Phytoplankton production and growth rate in Lake Tanganyika: evidence of a decline in primary productivity in recent decades

1. This study focused on phytoplankton production in Lake Tanganyika. We provide new estimates of daily and annual primary production, as well as growth rates of phytoplankton, and we compare them with values published in former studies.
2. Chlorophyll- a (chl- a ) in the mixed layer ranged from 5 to 120 mg chl- a  m⁻² and varied significantly between rainy and dry seasons. Particulate organic carbon concentrations were significantly higher in the south basin (with 196 and 166 mg C m⁻³ in the dry and the rainy season, respectively) than in the north basin (112 and 109 mg C m⁻³, respectively).
3. Carbon : phosphorus (C : P) ratios varied according to season. Phosphorus limitation seemed to occur more frequently than nitrogen limitation, especially during the rainy season. Severe P deficiencies were rare.
4. Measured particulate daily primary production ranged from 110 to 1410 mg C m⁻² day⁻¹; seasonal contrasts were well marked in the north basin, but less in the south basin, where primary production peaks occurred also in the rainy season. Estimates of annual primary production, based on daily primary production calculated from chl- a and water transparency, gave values lower than those reported in previous studies. Picophytoplankton accounted on average for 56% of total particulate production in the south basin during the wet season of 2003.
5. Phytoplankton growth rates, calculated from primary production, ranged from 0.055 to 0.282 day⁻¹; these are lower than previously published values for Lake Tanganyika.     

Population dynamics and production of Jesogammarus annandalei, an endemic amphipod, in Lake Biwa, Japan

SUMMARY 1. Population dynamics and production of Jesogammarus annandalei , an endemic amphipod in Lake Biwa, were examined from April 1997 to June 1998. The life cycle of this species was 1 year with the new generation beginning in early autumn. They preferred low temperature (<12 °C) and their spatial distribution varied seasonally and accordingly.
2. In deep water, the abundance of J. annandalei ranged from 200 to 63 000 m⁻² and decreased towards summer and the biomass (0.01∼3.6 g C m⁻²) was on average comparable that of zooplankton. The density was much higher than that recorded by a study conducted 35 years ago.
3. Individual growth rate of this amphipod was high in winter and spring but decreased in summer. Annual production of J. annandalei (6.2 g C m⁻² year⁻¹) was only 2% of primary production but was at the higher end of the range reported for amphipods in oligo- and mesotrophic lakes.
4. These results are consistent with the view that Lake Biwa is becoming more eutrophic, with a consequent decrease in the abundance of predatory fish in the profundal zone.     

Long-term effects of successive Ca(OH)2 and CaCO3 treatments on the water quality of two eutrophic hardwater lakes

1. Whole-lake experiments were conducted in two hardwater lakes (Halfmoon and Figure Eight) in Alberta, Canada, to investigate the effectiveness of repeated lime (slaked lime: Ca(OH)₂ and/or calcite: CaCO₃) treatments (5–78 mg L^–1) for up to 7 years.
2. Randomized intervention analysis of intersystem differences between the experimental and three reference lakes demonstrated a decline in euphotic total phosphorus and chlorophyll a concentrations in the experimental lakes after repeated lime treatments.
3. After the second lime application to Halfmoon Lake, mean winter total phosphorus release rates (TPRR) decreased to < 1 mg m^–2 day^–1 compared with 3.6 mg m^–2 day^–1 during the winter after initial treatment. In the final year of lime application, mean summer TPRR decreased to 4.5 mg m^–2 day^–1 compared with 7.6 mg m^–2 day^–1 in the pre-treatment year.
4. Mean macrophyte biomass declined and species composition was altered at 1 and 2 m depths in Figure Eight Lake during lime application. Over the first 6 years of treatment, macrophyte biomass at 2 m declined by 95% compared with concentrations recorded during the initial treatment year. In the last year of the study, macrophyte biomass at 2 m reached initial treatment concentrations, which coincided with the greatest water transparency. Over the treatment period, macrophyte species shifted from floating to rooted plants.
5. Multiple lime applications can improve water quality in eutrophic hardwater lakes for periods of up to 7 years.     

Formation and microbial community analysis of chloroanilines-degrading aerobic granules in the sequencing airlift bioreactor

Aims:  This paper investigates a selection-based acclimation strategy for improving the performance and stability of aerobic granules at a high chloroanilines loading.
Methods and Results:  The experiments were conducted in a sequencing airlift bioreactor (SABR) to develop aerobic granules fed with chloroanilines (ClA). The evolution of aerobic granulation was monitored using image analysis and scanning electron microscopy, and PCR–DGGE analysis of microbial community was performed. The sludge granulation was apparently developed by decreased settling time and gradual increased ClA loading to 0·8 kg m⁻³ day⁻¹. A steady-state performance of the granular SABR was reached at last, as evidenced by biomass concentration of 6·3 g l⁻¹ and constant ClA removal efficiency of 99·9%. The mature granules had a mean size of 1·55 mm, minimal settling velocity of 68·4 m h⁻¹, specific ClA degradation rate of 0·181 g gVSS⁻¹ day⁻¹. Phylogenetic analysis of aerobic ClA-degrading granules confirmed the dominance of β - , γ -Proteobacteria and Flavobacteria.
Conclusions:  The chosen operating strategy involving step increase in ClA loading and enhancement of major selection pressures was successful in cultivating the aerobic ClA-degrading granules.
Significance and Impact of the Study:  This research could be helpful for improving the stability of aerobic granules via optimizing operating conditions and developing economic feasible full-scale granular bioreactor.     

A simple model of the eco-hydrodynamics of the epilimnion of Lake Tanganyika

1. The ecosystem response of Lake Tanganyika was studied using a four-component, nutrient–phytoplankton–zooplankton–detritus, phosphorus-based ecosystem model coupled to a nonlinear, reduced-gravity, circulation model. The ecosystem model, an improved version of the earlier eco-hydrodynamics model developed for Lake Tanganyika, was used to estimate the annual primary production of Lake Tanganyika and its spatial and temporal variability. The simulations were driven with the National Centres for Environmental Protection (NCEP) records for winds and solar radiation forcing.
2. The simulated annual cycles of the four ecosystem variables and the daily net primary production were compared with the observations. The comparison showed that simulations reproduced realistically the general features of the annual cycles of epilimnial phosphate, net primary production and plankton dynamics.
3. The climatic simulations for the years 1970–2006 yielded a daily averaged integrated upper layer net production ranging from 0.11 to 1.78 g C m⁻² day⁻¹ and daily averaged chlorophyll- a (chl- a ) from 0.16 to 4.3 mg m⁻³. Although the nutrient concentrations in the epilimnion during the strong wind years were high, the net production was low, which is partly because of the greater vertical mixing, produced by strong winds, exposing the phytoplankton to low light conditions in deeper waters. The simulated annual net production and chl- a agreed quite well with observed production available in the literature.
4. We envisage using this model to predict the future scenarios of primary productivity in the lake.     

Spatial and temporal patterns of microcrustacean assemblage structure and secondary production in a wetland ecosystem

1. In contrast to extensive studies of zooplankton in lakes, the role of microcrustaceans in wetlands is not well studied. In this study, spatial and temporal patterns of microcrustacean assemblage structure and secondary production were quantified over a 2-year period in a southeastern U.S.A. wetland.
2. Thirty-two species, including 19 cladocerans, 10 copepods and three ostracods, generated different temporal patterns of density and production between vegetated ( Nymphaea ) and non-vegetated (open-water) zones reflecting species-specific differences in life histories.
3. Summer assemblages were dominated by small, planktonic filter-feeders, typified by high annual production/biomass ( P / B ) and daily production. In contrast, winter assemblages were dominated by larger, epibenthic detritivores with low P / B and high biomass. Seasonal shifts in the relative importance of planktonic species in the warmer months to benthic and epiphytic species in the cooler months suggest that energy flow pathways through microcrustaceans may vary seasonally.
4. Total annual production was higher during both years in the Nymphaea zone (13.0 g and 13.6 g DM m⁻² year⁻¹) than the open-water (8.2 and 6.3 g DM m⁻² year⁻¹), and was similar between years for the entire wetland pond (12.3 and 12.2 g DM m⁻² year⁻¹).
5. Although wetland ecosystems have been the subject of considerable ecological research in the past 20 years, our study is one of the few to demonstrate a highly diverse and relatively productive microcrustacean assemblage. Such comprehensive production studies can be used to quantify the ecological importance of microcrustaceans in freshwater wetland ecosystems.     

Spatio-temporal variation in macroinvertebrate assemblages of glacial streams in the Swiss Alps

1. Changes in water chemistry, benthic organic matter (BOM), and macroinvertebrates were examined in four different glacial streams over an annual cycle. The streams experienced strong seasonal changes in water chemistry that reflected temporal changes in the influence from the source glacier, especially in water turbidity, particulate phosphorus and conductivity.
2. Nitrogen concentrations were high (nitrate-N values were 130–274 μg L^–1), especially during spring snowmelt runoff. Benthic organic matter attained >600 g m^–2 dry mass at certain times, peaks being associated with seasonal blooms of the alga Hydrurus foetidus .
3. Macroinvertebrate taxon richness was two to three times higher (also numbers and biomass) in winter than summer suggesting winter may be a more favourable period for these animals. Benthic densities averaged 1140–3820 ind. m^–2, although peaking as high as 9000 ind. m^–2. Average annual biomass ranged from 102 to 721 mg m^–2, and reached >2000 mg m^–2 at one site in autumn.
4. Taxa common to all sites included the dipterans Diamesa spp. and Rhypholophus sp., the plecopterans Leuctra spp. and Rhabdiopteryx alpina , and the ephemeropterans Baetis alpinus and Rhithrogena spp. Principal components analysis clearly separated winter assemblages from those found in summer.     

Temporal dynamics in epipelic, pelagic and epiphytic algal production in a clear and a turbid shallow lake

SUMMARY 1. Pelagic and epipelic microalgal production were measured over a year in a pre-defined area (depth 0.5 m) in each of two lakes, one turbid and one with clear water. Further estimates of epiphytic production within reed stands were obtained by measuring production of periphyton developed on artificial substrata.
2. Total annual production of phytoplankton and epipelon was 34% greater in the turbid lake (190 g C m⁻² year⁻¹) than in the clearwater lake (141 g C m⁻² year⁻¹). However, the ratio of total production to mean water column TP concentration was two fold greater in the clearwater lake.
3. Phytoplankton accounted for the majority of the annual production (96%) in the turbid lake, while epipelic microalgal production dominated (77%) in the clear lake. The relative contribution of epipelic algae varied over the year, however, and in the turbid lake was higher in winter (11–25%), when the water was relatively clear, than during summer (0.7–1.7%), when the water was more turbid. In the clearwater lake, the relative contribution of epipelon was high both in winter, when the water was most clear, and in mid-summer, when phytoplankton production was constrained either by nutrients or grazing.
4. Compared with pelagic and epipelic primary production, epiphytic production within a reed stand was low and did not vary significantly between the lakes.
5. The study supports the theory of a competitive and compensatory trade-off between primary producers in lakes with contrasting nutrient concentrations, resulting in relatively small differences in overall production between clear and turbid lakes when integrating over the season and over different habitats.     

Problems with the use of terracotta clay saucers as phosphorus-diffusing substrata to assess nutrient limitation of epilithic algae

1. We examined the diffusion properties of terracotta clay saucers, of types often used as components in phosphorus-diffusing substrata for investigating nutrient limitation of epilithic algae.
2. Laboratory experiments showed that phosphorus diffusion was low and inconsistent (0.06–2.6 mg P day⁻¹) through clay saucers filled with agar containing orthophosphate. Similarly, in situ release of phosphorus from two types of terracotta clay saucers (Australian and Italian) was variable (2–8 mg P day⁻¹; 5–25%) under three flow regimes (0, 0.1 and 0.3 m s⁻¹) over 30 days, with most phosphorus being released during the first day. Clogging of pores by agar appears to prevent the diffusion of phosphorus through the terracotta clay saucers. However, the two types of terracotta clay saucer also irreversibly sorbed large quantities of phosphorus (40–140 mg P).
3. Individual saucers can have markedly different physical and chemical properties both within and among terracotta types. Terracotta saucers also contain large quantities of iron, calcium and aluminium that are known binding agents for phosphorus. Variability in saucer composition and diffusion properties makes treatments difficult to replicate and weakens comparisons between studies that have used terracotta with different clay composition and manufacture.
4. We recommend that phosphorus-diffusing substrata should not be constructed from terracotta clay components.     

Estimation of the in-situ settling velocity of particles in lakes using a time series sediment trap

SUMMARY 1. We outline a simple method for estimating the in-situ settling velocity of particles in wind disturbed lakes. The total sedimentation rate (primary and secondary sedimentation) was measured during storm events in two lakes using a time series sediment trap.
2. The sediment trap had 12 sample bottles which were programmed to open for periods of 3–4 h, giving total deployment times ranging from 36 to 48 h.
3. Wave mixed layer theory was used to infer if and when sediment resuspension occurred and the first order rate equation was used to estimate the settling velocity of resuspended particles.
4. The settling velocity during three resuspension events in Lough Neagh and one in Lough Macnean were 34.3, 29.5, 24.1 and 77.3 m day⁻¹, respectively. These are similar to values obtained using recent in-situ video imaging in marine environments, but much larger than previous lake sediment trap studies.     

Continuous growth of the giant grass Zizaniopsis bonariensis in subtropical wetlands

1.  Zizaniopsis bonariensis (giant grass) is an emergent macrophyte species endemic to subtropical wetlands in Brazil, Argentina, Paraguay and Uruguay. In this study, we show the effects of its continuous clonal reproduction and its 'phalanx' growth strategy in the Taim Wetland (southern Brazil).
2. The continuous clonal growth of this 3-m high grass gave rise to the emergence of 11.7 new shoots m⁻² per month and a high total above ground production (2870 g dry weight m⁻² year⁻¹). The biomass of the new shoots emerging every month formed a wave of growth, moderated by only weak seasonal variation.
3. We show its phalanx growth strategy by analysing the variations in population density and shoot height within a transect through the stand canopy. The inverse relation between density and height from the border to the interior indicates self-regulation of biomass.
4. The plants modified their environment, enhancing resistance to drought within the stand and thus facilitating their dominance. This positive feedback suggests that the dominance of the plant might constitute an alternative state in subtropical wetlands.     

Holocene carbon burial by lakes in SW Greenland

The role of the Arctic in future global change processes is predicted to be important because of the large carbon (C) stocks contained in frozen soils and peatlands. Lakes are an important component of arctic landscapes although their role in storing C is not well prescribed. The area around Kangerlussuaq, SW Greenland (66–68°N, 49–54°W) has extremely high lake density, with ∼20 000 lakes that cover about 14% of the land area. C accumulation rates and standing stock (kg C m⁻²), representing late- to mid-Holocene C burial, were calculated from AMS ¹⁴C-dated sediment cores from 11 lakes. Lake ages range from ∼10 000 cal yr  bp to ∼5400 cal yr  bp , and reflect the withdrawal of the ice sheet from west to east. Total standing stock of C accumulated in the studied lakes for the last ∼8000 years ranged from 28 to 71 kg C m⁻², (mean: ∼42 kg C m⁻²). These standing stock determinations yield organic C accumulation rates of 3.5–11.5 g C m⁻² yr⁻¹ (mean: ∼6 g C m⁻² yr⁻¹) for the last 4500 years. Mean C accumulation rates are not different for the periods 8–4.5 and 4.5–0 ka, despite cooling trends associated with the neoglacial period after 4.5 ka. We used the mean C standing stock to estimate the total C pool in small lakes (<100 ha) of the Kangerlussuaq region to be ∼4.9 × 10¹³ g C. This C stock is about half of that estimated for the soil pool in this region (but in 5% of the land area) and indicates the importance of incorporating lakes into models of regional C balance at high latitudes.     

Near-zero recent carbon accumulation in a bog with high nitrogen deposition in SW Sweden

We present data on the accumulation of carbon and nitrogen into an open oceanic ombrotrophic bog, SW Sweden, with high levels of anthropogenic nitrogen deposition. The aim was to investigate if this peatland currently acts as a sink for atmospheric carbon. Peat cores were sampled from the top peat layer in five different vegetation types. Small pines were used to date the cores. The cores bulk density and carbon and nitrogen content were determined. A vegetation-classified satellite image was used to estimate the areal extent of the vegetation types and to scale up these results to bog level. The rate of current carbon input into the upper oxic acrotelm was 290 g m⁻² yr⁻¹, and there were no significant differences in accumulation rates among the vegetation types. This organic matter input to the acrotelm was almost completely decomposed before it was deposited for storage in the deeper peat layers (the catotelm) and only a small fraction (≪1%) or 0.012 g m⁻² yr⁻¹ of the carbon would be left, assuming a residence time of 100 years in the acrotelm. Nitrogen accumulation rates differed between the vegetation classes, and the average input via primary production varied from 5.33 to 16.8 g m⁻² yr⁻¹. Current nitrogen input rates into the catotelm are much lower, 0–0.059 g m⁻² yr⁻¹, with the highest accumulation rates in lawn-dominated communities. We suggest that one of the main causes of the low carbon input rates is the high level of nitrogen deposition, which enhances decomposition and changes the vegetation from peat-forming Sphagnum -dominance to dominance by dwarf shrubs and graminoids.     

Change in CO2 balance under a series of forestry activities in a cool-temperate mixed forest with dense undergrowth

To evaluate the effects on CO₂ exchange of clearcutting a mixed forest and replacing it with a plantation, 4.5 years of continuous eddy covariance measurements of CO₂ fluxes and soil respiration measurements were conducted in a conifer-broadleaf mixed forest in Hokkaido, Japan. The mixed forest was a weak carbon sink (net ecosystem exchange, −44 g C m⁻² yr⁻¹), and it became a large carbon source (569 g C m⁻² yr⁻¹) after clearcutting. However, the large emission in the harvest year rapidly decreased in the following 2 years (495 and 153 g C m⁻² yr⁻¹, respectively) as the gross primary production (GPP) increased, while the total ecosystem respiration (RE) remained relatively stable. The rapid increase in GPP was attributed to an increase in biomass and photosynthetic activity of Sasa dwarf bamboo, an understory species. Soil respiration increased in the 3 years following clearcutting, in the first year mainly owing to the change in the gap ratio of the forest, and in the following years because of increased root respiration by the bamboo. The ratio of soil respiration to RE increased from 44% in the forest to nearly 100% after clearcutting, and aboveground parts of the vegetation contributed little to the RE although the respiration chamber measurements showed heterogeneous soil condition after clearcutting.     

Does nitrogen deposition affect soil microfungal diversity and soil N and P dynamics in a high Arctic ecosystem?

In a high Arctic polar semidesert ecosystem (ambient N deposition c. 0.1 g N m⁻² a⁻¹), the effects of N enrichment on the diversity of soil microfungi and on N content and availability in organic and mineral soils were determined. Three N (total: 0, 0.5, 5 g N m⁻² a⁻¹) and two P (total 0, 1 g m⁻² a⁻¹) treatments were applied, since P may limit response to N in this ecosystem. Organic and mineral soils were sampled in June and August in the second year of treatment for microfungi, pH, moisture content, and total N and P. In the third year, soils were resampled for extractable and total N and P. The fungi isolated were typical of high pH soils in the High Arctic and Antarctic. The species richness and diversity of soil microfungi were very low, with ranges as follows: Shannon diversity, 0.56–1.5; richness, 2–6; evenness, 0.79–0.9. There was no significant effect of treatment on the frequency of occurrence of different taxa of soil microfungi. Time of sampling also had no significant impact on fungal assemblages, although different, more diverse communities were isolated from organic, rather than mineral, soils. Nitrate-N in organic soil decreased significantly when P was added alone, but not when P and N were added together. Addition of 0.5 g N m⁻² a⁻¹, a rate deposition already occurring in Greenland and Iceland, appeared to exceed N demand even when P limitation was relieved. There was no apparent soil acidification as a result of the N treatments.     

Impact of sediment and nutrient inputs on growth and survival of tadpoles of the Western Toad

1. Sediment and nutrient loading in freshwater systems are leading causes of aquatic habitat degradation globally. We investigated the impacts of fine-sediment and nutrient additions on the growth and survival of western toad ( Bufo boreas ) tadpoles and emergent metamorphs in mesocosm and exclosure experiments.
2. Mesocosm tanks received weekly pulses of fine sediments to create initial concentrations of 0, 130 and 260 mg L⁻¹ of suspended sediment and either bi-weekly additions of nutrients (N = 160 μg L⁻¹, P = 10 μg L⁻¹) or no additions in a factorial design. Within mesocosms, tadpole exclosures allowed for quantification of tadpole grazing pressure on periphyton biomass, chlorophyll- a and sediment deposition.
3. Tadpoles receiving sediment additions experienced slower growth rates and reduced survival to metamorphosis, although no effects of treatment were detected on size at metamorphosis or time to metamorphosis. Nutrient additions also lowered survival, but had no impact on other measured parameters of tadpole fitness. Dissections and gut content analysis revealed that tadpoles ingested sediment in large quantities altering the proportion of the organic content of ingested food.
4. Together these results suggest that although sediment was readily consumed by tadpoles, its presence in the larval environment had an overall negative effect on tadpole growth and survival, although not as severe as predicted.     

Very high secondary production at a lake outlet

SUMMARY. 1. Larvae and pupae of Simulium noelleri Fried, coated the concrete of parts of an artificial lake outlet in southern England.
2. In the first two (of three) summer generations, development was synchronous and this allowed the calculation of their secondary production by the instantaneous growth method. The production of the two summer generations was, respectively, 229.1 g C m⁻² (7.4 g C m⁻²day⁻¹) and 185.5 g C m⁻² (8.8 g C m⁻² day⁻¹) The contribution of the third summer generation, and the overwintering generation, to annual production would be less than that of the first two summer generations. Nevertheless, annual production will have exceeded 500 g C m⁻² at this site.
3. Larvae are suspension feeders and they captured the rich supply of particulate and dissolved organic material which passed over them after export from the lake. As food is brought to the larvae they only require space for attachment and can thus build up very high population densities (which exceeded 1 × 10⁶ m⁻² on some occasions during the summer). The high population densities result in a high biomass and hence in the high levels of production.     

Lough Ennell: laboratory studies on sediment phosphorus release under varying mixing, aerobic and anaerobic conditions

SUMMARY. 1. The exchange of phosphorus between the epilimnetic (shallow zone) sediment and water column in Lough Ennell was investigated in laboratory experiments using five intact cores.
2. Variations in water mixing, sediment suspension and aerobic–anaerobic oxygen status in the water column and its effects on sediment phosphorus release rates were determined.
3. Experimental results indicated that phosphorus release is possible under both aerobic and anaerobic conditions. Aerobic release (0.025 mg P l⁻¹ over 5 days) was possible up to the point when mass resuspension of sediment occurred. Anaerobic release for the same period and mixing conditions was 0.183 mg Pl⁻¹.
4. The release rate under aerobic conditions at 10°C equates to an internal areal loading of 0.134 g P m⁻² yr^−1, which is approximately 17% and 30% of the average total phosphorus and orthophosphate loadings respectively for the period 1974–79.
5. The results clearly implicate aerobic inorganic phosphorus release from the epilimnetic sediments as a significant source of this nutrient to the overlying water column and is likely a major factor in the continuing eutrophic status in the lake.     

High organic loading influences the physical characteristics of aerobic sludge granules

Aims:  The effect of high organic loading rate (OLR) on the physical characteristics of aerobic granules was studied.
Methods and Results:  Two column-type sequential aerobic sludge blanket reactors were fed with either glucose or acetate as the main carbon source, and the OLR was gradually raised from 6 to 9, 12 and 15 kg chemical oxygen demand (COD) m⁻³ d⁻¹. Glucose-fed granules could sustain the maximum OLR tested. At a low OLR, these granules exhibited a loose fluffy morphology dominated by filamentous bacteria. At higher OLRs, these granules became irregularly shaped, with folds, crevices and depressions. In contrast, acetate-fed granules had a compact spherical morphology at OLRs of 6 and 9 kg COD m⁻³ d⁻¹, with better settling and strength characteristics than glucose-fed granules at similar OLRs. However, acetate-fed granules could not sustain high OLRs and disintegrated when the OLR reached 9 kg COD m⁻³ d⁻¹.
Conclusions:  The compact regular microstructure of the acetate-fed granules appeared to limit mass transfer of nutrients at an OLR of 9 kg COD m⁻³ d⁻¹. The looser filamentous microstructure of the glucose-fed granules and the subsequent irregular morphology delayed the onset of diffusion limitation and allowed significantly higher OLRs to be attained.
Significance and Impact of the Study:  High organic loading rates are possible with aerobic granules. This research would be helpful in the development of aerobic granule-based systems for high-strength wastewaters.     

Effects of artificial ultraviolet-B radiation on experimental aquatic microcosms

1. The effects of prolonged ultraviolet-B (UVB) radiation on freshwater communities were studied in indoor microcosms (600 L) with artificial light sources, simulating a clear, shallow, mesotrophic aquatic ecosystem. A range of six intensities (in duplicate) of UVB radiation, ranging from 0 (control) to 9.56 kJ m⁻² day⁻¹ at the water surface, was applied for 8 weeks. The UVB radiation levels, attenuation, shading and scattering were comparable to those in Dutch shallow freshwater systems. Physical, chemical and biological variables were monitored weekly.
2. The UVB treatment did not affect the abundance, species composition or biovolume of the phytoplankton or zooplankton communities, nor did it affect the periphyton or the macroinvertebrate community. A few species showed a significant response on some of the sampling dates, but there was no negative UVB effect at the community level. Overall, the ecosystems in the microcosms were not affected by the UVB treatment.
3. In a bio-assay, a laboratory clone of Daphnia pulex , not subjected to UVB radiation, was fed with seston from the microcosms. Daphnia pulex feeding on seston from the control microcosms grew faster, had better survival and better reproduction than D. pulex feeding on seston from the UVB treated microcosms. The phytoplankton–zooplankton interaction may have been influenced by the UVB treatment.
4. The dissolved oxygen content (DOC) concentrations in the microcosms were around 5 mg L^−1. The DOC levels in Dutch systems rarely fall below 10 mg L^−1. This might provide sufficient protection against the detrimental effects of increased UVB radiation.