Northern Delta Lakes as Summertime CO2 Absorbers Within the Arctic Landscape

The vast majority of lakes examined worldwide emit CO₂ to the overlying atmosphere, through a process by which catchment-derived subsidies of terrigenous C, often in the form of dissolved organic carbon (DOC), augment within-lake CO₂ production above the level consumed via photosynthesis. We show that shallow, macrophyte-rich lakes of the Mackenzie Delta, western Canadian Arctic, do not follow this pattern. These lakes are strong summertime CO₂ absorbers, despite DOC concentrations at or above levels commonly shown to produce CO₂ emission. Paradoxically, CO₂ levels were lowest where DOC was greatest, in lakes which appear to be annual net CO₂ absorbers, and have poor hydrologic connection to the terrestrial landscape. CO₂ in these lakes is depleted by high macrophyte productivity, and although catchment-derived C subsidies are low, within-lake DOC generation appears to occur as a byproduct of macrophyte photosynthesis and evapoconcentration. Additionally, after accounting for DOC and macrophytes, lakes that were least connected to the larger terrestrial landscape remained weaker CO₂ absorbers, suggesting that CO₂ balance may also be affected by DOC quality, foodweb structure, or inputs of pCO₂-rich riverwater to connected lakes. In contrast, a small subset of Delta lakes that were strongly affected by permafrost melting were CO₂ emitters, suggesting future permafrost degradation could engender a change in the overall CO₂ balance of these lakes from near-CO₂ neutral over the ice-free season, to clear CO₂ emission. Our work suggests that the current paradigm of lakewater CO₂ regulation may need to specifically incorporate shallow, productive lakes, and those that are poorly connected to their surrounding landscape. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. ST, LL, and RH designed the study, ST performed research, ST analyzed data, ST, LL, and RH wrote the paper.     

Links between Terrestrial Primary Production and Bacterial Production and Respiration in Lakes in a Climate Gradient in Subarctic Sweden

We compared terrestrial net primary production (NPP) and terrestrial export of dissolved organic carbon (DOC) with lake water heterotrophic bacterial activity in 12 headwater lake catchments along an altitude gradient in subarctic Sweden. Modelled NPP declined strongly with altitude and annual air temperature decreases along the altitude gradient (6°C between the warmest and the coldest catchment). Estimated terrestrial DOC export to the lakes was closely correlated to NPP. Heterotrophic bacterial production (BP) and respiration (BR) were mainly based on terrestrial organic carbon and strongly correlated with the terrestrial DOC export. Excess respiration over PP of the pelagic system was similar to net emission of CO₂ in the lakes. BR and CO₂ emission made up considerably higher shares of the terrestrial DOC input in warm lakes than in cold lakes, implying that respiration and the degree of net heterotrophy in the lakes were dependant not only on terrestrial export of DOC, but also on characteristics in the lakes which changed along the gradient and affected the bacterial metabolization of allochthonous DOC. The study showed close links between terrestrial primary production, terrestrial DOC export and bacterial activity in lakes and how these relationships were dependant on air temperature. Increases in air temperature in high latitude unproductive systems might have considerable consequences for lake water productivity and release of CO₂ to the atmosphere, which are ultimately determined by terrestrial primary production.     

Diel and seasonal physico-chemical fluctuations in a small natural West African lake

SUMMARY. 1. The waters of Opi Lake were very dilute (15–24 μS cm⁻¹at 25°C) and somewhat acid (pH 6.0–6.5), probably a result of the highly leached lateritic soils in its small drainage basin.
2. Annual minimum water temperatures were recorded during the early dry season, not during the rainy season as has been observed in most other West African water bodies. This was attributed to the influence of the Harmattan winds on the lake and the absence of pronounced rainy season flooding, as might occur in reservoirs.
3. A diel cycle of daytime thermal stratification and complete or near-complete night-time destratification occurred throughout the year.
4. During the dry season, when prevailing winds were strong and lake depth shallow, night-time destratification was accompanied by deep circulation. In the rainy season, when prevailing winds were weak and lake depth was greatest, deep vertical circulation apparently did not take place, as evidenced by anoxia in near-bottom waters. This is the reverse of the pattern seen in many tropical lakes.     

Winter soil frost conditions in boreal forests control growing season soil CO2 concentration and its atmospheric exchange

The impact of changes in winter soil frost regime on soil CO₂ concentration and its atmospheric exchange in a boreal Norway spruce forest was investigated using a field‐scale soil frost manipulation experiment. The experiment comprised three treatments: deep soil frost, shallow soil frost and control plots (n= 3). Winter soil temperatures and soil frost distribution were significantly altered by the different treatments. The average soil CO₂ concentrations during the growing season were significantly lower in plots with deep soil frost than in plots with shallow soil frost. The average CO₂ soil–atmosphere exchange rate exhibited the same pattern, and differences in soil respiration rates among the treatments were statistically significant. Both the variation in soil CO₂ concentration and the CO₂ soil–atmosphere exchange rate could statistically be explained by the differences in the maximum soil frost depth during the previous winter. A response model for growing season soil respiration rates suggests that every 1 cm change in winter soil frost depth will change the emission rates by ca. 0.01 g CO₂ m^?2 day^?1, corresponding to 0.2–0.5% of the estimated net ecosystem productivity (NEP). This suggests that the soil frost regime has a significant influence on the C balance of the system, because interannual variations in soil frost up to 60 cm have been recorded at the site. We conclude that winter climate conditions can be important in controlling C balances in northern terrestrial ecosystems, and also that indirect effects of the winter season must be taken into account, because these can affect the prevailing conditions during the growing season.     

Linking forest fires to lake metabolism and carbon dioxide emissions in the boreal region of Northern Québec

Natural fires annually decimate up to 1% of the forested area in the boreal region of Québec, and represent a major structuring force in the region, creating a mosaic of watersheds characterized by large variations in vegetation structure and composition. Here, we investigate the possible connections between this fire‐induced watershed heterogeneity and lake metabolism and CO₂ dynamics. Plankton respiration, and water–air CO₂ fluxes were measured in the epilimnia of 50 lakes, selected to lie within distinct watershed types in terms of postfire terrestrial succession in the boreal region of Northern Québec. Plankton respiration varied widely among lakes (from 21 to 211 μg C L^?1 day^?1), was negatively related to lake area, and positively related to dissolved organic carbon (DOC). All lakes were supersaturated in CO₂ and the resulting carbon (C) flux to the atmosphere (150 to over 3000 mg C m² day^?1) was negatively related to lake area and positively to DOC concentration. CO₂ fluxes were positively related to integrated water column respiration, suggesting a biological component in this flux. Both respiration and CO₂ fluxes were strongly negatively related to years after the last fire in the basin, such that lakes in recently burnt basins had significantly higher C emissions, even after the influence of lake size was removed. No significant differences were found in nutrients, chlorophyll, and DOC between lakes in different basin types, suggesting that the fire‐induced watershed features influence other, more subtle aspects, such as the quality of the organic C reaching lakes. The fire‐induced enhancement of lake organic C mineralization and C emissions represents a long‐term impact that increases the overall C loss from the landscape as the result of fire, but which has never been included in current regional C budgets and future projections. The need to account for this additional fire‐induced C loss becomes critical in the face of predictions of increasing incidence of fire in the circumboreal landscape.     

Carbon Cycling of Lake Kivu (East Africa): Net Autotrophy in the Epilimnion and Emission of CO2 to the Atmosphere Sustained by Geogenic Inputs

We report organic and inorganic carbon distributions and fluxes in a large (>2000 km²) oligotrophic, tropical lake (Lake Kivu, East Africa), acquired during four field surveys, that captured the seasonal variations (March 2007–mid rainy season, September 2007–late dry season, June 2008–early dry season, and April 2009–late rainy season). The partial pressure of CO₂ (pCO₂) in surface waters of the main basin of Lake Kivu showed modest spatial (coefficient of variation between 3% and 6%), and seasonal variations with an amplitude of 163 ppm (between 579±23 ppm on average in March 2007 and 742±28 ppm on average in September 2007). The most prominent spatial feature of the pCO₂ distribution was the very high pCO₂ values in Kabuno Bay (a small sub-basin with little connection to the main lake) ranging between 11213 ppm and 14213 ppm (between 18 and 26 times higher than in the main basin). Surface waters of the main basin of Lake Kivu were a net source of CO₂ to the atmosphere at an average rate of 10.8 mmol m⁻² d⁻¹, which is lower than the global average reported for freshwater, saline, and volcanic lakes. In Kabuno Bay, the CO₂ emission to the atmosphere was on average 500.7 mmol m⁻² d⁻¹ (∼46 times higher than in the main basin). Based on whole-lake mass balance of dissolved inorganic carbon (DIC) bulk concentrations and of its stable carbon isotope composition, we show that the epilimnion of Lake Kivu was net autotrophic. This is due to the modest river inputs of organic carbon owing to the small ratio of catchment area to lake surface area (2.15). The carbon budget implies that the CO₂ emission to the atmosphere must be sustained by DIC inputs of geogenic origin from deep geothermal springs.     

Frozen Cropland Soil in Northeast China as Source of N2O and CO2 Emissions

Agricultural soils are important sources of atmospheric N₂O and CO₂. However, in boreal agro-ecosystems the contribution of the winter season to annual emissions of these gases has rarely been determined. In this study, soil N₂O and CO₂ fluxes were measured for 6 years in a corn-soybean-wheat rotation in northeast China to quantify the contribution of wintertime N₂O and CO₂ fluxes to annual emissions. The treatments were chemical fertilizer (NPK), chemical fertilizer plus composted pig manure (NPKOM), and control (Cont.). Mean soil N₂O fluxes among all three treatments in the winter (November–March), when soil temperatures are below −7°C for extended periods, were 0.89–3.01 µg N m⁻² h⁻¹, and in between the growing season and winter (October and April), when freeze-thaw events occur, 1.73–5.48 µg N m⁻² h⁻¹. The cumulative N₂O emissions were on average 0.27–1.39, 0.03–0.08 and 0.03–0.11 kg N₂O_–N ha⁻¹ during the growing season, October and April, and winter, respectively. The average contributions of winter N₂O efflux to annual emissions were 6.3–12.1%. In all three seasons, the highest N₂O emissions occurred in NPKOM, while NPK and Cont. emissions were similar. Cumulative CO₂ emissions were 2.73–4.94, 0.13–0.20 and 0.07–0.11 Mg CO₂-C ha⁻¹ during growing season, October and April, and winter, respectively. The contribution of winter CO₂ to total annual emissions was 2.0–2.4%. Our results indicate that in boreal agricultural systems in northeast China, CO₂ and N₂O emissions continue throughout the winter.     

Interannual variation and climatic regulation of the CO2 emission from large boreal lakes

We studied the interannual variation of surface water partial pressure of CO₂ (pCO₂) and the CO₂ emissions from the 37 large Finnish lakes linking them to the water quality, catchment and climate attributes in 1996–2001. The lake water CO₂ was measured three times a year in the study lakes in 1998 and 1999 and for the rest of the years the CO₂ was modeled by measured alkalinity. The median annual CO₂ emission to the atmosphere ranged between 1.49 and 2.29 mol m^?2 a^?1. The annual CO₂ emission followed closely the annual precipitation pattern with the highest emission during the years when the precipitation was highest (r²=0.81–0.97, P<0.05). There was a strong negative correlation (r²=0.50–0.82, P<0.001) between O₂ and CO₂ saturation in the lake water during stratification suggesting effective decomposition of organic matter in the lakes. Furthermore, total phosphorus and the proportion of agricultural land in the catchment had significant positive correlations with CO₂ saturation.     

Land use and season affect fluxes of CO2, CH4, CO,N2O,H2 and isotopic source signatures in Panama: evidence from nocturnal boundary layer profiles

Conversion of tropical rainforests to pastures and plantations is associated with changes in soil properties and biogeochemical cycling, with implications for carbon cycling and trace gas fluxes. The stable isotopic composition of ecosystem respiration (δ¹³C_R and δ¹⁸O_R) is used in inversion models to quantify regional patterns of CO₂ sources and sinks, but models are limited by sparse measurements in tropical regions. We measured soil respiration rates, concentrations of CO₂, CH₄, CO, N₂O and H₂ and the isotopic composition of CO₂, CH₄ and H₂ at four heights in the nocturnal boundary layer (NBL) above three common land‐use types in central Panama, during dry and rainy seasons. Soil respiration rates were lowest in Plantation (average 3.4 μmol m^?2 s^?1), highest in Pasture (8.3 μmol m^?2 s^?1) and intermediate in Rainforest (5.2 μmol m^?2 s^?1). δ¹³C_R closely reflected land use and increased during the dry season where C₃ vegetation was present. δ¹⁸O_R did not differ by land use but was lower during the rainy than the dry season. CO₂ was correlated with other species in approximately half of the NBL profiles, allowing us to estimate trace gas fluxes that were generally within the range of literature values. The Rainforest soil was a sink for CH₄ but emissions were observed in Pasture and Plantation, especially during the wet season. N₂O emissions were higher in Pasture and Plantation than Rainforest, contrary to expectations. Soil H₂ uptake was highest in Rainforest and was not observable in Pasture and Plantation during the wet season. We observed soil CO uptake during the dry season and emissions during the wet season across land‐use types. This study demonstrated that strong impacts of land‐use change on soil–atmosphere trace gas exchange can be detected in the NBL, and provides useful observational constraints for top‐down and bottom‐up biogeochemistry models.     

Sedimentation in Boreal Lakes—The Role of Flocculation of Allochthonous Dissolved Organic Matter in the Water Column

We quantified sedimentation of organic carbon in 12 Swedish small boreal lakes (<0.48 km²), which ranged in dissolved organic carbon (DOC) from 4.4 to 21.4 mg C l⁻¹. Stable isotope analysis suggests that most of the settling organic matter is of allochthonous origin. Annual sedimentation of allochthonous matter per m² lake area was correlated to DOC concentration in the water (R ² = 0.41), and the relationship was improved when sedimentation data were normalized to water depth (R ² = 0.58). The net efflux of C as CO₂ from the water to the atmosphere was likewise correlated to DOC concentration (R ² = 0.52). The losses of organic carbon from the water column via mineralization to CO₂ and via sedimentation were approximately of equal importance throughout the year. Our results imply that DOC is a precursor of the settling matter, resulting in an important pathway in the carbon cycle of boreal lakes. Thus, flocculation of DOC of terrestrial origin and subsequent sedimentation could lead to carbon sequestration by burial in lake sediments.     

Distribution of dissolved organic carbon in lakes of different trophic types

The distributions of dissolved organic carbon (DOC) in the warm season were elucidated in ten lakes of different trophic types in Japan, Russia, and China. DOC showed similar vertical distributions in all the lakes in summer when thermal stratification occurred. DOC in the epilimnion was higher than the value of 0.8mgCl^–1 found in the hypolimnion. In three Japanese lakes, hypolimnion DOC was negatively correlated with apparent oxygen utilization (AOU), reflecting the net oxidation of DOC using the dissolved oxygen in lake water. The DOC:O₂ ratios (0.115–0.179), calculated by the slopes of the regression lines of DOC versus AOU in hypolimnion water, were as low as those of deep-sea water, which indicates low bioavailability of lake water DOC for heterotrophic bacteria. DOC and conductivity did not correlate well except in two Japanese lakes: one showed a positive correlation and the other a negative correlation, indicating DOC loading from the inflowing rivers. Eutrophic lakes tended to have higher DOC values than meso- and oligotrophic lakes, and DOC values in the surface water negatively correlated with Secchi depths.     

Biogeochemistry of organic carbon, CO2, CH4, and trace elements in thermokarst water bodies in discontinuous permafrost zones of Western Siberia

Active processes of permafrost thaw in Western Siberia increase the number of soil subsidencies, thermokarst lakes and thaw ponds. In continuous permafrost zones, this process promotes soil carbon mobilisation to water reservoirs, as well as organic matter (OM) biodegradation, which produces a permanent flux of carbon dioxide (CO₂) to the atmosphere. At the same time, the biogeochemical evolution of aquatic ecosystems situated in the transition zone between continuous permafrost and permafrost-free terrain remains poorly known. In order to better understand the biogeochemical processes that occur in thaw ponds and lakes located in discontinuous permafrost zones, we studied ~30 small (1–100,000 m²) shallow (<1 m depth) lakes and ponds formed as a result of permafrost subsidence and thaw of the palsa bog located in the transition zone between the tundra and forest-tundra (central part of Western Siberia). There is a significant increase in dissolved CO₂ and methane (CH₄) concentration with decreasing water body surface area, with the largest supersaturation with respect to atmospheric CO₂ and CH₄ in small (<100 m²) permafrost depressions filled with thaw water. Dissolved organic carbon (DOC), conductivity, and metal concentrations also progressively increase from large lakes to thaw ponds and depressions. As such, small water bodies with surface areas of 1–100 m² that are not accounted for in the existing lake and pond databases may significantly contribute to CO₂ and CH₄ fluxes to the atmosphere, as well as to the stocks of dissolved trace elements and organic carbon. In situ lake water incubation experiments yielded negligible primary productivity but significant oxygen consumption linked to the mineralisation rate of dissolved OM by heterotrophic bacterioplankton, which produce a net CO₂ flux to the atmosphere of 5 ± 2.5 mol C m² year^?1. The most significant result of this study, which has long-term consequences on our prediction of aquatic ecosystem development in the course of permafrost degradation is CO₂, CH₄, and DOC concentrations increase with decreasing lake age and size. As a consequence, upon future permafrost thaw, the increase in the number of small water bodies, accompanied by the drainage of large thermokarst lakes to the hydrological network, will likely favour (i) the increase of DOC and colloidal metal stocks in surface aquatic systems, and (ii) the enhancement of CO₂ and CH₄ fluxes from the water surface to the atmosphere. According to a conservative estimation that considers that the total area occupied by water bodies in Western Siberia will not change, this increase in stocks and fluxes could be as high as a factor of ten.     

Gross primary production controls the subsequent winter CO2 exchange in a boreal peatland

In high‐latitude regions, carbon dioxide (CO₂) emissions during the winter represent an important component of the annual ecosystem carbon budget; however, the mechanisms that control the winter CO₂ emissions are currently not well understood. It has been suggested that substrate availability from soil labile carbon pools is a main driver of winter CO₂ emissions. In ecosystems that are dominated by annual herbaceous plants, much of the biomass produced during the summer is likely to contribute to the soil labile carbon pool through litter fall and root senescence in the autumn. Thus, the summer carbon uptake in the ecosystem may have a significant influence on the subsequent winter CO₂ emissions. To test this hypothesis, we conducted a plot‐scale shading experiment in a boreal peatland to reduce the gross primary production (GPP) during the growing season. At the growing season peak, vascular plant biomass in the shaded plots was half that in the control plots. During the subsequent winter, the mean CO₂ emission rates were 21% lower in the shaded plots than in the control plots. In addition, long‐term (2001–2012) eddy covariance data from the same site showed a strong correlation between the GPP (particularly the late summer and autumn GPP) and the subsequent winter net ecosystem CO₂ exchange (NEE). In contrast, abiotic factors during the winter could not explain the interannual variation in the cumulative winter NEE. Our study demonstrates the presence of a cross‐seasonal link between the growing season biotic processes and winter CO₂ emissions, which has important implications for predicting winter CO₂ emission dynamics in response to future climate change.     

Sediment respiration and lake trophic state are important predictors of large CO2 evasion from small boreal lakes

We show that sediment respiration is one of the key factors contributing to the high CO₂ supersaturation in and evasion from Finnish lakes, and evidently also over large areas in the boreal landscape, where the majority of the lakes are small and shallow. A subpopulation of 177 randomly selected lakes (<100 km²) and 32 lakes with the highest total phosphorus (P_tot) concentrations in the Nordic Lake Survey (NLS) data base were sampled during four seasons and at four depths. Patterns of CO₂ concentrations plotted against depth and time demonstrate strong CO₂ accumulation in hypolimnetic waters during the stratification periods. The relationship between O₂ departure from the saturation and CO₂ departure from the saturation was strong in the entire data set (r²=0.79, n=2 740, P<0.0001). CO₂ concentrations were positively associated with lake trophic state and the proportion of agricultural land in the catchment. In contrast, CO₂ concentrations negatively correlated with the peatland percentage indicating that either input of easily degraded organic matter and/or nutrient load from agricultural land enhance degradation. The average lake‐area‐weighted annual CO₂ evasion based on our 177 randomly selected lakes and all Finnish lakes >100 km² ( Rantakari & Kortelainen, 2005 ) was 42 g C m^?2 LA (lake area), approximately 20% of the average annual C accumulation in Finnish forest soils and tree biomass (covering 51% of the total area of Finland) in the 1990s. Extrapolating our estimate from Finland to all lakes of the boreal region suggests a total annual CO₂ evasion of about 50 TgC, a value upto 40% of current estimates for lakes of the entire globe, emphasizing the role of small boreal lakes as conduits for transferring terrestrially fixed C into the atmosphere.     

Release of CO2 and CH4 from lakes and drainage ditches in temperate wetlands

Shallow fresh water bodies in peat areas are important contributors to greenhouse gas fluxes to the atmosphere. In this study we determined the magnitude of CH₄ and CO₂ fluxes from 12 water bodies in Dutch wetlands during the summer season and studied the factors that might regulate emissions of CH₄ and CO₂ from these lakes and ditches. The lakes and ditches acted as CO₂ and CH₄ sources of emissions to the atmosphere; the fluxes from the ditches were significantly larger than the fluxes from the lakes. The mean greenhouse gas flux from ditches and lakes amounted to 129.1 ± 8.2 (mean ± SE) and 61.5 ± 7.1 mg m^?2 h^?1 for CO₂ and 33.7 ± 9.3 and 3.9 ± 1.6 mg m^?2 h^?1 for CH₄, respectively. In most water bodies CH₄ was the dominant greenhouse gas in terms of warming potential. Trophic status of the water and the sediment was an important factor regulating emissions. By using multiple linear regression 87% of the variation in CH₄ could be explained by PO₄ ^3? concentration in the sediment and Fe²⁺ concentration in the water, and 89% of the CO₂ flux could be explained by depth, EC and pH of the water. Decreasing the nutrient loads and input of organic substrates to ditches and lakes by for example reducing application of fertilizers and manure within the catchments and decreasing upward seepage of nutrient rich water from the surrounding area will likely reduce summer emissions of CO₂ and CH₄ from these water bodies.     

Phytoplankton and Primary Production of Lakes in the Matamek Watershed,Quebec

The productivity of three Shield lakes on Quebec's North Shore was found to be comparable with that of the most oligotrophic lakes known. Factors contributing to this condition may be that the moderately sized lakes of this study are deep relative to their surface areas, highly stained by humic substances, of very low conductivity, and contain little dissolved CO₂. Standing crops of phytoplankton are very low, never exceeding 600 mg/m³. Chrysophyceae are dominant over all other classes of algae in both biomass and numbers, comprising approximately 50% of the fresh weight throughout the season. The abundance of micro-flagellates implies a large surface area to volume ratio and the ability to move are an advantage in a nutrient poor environment. Much of the phytoplankton present may grow heterotrophically and energy additions from the terrestrial environment may be extremely important to the productivity of northern lakes when photosynthetic production is not significant.     

The influence of rising tropospheric carbon dioxide and ozone on plant productivity

Human activities result in a wide array of pollutants being released to the atmosphere. A number of these pollutants have direct effects on plants, including carbon dioxide (CO₂), which is the substrate for photosynthesis, and ozone (O₃), a damaging oxidant. How plants respond to changes in these atmospheric air pollutants, both directly and indirectly, feeds back on atmospheric composition and climate, global net primary productivity and ecosystem service provisioning. Here we discuss the past, current and future trends in emissions of CO₂ and O₃ and synthesise the current atmospheric CO₂ and O₃ budgets, describing the important role of vegetation in determining the atmospheric burden of those pollutants. While increased atmospheric CO₂ concentration over the past 150 years has been accompanied by greater CO₂ assimilation and storage in terrestrial ecosystems, there is evidence that rising temperatures and increased drought stress may limit the ability of future terrestrial ecosystems to buffer against atmospheric emissions. Long‐term Free Air CO₂ or O₃ Enrichment (FACE) experiments provide critical experimentation about the effects of future CO₂ and O₃ on ecosystems, and highlight the important interactive effects of temperature, nutrients and water supply in determining ecosystem responses to air pollution. Long‐term experimentation in both natural and cropping systems is needed to provide critical empirical data for modelling the effects of air pollutants on plant productivity in the decades to come.     

Long-Term CO2 Variability in Two Shallow Tropical Lakes Experiencing Episodic Eutrophication and Acidification Events

Here we report the long-term (13-year) dynamics of surface pCO₂ and its response to episodic eutrophication and acidification events in two contrasting tropical coastal lakes, one clear-water and the other humic. A short-term nutrient addition experiment was also conducted in mesocosms in the humic lake where in situ eutrophication was moderate. Our objective was to elucidate the response of pCO₂ to interannual changes in key limnological conditions, such as nutrient concentrations and pH. The humic waters showed a median pCO₂ almost ninefold higher across the 13-year study than the clear waters, supporting pCO₂ values about tenfold above atmospheric equilibrium. Eutrophication of the clear-water lake resulted in a decrease in pCO₂ to median values below atmospheric equilibrium, producing a strong sink for atmospheric CO₂. In contrast, pCO₂ increased by over tenfold in both lakes during the acidification phase, resulting in very large CO₂ emissions to the atmosphere. Experimental nutrient additions in the humic lake showed a strong persistence of high pCO₂. The extreme variability in pCO₂ observed here might be a characteristic of tropical lakes and may have important consequences for regional carbon budgets.     

Lakes as nitrous oxide sources in the boreal landscape

Estimates of regional and global freshwater N₂O emissions have remained inaccurate due to scarce data and complexity of the multiple processes driving N₂O fluxes the focus predominantly being on summer time measurements from emission hot spots, agricultural streams. Here, we present four‐season data of N₂O concentrations in the water columns of randomly selected boreal lakes covering a large variation in latitude, lake type, area, depth, water chemistry, and land use cover. Nitrate was the key driver for N₂O dynamics, explaining as much as 78% of the variation of the seasonal mean N₂O concentrations across all lakes. Nitrate concentrations varied among seasons being highest in winter and lowest in summer. Of the surface water samples, 71% were oversaturated with N₂O relative to the atmosphere. Largest oversaturation was measured in winter and lowest in summer stressing the importance to include full year N₂O measurements in annual emission estimates. Including winter data resulted in fourfold annual N₂O emission estimates compared to summer only measurements. Nutrient‐rich calcareous and large humic lakes had the highest annual N₂O emissions. Our emission estimates for Finnish and boreal lakes are 0.6 and 29 Gg N₂O‐N/year, respectively. The global warming potential of N₂O from lakes cannot be neglected in the boreal landscape, being 35% of that of diffusive CH₄ emission in Finnish lakes.     

Dissolved Organic Carbon as Major Environmental Factor Affecting Bacterioplankton Communities in Mountain Lakes of Eastern Japan

Relationships between environmental factors and bacterial communities were investigated in 41 freshwater lakes located in mountainous regions of eastern Japan. Bacterioplankton community composition (BCC) was determined by polymerase chain reaction-denaturing gradient gel electrophoresis of the 16S rRNA gene and then evaluated on the basis of physicochemical and biological variables of the lakes. Canonical correspondence analysis revealed that BCC of oligotrophic lakes was significantly influenced by dissolved organic carbon (DOC) content, but its effect was not apparent in the analysis covering all lakes including mesotrophic and eutrophic ones. The generalized linear model showed the negative association of DOC on the taxon richness of bacterioplankton communities. DOC was positively correlated with the catchment area per lake volume, suggesting that a large fraction of DOC supplied to the lake was derived from terrestrial sources. These results suggest that allochthonous DOC has a significant effect on bacterioplankton communities especially in oligotrophic lakes. The genus Polynucleobacter was detected most frequently. The occurrence of Polynucleobacter species was positively associated with DOC and negatively associated with total phosphorus (TP) levels. In addition, TP had a stronger effect than DOC, suggesting that oligotrophy is the most important factor on the occurrence of this genus.