共查询到20条相似文献,搜索用时 46 毫秒
1.
We describe a novel thermosensitive escape behavior in Drosophila larvae and a simple assay to accurately define the response temperature. When a larva is placed in a droplet of water that is subsequently heated, a stereotypical escape response is robustly elicited at 29°C. Larvae defective for the painless TRP receptor, or blocked in the function of class IV multi-dendritic sensory dendrites respond to this stimulus at reproducibly higher temperature (34°C). The escape response has novel behavioral components and a lower temperature threshold in comparison with the responses to touch with a hot needle. Furthermore the assay minimizes operator bias that is present in current tests of thermosensitive nociception and generates a precise determination of temperature at the point of response. This response is highly reproducible and directly applicable to genetic and neural circuit analysis of a simple escape behavior. 相似文献
2.
Julie Augustin Guy Boivin Jacques Brodeur Gaétan Bourgeois 《Ecological Entomology》2020,45(3):741-750
1. This study investigated the effect of temperatures ranging from 10.8 to 34.2 °C on seven walking parameters of an egg parasitoid, Anaphes listronoti. Those responses were compared with a theoretical kinetic model in order to disentangle the kinetic response of the insect from its integrated response. 2. Walking speed increased continuously with temperature, but walking distance and duration were maximised between 25 and 30 °C, and decreased at higher and lower temperatures. At the lowest temperature, females unexpectedly walked a greater distance and for a longer duration in comparison with intermediate temperatures. 3. The number of walking bouts followed the same polynomial trend as walking distance and duration, with a maximum between 30 and 35 °C. The duration of walking bouts was maximised at 20 °C and decreased at lower and higher temperatures, whereas the duration of resting bouts linearly decreased with increasing temperature. There was no effect of temperature on the turning rate of females. 4. For A. listronoti, walking speed and duration of resting bouts followed the kinetic response, but the other behavioural components did not, especially at the lowest and highest temperatures. Walking distance and duration were higher than expected under the kinetic response at 10.8 °C and lower than expected at 34.2 °C. This pattern suggests an integrated response combining behavioural escape from adverse temperature and energy saving. 5. This detailed analysis of the walking behaviour of A. listronoti illustrates the complexity of insect behavioural responses to temperature and the difficulty involved in assessing underlying mechanisms. 相似文献
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I. Cornet E. Van Derlinden A.M. Cappuyns J.F. Van Impe 《Letters in applied microbiology》2010,51(4):450-455
Aims: When subjected to dynamic temperatures surpassing the expected maximum growth temperature, Escherichia coli K12 MG1655 shows disturbed growth curves. These irregular population dynamics were explained by considering two subpopulations, i.e. a thermoresistant and a thermosensitive one ( Van Derlinden et al. 2010a ). In this paper, the influence of the initial cell concentration on the subpopulations’ dynamics is evaluated. Methods and Results: Experiments were performed in a bioreactor with the temperature increasing from 42 to 65·2°C (1 and 4°C h?1) with varying initial cell concentrations [6, 12 and 18 ln(CFU ml?1)]. When started from the highest cell concentration, the population was characterized by a higher overall maximum growth temperature and a higher inactivation temperature. For all experimental set‐ups, resistant cells were still growing at the final temperature of 65·2°C. Conclusions: The initial cell concentration had no effect on temperature resistance. The increase in temperature resistance of the sensitive subpopulation was because of the change of the physiological state to the stationary phase. Significance and Impact of the Study: A higher initial cell concentration leads to higher heat stress adaptation when cultures reach a maximum cell concentration. The observed growth at a temperature of 65·2°C is very important for food safety and the temperature treatment of micro‐organisms. 相似文献
6.
Xiao-Ping Wang Fang-Sen Xue Feng Ge Cheng-Ai Zhou Lan-Shao You 《Physiological Entomology》2004,29(5):419-425
Abstract. The effects of thermoperiods on diapause induction in continuous darkness or under a 12 : 12 h LD photoperiod were investigated in the cabbage beetle, Colaphellus bowringi Baly, a typical short‐day species. The diapause response curves both at different constant temperatures and at the thermocycle of format CT x: (24 ? x) h (16 : 28 °C) under continuously dark rearing conditions showed that the incidence of diapause depended mainly on whether or not the mean temperature was ≤20 °C or >20 °C. If the mean temperature was ≤20 °C, all individuals entered diapause; if >20 °C, the incidence of diapause declined gradually with increasing mean temperatures. The thermocycle (CT 12 : 12 h) with a series of different cryophases (8–22 °C) and thermophases (24–32 °C) under continuous darkness demonstrated a cryophase response threshold temperature of approximately 19 °C and a thermophase response threshold temperature of approximately 31 °C. Thermoperiodic amplitude (temperature difference between cryophase and thermophase) was shown to have a significant influence on diapause induction at the mean temperatures of 22, 23 and 24 °C, but not at ≥25 °C. Thermoperiodic responses under LD 12 : 12 h clearly showed that the incidence of diapause was influenced strongly by the photophase temperature. The thermoperiod under LD 12 : 12 h induced a much lower incidence of diapause than the thermoperiod with the same temperature in continuous darkness. The ecological significance of thermoperiodic induction of diapause in this species is discussed. 相似文献
7.
The Acclimation of European Sea Bass (Dicentrarchus labrax) to Temperature: Behavioural and Neurochemical Responses
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Amanda Tedesco Stefano Malavasi Enrico Alleva Carla Cioni 《Ethology : formerly Zeitschrift fur Tierpsychologie》2015,121(1):68-83
Studies on fish behavioural and neurophysiological responses to water temperature change may contribute to an improved understanding of the ecological consequences of global warming. We investigated behavioural and neurochemical responses to water temperature in European sea bass (Dicentrarchus labrax) acclimated to three temperatures (18, 22 and 28°C). After 21 d of acclimation, three groups of 25 fish each were exposed to four behavioural challenges (foraging, olfactory, aversive and mirror tests). The expression of choline acetyltransferase (ChAT) was then analysed by Western blotting in CNS homogenates (from a subset of the same fish) as a marker for cholinergic system activity. In both foraging and olfactory tests, fish acclimated to 28°C exhibited significantly higher arousal responses than fish acclimated to lower temperatures. All specimens showed fright behaviour in the aversive test, but the latency of the escape response was significantly less in the fish at 28°C. Finally, the highest mirror responsiveness was exhibited by the fish acclimated to 22°C. As in the case of cholinergic neurotransmission, significantly higher ChAT levels were detected in the telencephalon, diencephalon, cerebellum and spinal cord of fish acclimated to 22 or 28°C in comparison with those maintained at 18°C. Lower ChAT levels were detected in the mesencephalon (optic tectum) at 22 and 28°C than at 18°C. These data indicate that neuronal functions are affected by water temperature. Increases or decreases in ChAT expression can be related to the functional modulation of brain and spinal cord centres involved in behavioural responses to temperature change. Overall, the results of this study suggest that the environmental temperature level influences behaviour and CNS neurochemistry in the European sea bass. 相似文献
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Wannisa Boonlai Vimon Tantishaiyakul Namon Hirun Tanatchaporn Sangfai Krit Suknuntha 《AAPS PharmSciTech》2018,19(5):2103-2117
Thermosensitive hydrogels are of great interest for in situ gelling drug delivery. The thermosensitive vehicle with a gelation temperature in a range of 30–36°C would be convenient to be injected as liquid and transform into gel after injection. To prepare novel hydrogels gelling near body temperature, the gelation temperature of poloxamer 407 (PX) were tailored by mixing PX with poly(acrylic acid) (PAA). The gelation behaviors of PX/PAA systems as well as the interaction mechanism were investigated by tube inversion, viscoelastic, shear viscosity, DSC, SEM, and FTIR studies. The gelation temperature of the plain PX solutions at high concentration of 18, 20, and 22% (w/w) gelled at temperature below 28°C, which is out of the suitable temperature range. Mixing PX with PAA to obtain 18 and 20% (w/w) PX with 1% (w/w) PAA increased the gelation temperature to the desired temperature range of 30–36°C. The intermolecular entanglements and hydrogen bonds between PX and PAA may be responsible for the modulation of the gelation features of PX. The mixtures behaved low viscosity liquid at room temperature with shear thinning behavior enabling their injectability and rapidly gelled at body temperature. The gel strength increased, while the pore size decreased with increasing PX concentration. Metronidazole, an antibiotic used for periodontitis, was incorporated into the matrices, and the drug did not hinder their gelling ability. The gels showed the sustained drug release characteristic. The thermosensitive PX/PAA hydrogel could be a promising injectable in situ gelling system for periodontal drug delivery. 相似文献
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Thermal environments can influence many fitness‐related traits including life span. Here, we assess whether longevity in Drosophila melanogaster can experimentally evolve as a correlated response to cold‐stress selection, and whether genotype‐by‐temperature and sex‐by‐temperature interactions are significant components of variation in life span. Three replicated S lines were cold‐stress selected and compared with their respective unselected controls (Clines) in the 16th generation of thermal selection. Cold‐stress resistance exhibited a substantial direct response to selection, and also showed a significant interaction between sex and type of line. Mean longevity exhibited a significant interaction between adult test temperature (14 and 25 °C) and line (with suggestive evidence for increased longevity of S lines when tested at 14 °C), but there was no evidence for increased longevity in S lines at normal temperatures (i.e. 25 °C). Another temperature‐dependent effect was sex‐specific, with males being the longer lived sex at 25 °C but the less long‐lived sex at 14 °C. Additionally, we tested in an exploratory way the relationship between longevity and cold‐stress resistance by also measuring resistance to a prefreezing temperature before and after one generation of longevity selection at 14 °C (selection intensity, i = 1.47 for S lines, and 1.42 for C lines). In this longevity selection, we found that cold‐stress resistance increased by about 6% in S lines and 18% in C lines. However, taken together, the results indicate no simple relationship between longevity and cold‐stress resistance, with genotype‐by‐sex interactions in both traits. Temperature dependent interaction in longevity is apparent between S and C lines, and sex‐specific variation in mean longevity also depends on temperature. 相似文献
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Patrick Opdensteinen Aleksandr Lobanov Johannes Felix Buyel 《Biotechnology journal》2021,16(4):2000340
Incubation at pH 4.0 or blanching at ∼65°C facilitates the purification of biopharmaceutical proteins from plants by precipitating most of the host cell proteins (HCPs) before chromatography. However, both methods are compatible only with pH or thermostable target proteins whereas many target proteins may irreversibly denature, e.g., at pH < 4.0. Here, we developed a combined pH/temperature treatment for clarified tobacco extracts and intact leaves. The latter were subjected to a blanching procedure, i.e., the submersion into a hot buffer. Using a design of experiments approach we identified conditions that remove ∼70% of HCPs at ∼55°C, using the thermosensitive antibody 2G12 and the pH-sensitive DsRed as model proteins. We found that pH and temperature exerted a combined effect during the precipitation of HCPs in the pH range 5.0–7.0 at 35°C–60°C. For clarified extracts, the temperature required to achieve a DsRed purity threshold of 20% total soluble protein (TSP) increased from 54°C to 63°C when the pH was increased from 6.4 to 7.3. The pH-stable antibody 2G12 was less responsive to the combined treatment, but the purity of 1% TSP was achieved at 35°C instead of 44°C when the pH was reduced from 6.3 to 5.8. When blanching intact leaves, product losses were not exacerbated at pH 4.0. Indeed, the highest DsRed purity (58% TSP) was achieved at this pH, combined with a temperature of 60°C and an incubation time of 30 min. In contrast, the highest 2G12 purity (0.7% TSP) was achieved at pH 5.1 and 40°C with an incubation time of 20 min. Our data suggest that a combined pH/temperature regime can avoid extreme values of either parameter; therefore, broadening the applicability of these simple purification techniques to other recombinant proteins. 相似文献
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Wang DS Zhao SF Zhao MX Li J Chi ZM 《Journal of industrial microbiology & biotechnology》2011,38(9):1545-1552
Highly thermosensitive and permeable mutants are the mutants from which intracellular contents can be released when they are
incubated both in low osmolarity water and at non-permissive temperature (usually 37°C). After mutagenesis by using nitrosoguanidine,
a highly thermosensitive and permeable mutant named A11-b was obtained from Saccharomycopsis fibuligera A11-12, a trehalose overproducer in which the acid protease gene has been disrupted. Of the total trehalose, 73.8% was released
from the mutant cells suspended in distilled water after they had been treated at 37°C overnight. However, only 10.0% of the
total trehalose was released from the cells of S. fibuligera A11-12 treated under the same conditions. The cell volume of the mutant cells suspended in distilled water and treated at
37°C overnight was much bigger than that of S. fibuligera A11-12 treated under the same conditions. The cell growth and trehalose accumulation of the mutant were almost the same as
those of S. fibuligera A11-12 during the cultivation at the flask level and in a 5-l fermentor. Both could accumulate around 28.0% (w/w) trehalose
from cassava starch. After purification, the trehalose crystal from the aqueous extract of the mutant was obtained. 相似文献
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Leonard Nunney Warren Cheung 《Evolution; international journal of organic evolution》1997,51(5):1529-1535
The reaction norm linking rearing temperature and size in Drosophila melanogaster results in progressively larger flies as the temperature is lowered from 30°C to 18°C, but it has remained unclear whether this phenotypic plasticity is part of an adaptive response to temperature. We found that female D. melanogaster reared to adulthood at 18°C versus 25°C showed a 12% increase in dry weight. Measurements of the fecundity of these two types of fly showed that the size change had no effect on lifetime fecundity, regardless of the adult test temperature. Thus the phenotypic plasticity breaks the usual positive correlation between body size and fecundity. However, at a given temperature, early fecundity (defined as productivity for days 5 through 12 after eclosion at 25°C and days 7 through 17 at 18°C) was highest when the rearing and test temperatures were the same. The early fecundity advantage due to rearing at the test temperature was 25% at 18°C and 16% at 25°C, a result consistent with the overall phenotypic response to temperature being adaptive. This conclusion is further supported by the finding that the temperature treatments resulted in a trade-off between early fecundity and longevity, a trade-off that parallels the known genetic correlation. Another parallel is that both the temperature-induced and genetic effects are independent of total fecundity. By contrast, within the temperature treatments, the phenotypic correlation between early fecundity and longevity was positive, illustrating the danger of assuming that phenotypic and genetic correlations are similar, or even of the same sign. 相似文献
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The mitochondria of intertidal invertebrates continue to function when organisms are exposed to rapid substantial shifts in
temperature. To test if mitochondrial physiology of the clam Mercenaria mercenaria is compromised under elevated temperatures, we measured mitochondrial respiration efficiency at 15°C, 18°C, and 21°C using
a novel, high-throughput, microplate respirometry methodology developed for this study. Though phosphorylating (state 3) and
resting (state 4) respiration rates were unaffected over this temperature range, respiratory control ratios (RCRs: ratio of
state 3 to state 4 respiration rates) decreased significantly above 18°C (p < 0.05). The drop in RCR was not associated with reduction of phosphorylation efficiency, suggesting that, while aerobic
scope of mitochondrial respiration is limited at elevated temperatures, mitochondria continue to efficiently produce adenosine
triphosphate. We further investigated the response of clam mitochondria to elevated temperatures by monitoring phosphorylation
of mitochondrial protein. Three proteins clearly demonstrated significant time- and temperature-specific phosphorylation patterns.
The protein-specific patterns of phosphorylation may suggest that a suite of protein kinases and phosphatases regulate mitochondrial
physiology in response to temperature. Thus, while aerobic scope of clam mitochondrial respiration is reduced at moderate
temperatures, specific protein phosphorylation responses reflect large shifts in function that are initiated within the organelle
at higher temperatures. 相似文献
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The question as to the role that genes play in determining life-span is essentially unresolved. Although it is well documented that genotype influences longevity, this is no way demonstrates that life-span is genetically determined. In the present study we examine five temperature-sensitive mutations for their effect on the aging process. At the permissive temperature (22°C ), the longevity of each mutant strain is comparable to that of wild type. However, at the restrictive temperature (29°C ) the life-span of these mutants is severely curtailed. Using behavior loss as a landmark of adult physiological age, we examined each of these strains for its pattern of behavior loss relative to longevity, and compared each to a wild-type strain. In four of the mutations the pattern of behavior loss relative to longevity was severely altered at one or both temperatures. However, one strain, adl-16tsl displayed a pattern of behavior loss that was indistinguishable from wild type at both 22°C and 29°C. At 29°C not only was the longevity decreased, the pattern of behavior loss was also compressed into a shorter time period. The compression of the pattern of behavior loss was proportional to the reduction in life-span. Thus it appears that this mutation, adl-16tsl, may accelerate the normal aging process when placed at 29°C. The potential utility of these types of mutants for studying the aging process is discussed. 相似文献
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Clarisse Perrin Christiane Guimont Patrice Bracquart Jean-Luc Gaillard 《Current microbiology》1999,39(6):342-347
Streptococcus thermophilus is widely used in food fermentations; it commonly suffers diverse stress challenges during manufacturing. This study investigated
the cold shock response of S. thermophilus when the cell culture temperature shifted from 42°C to 15°C or 20°C. The growth of cells was affected more drastically after
cold shock at 15°C than at 20°C. The generation time was increased by a factor of 19 when the temperature was lowered from
42° to 20°C, and by a factor of 72 after a cold shock at 15°C. The two-dimensional electrophoretic protein patterns of S. thermophilus under cold shock conditions were compared with the reference protein pattern when cells were grown at optimal temperature.
Two proteins of 21.5 and 7.5 kDa synthesized in response to cold shock were characterized. N-terminal sequencing and sequence
homology searches have shown that the 7.5-kDa protein belonged to the family of the major cold shock proteins, while no homology
was found for the new cold shock protein of 21.5 kDa.
Received: 4 June 1999 / Accepted: 6 July 1999 相似文献
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Yang Tang Sijia Lu Chao Fang Huan Liu Lidong Dong Haiyang Li Tong Su Shichen Li Lingshuang Wang Qun Cheng Baohui Liu Xiaoya Lin Fanjiang Kong 《Plant biotechnology journal》2023,21(4):782-791
Flowering time is one of important agronomic traits determining the crop yield and affected by high temperature. When facing high ambient temperature, plants often initiate early flowering as an adaptive strategy to escape the stress and ensure successful reproduction. However, here we find opposing ways in the short-day crop soybean to respond to different levels of high temperatures, in which flowering accelerates when temperature changes from 25 to 30 °C, but delays when temperature reaches 35 °C under short day. phyA-E1, possibly photoperiodic pathway, is crucial for 35 °C-mediated late flowering, however, does not contribute to promoting flowering at 30 °C. 30 °C-induced up-regulation of FT2a and FT5a leads to early flowering, independent of E1. Therefore, distinct responsive mechanisms are adopted by soybean when facing different levels of high temperatures for successful flowering and reproduction. 相似文献
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Increasing temperature decreases the predatory effect of the intertidal shanny Lipophrys pholis on an amphipod prey
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Interactions between Lipophrys pholis and its amphipod prey Echinogammarus marinus were used to investigate the effect of changing water temperatures, comparing current and predicted mean summer temperatures. Contrary to expectations, predator attack rates significantly decreased with increasing temperature. Handling times were significantly longer at 19° C than at 17 and 15° C and the maximum feeding estimate was significantly lower at 19° C than at 17° C. Functional‐response type changed from a destabilizing type II to the more stabilizing type III with a temperature increase to 19° C. This suggests that a temperature increase can mediate refuge for prey at low densities. Predatory pressure by teleosts may be dampened by a large increase in temperature (here from 15 to 19° C), but a short‐term and smaller temperature increase (to 17° C) may increase destabilizing resource consumption due to high maximum feeding rates; this has implications for the stability of important intertidal ecosystems during warming events. 相似文献
19.
Chun-Hai Zhao Tong Zhang Zhen-Ming Chi Zhe Chi Jing Li Xiang-Hong Wang 《Bioprocess and biosystems engineering》2010,33(5):549-556
The intracellular protein in the highly thermosensitive and permeable mutant can be easily released when they are incubated
both in the low-osmolarity water and at the non-permissive temperature (usually 37 °C). After the mutant was grown in the
yacon extract for 45 h, the crude protein content in the highly thermosensitive and permeable mutant Z114 was 59.1% and over
61% of the total protein could be released from the cells treated at 37 °C. The mutant cells grown in the yacon extract still
contained high level of essential amino acids and other nutrients. This means that the yacon extract could be used as the
medium for growth of the highly thermosensitive and permeable mutant which contained high content of crude protein. 相似文献
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THOMAS LENNÉ GARY BRYANT CHARLES H. HOCART CHENG X. HUANG MARILYN C. BALL 《Plant, cell & environment》2010,33(10):1731-1741
Using cryo‐SEM with EDX fundamental structural and mechanical properties of the moss Ceratodon purpureus (Hedw.) Brid. were studied in relation to tolerance of freezing temperatures. In contrast to more complex plants, no ice accumulated within the moss during the freezing event. External ice induced desiccation with the response being a function of cell type; water‐filled hydroid cells cavitated and were embolized at ?4 °C while parenchyma cells of the inner cortex exhibited cytorrhysis, decreasing to ~20% of their original volume at a nadir temperature of ?20 °C. Chlorophyll fluorescence showed that these winter acclimated mosses displayed no evidence of damage after thawing from ?20 °C while GCMS showed that sugar concentrations were not sufficient to confer this level of freezing tolerance. In addition, differential scanning calorimetry showed internal ice nucleation occurred in hydrated moss at ~?12 °C while desiccated moss showed no evidence of freezing with lowering of nadir temperature to ?20 °C. Therefore the rapid dehydration of the moss provides an elegantly simple solution to the problem of freezing; remove that which freezes. 相似文献