多歧苏铁的修订

中国特有种。产云南东南部元江流域,个旧、蒙自、屏边与河口交界处,海拔200-1000     

多歧苏铁的补述

多歧苏铁 独把铁,独脚铁(云南屏边) 图版1 Cycas multipinnata C. J. Chen et S. Y. Yang in Acta Phytotax. Sin. 32(3): 239.1994. Plate 1 Trunk 20—40 cm tall, 10—20 cm in diam., brown-grey. Leaves 1, rarely 2, termi-     

Karyotype Analysis of Cycas panzhihuaensis L. Zhou et S. Y. Yang

This paper reports chromosome number and karyotype analysis of Cycas panzhihuaensis endemic to China. The material was collected from Dukou, Sichuan. It is a diploid species, with 2n=22=2m+4sm+4st+l2t. The karyotype of Cycas panzhihuaensis is different from that of the other species of the genus Cycas, which was known to be 2n=4m+8st+10t. The former is a new karyotype in the genus. The authors briefly discuss karyotype evolution of the genus Cycas in this papar.     

德保苏铁居群特征及保护措施

报道了德保苏铁(CycasdebaoensisY.C.ZhongetC.J.Chen)的居群结构调查情况,并与百色地区林业局1998年的调查结果进行比较。结果表明,德保苏铁分布区狭窄,仅产于广西壮族自治区德保县扶平乡约15.3hm2的石灰岩山坡;由于人为原因,其数量已从1998年的2000多株锐减到2001年的1085株,尤其是成年植株数量剧减,由此导致年龄结构趋于幼年化,呈现正金字塔的假象;雌雄比从1∶10变为1∶5,这些现状表明德保苏铁的保护刻不容缓。     

Pollination Biology of Cycas panzhihuaensis L. Zhou et S.Y.Yang

The population of cone-bearing cycad, Cycas panzhihuaensis L. Zhou et S. Y. Yang, was male-biased. Although the number of male individuals was almost equal to that of female individuals, the clonal and cone production of male individuals was 1.31 and 2.21 times as much as that of female individuals respectively. The sequence of pollen shedding was from the base to the top of the cone and microsporophyll. The peak of daily pollen shedding occurred at noon and in the afternoon. The airborne pollen concentration decreased quickly within 2.55 m and finally maintained at a low level along with the distance from emitting male cone. The megastrobilus was transformed into a receptive state at the beginning of pollination due to the regular morphological changes of megasporophylls. Two kinds of ants and one kind of cockroaches were found to be active within the megas- trobili during pollination, albeit causing no tissue damage. The microstrobili gave off a strong odor of fennel that could dispel all the insects nearby. The sterile foliar structure of the megasporophyll was able to secret aqueous droplets at dawn which might function as transporting adhered pollen grains by dislodging and accumulating them on or near micropyles during the process of droplets falling. Resuits from field observation showed that pollination of C. panzhihuaensis might be accomplished by different pollinators. Pollen grains were firstly wind-transported from microstrobili to megastrobili and then insects and secreted droplets on the megasporophyll either directly or indirectly carried the pollen grains to ovules within a megastrobilus. However, insects might play as a subsidiary pollinator due to the preferential concentration of airborne pollen grains transported to the megasporophylls.     

秀叶苏铁——广西苏铁属一新种

本文发表了一苏铁属新种,即秀叶苏铁,对其特征进行了阐述。     

Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae)

Cycas debaoensis is one of the critically endangered cycad species endemic to China. In this study, we described the development of six microsatellite markers from the genome of C. debaoensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO) and two microsatellite markers derived from the database of expressed sequence tags (dbEST). Polymorphism of each locus was assessed in 60 adult individuals of the cycad. The average allele number of the microsatellites was 2.6 per locus, ranging from two to five. The observed and expected heterozygosities varied from 0.0833 to 0.7333 and from 0.0805 to 0.7188, respectively. Despite its rarity, only one locus (Y177) deviated from Hardy-Weinberg equilibrium due to the excessive homozygosity. The marker transferability of the eight primer pairs was tested on other four congeneric species that also occur in China.     

Isolation and characterization of microsatellite markers for Cycas hainanensis C. J. Chen (Cycadaceae)

Cycas hainanensis is an endangered cycad species endemic to Hainan Island, China. To enrich our scientific conservation for this species, we developed eight microsatellite markers using repetitive DNA enriched libraries. The number of alleles per microsatellite locus varied from 8 to 17. The expected (H _E ) and observed (H _O ) heterozygosities varied from 0.4754 to 0.8846 and from 0.3636 to 0.9600, respectively. These markers will be employed to determine whether the ex situ C. hainanensis individuals in Sourh China Botanical Garden capture a representative portion of genetic diversity of the wild populations.     

版纳省藤的组织培养

以版纳省藤的萌蘖芽为试材,从取材、材料的处理、外植体的诱导分化、芽的增殖、生根以及影响试管苗形成几个重要因素等方面探讨了版纳省藤组织培养和快速繁殖的技术和方法,获得了生长素与细胞分裂素对芽的诱导分化及芽的增殖最佳配比浓度。以改良MS为基本培养基,在增殖培养基中添加0.5mg·L-1NAA、0.5mg·L-1KT和0.1mg·L-1BA,产生的有效苗最多;在生根培养基中添加1.5～2.0mg·L-1IBA生长素有利于版纳省藤组培苗生根。     

珍珠矮

珍珠矮绘图：何顺清栽培及配文：钟树华,韦毅刚珍珠矮（ＣｙｍｂｉｄｕｍｎａｎｕｌｕｍＹ．Ｓ．ＷｕｅｔＳ．Ｃ．Ｃｈｅｎ）是我国著名兰科植物分类专家吴应祥、陈心启于１９９１年定名的新种,发表在《植物分类学报》第２９卷第６期上。模式标本是由余大鹏于１９８９年...     

杏黄兜兰

<正> 杏黄兜兰Paphiopedilum armeniacum S. C. Chen et F. Y. Liu是一种珍贵的兰科植物。分布于云南碧江县,生于岩壁上。是我国植物学家张敖罗于1979年7月采到,1982年经陈心启和刘芳媛定名的新种。属名Paphiopedilum 源自希腊字paphio(维纳斯的形容词)和pedilum(拖鞋),用以形容该属植物状若拖鞋的唇瓣。种加词armeniacum在拉丁语中为“杏树”,这里意为“杏黄色的”,该词另一个意思是“亚美尼亚(西亚的一个国名)的”。1992年2月杏黄兜兰在昆明市园林局主办的云南兰花展览会     

我国苏铁属一新种

我国苏铁属(苏铁种)一新种, 即念珠苏铁。     

The Correct Author Citation for Cycas hongheensis and C. parvula (Cycadaceae)

The names Cycas hongheensis and C. parvula were not validly published in 1994 when they were first proposed, but were validated in 1996 by a different author.     

台湾苏铁的分布及模式产地

对台湾苏铁Cycas taiwaniana的形态特征、分布和模式产地做了增补和修订。目前台湾苏铁仅分布在我国福建省的中南部,且仅有一株野生植株生长在平和县。台湾苏铁的模式株仍生长在漳州市陈元光庙前。     

兰属新种珍珠矮的组织培养与植株再生

1植物名称兰属植物新种珍珠矮(Cymbidium nanulum Y.S.Wu et S.C.Chen)。2材料类别九成熟蒴果内种子。3培养条件以1/2MS(大量、微量元素均减半)为基本培养基。(1)种子萌发培养基:1/2MS 6-BA 0.25 mg·L~(-1)(单位下同) NAA 0.5 活性炭2 g·L~(-1) 椰乳     

青城细辛的花器官发生

利用扫描电镜观察了青城细辛（Asarum splendens）的花器官发生过程。青城细辛的花器官为轮状结构,向心发生,依次为两轮3基数的花被原基,两轮6基数的雄蕊原基和一轮6基数的心皮原基。两轮花被原基互生,只有外轮（先发生的一轮）花被原基完全发育,而内轮（后发生的一轮）花被原基在发育过程中逐渐退化。两轮雄蕊原基为离心发生：位于内侧的一轮雄蕊原基先发生,每两个原基正对第一轮发生的花被原基,外侧的一轮雄蕊原基后发生,与内轮雄蕊原基互生。心皮与内侧的一轮雄蕊互生。     

海南岛苏铁属一新种

海南岛苏铁属（苏铁科）一新种,即三亚苏铁。