共查询到20条相似文献,搜索用时 0 毫秒
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Andrea Musacchio 《Current biology : CB》2010,20(15):R634-R637
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《Cell cycle (Georgetown, Tex.)》2013,12(20):2310-2313
Post-translational modification by SUMO is a dynamic and reversible process and several SUMO-specific proteases that remove SUMO from substrates have been identified. We have recently described the activities of a new SUMO-specific protease, SENP5. We found that SENP5 discriminates between SUMO-1 and SUMO-2/3 and cells depleted of SENP5 by RNAi failed to proliferate. Our findings support the idea that differential substrate selection by the mammalian SUMO-specific proteases underlies their regulation of distinct biological processes. Furthermore, our finding of a non-redundant function for SENP5 in cell proliferation provides further support for the model that, analogous to phosphorylation, cycles of SUMOylation and deSUMOylation regulate orderly progression through cell division. 相似文献
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Evelyn B. Voura Jane L. English Hoi-Ying E. Yu Andrew T. Ho Patrick Subarsky Richard P. Hill Carlo V. Hojilla Rama Khokha 《PloS one》2013,8(10)
To test if proteolysis is involved in tumor cell extravasation, we developed an in vitro model where tumor cells cross an endothelial monolayer cultured on a basement membrane. Using this model we classified the ability of the cells to transmigrate through the endothelial cell barrier onto the underlying matrix, and scored this invasion according to the stage of passage through the endothelium. Metalloproteinase inhibitors reduced tumor cell extravasation by at least 35%. Visualization of protease and cell adhesion molecules by confocal microscopy demonstrated the cell surface localization of MMP-2, MMP-9, MT1-MMP, furin, CD44 and αvβ3, during the process of transendothelial migration. By the addition of inhibitors and bio-modulators we assessed the functional requirement of the aforementioned molecules for efficient migration. Proteolytic digestion occurred at the cell-matrix interface and was most evident during the migratory stage. All of the inhibitors and biomodulators affected the transition of the tumor cells into the migratory stage, highlighting the most prevalent use of proteolysis at this particular step of tumor cell extravasation. These data suggest that a proteolytic interface operates at the tumor cell surface within the tumor-endothelial cell microenvironment. 相似文献
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检查点:细胞周期的质量监督 总被引:3,自引:0,他引:3
细胞周期的最主要任务是将其基因组DNA在DNA合成期(S期)完整地复制成两份拷贝,而后在分裂期(M期)将这两份拷贝正确无误地分配给两个子代细胞。如果在这一过程中产生错误,又得不到及时纠正,那么将导致基因组的不稳定和变异。对单细胞生物,其后果是导致细胞... 相似文献
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Polo样激酶1在细胞周期及细胞周期监测点中的功能 总被引:1,自引:0,他引:1
Plk1(Polo-like kinase 1)是一类从酵母到人类都高度保守的丝氨酸/苏氨酸蛋白激酶,是真核细胞有丝分裂的重要调控因子.Plk1随有丝分裂进程定位于不同位点,调节分裂期进入、纺锤体形成和胞质分裂等过程.Plk1能够与磷酸化的停靠蛋白结合,从而在不同空间被激活以满足其在细胞周期中的不同功能.Plk1还参与G2和M期DNA损伤监测点的调节,对于DNA损伤恢复后重新进入有丝分裂期是必须的.目前,Plk1的重要功能尤其是在DNA损伤监测点中发挥的重要功能正在被广泛研究.Plk1在多种恶性肿瘤中存在过表达且与肿瘤发生密切相关,对于Plk1功能的深入研究为以Plk1为靶的肿瘤治疗提供理论依据 相似文献
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《Fly》2013,7(3):133-137
Drosophila researchers met in sunny San Diego for the 49th annual meeting of The Genetics Society of America. It was cold outside and even colder inside. Like last year, ‘Mitosis, Meiosis and Cell Division’ was no longer a session. Instead, we searched out and covered talks and posters in ‘Cell Division and Growth Control’, ‘Gametogenesis’, ‘Cytoskeleton and Cell Biology’ and ‘Genome and Chromosome Structure’. We split up for maximal coverage and re-grouped later for the Workshop on Cell Cycle and Checkpoints. We apologize in advance for the brevity or omission of some reports. 相似文献
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《Fly》2013,7(4):286-289
Although the molecular elements controlling cell cycle progression are well established, the mechanisms regulating how cell proliferation is triggered in response to extrinsic stimuli and how cell divisions change speed, particularly in stem or tumor cells or regenerative tissues, are poorly understood. One exceptional model system in which these events are precisely defined is Drosophila abdominal morphogenesis, in which stem-like histoblasts build the adult epidermis at metamorphosis by undergoing a series of sequential transitions from a non-proliferative to a growing, and finally to an invasive epithelium. We have recently uncovered in histoblasts an internal logic modulating cell cycle transitions that should constitute a reference paradigm for the study of other equivalent processes in stem cell, cancer or developmental biology. 相似文献