Notes on Syringa meyeri Schneid. and Its Confused Species

By comparing the type specimens, Syringa spontanea is distinguished from S. meyeri by the small suborbicular lamina palmately 5-veined and the dense pubescence onterete branchlets. Therefore, it is more consistent to recognize S.spontanea as a distinct species.     

Pollen morphology of Gymnadenia R. Br. (Orchidaceae) and its taxonomic significance

Pollen morphology of five species from China in the genus Gymnadenia R. Br. (Orchidaceae) was investigated in this work. Pollen characteristics of this genus were compared to those of its closely related genus, Neottianthe Schltr. It is found that there are some differences of pollen morphology between the two genera, e.g.: (A) Pollen massulae are mostly irregular less frequently trigonous-conical in Gymnadenia, while mainly trigenuus-conical less frequently irregular in Neottianthe; (B) Ornamentation of pollen exine surface is usually indistinct, but microperforate, microrugulate, microfoveolate or both microperforate and microfossulate in high magnification in Gymnadenia, but more distinct in Neottianthe. Exine ornamentation of this genus can be divided into five types, ( 1 ) almost smooth, or very obscurely fosset, (2) perforate, (3) fossulate or foveolate, (4) reticuloid, (5) typically reticulate. (C) the surface of pollen massulae in Gymnadenia possess viscin thread, but that in Neottianthe does not. The result of this work supports the separation ofNeottianthe from Gymnadenia as an independent genus.     

Studies on the Genus Chondrus (Gigartinaceae) from Dalian,China

Two species of Chondrus, C. armatus (Harv.)Okam. and C. yendoi Yamada et Mikami from the coast of Dalian, Liaoning, China, are reported in this paper. C. yendoi is new to China.     

A subspecific taxonomic study on Asplenium trichomanes L. from China

With the evidence from palynological, cytological, ecological and morphological data, the subspecific taxonomy of Asplenium trichomanes L. from China is carried out. Four subspecies and one variety, i.e.A. trichomanes L. ssp. trichomanes, A. trichomanes L. ssp. inexpectans Lovis, A. trichomanes L. ssp. quadrivalens D. E. Meyer emend. Lovis, A. trichomanes L. ssp. pachyrachis (Christ) Lovis et Reichst. and A. trichomanes L. var. harovii Moore emend. Midle are recognized from China. The distribution of each subspecies and variety is also presented. Some fragments of a type specimen named as A. trichomanes L. var. centrochinense Christ in PE are found to be different from the known taxa of A. trichomanes L. complex both morphologically andcytologically, and therefore are regarded representing a new species.     

SARS病毒与其他冠状病毒的基因组比较

本文利用生物信息学方法比较SARS病毒和其他冠状病毒基因组.通过数据库搜索,找出与SARS病毒基因组相似的核酸或蛋白质序列,并对相似序列进行比对,分析它们的共性和差异.结果表明,SARS病毒在基因组的组织上及结构蛋白质方面与现有冠状病毒有比较大的相似性,SARS病毒基因组与冠状病毒基因组相关.但是,SARS病毒基因组还存在一些特异性序列,ORF1a和S蛋白(特别是S1)的变化以及SARS-CoV特异性的非结构蛋白可能是SARS发病机理与传染特性区别于其他冠状病毒的分子基础.在全基因组水平上进行核酸单词出现频率分析,结果表明,SARS病毒远离已知的其他冠状病毒,单独成为一类.     

Paeonia rockii and Its One New Subspecies from Mt. Taibai,Shaanxi of China

The history of exploration and classification of Paeonia rockii is reviewed in the present paper. Two allopatric subspecies in this species are recognized. The new subspecies, subsp, taibaishanica Hong, found on northern slope of the Qingling Range has leaflets ovate or rounded, totally or mostly lobed. P. rockii subsp, linyanshanii T. Hong et G. L. Ostiis found to be a superfluous name of P. rockii subsp, rockii.     

蛩蠊目昆虫在中国的发现及一新种记述

蛩蠊目昆虫地位古老,是昆虫纲的活化石,巳知种类不多,分布区狭窄,我国一直没有报道.作者在长白山采得一雄虫,系一新种,是该目昆虫在我国的新发现.     

Karyomorphology of the genus Pomatosace Maxin. (Primulaceae)

Reported in this paper was the karyomorphology of the monotypic genus Pomatosace Maxim. The interphase nuclei and prophase chromosomes of P. filicula Maxim. were categorized to be complex chromocenter type and interstitial type respectively; themetaphase chromosomes were counted to be 2n = 20, ranging in length from 6.4µm to 4.1µm; the karyotype was formulated as 2n= 18m + 2sm, with the karyotype asymmetry belonging to 2A. The similar karyomorphological characteristics of interphase nuclei and　prophase chromosomes between Pomatosace and Androsace, together with the similar sizeand morphology of their metaphase chromosomes, support the viewpoint that they are closelyrelated.     

Additional Notes on a Chrysosplenium L. (Saxifragaceae) from Anhui,China

A new variety Chrysosplenium japonicum var. cuneifolium from Anhui, China, is described, and the species Chrysosplenium delavayi is found new to Anhui. The genusChrysosplenium L. of Anhui is additionally recounted.     

Karyotype Evidence for Distinguishing Between Hemerocallis esculenta and H. middendorfii

Hemerocallis dumortierii, H. esculenta and H. middendorfii were considered to belong to a biological complex, and were treated as different varieties of the same species, H. dumortierii. The present authors observed and compared the karyotypes of H. esculenta and H. middendorfii. Two plant samples for each of the species were studied. One sample of H. esculenta was from Wuán County, Hebei Province, at an altitute of 1000 m, the other from Shennongjia, Hubei Province, altitute 2100 m. The two samples of H. middendorfii were respectively from Antu County, at an altitute of 700 m, and Tonghua, altitute 500 m, in Jilin Province. The karyotypes are 2n＝2x=22=12m+8sm+2T for H. esculenta, while 2n＝2x＝22=10m+6sm+4st+2T for H. middendorfii. The karyotypical difference between the two taxa was obviously not intraspecific but interspecific. Based on the karyotype data as well as the morphological and phytogeographical data available, it is considered that H. esculenta and H. middendorfii should be recognized as different species, instead of different varieties of the same species as in literature, and that H. mid-dendorfii is more advanced than H. esculenta.     

Notes on the genus Clematis (Ranunculaceae) (Ⅲ)

(1) The evolutionary trends of sepals and stamens of the genus Clematis are discussed; (2) New classifications for sect. Cheiropsis DC. and sect. Aspidanthera Spach are proposed; (3) One subsection, 13 series, 5 species and 4 varieties are described as new; (4) Six new ranks and2 new combinations are made.     

Chemical Characteristics of Oleoresin from Pinus bungeana Zucc. and Its Taxonomic Implication

The chemical characteristrics of oleoresin from Pinus bungeana Zucc. was studied. It contains 25 contents. The amount of its monoterpenes is about 47.0% and that of β-pinene is as high as 24.8%. The chemical compositions of oleoresins of 16 species from sub-gen. Strobus and subgen. Pinus were also analyzed by GC and GC-MS. The amounts of isopimaric acid, palustric acid/levopimaric acid, lambertianic acid and abietic acid in P. bungeana oleorensin differ from that of subgen. Strobus, and is similar to that of subgen.Pinus .     

Taxonomic notes on the genus Illicium Linn.

Taxonomic notes of the genus Illicium Linn. are presented based mainly on herbarium studies involving more than 10 thousand speciemens from 120 herbaria worldwide, Three new combinations are made, viz, I. fargesii Finet & Gagnep. Subsp. szechuanensis (Cheng) Q. Lin, I. parvifolium Merr. Subsp. oligandrum (Merr. & Chun)Q. Lin and I. micranhum Dunn subsp. tsangii (A. C. Smith)Q. Lin, and thirteen species, six subspecies, three varieties and two forms are reduced to synonyms. Thirty-four species, three subspecies and one variety are recognized inthis genus.     

Taxonomic notes on some species of Aconitum L. (Ranunculaceae) from Yunnan,China

In this paper, some species of Aconitum L. (Ranunculaceae) from Yunnan are taxonomically revised. Twenty-seven species, twenty-three varieties and three forms are reduced to synonyms; the specific status of A. fengii is restored, and one variety, A. fengiivar. crispulum Q. E. Yang is described as new.     

养殖贻贝中观察到一种球形病毒

用电镜负染方法在发病的贻贝内脏团、鳃、外套膜组织匀浆中观察到一种球形病毒粒子,其直径为80～200nm,具囊膜.上述组织超薄切片发现在其结缔组织细胞质中均存在该病毒粒子,带双层囊膜,有"封入体"结构,未见包涵体.在未发病的杂色蛤、香螺、牡蛎等内脏团中也观察到少量同种病毒粒子.用带病毒贻贝组织匀浆投喂和划伤感染不带病毒的绣凹螺,使之成为带病毒状态.采用蔗糖梯度离心法,25 000 r/min,离心3h,可于20%～30%蔗糖层间获得病毒沉淀带.     

腹腔注射重组腺病毒诱导的免疫反应

为研究重组腺病毒接种实验动物后的免疫反应性,利用RT-PCR方法,从HAV的RNA中克隆了结构蛋白基因插入穿梭质粒pXCX2Not I,通过磷酸钙-DNA共沉淀技术,将复制缺陷型腺病毒载体与线形化的pXCX2 CMV-HAV共转染293细胞.一系列检测方法证明产生了重组腺病毒rAdHAV.纯化后的rAdHAV滴度为1×109TCID50/mL,腹腔注射免疫昆明种小白鼠后,可诱导产生抗HAV IgG和HAV中和抗体.复制缺陷型腺病毒可作为发展基因工程病毒疫苗载体的有效系统.