首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 109 毫秒
1.
The goal of this study was to examine whether supplemental fructooligosaccharides (FOS) plus mannanoligosaccharides (MOS) influenced immune function and ileal and fecal microbial populations of adult dogs. Eight adult dogs surgically fitted with ileal cannulas were used in a crossover design. Dogs were fed 200g of a dry, extruded, kibble diet twice daily. At each feeding, dogs were dosed with either 1g sucrose (placebo) or 2g FOS plus 1g MOS orally via gelatin capsule. Fecal, ileal, and blood samples were collected at the end of each 14-d period to measure microbial populations and immune characteristics. Treatment least squares means were compared using the GLM procedure of SAS. Supplementation of FOS plus MOS increased fecal bifidobacteria and fecal and ileal lactobacilli concentrations. Dogs fed FOS plus MOS also tended to have lower blood neutrophils and greater blood lymphocytes vs placebo. Serum, fecal, and ileal immunoglobulin concentrations were unchanged by treatment. Supplementation of FOS plus MOS beneficially altered indices of gut health by improving ileal and fecal microbial ecology. Supplementation of FOS plus MOS also altered immune function by causing a shift in blood immune cells.  相似文献   

2.
"食品微生物学"是食品质量与安全专业的基础课,"食品微生物学检验"为后期专业课程,原教学设计为两门相对独立的课程。教学中发现两门课程在理论、实验教学内容上存在重复和交叉,不利于"教"与"学"的深度融合。为了打破两门课程间的壁垒,我们将理论课程内容进行融合与贯通,实现模块式对接;对实验课程进行整合与设计,将课程内容镶嵌为综合性实验。经过3年的教学实践,我们初步构建了跨课程整合-对接镶嵌式教学模式,不仅提高了教学效率,同时增强了学生专业能力培养和综合素质提高,也为其他课程教学改革提供了借鉴。  相似文献   

3.
A considerable increase in creatinine kinase (CK) activity and gastrin hormone due to exercise has been observed in sled dogs during endurance mushing races; however, there have been no studies on sled dogs during recreational mushing. Although oxytocin hormone is involved in social behaviors and empathy, it has not been studied in sled dogs. This study aimed to assess changes in plasma CK activity, and gastrin and oxytocin concentrations in adult sled dogs used in touristic mushing in North Patagonia, Argentina. Blood samples were collected before, during, and after the winter season of 2017. Creatinine kinase activity measurement was done using an enzymatic assay. Hormone analyses were performed using commercial Enzyme-Linked InmunoSorbent Assay kits. Results showed an expected two-fold increase in CK activity during the winter, with recovering basal values after winter (< 400 UI/L), low and stable levels of gastrin (9.4 ± 8.8 pg/mL), and a slight increase in oxytocin (23%) after mushing activities. No evidence indicated gastrin alterations or muscular damage from touristic mushing, but an oxytocin increase would indicate a stimulation of the brain reward system.  相似文献   

4.
目的寻找某实验比格犬繁育基地群发性泌尿系统结石的病因及其有效的防治措施。方法采用临床诊断、病理诊断、结石成分分析、饲料成分分析、回顾性调查分析等手段以及综合的处理措施。结果经分析发现该群发性泌尿系统结石是由肉粉引起的尿酸盐结石,通过碱化尿液、降低血尿酸和去除病因等方法后,全群得到了有效的治疗。结论该肉粉是引起此次群发性泌尿系统结石的主要原因,由尿酸盐引起的、未发生器质性病变的尿路结石症可以治愈。  相似文献   

5.
研究了捕自安徽宿州的成年雄性山地麻蜥 (Eremiasbrenchleyi)选择体温、热耐受性、温度对食物同化和运动表现的影响。结果显示 :①选择体温、临界高温和临界低温的平均值分别为 33 7、 43 6和 3 3℃。②环境温度在 2 6~ 38℃时 ,对山地麻蜥食物通过时间、摄食量、表观消化系数 (ADC)、同化效率 (AE)和运动表现有显著的影响 ;在 2 6~ 30℃时食物通过时间随温度升高而缩短 ,超过 30℃则随温度升高而延长 ;在 2 6和 2 8℃时 ,摄食量、ADC和AE均小于更高温度的对应值。③体温在 19~ 34℃ ,蜥蜴的疾跑速随体温上升而加快 ,31和 34℃时最快 ,超过 34℃后随体温升高而减慢 ;31和 34℃时的持续运动距离最长 ,超过 36℃后随体温升高而显著缩短 ,但体温 19~ 36℃蜥蜴的持续运动距离无显著差异 ;19~ 34℃蜥蜴的跑道停顿次数较少 ,无显著差异 ,超过 34℃后显著增加。  相似文献   

6.
目的:观察"三伏贴"对稳定期慢性阻塞性肺疾病(COPD)患者系统炎症的影响,并评价其有效性。方法:收集稳定期慢性阻塞性肺疾病患者60例,按随机数字表随机分为"三伏贴"治疗组与安慰剂治疗组,每组30例;在常规西医治疗的基础上,分别给予"三伏贴"和安慰剂贴敷治疗,分别在入伏、初伏、中伏及末伏进行4次穴位贴敷,完成1疗程治疗者共56例。观察治疗前、1个疗程结束时、治疗后3个月、治疗后6个月血清炎症因子的变化。应用酶联免疫吸附试验(ELISA)检测血清白细胞介素-8(IL-8),免疫比浊法测定血清CRP,比较治疗前后两组间的炎症因子变化趋势,同时比较治疗前后两组间CAT评分(COPD assessment test,CAT)、呼吸困难评分(MRC)及生存质量评分。结果:"三伏贴"组、安慰剂组血清IL-8、CRP随时间变化趋势不同;其中试验组血清炎症因子随时间呈逐渐下降趋势,但治疗后6个月时炎症因子略有升高,而安慰剂组炎症因子水平呈逐渐升高趋势,治疗前试验组与安慰剂组炎症因子水平差异无统计学意义,治疗3个月后,两组患者IL-8、CRP水平差异即有统计学意义(P0.05)。试验组患者治疗后CAT评分、MRC评分较治疗前下降,生存质量评分升高,差异有统计学意义(P0.05),而安慰剂组患者在治疗后CAT评分、MRC评分较治疗前升高,差异有统计学意义(P0.05),生存质量评分下降,差异有统计学意义(P0.05)。同一时间点,试验组较安慰剂组CAT评分、MRC评分下降,生存质量评分明显升高,差异有统计学意义(P0.05)。结论:冬病夏治穴位敷帖可以改善COPD患者生存质量,缓解症状,其作用机理可能与降低系统炎症相关因子有关。  相似文献   

7.
刘佳  张春玲  徐德祥 《生物磁学》2014,(21):4123-4126
目的:观察“三伏贴”对稳定期慢性阻塞性肺疾病(COPD)患者系统炎症的影响,并评价其有效性。方法:收集稳定期慢性阻塞性肺疾病患者60 例,按随机数字表随机分为“三伏贴”治疗组与安慰剂治疗组,每组30 例;在常规西医治疗的基础上,分别给予“三伏贴”和安慰剂贴敷治疗,分别在入伏、初伏、中伏及末伏进行4 次穴位贴敷,完成1 疗程治疗者共56 例。观察治疗前、1 个疗程结束时、治疗后3 个月、治疗后6 个月血清炎症因子的变化。应用酶联免疫吸附试验(ELISA)检测血清白细胞介素-8(IL-8),免疫比浊法测定血清CRP,比较治疗前后两组间的炎症因子变化趋势,同时比较治疗前后两组间CAT 评分(COPD assessment test,CAT)、呼吸困难评分(MRC)及生存质量评分。结果:“三伏贴”组、安慰剂组血清IL-8、CRP随时间变化趋势不同;其中试验组血清炎症因子随时间呈逐渐下降趋势,但治疗后6 个月时炎症因子略有升高,而安慰剂组炎症因子水平呈逐渐升高趋势,治疗前试验组与安慰剂组炎症因子水平差异无统计学意义,治疗3个月后,两组患者IL-8、CRP水平差异即有统计学意义(P〈0.05)。试验组患者治疗后CAT 评分、MRC 评分较治疗前下降,生存质量评分升高,差异有统计学意义(P〈0.05),而安慰剂组患者在治疗后CAT 评分、MRC评分较治疗前升高,差异有统计学意义(P〈0.05),生存质量评分下降,差异有统计学意义(P〈0.05)。同一时间点,试验组较安慰剂组CAT 评分、MRC评分下降,生存质量评分明显升高,差异有统计学意义(P〈0.05)。结论:冬病夏治穴位敷帖可以改善COPD 患者生存质量,缓解症状,其作用机理可能与降低系统炎症相关因子有关。  相似文献   

8.
Dog diets may differ in their effectiveness of maintaining satiety after a meal. Consequently, sensations of hunger, feeding motivation, physical activity, and sensitivity to environmental stressors may be increased. Dietary fibre may be effective in prolonging postprandial satiety depending on type and inclusion level. This study evaluated the effect of fibre fermentability on behaviour in dogs. Sixteen healthy adult dogs were housed individually and fed a low-fermentable fibre (LFF) diet containing 8.5% cellulose or a high-fermentable fibre (HFF) diet containing 8.5% sugar beet pulp and 2% inulin. Dogs were fed two equal portions at 8:30 and 18:30 according to energy requirements. Behaviour of dogs in their home-cage was recorded and analyzed by instantaneous scan sampling (2 × 24 h with 15 min intervals) and focal sampling continuous recordings (10 min per animal per hour, from 9:00 until 18:00). Dogs were subjected to a behaviour test composed of the subtests open-field, sudden-silence, novel-object, and acoustic-startle. The behavioural responses of each dog were recorded. Scores for the scan and focal samples were expressed per clock hour and DIET × TIME effects were tested statistically using Residual Maximum Likelihood (REML). Data from the tests were examined using principal component analysis resulting in the compilation of two components. Data were tested statistically for DIET and DIET × SUBTEST effects using REML. Variables specific for the open-field and novel-object test were analyzed using analysis of variance. For the scans, a significant DIET × TIME effect was found for resting. At night and in the morning, HFF dogs rested more compared to LFF dogs, but they rested less between 14:00 and 17:00. For the continuous recordings, the main findings were a tendency for DIET × TIME effect for time spent resting with a pattern consistent with that for the scans. The interaction was significant for inactive-alert (lie with head up or sitting) with HFF-fed dogs having lower values around 10:00–11:00 and higher values hereafter. Finally, time spent tail wagging was significantly higher for LFF-fed dogs just before the evening meal that may indicate higher level of arousal. For the behaviour tests, no significant DIET or DIET × SUBTEST effects were detected. It is concluded that compared to the LFF diet, the HFF diet increased inactivity in kennelled beagle dogs likely through the prolongation of postprandial satiety. This effect did not change the reaction to stressful events in kennelled laboratory dogs. Enhanced susceptibility to environmental stressors at times of hunger in sensitive companion dogs may occur but requires further study.  相似文献   

9.
Corticosteroids are important factors in the maintenance of homeostasis in the brain. They are regulated via the interaction with two corticosteroid receptor systems—the mineralocorticoid (MR) and glucocorticoid receptor (GR). In the present study, we observed age-related changes in serum cortisol levels, and immunoreactivities and protein levels of MR and GR in the hippocampal CA1 region and dentate gyrus. The serum cortisol levels were significantly high (about twofold) in the aged group compared to that in the adult group. In the adult dog (2–3 years old), MR and GR immunoreactivity was detected in neurons in the pyramidal layer of the CA1 region, and in the granular and multiform layers of the dentate gyrus. In the aged dog (10–12 years old), MR immunoreactivity in the CA1 region was significantly decreased, especially, in the dentate multiform layer. In contrast, GR immunoreactivity in the aged dog was slightly decreased in the CA1 region and dentate gyrus. In the Western blot analysis, MR protein level in the aged dog was significantly lower compared to that of the adult dog; GR protein level in the aged dog was not significantly decreased. This result indicates that the reduction of MR immunoreactivity and protein level in the hippocampus of the aged dog may be associated with neural dysfunction in the aged hippocampus.  相似文献   

10.
Provisioning along pedestrian trails by tourists much increased the nutrient quality and patchiness of food (NqPF) for Tibetan macaques (Macaca thibetana) at Mt Emei in spring and summer. In the habitat at a temperate-subtropical transition zone, the macaque's NqPF could be ordered in a decreasing rank from spring-summer to autumn to winter. With the aid of a radio-tracking system, I collected ranging data on a multigroup community in three 70-day periods representing the different seasons in 1991–92. Rank-order correlation on the data show that with the decline of NqPF, the groups tended to increase days away from the trail, their effective range size (ERS), their exclusive area (EA) and the number of days spent in the EA, and reduced their group/community density and the ratio of the overlapped range to the seasonal range (ROR). In icy/snowy winter, the macaques searched for mature leaves slowly and carefully in the largest seasonal range with a considerable portion that was not used in other seasons. Of the responses, the ROR decreased with the reduction in group/community density; and the ERS was the function of both group size (+) and intergroup rank (–) when favorite food was highly clumped. All above responses were clearly bound to maximize foraging effectiveness and minimize energy expenditure, and their integration in term of changes in time and space leads to better understanding macaque ecological adaptability. Based on this study and previous work on behavioral and physiological factors, I suggest a unifying theory of intergroup interactions. In addition, as the rate of behavioral interactions was also related to the group density, Waser's (1976) gas model probably applies to behavioral, as well as spatial, data on intergroup interactions.  相似文献   

11.
Microsporidia are opportunistic pathogens that infect a wide range of invertebrates and vertebrates. To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing species specific polymerase chain reaction for the four most frequent human microsporidia. Two microsporidia species were recognized in 41 samples (14.5%). Encephalitozoon intestinalis was detected in 35 samples (12.4%) and it was the most common microsporidium. The second microsporidium, E. cuniculi, was identified in six (2.1%) of the samples. Sequence analysis of the intergenic spacer of the ribosomal ribonucleic acid (RNA) internal transcribed spacer (ITS) gene revealed the presence of three E. intestinalis haplotypes closely associated with each other. No polymorphic region was found among the ITS sequences of E. cuniculi isolates and they were characterized as genotype III. This study provides the first data on the zoonotic microsporidia species from dogs in Turkey.  相似文献   

12.
Similarities between age-related changes in the canine and human brain have resulted in the general acceptance of the canine brain as a model of human brain aging. The hippocampus is essentially required for intact cognitive ability and appears to be particularly vulnerable to the aging process. We observed changes in ionized calcium-binding adapter molecule 1 (Iba-1, a microglial marker) immunoreactivity and protein levels in the hippocampal dentate gyrus and CA1 region of adult (2-3 years) and aged (10-12 years) dogs. We also observed the interferon-gamma (IFN-gamma), a pro-inflammatory cytokine, protein levels in these groups. In the dentate gyrus and CA1 region of the adult dog, Iba-1 immunoreactive microglia were well distributed and their processes were highly ramified. However, in the aged dog, the processes of Iba-1 immunoreactive microglia were hypertrophied in the dentate gyrus. Moreover, Iba-1 protein level in the dentate gyrus in the aged dog was higher than in the adult dog. IFN-gamma expression was increased in the dentate gyrus homogenates of aged dogs than adult dogs. In addition, we found that some neurons were positive to Fluoro-Jade B (a marker for neuronal degeneration) in the dentate polymorphic layer, but not in the hippocampal CA1 region in the aged dog. These results suggest that Iba-1 immunoreactive microglia are hypertrophied in the dentate gyrus in the aged dog.  相似文献   

13.
海南变色树蜥个体发育中形态和食性的变化   总被引:10,自引:1,他引:10  
研究变色树蜥(Calotes versicolor)头,尾大小和食性在个体发育过程中的变化,以及头,尾大小两性异性的个体发生,成体体长(SVL)无显著的两性差异,两性异形主要表现为雄性个体有较大的头部(头长和头宽)和尾部。头,尾大小的两性异形在初生幼体就已存在,并随个体发育的进行变得更加显著。不同年龄组两性个体头长以及雌体头宽随SVL呈同速增长;雄性头宽随SVL呈异速增长,表现为雄性头宽的增长速率在个体发育过程中逐渐增大。头,尾部的相对大小在个体发育过程中有显著的变化,初生幼体头部相对较大,尾部相对较小,这种形态特征是胚胎优先保证生态学意义更为显著的部分(如头部)生长的结果,有利于初生幼体的早期生存和生长,不同性别和年龄组的变色树蜥摄入食物的种类及各种食物在摄入食物中所的比例有一定程度的差别,因而食物生态位宽度和重叠度有一定程度的差别,然而没有直接的证据表明头部大小的两性异形能导致两性食物生态位的明显分离。  相似文献   

14.
鱼精蛋白是一类天然的阳离子抗菌肽,具有广谱抑菌活性。鱼精蛋白主要是通过破坏细菌的细胞壁、细胞膜及改变细胞的渗透性等途径抑制甚至杀死细菌细胞。在鱼精蛋白抑制细菌的同时,细菌也产生多种机制对抗鱼精蛋白。温度、pH、阳离子和EDTA等多种理化因子影响鱼精蛋白对细菌的抑制效果。由于鱼精蛋白在抑菌防腐方面的众多优势,目前已成为非常有发展前景的食品防腐剂。  相似文献   

15.
野外研究于2002和2003年秋季在帽儿山地区进行。帽儿山地区秋季可供鸟类取食的肉质果植物种类繁多,黄檗(Phellodendronamurense)和山荆子(Malusbaccata)是最主要的两种植物。帽儿山地区迁徙性食果实鸟类共9种,包括夏候鸟和旅鸟5种,冬候鸟4种。主要的食果实鸟类是斑鸫(Turdusnaumanni)、灰背鸫(T.hortulorum)和北朱雀(Carpodacusroseus),前两种为食果肉鸟类,后一种是食种子鸟类。斑鸫主要取食黄檗果实,灰背鸫主要取食山荆子果实,北朱雀主要取食黄檗种子。食果实鸟类在秋季也捕食一些动物性食物。3种主要食果实鸟类的迁徙日期不同。灰背鸫最先迁离,斑鸫稍晚,北朱雀最后到此越冬。果实丰歉年对鸟类的迁徙有一定的影响。  相似文献   

16.
Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dog is the definitive host for N. caninum and can infect dairy cattle. The aim of this study is to determine the prevalence of Neospora oocysts in feces of dogs from dairy farms. A total of 174 fecal samples was collected from 89 farm dogs and 85 household dogs during 2006 and 2008. Fecal samples of dogs were microscopically examined for detecting Hammondia Neospora-like oocysts (HNLO) by Mini Parasep®SF fecal parasite concentrator. HNLO were microscopically detected in 4 fecal samples (2.2%). The fecal samples with HNLO were examined by N. caninum-specific PCR. Two of the samples were positive for N. caninum. The 2 positive fecal samples were selected for inoculation to calves. Two inoculated calves were seronegative by ELISA for 4 months post-infection. This is the first report of finding N. caninum DNA in feces of farm dogs in Mashhad area, Iran.  相似文献   

17.
Records on sheltered dogs were collected from 3 municipal dog shelters situated in different regions of the Czech Republic from 2010 to 2013. A total of 3,875 dogs were analyzed in this study. Among these, 1,614 dogs were subsequently reclaimed (lost dogs) and 2,261 dogs were abandoned and offered for adoption. The ratio of purebred dogs and crossbred dogs differed significantly when comparing lost (66.4% vs. 33.6%) and abandoned dogs (35.0% vs. 65.0%). The median time until lost dogs were reclaimed was 1 day, and it was not affected by purebred status. The median time until abandoned dogs were adopted was 23 days. In abandoned dogs, purebred status had a significant effect on the time the dog spent at the shelter before adoption. The median time until adoption for crossbred dogs was 27 days, whereas the median time until adoption for purebred dogs was 19 days. The breed group influenced the length of stay (LOS) in abandoned dogs. Small companion dogs had the shortest LOS (median = 15 days) and guard dogs had the longest LOS (median = 25 days).  相似文献   

18.
《Anthrozo?s》2013,26(4):557-568
ABSTRACT

In 2003, Japan passed the Act on Assistance Dogs for Persons with Disabilities, requiring public facilities, public transport, and private businesses serving the general public to allow access for people with physical impairments who use assistance dogs. One purpose of this law is to enable people with disabilities to lead independent and fulfilling lives as active members of society. In order to achieve this goal, the law requires public facilities such as national institutions and public transport, as well as private businesses serving the general public, such as hotels and restaurants, to allow access to people with physical impairment accompanied by assistance dogs. In 2007, the law was amended to stipulate acceptance to the workplace of people with a physical impairment accompanied by an assistance dog. We conducted a questionnaire survey of 6,062 business owners covered by the legislation, to ascertain the extent of knowledge of the law and acceptance of assistance dogs. In cases where the respondents had not employed any assistance dog users, they were also asked about the possibility of employing them in the future. The results indicate that assistance dog users are seldom accepted in the workplace, and familiarity with the law has not improved since the last survey in 2004, despite the 2007 amendment which is directly applicable to employers. Regarding the attitudes toward employment, most business owners answered that they would not or might not employ an assistance dog user. The level of knowledge of the law and the related financial support system differs among facility owners carrying out different types of business. Those positive toward employment tended to know much about the Act on Promotion of Smooth Transportation, etc. of Elderly Persons, Disabled Persons, etc. These findings suggest that there is a need for better dissemination of information about the obligation to accept assistance dog users in the workplace, of information about the users' obligations, how to cope with problems, and accessing consultation services.  相似文献   

19.
目的用不同浓度单宁酸(tannic acid,TA)处理后的花生喂养棕色田鼠(Lasiopodomys mandarinus)和小鼠(Mus musculus),研究单宁酸对两种鼠食物选择倾向、食物摄入量、蛋白质消化率的影响。方法本研究的设计如下:1)选择一批棕色田鼠和小鼠,单笼饲养,自由饮水。用正常花生饲喂二周实验用棕色田鼠和小鼠,使其适应这种食物;2)随机选出棕色田鼠和小鼠各10只(雌雄各半),单笼饲养。用经0%、5%、10%TA处理的花生饲喂供试鼠一周,观察和记录两种鼠对TA处理后花生的选择和取食特征;3)随机选出18只棕色田鼠和18只小鼠,单笼饲养,并各分为3组(每组均为6只),按两种鼠分别命名为对照组、低单宁酸组、高单宁酸组,并分别用经0%、5%和10%TA处理的花生饲喂一周,测定两种鼠的食物摄入量;同时收集粪便,用凯氏定氮仪测定粗蛋白含量,计算蛋白质消化率。结果1)棕色田鼠和小鼠均优先选择无TA食物(P〈0.001),二者间差异达显著性水平(P〈0.001);2)食物中的TA降低棕色田鼠和小鼠的相对日食量(P〈0.001)和食物中蛋白质的消化率,随TA含量的升高,棕色田鼠和小鼠的相对日食量和蛋白质消化率均显著下降。结论TA降低棕色田鼠和小鼠的食物摄入量和食物中蛋白质的消化率。TA对鼠类的食物摄入量和蛋白质消化率的影响有种间差异性,小鼠对TA的适应性更强。  相似文献   

20.
Nancy Rosenberger 《Ethnos》2013,78(3):339-360
Nations reverberate with the conundrums of unity and difference. Studies of food provide an effective way to understand this paradox as food indexes both rituals of the nation as national cuisine yet links with myriad and variable performances as it is grown, cooked and eaten. National foods enhance ethnic nationality in newly independent, increasingly authoritarian Uzbekistan where the focus is on national cuisine in part because of deprivation, rather than abundance. National cuisine is vulnerable to differences within a nation, because in the shared and embodied practices surrounding food, people make emotion-based evaluations of the nation-state. Uzbek governmental policies result in poverty, limited food production, little global food, and repression of pure Islamic practices, regions, and minority ethnic groups.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号