to peroxide-induced oxidative stress, copper-dependent protein carbonylation appeared to target glycolytic pathway and related enzymes, as well as heat shock proteins. Oxidative targeting of these and other enzymes was isoform-specific and, in most cases, was also associated with a decline in the proteins' relative abundance. Our results are consistent with a model in which copper-induced oxidative stress disables the flow of carbon through the preferred glycolytic pathway, and promotes the production of glucose-equivalents within the pentose phosphate pathway. Such re-routing of the metabolic flux may serve as a rapid-response mechanism to help cells counter the damaging effects of copper-induced oxidative stress. 相似文献
9.
《Process Biochemistry》2007,42(10):1378-1383
The effects of hyperbaric gases on the cell cycle of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 and 0.6 MPa and different gas compositions (air, oxygen, nitrogen or carbon dioxide). Classification of S. cerevisiae cells based on their morphology stages was obtained using an automatic image analysis procedure. Information on the distribution of different sub-populations along the cell cycle is reported. A structured morphological model was developed and used to describe the measured data. The results herein reported demonstrate that the bud separation phase is the limiting step in cell duplication. Additionally, the influence of the environmental conditions, specially the oxygen partial pressure, on the START event is reported. Under anaerobic conditions, no significant influence of hyperbaric gases on the cell cycle was verified. 相似文献
10.
Lushchak O Lozins'kyĭ O Nazarchuk T Lushchak V 《Ukrainski? biokhimicheski? zhurnal》2008,80(2):106-113
Possible role of catalases in modification of yeast Saccharomyces cerevisiae response to nitrosative stress was studied in the work. Yeast cell of a wild strain and catalase-defective strains were treated with nitric oxide donor S-nitrosoglutathione, then the cell survival rate, and the levels of protein carbonyls and oxidized glutathione were measured. It was shown that S-nitrosoglutathione decreased cells viability of wild and catalase-defective strains. Unexpected, yeast cells defective by both catalases survived successfully as compared with the cells of the wild strain. The intensity of stress was evaluated by measures of oxidative protein modifications and glutathione oxidation. Treatment with S-nitrosoglutathione did not affect the level of protein carbonyls and was lower by about 14 i 22 % in the cells of double mutant strains after treatments with S-nitrosoglutathione in concentrations of 10 and 20 mM. S-nitrosoglutathione induced a strong increase of the level of oxidized glutathione in yeast cells of the wild strain. This stress slightly increased the level of oxidized glutathione in the yeast cells defective by peroxisomal or both catalases. It is interesting, that an increase of oxidized glutathione level was not observed in the yeast cells defective by cytosolic catalase. The obtained results prove that catalases can modify yeast cell response to the nitrosative stress. 相似文献
11.
12.
The present study was undertaken to determine the effect of cadmium (Cd) on the antioxidant status of the yeast Saccharomyces cerevisiae. S. cerevisiae serves as a good eukaryotic model system for the study of the molecular mechanisms of oxidative stress. We investigated the adaptative response of S. cerevisiae exposed to Cd. Yeast cells could tolerate up to 100 microM Cd and an inhibition in the growth and viability was observed. Exposure of yeast cells to Cd showed an increase in malondialdehyde and glutathione. The activities of catalase, superoxide dismutase and glutathione peroxidase were also high in Cd-exposed cells. The incorporation of Cd led to significant increase in iron, zinc and inversely the calcium, copper levels were reduced. The results suggest that antioxidants were increased and are involved in the protection against macromolecular damage during oxidative stress; presumably, these enzymes are essential for counteracting the pro-oxidant effects of Cd. 相似文献
13.
L Estrada-García J Carrera-Rotllan P Puig-Parellada 《Prostaglandins & other lipid mediators》2002,67(1):13-25
We analyzed the influence of oxidative stress and agents that modify its effect in human umbilical vein endothelial cell cultures (HUVEC). The parameters analyzed were PGI2, TXA2, PGI2/TXA2 ratio, lipid peroxidation and cell viability. Oxidative stress was induced by H2O2. The agents (treatments) that were tested are: antioxidant enzymes (superoxide dismutase and catalase), oxygen free radical scavenger (vitamin E) and eicosanoids of the series 2 and 3 (Arachidonic acid, Eicosapentanoic acid). In this study we show, in long term endothelial cell cultures, the effects of different levels of oxidative stress alone or in combination with the different treatment agents, over the analyzed parameters. With induced oxidative stress alone the results obtained indicate that it has a harmful effect over cell function and viability, and that this effect is dose and time dependent. In absence of oxidative stress in basal situation, none of the treatments assayed showed significant differences compared to control cultures in the different analyzed parameters. When oxidative stress increased, antioxidant enzymes reduced cell damage and had a protective function, whereas Eicosapentanoic acid and vitamin E presented a lower level of protection. No beneficial effect was observed with arachidonic acid treatments. A significant increase in cell survival was observed in culture cells with oxidative stress when they were treated with antioxidant enzymes. 相似文献
14.
以高纯空气(O2∶N2 =21∶79)为加压介质,研究了0.5Mpa压力下面包酵母CICC1447和CICC1339细胞生长及细胞内谷胱甘肽、麦角固醇的含量变化。结果表明:加压培养时两株酵母菌的对数生长期延迟出现,对数生长期的持续时间缩短,而且两株菌的比生长速率均明显低于对照组,同时两株菌的倍增时间也较对照组有所延长;压力刺激可显著提高面包酵母细胞内谷胱甘肽(GSH)的含量,但麦角固醇含量的变化却不明显。在0.5MPa压力下保压培养3h时CICC1447胞内谷胱甘肽含量比常压对照组提高了42.6%,而加压3h后麦角固醇含量比对照组提高了20.1%;加压培养6h时CICC1339胞内谷胱甘肽含量较对照组提高了58.7%,但其麦角固醇含量反而降低。这说明不同的酵母菌对压力刺激的反应是不同的。 相似文献
15.
Changes of CYP1A1, GST, and ALDH3 enzymes in hepatoma cell lines undergoing enhanced lipid peroxidation 总被引:1,自引:0,他引:1
Bassi AM Ledda S Penco S Menini S Muzio G Canuto R Ferro M 《Free radical biology & medicine》2000,29(11):1186-1196
Hepatoma cells show alterations in the response to oxidative stress (decreased lipid peroxidation) and in xenobiotic metabolism enzymes (decreased P450, increased GST and ALDH3). This study examined the effect of lipid peroxidation on the expression of the above enzymes in two rat hepatoma cell lines (MH(1)C(1) and 7777). To induce oxidative stress, cells were exposed to arachidonic acid (to increase lipid peroxidation substrate) and/or to beta-naphthoflavone (to increase CYP450), and treated with one dose of iron/histidine. The cells, that were still viable after the challenge, were refed with the culture medium and CYP1A1, GST, and ALDH3 enzymes monitored for 1, 6, 12, and 24 h. Treatments that increased markers indicative of lipid peroxidation are associated with a decrease in enzyme activities, which was permanent for CYP1A1 and transient for the other enzymes. We speculate from these data that aldehydic byproducts of lipid peroxidation may be responsible for these effects. Thus, restoration of lipid peroxidation in hepatoma cells seems to induce a rapid adaptation to oxidative stress, which is achieved by a simultaneous decrease of reactive oxygen species production and an increase in the two main enzymes involved in the removal of the aldehydic products of lipid peroxidation. 相似文献
16.
5-Hydroxymethyluracil-DNA glycosylase activity may be a differentiated mammalian function 总被引:6,自引:0,他引:6
To determine the prevalence of the repair enzyme HMU-DNA glycosylase we assayed its activity in whole cell extracts of several bacterial species, the eukaryotic yeast Saccharomyces cerevisiae, mammalian cell lines and murine tissue. Enzyme activity was constitutively present in murine, hamster and human cell lines. It was not inducible by exposing cells to oxidative stress from ionizing radiation or by incubating cells with the 2'-deoxynucleoside of HMU, HMdU. In murine tissue, enzyme activity was highest in brain and thymus. HMU-DNA glycosylase activity was not detectable in bacteria or yeast nor could activity be detected after exposure of cells to H2O2. These results suggest that, in contrast to other DNA-repair enzymes, HMU-DNA glycosylase is a differentiated function limited to higher eukaryotic organisms. 相似文献
17.
The effects of increased air pressure in a culture of the thermophilic microorganism Thermus sp. RQ-1 were investigated. Cell growth dependence on oxygen supply was investigated in a fermenter at atmospheric pressure.
Total oxygen depletion from the medium for low values of k
La was observed during the exponential growth phase. It was possible with this strain to enhance the oxygen transfer rate by
increasing the air pressure. Cell productivity was improved by pressurisation up to 0.56 MPa for batch cultivation; and an
induction of the antioxidant enzymes, superoxide dismutase and catalase, was observed with the rise in pressure. Cell pre-cultivation
under pressurised conditions conferred to the cells more resistance to an exposure to hydrogen peroxide and more sensitivity
to paraquat (methyl viologen). The usefulness of bioreactor pressurisation on the cultivation of Thermus sp. RQ-1 was demonstrated for fed-batch operation, with the attainment of higher cell densities. A two-fold increase in cell
mass productivity was obtained by the use of hyperbaric air (0.5 MPa). With the pressurisation of the head-space in the reactor,
it was also possible to eliminate the loss of liquid by evaporation, which amounted to more than 10% at 70 °C and atmospheric
pressure.
Received: 16 July 1999 / Revision received: 26 November 1999 / Accepted: 28 November 1999 相似文献
18.
Batch cultures of E. coli TB1/pUC13 were carried out at different oxygen transfer rates (OTR) enhanced by the increase of stirring rate and by the increase of air total pressure of the bioreactor. These two variables showed to have little effect on cell growth but a negative effect on cytochrome b5 (recombinant protein) production. However, this effect was more significant of high stirring rates than for values of pressure up to 0.4?MPa. The effects of stirring and pressure were also investigated for fed-batch mode operation. In this type of cell cultivation high cell densities are reached, thus a high capacity of oxygen supply of the system is required. To compare the two ways of improving OTR, cell behaviour was followed in two bioreactors at different operational conditions giving the same maximum OTR value. The first one operated at a high stirring rate (500?rpm) and at atmospheric pressure (0.1?MPa) and the other one at high air pressure (0.48?MPa) and low stirring rate. The increased pressure seemed to be a better way of ensuring an adequate oxygen supply to a culture of E. coli TB1 cells than an increased stirring rate. For the high pressure experiment a higher cellular density was reached, as well as a higher cyt.b5 expression which led to a 4-fold increase in final productivity. These experiments showed that bioreactor pressurization can be successfully used as a means of enhancing oxygen mass transfer to shear sensitive cell cultures. 相似文献
19.
Aertsen A De Spiegeleer P Vanoirbeek K Lavilla M Michiels CW 《Applied and environmental microbiology》2005,71(5):2226-2231
Using leaderless alkaline phosphatase as a probe, it was demonstrated that pressure treatment induces endogenous intracellular oxidative stress in Escherichia coli MG1655. In stationary-phase cells, this oxidative stress increased with the applied pressure at least up to 400 MPa, which is well beyond the pressure at which the cells started to become inactivated (200 MPa). In exponential-phase cells, in contrast, oxidative stress increased with pressure treatment up to 150 MPa and then decreased again, together with the cell counts. Anaerobic incubation after pressure treatment significantly supported the recovery of MG1655, while mutants with increased intrinsic sensitivity toward oxidative stress (katE, katF, oxyR, sodAB, and soxS) were found to be more pressure sensitive than wild-type MG1655. Furthermore, mild pressure treatment strongly sensitized E. coli toward t-butylhydroperoxide and the superoxide generator plumbagin. Finally, previously described pressure-resistant mutants of E. coli MG1655 displayed enhanced resistance toward plumbagin. In one of these mutants, the induction of endogenous oxidative stress upon high hydrostatic pressure treatment was also investigated and found to be much lower than in MG1655. These results suggest that, at least under some conditions, the inactivation of E. coli by high hydrostatic pressure treatment is the consequence of a suicide mechanism involving the induction of an endogenous oxidative burst. 相似文献
20.
Protective effect of antioxidants against para-nonylphenol-induced inhibition of cell growth in Saccharomyces cerevisiae 总被引:4,自引:0,他引:4
Okai Y Higashi-Okai K Machida K Nakamura H Nakayama K Fujita K Tanaka T Otani S Taniguchi M 《FEMS microbiology letters》2000,185(1):65-70
The cell growth-modulating activity of an endocrine disruptor, p-nonylphenol (NP), was estimated using the yeast Saccharomyces cerevisiae as a simple model of eukaryotic cells. NP caused a dose-dependent suppressive effect on cell growth of S. cerevisiae at 10, 25 and 50 microM. The NP-induced cell growth inhibition was restored when concomitantly lipophilic antioxidants such as alpha-tocopherol and beta-carotene were supplied, but not the hydrophilic antioxidants ascorbic acid or (-)epigallocatechin gallate (EGCG). The cellular oxygen consumption of S. cerevisiae was also inhibited in a dose-dependent fashion by the extracellular addition of NP, and pretreatment with alpha-tocopherol and beta-carotene suppressed NP-induced inhibition of cellular oxygen consumption, but ascorbic acid and EGCG were not effective. Furthermore, NP caused a marked generation of radical oxygen species (ROS) in S. cerevisiae, which was suppressed by treatment with alpha-tocopherol and beta-carotene, but not with ascorbic acid and EGCG. However, NP did not show a significant inhibitory effect on cell growth and survival of mitochondria-deficient petite mutant cells and they showed a relatively weak ROS-generating activity compared with parent yeast cells. These results suggest that NP-induced inhibition of cell growth and oxygen consumption in S. cerevisiae might be possibly associated with ROS generation in yeast mitochondria. The significance of this finding is discussed from the viewpoint of NP-induced oxidative stress against eukaryotic cells. 相似文献