共查询到20条相似文献,搜索用时 15 毫秒
1.
Winifred U. Anake Nsikak U. Benson Imokhai Theophilus Tenebe PraiseGod Chidozie Emenike Godson R. E. E Ana Suojiang Zhang 《人类与生态风险评估》2020,26(1):242-254
AbstractQuantification of PM2.5 (particulate matter <2.5?µm) bound heavy metals and their potential health risks were carried out around a cement manufacturing company in Ewekoro, Nigeria. The PM2.5 samples were collected using Environtech gravimetric sampler. A four-staged sequential extraction procedure was used to fractionate PM2.5 bound chromium (Cr), lead (Pb), aluminum (Al), copper (Cu), and silver (Ag), and further analyzed using inductively coupled plasma mass spectrometry. Chemical speciation results reveal bioavailable levels of Pb (4.05?µg/m3), Cr (10.75?µg/m3), Al (16.47?µg/m3), Cu (4.38E-01?µg/m3), and Ag (1.22E-02?µg/m3) in the airborne particulates. Pb and Cr levels exceeded the World Health Organization allowable limit of 0.5 and 2.5E-05?µg/m3, respectively. The labile phases showed strong indication of the presence of Cr and Cu metal. Excess cancer risks exposure for adults, outdoor workers and children were higher than the acceptable risk target level of 1E-06. Non-carcinogenic health risk estimated using hazard quotients (HQs) and hazard indices (HIs) showed ingestion route within the safe level of HI <1 implying no adverse effect while inhalation route exceeded the safe level for all receptors. Enforcement of pollution control by authorized agencies, and screening of greenbelts as sinks for air pollutants is strongly recommended. 相似文献
2.
AbstractVolatile organic compounds (VOCs) are some of the most important pollutants that have adverse effects on human health. In this study, simultaneous indoor and outdoor air samples were collected from four types of microenvironments (restaurants, photocopy centers, dry cleaners, and auto paint shops) in Kocaeli, Turkey in both winter and summer. Twenty-four VOCs were detected using gas chromatography and a flame ionization detector. The aim of the study was to determine the VOC concentrations in various workplaces and investigate associated health risks. Results show that the highest mean total VOC concentrations are in auto paint shops (14,066 µg/m3 in winter, 3441 µg/m3 in summer) followed by dry cleaners, restaurants, and photocopy centers. Total cancer risk was approximately 310 times greater than the acceptable limit in auto paint shops, 44 times greater in restaurants, 42 times greater in dry cleaners, and 17 times greater in photocopy centers. Total hazard quotients were also greater than the acceptable limit in all microenvironments. 相似文献
3.
AERMOD results were compared with the reported CALPUFF results to estimate the concentrations and temporal distributions of SO2 and NO2 from Tema Oil Refinery with particlar attention to heavy rainy season (HRS), minor rainy season (MRS), and dry season (DS). Statistical indices, including the fractional bias (FB), geometric mean variance (VG), normalized mean square error (NMSE), index of agreement (IOA), and geometric mean bias (MG), were used to assess the reliability of the models. Overall, AERMOD better predicted ambient SO2 and NO2 levels than the reported CALPUFF model. For SO2, AERMOD showed a good agreement with FB, IOA, and MG while CALPUFF showed a good prediction in NMSE and VG. Also, AERMOD predicted NO2 well with NMSE, IOA, MG, and VG compared with FB for CALPUFF. The MRS results showed higher hourly maximum concentrations (107.4 µg/m3 for SO2 and 31.7 µg/m3 for NO2). Maximum daily concentrations were slightly higher in HRS (37.7 µg/m3 for SO2 and 9.6 µg/m3 for NO2) compared to MRS and DS. The performace of the models may provide a better understanding for future epidemiological studies. 相似文献
4.
AbstractHuman health has been identified to be affected more significantly by indoor air quality. Among numerous pollutants present in indoor air, formaldehyde (FA) is of great concern because of its highly hazardous nature. The concentrations of FA were determined from 20 newly decorated homes in the city of Gonabad, Iran during 2015. It was found that the indoor air levels of FA in all the sampled houses were exceptionally high in the range of 21 to 360 µg/m3 (mean of 149.3 µg/m3). If the 24-h average concentrations of FA measured from those sites were concerned, nearly 40% of them were seen to exceed the WHO guideline values (i.e., 100 µg/m3). One of the important reasons for the high concentrations could be low air exchange rates in those houses (e.g., from 0.18 to 0.37?h?1), high levels of humidity in the newly decorating houses and stronger sources in the indoor environment. Furthermore, its pollution in homes with natural ventilation was seen to be much higher than those of mechanical ventilation. Due to high levels of indoor FA, more effective control procedures should be developed and employed to reduce the risk associated with formaldehyde exposure. 相似文献
5.
P. Rosenkranz N. Brüggemann H. Papen Z. Xu L. Horváth K. Butterbach-Bahl 《Plant and Soil》2006,286(1-2):301-322
During two intensive field campaigns in summer and autumn 2004 nitrogen (N2O, NO/NO2) and carbon (CO2, CH4) trace gas exchange between soil and the atmosphere was measured in a sessile oak (Quercus petraea (Matt.) Liebl.) forest in Hungary. The climate can be described as continental temperate. Fluxes were measured with a fully automatic measuring system allowing for high temporal resolution. Mean N2O emission rates were 1.5 μg N m−2 h−1 in summer and 3.4 μg N m−2 h−1 in autumn, respectively. Also mean NO emission rates were higher in autumn (8.4 μg N m−2 h−1) as compared to summer (6.0 μg N m−2 h−1). However, as NO2 deposition rates continuously exceeded NO emission rates (−9.7 μg N m−2 h−1 in summer and −18.3 μg N m−2 h−1 in autumn), the forest soil always acted as a net NO
x
sink. The mean value of CO2 fluxes showed only little seasonal differences between summer (81.1 mg C m−2 h−1) and autumn (74.2 mg C m−2 h−1) measurements, likewise CH4uptake (summer: −52.6 μg C m−2 h−1; autumn: −56.5 μg C m−2 h−1). In addition, the microbial soil processes net/gross N mineralization, net/gross nitrification and heterotrophic soil respiration as well as inorganic soil nitrogen concentrations and N2O/CH4 soil air concentrations in different soil depths were determined. The respiratory quotient (ΔCO2 resp ΔO2 resp−1) for the uppermost mineral soil, which is needed for the calculation of gross nitrification via the Barometric Process Separation (BaPS) technique, was 0.8978 ± 0.008. The mean value of gross nitrification rates showed only little seasonal differences between summer (0.99 μg N kg−1 SDW d−1) and autumn measurements (0.89 μg N kg−1 SDW d−1). Gross rates of N mineralization were highest in the organic layer (20.1–137.9 μg N kg−1 SDW d−1) and significantly lower in the uppermost mineral layer (1.3–2.9 μg N kg−1 SDW d−1). Only for the organic layer seasonality in gross N mineralization rates could be demonstrated, with highest mean values in autumn, most likely caused by fresh litter decomposition. Gross mineralization rates of the organic layer were positively correlated with N2O emissions and negatively correlated with CH4 uptake, whereas soil CO2 emissions were positively correlated with heterotrophic respiration in the uppermost mineral soil layer. The most important abiotic factor influencing C and N trace gas fluxes was soil moisture, while the influence of soil temperature on trace gas exchange rates was high only in autumn. 相似文献
6.
The characterization of indoor (a naturally ventilated office) and outdoor (adjacent courtyard) metals in PM2.5 during a winter period in Xi'an, China were carried out. The results indicated that the average mass concentrations of PM2.5 in indoor and outdoor environments all exceeded the daily average limit of 75 µg m–3 set by the Chinese government. The dominant metals in PM2.5 were Ca, Al, Zn, Mg, Fe, and Pb in both indoor and outdoor air. Concentration of As was much higher than the standard of 6 ng m–3 issued by the government. Enrichment factor analysis showed that anthropogenic emissions might be the primary sources of As, Cd, Pb, and Zn, while crust was the main origin of Co. A majority of indoor-to-outdoor concentration ratios of metal were lower than 1 indicating mostly the contribution of outdoor sources rather than indoor ones. As and Cr in both indoor and outdoor air posed the highest noncarcinogenic and carcinogenic risks, respectively. The noncarcinogenic and carcinogenic risks were 2.74 and 2.54 × 10?4 indoor and 4.04 and 3.87 × 10?4 outdoor, which suggested that possible adverse health effects should be of concern. 相似文献
7.
A study on the bacterioplankton of Conceição Lagoon (27°34′ S–48°27′ W), Southern Brazil, was carried out in July 2005 (austral winter) and January 2006 (austral summer) to characterize the bacterial spatiotemporal distribution and to determine the heterotrophic and photoautotrophic bacterial dominance in hypoxic/oxic stratified waters. Bacterial abundance increased significantly (p?0.05) in summer with averages of coccus cyanobacteria (CCY) ranging from 1.02?×?105 (winter) to 3.21?×?106 cells mL?1 (summer), heterotrophic coccus/rod-shaped (HCR) cells from 7.00?×?104 to 3.60?×?106 cells mL?1, and heterotrophic filamentous (HF) bacteria from 2.90?×?103 to 2.74?×?105 cells mL?1. Bacterial biovolumes also increased in summer with mean biovolumes of CCY ranging from 0.38 to 1.37 μm3, HCR cells from 0.31 to 1.12 μm3, and HF from 3.32 to 11.34 μm3. Principal component analysis showed that salinity, temperature, and light were the abiotic factors that better explained the temporal variability of bacterial assemblages. Bacterial heterotrophy dominated in the lagoon, excepted by the southern and part of central sector in January 2006, when autotrophic-dominated microbial community occurred. Spatially, bacterial assemblages were influenced by nutrient gradient, oxygen, and salinity with a positive relationship between biovolumes and nutrients and a negative relationship between abundance of coccus cyanobacteria and nutrients. area revealed a singular temporal pattern with hypoxic bottom waters in winter and oxygen-rich waters appearing in summer related with the availability of light and predominant microbes. Thus, oxygen consumption/production is likely to be regulated by the amount of light reaching the bottom, stimulating the production of oxygen by oxygenic phototrophs. 相似文献
8.
Ferdos Kord Mostafapour Jalil Jaafari Hamed Gharibi Mohammad Reza Sepand Mohammad Hoseini Davoud Balarak 《人类与生态风险评估》2018,24(4):1016-1029
A campaign was conducted to assess the PM1 concentration and elemental composition on the platforms and adjacent outdoor areas of an underground subway station (Imam Khomeini) and a surface subway station (Sadeghiye) in Tehran from June 2014 to November 2014. The respective mean concentrations of PM1 on the platforms and in the outdoor areas of Imam Khomeini station were 42.04 and 30.92 µg/m3 and for Sadeghiye station 31.42 and 26.02 µg/m3. Statistical analyses demonstrated that the platforms of the Imam Khomeini and Sadeghiye stations were influenced by the adjacent ambient air of these stations (p < 0.05). PM1 was found to be highly enriched with Fe on the platforms of metro systems, which were more frequently encountered in the Imam Khomeini station than the Sadeghiye station as 41.06% and 37.73% of the total PM1 mass respectively. Minor elements, particularly Ba, Pb, Cr, Cu, Ni, Mn, Ti, V, and Zn, were elevated for the platform of Imam Khomeini and, to a lesser degree, the platform of Sadeghiye stations, which may be due to abrasion processes between rail tracks, wheels, and brake pads. 相似文献
9.
Measurement of net ecosystem exchange,productivity and respiration in three spruce forests in Sweden shows unexpectedly large soil carbon losses 总被引:3,自引:1,他引:2
Anders Lindroth Leif Klemedtsson Achim Grelle Per Weslien Ola Langvall 《Biogeochemistry》2008,89(1):43-60
Measurement of net ecosystem exchange was made using the eddy covariance method above three forests along a north-south climatic gradient in Sweden: Flakaliden in the north, Knottåsen in central and Asa in south Sweden. Data were obtained for 2 years at Flakaliden and Knottåsen and for one year at Asa. The net fluxes (Nep) were separated into their main components, total ecosystem respiration (Rt) and gross primary productivity (Pg). The maximum half-hourly net uptake during the heart of the growing season was highest in the southernmost site with ?0.787 mg CO2 m?2 s?1 followed by Knottåsen with ?0.631 mg CO2 m?2 s?1 and Flakaliden with ?0.429 mg CO2 m?2 s?1. The maximum respiration rates during the summer were highest in Knottåsen with 0.245 mg CO2 m?2 s?1 while it was similar at the two other sites with 0.183 mg CO2 m?2 s?1. The annual Nep ranged between uptake of ?304 g C m?2 year?1 (Asa) and emission of 84 g C m?2 year?1 (Knottåsen). The annual Rt and Pg ranged between 793 to 1253 g C m?2 year?1 and ?875 to ?1317 g C m?2 year?1, respectively. Biomass increment measurements in the footprint area of the towers in combination with the measured net ecosystem productivity were used to estimate the changes in soil carbon and it was found that the soils were losing on average 96–125 g C m?2 year?1. The most plausible explanation for these losses was that the studied years were much warmer than normal causing larger respiratory losses. The comparison of net primary productivity and Pg showed that ca 60% of Pg was utilized for autotrophic respiration. 相似文献
10.
Currently, there is a lack of knowledge about GHG emissions, specifically N2O and CH4, in subtropical coastal freshwater wetland and mangroves in the southern hemisphere. In this study, we quantified the gas fluxes and substrate availability in a subtropical coastal wetland off the coast of southeast Queensland, Australia over a complete wet-dry seasonal cycle. Sites were selected along a salinity gradient ranging from marine (34 psu) in a mangrove forest to freshwater (0.05 psu) wetland, encompassing the range of tidal influence. Fluxes were quantified for CH4 (range ?0.4–483 mg C–CH4 h?1 m?2) and N2O (?5.5–126.4 μg N–N2O h?1 m?2), with the system acting as an overall source for CH4 and N2O (mean N2O and CH4 fluxes: 52.8 μg N–N2O h?1 m?2 and 48.7 mg C–CH4 h?1 m?2, respectively). Significantly higher N2O fluxes were measured during the summer months (summer mean 64.2 ± 22.2 μg N–N2O h?1 m?2; winter mean 33.1 ± 24.4 µg N–N2O h–1 m?2) but not CH4 fluxes (summer mean 30.2 ± 81.1 mg C–CH4 h?1 m?2; winter mean 37.4 ± 79.6 mg C–CH4 h?1 m?2). The changes with season are primarily driven by temperature and precipitation controls on the dissolved inorganic nitrogen (DIN) concentration. A significant spatial pattern was observed based on location within the study site, with highest fluxes observed in the freshwater tidal wetland and decreasing through the mangrove forest. The dissolved organic carbon (DOC) varied throughout the landscape and was correlated with higher CH4 fluxes, but this was a nonlinear trend. DIN availability was dominated by N–NH4 and correlated to changes in N2O fluxes throughout the landscape. Overall, we did not observe linear relationships between CH4 and N2O fluxes and salinity, oxygen or substrate availability along the fresh-marine continuum, suggesting that this ecosystem is a mosaic of processes and responses to environmental changes. 相似文献
11.
Dominika Kanikowska Maki Sato Satoshi Iwase Yuuki Shimizu Naoki Nishimura Yoko Inukai Junichi Sugenoya 《International journal of biometeorology》2013,57(3):475-481
The effects of environmental temperature on blood pressure and hormones in obese subjects in Japan were compared in two seasons: summer vs winter. Five obese (BMI, 32?±?5 kg/m2) and five non-obese (BMI, 23 ±3 kg/m2) men participated in this experiment at latitude 35°10′ N and longitude 136°57.9′ E. The average environmental temperature was 29?±?1 °C in summer and 3?±?1 °C in winter. Blood samples were analyzed for leptin, ghrelin, catecholamines, thyroid stimulating hormone (TSH), free thyroxine (fT4), free triiodothyronine (fT3), total cholesterol, triglycerides, insulin and glucose. Blood pressure was measured over the course of 24 h in summer and winter. A Japanese version of the Profile of Mood States (POMS) questionnaire was also administered each season. Systolic and diastolic blood pressures in obese men were significantly higher in winter (lower environmental temperatures) than in summer (higher environmental temperatures). Noradrenaline and dopamine concentrations were also significantly higher at lower environmental temperatures in obese subjects, but ghrelin, TSH, fT3, fT4, insulin and glucose were not significantly different in summer and winter between obese and non-obese subjects. Leptin, total cholesterol and triglyceride concentrations were significantly higher in winter in obese than non-obese men. Results from the POMS questionnaire showed a significant rise in Confusion at lower environmental temperatures (winter) in obese subjects. In this pilot study, increased blood pressure may have been due to increased secretion of noradrenaline in obese men in winter, and the results suggest that blood pressure control in obese men is particularly important in winter. 相似文献
12.
The emission of the airborne bacteria and fungi from an indoor wastewater treatment station adopting an integrated oxidation ditch with a vertical circle was investigated. Microbial samples were collected by the six-stage viable Andersen cascade impactor, and the samples were collected in triplicate in each sampling site per season. Culture-based method was applied to determine the concentrations of the airborne bacteria and fungi, while the cloning/sequencing method was used to characterize the genetic structure and community diversity of airborne bacteria. The highest concentrations of airborne bacteria (4155 ± 550 CFU/m3) and fungi (883 ± 150 CFU/m3) were obtained in June (summer). The lowest concentration of bacteria (1458 ± 434 CFU/m3) was determined in January (winter), and the lowest concentration of fungi (169 ± 40 CFU/m3) was found in March (spring), respectively. The particle size distribution analysis showed that most culturable bacteria obtained in all the sampling sites were in the particle size range of 1.1–4.7 µm. Most culturable fungi had particle sizes in the range 1.1–3.3 µm. Microbial population analysis showed that Bacillus sp., Acinetobacter sp., and Lysinibacillus were the main groups obtained in S1. Enterobacter was the dominant group in sampling site S2. Both the concentrations and particle size distribution of the bioaerosols in the enclosed space presented a seasonal and site-related variation. Concentration and richness of microorganisms in bioaerosols in June were higher than in September and January. The particle size distribution varied between the sampling sites, and proportion of large particles was higher in S2 than in S1 because of the settlement of large particles. Pathogenic species, such as Acinetobacter lwoffii, Staphylococcus saprophyticus, and Enterobacter sp., were isolated from the bioaerosols, which could pose serious latent danger to sewage workers’ health. 相似文献
13.
Anuradha Hora 《Preparative biochemistry & biotechnology》2013,43(8):769-784
Hexavalent chromium contamination is a serious problem due to its high toxicity and carcinogenic effects on the biological systems. The enzymatic reduction of toxic Cr(VI) to the less toxic Cr(III) is an efficient technology for detoxification of Cr(VI)-contaminated industrial effluents. In this regard, a chromate reductase enzyme from a novel Ochrobactrum sp. strain Cr-B4, having the ability to detoxify Cr(VI) contaminated sites, has been partially purified and characterized. The molecular mass of this chromate reductase was found to be 31.53 kD, with a specific activity 14.26 U/mg without any addition of electron donors. The temperature and pH optima for chromate reductase activity were 40°C and 8.0, respectively. The activation energy (Ea) for the chromate reductase was found to be 34.7 kJ/mol up to 40°C and the activation energy for its deactivation (Ed) was found to be 79.6 kJ/mol over a temperature range of 50–80°C. The frequency factor for activation of chromate reductase was found to be 566.79 s?1, and for deactivation of chromate reductase it was found to be 265.66 × 103 s?1. The reductase activity of this enzyme was affected by the presence of various heavy metals and complexing agents, some of which (ethylenediamine tetraacetic acid [EDTA], mercaptoethanol, NaN3, Pb2+, Ni2+, Zn2+, and Cd2+) inhibited the enzyme activity, while metals like Cu2+ and Fe3+ significantly enhanced the reductase activity. The enzyme followed Michaelis–Menten kinetics with Km of 104.29 µM and a Vmax of 4.64 µM/min/mg. 相似文献
14.
J. Brahney A. P. Ballantyne P. Kociolek S. Spaulding M. Otu T. Porwoll J. C. Neff 《Biogeochemistry》2014,120(1-3):259-278
Alpine lakes receive a large fraction of their nutrients from atmospheric sources and are consequently sensitive to variations in both the amount and chemistry of atmospheric deposition. In this study we explored the spatial changes in lake water chemistry and biology along a gradient of dust deposition in the Wind River Range, Wyoming. Regional differences were explored using the variation in bulk deposition, lake water, sediment, and bedrock geochemistry and catchment characteristics. Dust deposition rates in the Southwestern region averaged 3.34 g m?2 year?1, approximately three times higher than deposition rates in the Northwestern region (average 1.06 g m?2 year?1). Dust-P deposition rates ranged from 87 µg P m2 day?1 in the Northwestern region to 276 µg P m2 day?1 in the Southwestern region. Subalpine and alpine lakes in the Southwestern region had greater total phosphorus (TP) concentrations (5–13 µg L?1) and greater sediment phosphorus (SP) concentrations (2–5 mg g?1) than similar lakes elsewhere in the region (1–8 µg L?1 TP, 0.5–2 mg g?1 SP). Lake phosphorus concentrations were related to dissolved organic carbon (DOC) across vegetation gradients, but related to the percent of bare rock, catchment area to lake area, and catchment steepness across dust deposition gradients. Modern phytoplankton and zooplankton biomasses were two orders of magnitude greater in the Southwest than in the Northwest, and alpine lakes in the Southwest had a unique diatom species assemblage with relatively higher concentrations of Asterionella formosa, Pseudostaurosira pseudoconstruens, and Pseudostaurosira brevistriata. These results suggests that catchment controls on P export to lakes (i.e. DOC) are overridden in dominantly bare rock basins where poor soils cannot effectively retain dust deposited P. 相似文献
15.
Saisattha Noomnual 《人类与生态风险评估》2017,23(2):340-349
Traffic-related air pollution (TRAP), including particulate matter (PM) in respirable coarse and fine size fractions (PM10 and PM2.5), is known to have exposure effects on human health and environment. Real-time PM10 and PM2.5 concentrations were collected from the study locations in Bangkok, Thailand, using TSI AM510 particle counters. Temperature and % relative humidity (%RH) were also collected. Data were compared to data from the closest station of the Pollution Control Department (PCD), Thailand. Real-time mean concentration varied from 86 to 1107 µg/m3 (PM10) and varied from 25 to 664 µg/m3 (PM2.5). In addition, real-time mean PM10 (223.1 µg/m3) was nearly four times greater than that measured by the PCD station, 60 µg/m3. Temperature and %RH from real-time air monitoring and PCD station were comparable. In each study location (five locations, two in morning and afternoon/evening), there were significant positive correlations between PM10 and PM2.5 concentrations and significant negative correlations between temperature and RH%. Results suggested that outdoor TRAP via measured real-time PM concentrations were more realistic exposure concentration estimates among street vendors as related to respiratory and other symptoms than data obtained from PCD station. Nevertheless, PM10 as measured by the PCD station might be a reasonable surrogate for estimated outdoor PM2.5 exposure. 相似文献
16.
Rama Pal Ravinder Kaur Deepika Rajwar Jai Prakash Narayan Rai 《International journal of phytoremediation》2019,21(8):790-798
AbstractImpact of root Cd concentration on production of cysteine, non-protein thiols (NP-SH), glutathione (GSH), reduced glutathione (GSSG), and phytochelatins (PCs) in Eichhornia crassipes exposed to different dilutions of brass and electroplating industry effluent (25%, 50%, and 75%), and synthetic metal solutions of Cd alone (1, 2.5, and 3.5?ppm) and with Cr (1?ppm Cd + 1?ppm Cr, 2.5?ppm Cd + 3?ppm Cr, and 3.5?ppm Cd + 4?ppm Cr) was assessed in a 45?days study. Different treatments were used to understand and compare differential antioxidant defense response of plant under practical drainage (effluent) and experimental synthetic solutions. The production of NP-SH and cysteine was maximum under 2.5?ppm Cd + 3?ppm Cr treatments i.e., 1.78?µmol/g fw and 288?nmol/g fw, respectively. The content of GSH declined whereas that of GSSG increased progressively with exposure duration in all treatments. HPLC chromatograms revealed that the concentrations of PC2, PC3, and PC4 (248, 250, and 288?nmol-SH equiv.g?1 fw, respectively) were maximum under 1?ppm Cd, 1?ppm Cd + 1?ppm Cr, and 2.5?ppm Cd + 3?ppm Cr treatments, respectively. PC2, PC3, and PC4 concentrations increased with Cd accumulation in the range 812–1354?µg/g dry wt, 1354–2032?µg/g dry wt and 2032–3200?µg/g dry wt, respectively. Thus, the study establishes a direct proportionality relationship between concentration/length of phytochelatins and root Cd concentrations, upto threshold limits, in E. crassipes. 相似文献
17.
The present study was aimed to determine the effect of GnRH analog (buserelin acetate) on the quality of bovine spermatozoa stored at 16°?C for 24?h. Semen collected in the summer season from June to September from healthy Polish Holstein–Friesian bulls. Ejaculates were centrifuged, divided and diluted to the final concentration of 240?×?106 spermatozoa/mL using animal protein–free commercial BIOXcell® extender (IMV Technologies, L’aigle, France) (Control) or with BIOXcell® extender supplemented with buserelin acetate and stored 0, 8 and 24?h. Sperm motility parameters analysis was performed using a computer-assisted sperm analysis (CASA) system. The viability of spermatozoa was performed using flow cytometer. The addition of buserelin acetate to BIOXcell® extender did positively affect the total motility (was higher in the observed samples with the addition of 2?µg/mL and 4?µg/mL than in the control group), progressive motile (forward progressing sperm was significantly increased (p?<?0.05) over the control group at the 0?h and 8?h of incubation following the supplementation of 2, 4 and 8?μg/mL buserelin acetate) and viability of spermatozoa (the number of live spermatozoa was significantly higher (p?<?0.05) in 2?µg/mL and 4?µg/mL samples with buserelin acetate at 8th hour of incubation and in sample with 4?µg/mL at 24th hour of incubation compared to the control group). We recommend adding 4?µg/mL to the extender to improve the quality of bovine semen. 相似文献
18.
Florian Wittmann Bianca Thaís Zorzi Fernando Augusto Tambelini Tizianel Marcus Vinicius Santiago Urquiza Rogério Rodrigues Faria Nathália Machado e Sousa Érica de Souza Módena Roberto Macedo Gamarra Augusto Lisboa Martins Rosa 《Folia Geobotanica》2008,43(4):397-411
The aboveground wood biomass (AWB) of tropical forests plays an important role in the global carbon cycle, and local AWB estimates provide essential data that enable the extrapolation of biomass stocks to ecosystem or biome-wide carbon cycle modelling. Few AWB estimates exist in Neotropical freshwater floodplains, where tree species distribution and forest structure depend on the height and duration of periodic inundations. We investigated tree species composition, forest structure, wood specific gravity, and AWB of trees ≥10 cm dbh in 16 plots totalling an area of 1 ha in a seasonally inundated riparian forest of the lower Miranda River, southern Pantanal, Brazil. The 443 tree individuals belonged to 46 species. Four species (Inga vera, Ocotea suaveolens, Tabebuia heptaphylla and Cecropia pachystachya) comprised more than 50% of the Total Importance Values (TIV), and floristic similarities between the plots averaged 38%. Although we detected an overall increase in species diversity correlated with decreasing flood levels, the most important tree species had almost identical distribution patterns along the flooding gradient. The stand basal area per plot (±?s.d.) amounted to 3.0?±?1.1 m2 (47.8?±?18.1 m2/ha), and the tree heights averaged 10.9?±?1.4 m. Multiplying the individual basal areas by individual tree heights and a form factor of 0.6, we estimated the aboveground wood volume (AWV) for each individual, and for each plot (24.4?±?11.7 m3, 391.1?±?188 m3/ha). Wood specific gravity (SG) varied between 0.39 g/cm3 (Cecropia pachystachya) and 0.87 g/cm3 (Tabebuia heptaphylla), with a stand level average of 0.63?±?0.12 g/cm3. Multiplying the individual AWV with species SG, we estimated the plot AWB to be 16.2?±?6.4 Mg (259.4?±?102 Mg/ha). This value is comparable to that reported for late-successional forest stands of Amazonian floodplain forests, and it is close to the worldwide tropical average AWB. Because tree heights in the present forest were comparatively low when compared to other Neotropical forests, we found that resprouting of stems accounted for comparatively high basal areas. We argue that stem resprouting is an adaptation of tree species originating in non-flooded Cerrado to the seasonal inundations of riparian forests. 相似文献
19.
Anna Kozajda Karolina Jeżak Marcin Cyprowski Irena Szadkowska-Stańczyk 《Aerobiologia》2017,33(4):481-491
The aim of the study was to assess the levels of inhalable dust, endotoxins and (1–3)-β-d-glucans as agents harmful to the respiratory tract of workers of municipal waste sorting plants and interaction between these agents based on the measurements taken in two plants with different processing capacities. The study was conducted in summer season in two waste sorting plants (WSPs) differing in processing capacity. Samples of bioaerosol for inhalable dust (gravimetric method), endotoxins (LAL test in kinetic, chromogenic version) and (1–3)-β-d-glucans (Glucatell test in kinetic version) were collected from 42 sorting workers using individual aspirators with glass fiber filters during the work shift. Average geometric concentrations (geometric standard deviation; min–max) of inhalable dust, endotoxins and (1–3)-β-d-glucans were: WSP1: 1.7 mg m?3 (2.2; 0.6–6.9 mg m?3); 15.9 ng m?3 (2.1; 5.4–78.9 ng m?3), 55.1 ng m?3 (1.8; 20.7–188.6 ng m?3) and WSP2: 0.8 mg m?3 (2.2; 0.2–3.8 mg m?3), 9.8 ng m?3 (2.4; 1.6–29.7 ng m?3), 45.0 ng m?3 (3.2, 5.7–212.9 ng m?3), respectively. A significantly higher concentration of inhalable dust was recorded in WSP1 with bigger processing capacity compared to WSP2 (less processing capacity). Significant (p < 0.05) and very high correlations (Spearman rank R > 0.7) were found between the concentrations of all analyzed harmful agents. Processing capacity of waste sorting plants differentially affects the concentrations of inhalable dust, whereas concentrations of endotoxins and glucans are less clearly affected. This suggests that relative concentrations of endotoxin and glucan are depending on the waste sorting capacity. 相似文献
20.
Minaleshewa Atlabachew Bhagwan Singh Chandravanshi Mesfin Redi 《Biological trace element research》2010,138(1-3):316-325
The levels of essential (Ca, Mg, Mn, Fe, Zn, Cr, Cu, and Co) and non-essential (Cd and Pb) metal in six different varieties of Ethiopian khat (Catha edulis Forsk, an evergreen stimulant plant) commonly consumed in the country and exported to the neighboring countries were determined by flame atomic absorption spectrometry. Known weight of oven-dried khat samples were wet-digested using 2 mL of (69–72%) HNO3 and 2 mL of (70%) HClO4 for 2 h and 30 min at variable temperature (120–270°C). The mineral contents in the digests were analyzed using flame atomic absorption spectrometer. The following concentrations ranges in fresh-weight basis were recorded in decreasing order: Ca (1,038–2,173 µg/g)?>?Mg (478.2–812.3 µg/g)?>?Fe (53.95–82.83 µg/g)?>?Zn (5.18–9.40 µg/g)?>?Mn (6.98–8.66 µg/g)?>?Cu (1.85–5.53 µg/g)?>?Cr (0.66–3.47 µg/g)?>?Co (0.41–0.80 µg/g). A wide variation in the mineral contents of khat from different region of Ethiopia was noticed. The toxic metals (Pb and Cd) were not detected in all the samples analyzed. 相似文献