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1.
Michael R. Zimmerman 《American journal of physical anthropology》1979,51(2):235-253
The difficulties of diagnosis of pathologic conditions are immensely magnified when the subject of a postmortem examination has been postmortem for several hundreds to thousands of years. Artefacts of decomposition and bacterial and fungal invasion are compounded upon those of rehydration when mummified tissue is examined. As an approach to these problems, a study of the changes seen in experimentally mummified and rehydrated tissues was undertaken. Normal and pathologic tissues were studied in comparison to sections prepared from the fresh tissue. The experimentally mummified tissues were generally similar to, but somewhat better preserved than, actual human mummies. There was organ and tissue specific variability in preservation, and different classes of pathology likewise showed differential preservation. Inflammatory reactions were not very well-preserved although infecting microorganisms were easily identified. Degenerative processes such as atherosclerosis and others characterized by the accumulation of abnormal products, were well preserved, while necrosis, as in acute myocardial infarction, was not. Malignancies were particularly well preserved. The implications of these findings for previous and future mummy studies is discussed in terms of our understanding of the evolution of disease processes. 相似文献
2.
The present findings show that both elastic system fibers and collagen markedly resisted change in tissues more than 2000 years old. The distribution of elastic fibers and elastic-related fibers (namely, oxytalan and elaunin fibers) in mummified tissues coincided with the observations made on the modern human tissues used as controls. The collagenous structures present in tissue sections obtained from the Egyptian mummy studied took on a deeply red colour when stained in the Picrosirius solution indicating that, as well as in the fresh controls, the basic groups in the collagen molecules were available for reacting with the strongly acidic dye Sirius Red. When viewed with polarized light, the collagen in the same tissue sections displayed an increased birefringence, which shows that the collagen molecules in mummified tissues maintain the oriented disposition which is typical of the modern human tissues used as controls. The methods employed have proved to be useful for the delineation of the elastic system fibers and of the collagenous scaffolding, which may be used as valuable landmarks in the study of the histoarchitecture of organs that have undergone considerable distortion. 相似文献
3.
Michael R. Zimmerman 《American journal of physical anthropology》1972,37(2):271-280
Tissue mummified by desiccation can be examined histologically after rehydration with Ruffer's solution, and a number of natural mummies have been studied by this technique. In order to study the efficacy of the rehydration process, and to evaluate the degree of artefact introduced, normal human tissues were desiccated, rehydrated, and examined histologically. There was generally good preservation of architecture and moderate preservation of cellular detail, with some organ specific variability. The prospect of an atlas of mummified pathological lesions is discussed, as an aid to the determination of disease patterns in prehistoric populations. 相似文献
4.
H Piepenbrink J Frahm A Haase D Matthaei 《American journal of physical anthropology》1986,70(1):27-28
Clinical applications of nuclear magnetic resonance (NMR) imaging have demonstrated its great potential for noninvasive examinations of soft tissues. Here preliminary experiments are reported on mummified human tissue by means of NMR cross-sectional and transmission imaging. For this purpose the natural viscosity of the tissues has been lowered by rehydration with an aqueous solution of 20% acetone. 相似文献
5.
Survival of Monilinia fructicola on the surface of mummified fruit and in peduncles and shoots of stone fruit trees infected by M. fructicola in the previous season was studied from 2003 to 2006 in orchards in the agricultural region of Beijing. Viable conidia of M. fructicola were consistently detected on fruit mummies from mid‐March to the end of April. During flowering (in mid‐April), studies in five peach orchards showed that 33–87% of mummified fruit bore viable conidia. The germination rate of conidia on diseased fruit was about 64% in autumn. It decreased to 24% in mid‐winter when the fruit was completely mummified, and in the following year to 2–4% in early spring. Viable M. fructicola was consistently detected in peach and nectarine shoots collected in winter and spring. In general, viable M. fructicola in peduncles was detected from mummified fruit of 11–27% branches and from asymptomatic plant tissues of 3–20% branches. Sporulation of M. fructicola was observed on peduncles in seven of eight surveys, and the percentage of branches containing viable M. fructicola in peduncles in contact with mummified fruits ranged from 18% to 40%. This study demonstrates that the tree‐borne mummified fruit and the peduncles could be the parts of trees where M. fructicola can survive the winter in orchards in suburban Beijing. 相似文献
6.
R Boano E Fulcheri R Grilletto E Leospo E Rabino Massa 《Bollettino della Società italiana di biologia sperimentale》1999,75(7-8):39-45
The purpose of this work is to give a brief account of the possibility to estimate the preservation of human mummified tissues using histological analysis. This method can be useful to identify injuries and to plan qualified conservative actions on ancient human remains. Some preliminary results are presented here regarding the study on 16 ancient mummies from the Egyptian Museum of Turin. Samples of mummified tissues were taken without damaging the remains; they were hydrated again and dyed with histological techniques which were specifically modified and verified in same cases. Our research identifies some agents (biological and chemical) that appear to be responsible, among others, of mummified tissue destruction. The microscopic examination reveals features that might refer to fungal and bacterial infestation. Using special staining methods on seried sections we were able to suspect, at least in one case, the presence of biologically active forms. Microbiological assays confirm the vitality of fungi. Histological tissue analysis can then be useful to guide any conservative intervention for preservation and protection of the integrity of biological remains from museum collections. 相似文献
7.
Summary The present findings show that both elastic system fibers and collagen markedly resisted change in tissues more than 2000 years old.The distribution of elastic fibers and elastic-related fibers (namely, oxytalan and elaunin fibers) in mummified tissues coincided with the observations made on the modern human tissues used as controls.The collagenous structures present in tissue sections obtained from the Egyptian mummy studied took on a deeply red colour when stained in the Picrosirius solution indicating that, as well as in the fresh controls, the basic groups in the collagen molecules were available for reacting with the strongly acidic dye Sirius Red. When viewed with polarized light, the collagen in the same tissue sections displayed an increased birefringence, which shows that the collagen molecules in mummified tissues maintain the oriented disposition which is typical of the modern human tissues used as controls.The methods employed have proved to be useful for the delineation of the elastic system fibers and of the collagenous scaffolding, which may be used as valuable landmarks in the study of the histoarchitecture of organs that have undergone considerable distortion.Supported in part by Grant no. 43.83.0610/00 from Financiadora de Estudos e Projetos (FINEP-FNDCT). G.S. Montes is Career Investigator of the Brazilian National Research Council (CNPq) 相似文献
8.
A method of reconstituting mummified tissues using the fabric softener “Comfort” is described. Routine histological paraffin wax sections were prepared following overnight treatment at room temperature with a 0.2% solution of this substance in isotonic saline. A comparison of results obtained by this method is made with those obtained using Ruffer's and Sandison's solutions. Nuclear stains gave poor results whereas connective tissue stains demonstrated their respective fibers by all rehydration methods. 相似文献
9.
Ivan Mikšík Pavla Sedláková Statis Pataridis Federica Bortolotti Rossella Gottardo 《Protein science : a publication of the Protein Society》2016,25(11):2037-2044
Proteins and their modifications of the natural mummy of Cangrande della Scala (Prince of Verona, Northern Italy, 1291–1329) were studied. The nano‐LC‐Q‐TOF analysis of samples of rib bone and muscle from the mummy showed the presence of different proteins including Types I, III, IV, V, and XI collagen, hemoglobin (subunits alpha and beta), ferritin, biglycan, vitronectin, prothrombin, and osteocalcin. The structure of Type I and Type III collagen was deeply studied to evaluate the occurrence of modifications in comparison with Type I and Type III collagen coming from tissues of recently died people. This analysis showed high percentage of asparaginyl and glutaminyl deamidation, carbamylation and carboxymethylation of lysine, as well as oxidation and dioxidation of methionine. The most common reaction during the natural mummification process was oxidation—the majority of lysine and proline of collagen Type I was hydroxylated whereas methionine was oxidated (oxidated or dioxidated). To the best of our knowledge, this is the first study which reports the protein profile of a natural mummified human tissue and the first one which describes the carbamylation and carboxymethylation of lysine in mummified tissues. 相似文献
10.
A method of reconstituting mummified tissues using the fabric softener "Comfort" is described. Routine histological paraffin wax sections were prepared following overnight treatment at room temperature with a 0.2% solution of this substance in isotonic saline. A comparison of results obtained by this method is made with those obtained using Ruffer's and Sandison's solutions. Nuclear stains gave poor results whereas connective tissue stains demonstrated their respective fibers by all rehydration methods. 相似文献
11.
The aim of this study was to review all Pubmed®-listed palaeopathological studies performed on pre-Columbian South American mummies. A total of 61 studies were found (1977–2005). Review criteria included e.g. method of examination, method of mummification, palaeopathological diagnoses and individual age of mummies as well as dating, which ranged from 7500 to 500 years BP, mainly (if reported) originating from the Chiribaya and Chinchorro cultures. The average age of the 99 individually reported mummies was about 25 years. Only six studies included computed tomography, thirteen studies used classical radiography as an examination method. Three studies analysed parasite related diseases, especially caused by Trypanosoma cruzi. Among all of the reported infectious diseases (n = 9), there were seven studies presenting cases of tuberculosis. The results were also compared inter-culturally. In 61 studies (37 epidemiological and 24 case reports) more than 6400 mummified individuals were analysed. By contrast, meta-analytic data for ancient Egyptian mummies ( Zweifel et al., 2009) included about 3000 analysed individuals in 131 studies (85 case reports and 46 epidemiological studies). In general, ancient Egyptian mummies were shown to be intentionally mummified, whereas the Pre-Columbian American mummies showed a great diversity of spontaneous mummification. However, ritualistic mummification methods were also practised (n = 2). This study's results shall assist to improve evidence-based research in palaeopathology. 相似文献
12.
Natallia Shved Cordula Haas Christina Papageorgopoulou Guelfirde Akguel Katja Paulsen Abigail Bouwman Christina Warinner Frank Rühli 《PloS one》2014,9(10)
Mummified human tissues are of great interest in forensics and biomolecular archaeology. The aim of this study was to analyse post mortem DNA alterations in soft tissues in order to improve our knowledge of the patterns of DNA degradation that occur during salt mummification. In this study, the lower limb of a female human donor was amputated within 24 h post mortem and mummified using a process designed to simulate the salt dehydration phase of natural or artificial mummification. Skin and skeletal muscle were sampled at multiple time points over a period of 322 days and subjected to genetic analysis. Patterns of genomic fragmentation, miscoding lesions, and overall DNA degradation in both nuclear and mitochondrial DNA was assessed by different methods: gel electrophoresis, multiplex comparative autosomal STR length amplification, cloning and sequence analysis, and PCR amplification of different fragment sizes using a damage sensitive recombinant polymerase. The study outcome reveals a very good level of DNA preservation in salt mummified tissues over the course of the experiment, with an overall slower rate of DNA fragmentation in skin compared to muscle. 相似文献
13.
Marin A Cerutti N Massa ER 《Bollettino della Società italiana di biologia sperimentale》1999,75(5-6):27-30
We conducted a molecular investigation of the presence of sicklemia in six predynastic Egyptian mummies (about 3200 BC) from the Anthropological and Ethnographic Museum of Turin. Previous studies of these remains showed the presence of severe anemia, while histological preparations of mummified tissues revealed hemolytic disorders. DNA was extracted from dental samples with a silica-gel method specific for ancient DNA. A modification of the polymerase chain reaction (PCR), called amplification refractory mutation system (ARMS) was then applied. ARMS is based on specific priming of the PCR and it permits diagnosis of single nucleotide mutations. In this method, amplification can occur only in the presence of the specific mutation being studied. The amplified DNA was analyzed by electrophoresis. In samples of three individuals, there was a band at the level of the HbS mutated fragment, indicating that they were affected by sicklemia. On the basis of our results, we discuss the possible uses of new molecular investigation systems in paleopathological diagnoses of genetic diseases and viral, bacterial and fungal infections. 相似文献
14.
Biologic rhythms give insight into normal physiology and disease. They can be used as biomarkers for neuronal degenerations. We present a diverse data set to show that hair and teeth contain an extended record of biologic rhythms, and that analysis of these tissues could yield signals of neurodegenerations. We examined hair from mummified humans from South America, extinct mammals and modern animals and people, both healthy and diseased, and teeth of hominins. We also monitored heart-rate variability, a measure of a biologic rhythm, in some living subjects and analyzed it using power spectra. The samples were examined to determine variations in stable isotope ratios along the length of the hair and across growth-lines of the enamel in teeth. We found recurring circa-annual periods of slow and fast rhythms in hydrogen isotope ratios in hair and carbon and oxygen isotope ratios in teeth. The power spectra contained slow and fast frequency power, matching, in terms of normalized frequency, the spectra of heart rate variability found in our living subjects. Analysis of the power spectra of hydrogen isotope ratios in hair from a patient with neurodegeneration revealed the same spectral features seen in the patient's heart-rate variability. Our study shows that spectral analysis of stable isotope ratios in readily available tissues such as hair could become a powerful diagnostic tool when effective treatments and neuroprotective drugs for neurodegenerative diseases become available. It also suggests that similar analyses of archaeological specimens could give insight into the physiology of ancient people and animals. 相似文献
15.
There has been much debate regarding the impact of parasitoid competition and hyperparasitism on the successful biological control of aphid pests. Difficulty in the evaluation of interspecific interactions and trophic links using conventional rearing and dissection methods has prevented a deeper understanding of such relationships. The analysis of trophic links in the parasitoid community associated with the melon aphid (Aphis gossypii) in Hawaii provides a unique opportunity to assess complex interactions that occur in a system where all of the aphids and parasitoids have been introduced. Here, we developed and applied multiplex PCR assays to investigate the occurrence of in-host competition between parasitoids and/or hyperparasitoids on melon aphids collected from fields of Colocasia esculenta. To fully document the parasitoid-hyperparasitoid community within A. gossypii, both live and mummified aphids were examined. A total of 818 live and 245 mummified aphids were analyzed using the multiplex assays, with congruent rearing of over 600 mummified aphids serving as a basis for qualitative comparisons in terms of species composition and trophic linkages. The rearing and the DNA methods showed similar trends, with sharp declines in one parasitoid species followed by sharp increases in another during the course of the season. Molecular analyses revealed that hyperparasitism and multiparasitism of live aphids is remarkably low, whereas hyperparasitism of mummified aphids was extraordinarily high in both rearing and molecular analyses. In comparison to reared samples, molecular analysis of the parasitoid community was more complete and permitted the identification of previously unknown or unconfirmed trophic linkages. The potential of this approach in future studies on the biological control of aphids in Hawaii, particularly in light of new parasitoid introductions, is discussed. 相似文献
16.
Nitzan Soffer Marilyn E Brandt Adrienne MS Correa Tyler B Smith Rebecca Vega Thurber 《The ISME journal》2014,8(2):271-283
White plague (WP)-like diseases of tropical corals are implicated in reef decline worldwide, although their etiological cause is generally unknown. Studies thus far have focused on bacterial or eukaryotic pathogens as the source of these diseases; no studies have examined the role of viruses. Using a combination of transmission electron microscopy (TEM) and 454 pyrosequencing, we compared 24 viral metagenomes generated from Montastraea annularis corals showing signs of WP-like disease and/or bleaching, control conspecific corals, and adjacent seawater. TEM was used for visual inspection of diseased coral tissue. No bacteria were visually identified within diseased coral tissues, but viral particles and sequence similarities to eukaryotic circular Rep-encoding single-stranded DNA viruses and their associated satellites (SCSDVs) were abundant in WP diseased tissues. In contrast, sequence similarities to SCSDVs were not found in any healthy coral tissues, suggesting SCSDVs might have a role in WP disease. Furthermore, Herpesviridae gene signatures dominated healthy tissues, corroborating reports that herpes-like viruses infect all corals. Nucleocytoplasmic large DNA virus (NCLDV) sequences, similar to those recently identified in cultures of Symbiodinium (the algal symbionts of corals), were most common in bleached corals. This finding further implicates that these NCLDV viruses may have a role in bleaching, as suggested in previous studies. This study determined that a specific group of viruses is associated with diseased Caribbean corals and highlights the potential for viral disease in regional coral reef decline. 相似文献
17.
Lena Öhrström Andreas Bitzer Markus Walther Frank Jakobus Rühli 《American journal of physical anthropology》2010,142(3):497-500
Ancient mummified soft‐tissues are a unique source to study the evolution of disease. Diagnostic imaging of such historic tissues is of foremost interest in paleoanthropology or paleopathology, with conventional x‐ray and computed tomography (CT) being the gold‐standard. Longer wavelength radiation in the far‐infrared or Terahertz region allows diagnostic close‐to‐surface tissue differentiation of bone morphology while being harmless to human cells. The aim of this study is to show the feasibility and the morpho‐diagnostic impact of THz imaging of historic remains. Images of an artificially embalmed ancient Egyptian human mummy hand, an artificially embalmed ancient Egyptian mummified fish and a macerated human lumbar vertebra were obtained by THz‐pulse imaging and compared with conventional X‐ray and CT images. Although conventional x‐ray imaging provides higher spatial resolution, we found that THz‐imaging is well‐suited for the investigation of ancient mummified soft tissue and embalming‐related substances / wrappings. In particular, bone and cartilaginous structures can be well differentiated from surrounding soft‐tissues and bandage‐wrappings by THz imaging. Furthermore, THz‐pulse imaging also measures the time‐delay of the pulsed signal when passing through the sample, which provides supplementary information on the optical density of the sample that is not obtained by X‐ray and CT. Terahertz radiation provides a completely non‐invasive diagnostic imaging modality for historic dry specimens. We anticipate this modality also to be used for detection of hidden objects in historic samples such as funerary amulets still in situ in wrapped mummies, as well as potentially for the identification of spectral signatures from chemical substances, e.g., in embalming essences.. Am J Phys Anthropol, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
18.
Sequence homology of a canine brain calcium-binding protein with calregulin and the human Ro/SS-A antigen 总被引:1,自引:0,他引:1
J H Collins Z J Xi B H Alderson-Lang S Treves P Volpe 《Biochemical and biophysical research communications》1989,164(1):575-579
A 60 kDa calcium-binding protein (CBP) was purified from canine brain and its N-terminal sequence determined to be: Glu-Pro-Ala-Ile-Tyr-Phe-Lys-Glu-Gln-Phe-Leu-Asp-Gly-Asp-Gly-X-Thr-Arg-X- Ile- Glu-Ser-Lys. This sequence is very similar to that of "Ccalregulin", a CBP of unknown function which is similar in size and appears to be present in most animal tissues. An unexpected and even more striking similarity was found with the N-terminal sequence of the human Ro/SS-A antigen, a 60 kDa protein which has long been implicated in the pathogenesis of autoimmune diseases such as systemic lupus erythematosus. These findings suggest that the Ro/SS-A antigen is probably also a CBP. 相似文献
19.
The objective of this study was to investigate the timing of foetal mortality in gilts of a segregating F2 cross of Large White and Meishan pigs on the basis of the length distribution of mummified foetuses and the frequency of non-fresh stillborn piglets in order to establish whether critical periods for foetal mortality exist. All expelled conceptuses and placentae of 192 farrowing gilts with a normal health status were meticulously investigated to recover all mummified foetuses. The length of each mummified foetus was measured. The predicted number of foetuses present per gilt at the early foetal stage of gestation was calculated as the sum of numbers of mummified foetuses and non-fresh stillborn, fresh stillborn and liveborn piglets. Foetal loss was calculated as the sum of mummified foetuses and non-fresh stillborn piglets. The average foetal mortality rate per gilt was 8.7%. In total 162 mummified foetuses were found (average 0.84 per litter), ranging in length from 0.4 to 33.0 cm. This indicates a range in foetal age at death of approximately 35-100 days. Although mummified foetuses of all lengths within the above mentioned range were found, relatively many had a length of less than 4 cm or of 10-21 cm. The total number of non-fresh stillborn piglets (i.e. late foetal deaths) was 58 (average 0.30 per litter). It can be concluded that foetal mortality occurred in these gilts throughout the period from day 35 to term, with relatively high incidences at the early foetal stage (days 35-40), shortly after mid-pregnancy (days 55-75) and after approximately day 100 of gestation. These three periods coincide with reported periods of change in porcine placental growth. 相似文献
20.
中国木化石资源虽然很丰富, 但研究程度相比叶片化石要低得多, 且主要集中在中国北部和西南地区, 研究领域大多局限于分类学方面, 而木化石分析与古气候、古生态等领域的交叉和系统研究还很少。大多数情况下木化石的保存方式都为石化或矿化保存, 而木乃伊化保存的木化石则非常罕见。木乃伊化保存的化石在植物分类、系统演化及古气候研究等方面具有独特而重要的价值。近年来, 本团队在华南地区发现大量木乃伊化保存的新生代木化石, 它们分别产自广西南宁盆地晚渐新世邕宁组、广西桂平盆地中新世二子塘组以及广东茂名盆地晚更新世地层中。本文总结了这些木乃伊化保存的木化石的研究进展, 包括多样性组成、古植被、古气候及古生态等特征, 并对木兰属、蒲桃属和枫香属的植物地理史进行了讨论。综合分析植物群组成特征和木材生长轮以及木化石中具有气候指示意义的解剖结构特征, 推测广西南宁盆地晚渐新世时期生长着常绿(少数落叶)阔叶林, 为温暖湿润的亚热带季节性气候; 广西桂平盆地中新世时期生长着常绿—落叶、阔叶—针叶混交林 (阔叶为主), 为温暖湿润的亚热带气候; 广东茂名盆地晚更新世生长着常绿—落叶、阔叶—针叶混交林, 为温暖湿润的亚热带至热带季风气候。这是中国低纬度地区首次系统研究木乃伊化保存的木化石, 为新生代植物群的系统演化和古环境分析等提供了重要的木化石依据。 相似文献