Responses of Baltic Sea Ice and Open-Water Natural Bacterial Communities to Salinity Change

To investigate the responses of Baltic Sea wintertime bacterial communities to changing salinity (5 to 26 practical salinity units), an experimental study was conducted. Bacterial communities of Baltic seawater and sea ice from a coastal site in southwest Finland were used in two batch culture experiments run for 17 or 18 days at 0°C. Bacterial abundance, cell volume, and leucine and thymidine incorporation were measured during the experiments. The bacterial community structure was assessed using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNA genes with sequencing of DGGE bands from initial communities and communities of day 10 or 13 of the experiment. The sea ice-derived bacterial community was metabolically more active than the open-water community at the start of the experiment. Ice-derived bacterial communities were able to adapt to salinity change with smaller effects on physiology and community structure, whereas in the open-water bacterial communities, the bacterial cell volume evolution, bacterial abundance, and community structure responses indicated the presence of salinity stress. The closest relatives for all eight partial 16S rRNA gene sequences obtained were either organisms found in polar sea ice and other cold habitats or those found in summertime Baltic seawater. All sequences except one were associated with the α- and γ-proteobacteria or the Cytophaga-Flavobacterium-Bacteroides group. The overall physiological and community structure responses were parallel in ice-derived and open-water bacterial assemblages, which points to a linkage between community structure and physiology. These results support previous assumptions of the role of salinity fluctuation as a major selective factor shaping the sea ice bacterial community structure.     

Zooplankton communities in the Southern Weddell Sea (Antartica)

Summary Standardized abundances of 40 frequently occurring macrozooplankton taxa collected by double-oblique bongo net hauls between the surface and 200 m depth were submitted to an agglomerative hierarchical cluster analysis, to characterize surface zooplankton communities in the southern and eastern Weddell Sea. The sensitivity of the analysis concerning reduction of the number of considered taxa (especially stages of species) was tested. Dominant taxa in the entire area over the whole period were copepods Metridia gerlachei and Calanoides acutus. Calanus propinquus, Appendicularia spp. and calyptopis-1 larvae of krill, Euphausia superba were abundant at 73°S/19°W. Euphausia crystallorophias and larval fish Pleuragramma antarcticum dominated at 77°S/40°W on the southern shelf. The cluster analysis revealed marked similarities between the southern shelf community and the January community further to the northeast. E. crystallorophias, and larval Pleuragramma antarcticum are thought to be transported in surface layers of the coastal current to the southwest. Oceanic species such as Thysanoessa sp. and E. superba are probably not transported in the same way. Possible mechanisms for the maintenance of the community structures are discussed. A simplified method of characterizing communities, based on analysis of euphausiids, is presented.     

Bacterial Activity in a Reservoir Determined by Autoradiography and its Relationships to Phyto- and Zooplankton

In the drinking water reservoir Římov (Southern Bohemia) bacterioplankton was studied during 1983. Special attention was given to the relationships between parameters of bacterial abundance, total and individual activity. Bacterial counts and biomass was assessed and autoradiographic determinations of the proportion of active bacteria incorporating thymidine (Th) and mixture of amino acids (AA) and total uptake rate of AA were made over a year in the surface layer and during summer stratification from the thermocline and 15 m depth. Specific activity of metabolically active bacteria (SAMAB) and specific activity per unit of biomass (SAUB) were negatively correlated with counts of metabolizing cells and with bacterial biomass, respectively. Total and individual heterotrophic activity and counts of bacteria coincided with the changes of phytoplankton biomass, whereas bacteria incorporating Th were more tightly correlated with primary production. The most significant relation of metabolically active bacteria was found to cladoceran biomass. Thus, this part of heterotrophic bacterial activity seems to be stimulated by leakage of dissolved organic matter from phytoplankton being disrupted and incompletely digested by cladocerans rather than from healthy photosynthetizing cells.     

Phytoplankton Spring Bloom Intensity Index for the Baltic Sea Estimated for the years 1992 to 2004

We introduce an index for estimating the annual phytoplankton spring bloom intensity in the Baltic Sea. It is based on chlorophyll a estimates calculated from automatically sampled fluorescence and chlorophyll a measurements on board cargo ships from 1992 to 2004. The intensity is described by an index including information on the chlorophyll a concentration and duration of the spring bloom period. In all of the years studied, the spring bloom was most intense in the Gulf of Finland. In the Gulf of Finland and the Northern Baltic Proper there was a slight tendency for the bloom to start earlier in the spring.     

Responses of Baltic Sea ice and open-water natural bacterial communities to salinity change

To investigate the responses of Baltic Sea wintertime bacterial communities to changing salinity (5 to 26 practical salinity units), an experimental study was conducted. Bacterial communities of Baltic seawater and sea ice from a coastal site in southwest Finland were used in two batch culture experiments run for 17 or 18 days at 0 degrees C. Bacterial abundance, cell volume, and leucine and thymidine incorporation were measured during the experiments. The bacterial community structure was assessed using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNA genes with sequencing of DGGE bands from initial communities and communities of day 10 or 13 of the experiment. The sea ice-derived bacterial community was metabolically more active than the open-water community at the start of the experiment. Ice-derived bacterial communities were able to adapt to salinity change with smaller effects on physiology and community structure, whereas in the open-water bacterial communities, the bacterial cell volume evolution, bacterial abundance, and community structure responses indicated the presence of salinity stress. The closest relatives for all eight partial 16S rRNA gene sequences obtained were either organisms found in polar sea ice and other cold habitats or those found in summertime Baltic seawater. All sequences except one were associated with the alpha- and gamma-proteobacteria or the Cytophaga-Flavobacterium-Bacteroides group. The overall physiological and community structure responses were parallel in ice-derived and open-water bacterial assemblages, which points to a linkage between community structure and physiology. These results support previous assumptions of the role of salinity fluctuation as a major selective factor shaping the sea ice bacterial community structure.     

Associations of Cyanobacterial Toxin,Nodularin, with Environmental Factors and Zooplankton in the Baltic Sea

Concentrations of a cyanobacterial toxin, nodularin, were measured in the Baltic Sea in 1998 and 1999. Statistical associations of nodularin concentrations with environmental factors were tested by multiple regression analysis. To reveal the toxin-producing organism, colonies of Aphanizomenon and filaments of Nodularia were picked and analyzed for peptide toxins. It was also investigated whether there was an association with zooplankton and Nodularia. All the measured seston samples contained nodularin, but other toxins were not detected by the HPLC analysis. In both years, the highest nodularin concentrations were found at the surface water layer. The nodularin concentrations were positively correlated with silicate concentrations in water. High concentrations of silica in surface water may indicate recent upwelling, which in turn renders surface water rich in nutrients. This upwelling is likely to intensify cyanobacterial growth and toxin production, which may explain this rather unexpected result. The picked Aphanizomenon colonies did not contain nodularin and the dissolved nodularin concentrations were below detection limit. Thus it was concluded that most of the nodularin was bound to Nodularia cells. The abundances of zooplankton (copepods, rotifers, and cladocerans) were unrelated to Nodularia, but were positively associated with Aphanizomenon.     

Planktonic ciliates of the Baltic Sea (a review)

The data on the ciliates species composition are given for the Baltic Sea, a brackish-water semi-closed water body where a considerable part of the planktonic fauna is presented by freshwater species. During the observation period, 789 species of ciliates were found, 160 of which are typical planktonic forms. The ecological characteristics of ciliates are given, along with an assessment of their role in the productivity of the Baltic Sea pelagic communities.     

Occurrence of Hemitaurichthys polylepis in the Coastal Waters of Southern Central Vietnam (South China Sea)

Journal of Ichthyology - The photographs of Hemitaurichthys polylepis in the natural environment of the coastal waters of Vietnam (Hon Noi) are given for the first time. The feeding of the...     

Analysis of fish parasitofauna in Vistula Lagoon (the Baltic Sea)

The characteristics of parasitofauna (81 species) of 15 fish species from Vistula Lagoon (the Baltic Sea) are given. Parasites with a direct developmental cycle dominate. Parasites with a complicated developmental cycle are represented mainly by species developed through zoobenthos. Some of the parasites testify to the deterioration of the ecological state of the lagoon. A zoogeographic analysis of fish parasitofauna has shown the presence of five faunistic complexes. In Vistula Lagoon freshwater species dominate, but marine species are also recorded. Species that appeared in the lagoon as a result of human commercial activity are also registered.     

Eye function of Mysidacea (Crustacea) in the northern Baltic Sea

Eye spectral sensitivity, [S(lambda)], was measured in seven northern Baltic mysid species using an electroretinogram technique. Their S(lambda) curves were compared with the spectral distribution of underwater light at their normal habitats. In the littoral species Neomysis integer, Praunus flexuosus and Praunus inermis, the S(lambda) maxima, [S(lambda)(max)], were in the wavelength-bands of 525-535, 505-515 and 520-530 nm respectively. The neoimmigrant species Hemimysis anomala had a S(lambda)(max) around 500 nm and high sensitivity at 393 nm, possibly indicating UV-sensitivity. S(lambda) of the pelagic species Mysis mixta and Mysis relicta sp. II was at about 505-520 nm. M. relicta sp. I from Pojoviken Bay and fresh water humic Lake P??j?rvi had S(lambda)(max) at approximately 550 nm and 570 nm respectively. This is in accordance with a similar long-wavelength shift in light transmittance of the respective waters. The eyes of the latter population were also damaged by strong light. The pontocaspian neoimmigrant H. anomala is clearly adapted to waters transmitting more blue light.     

Zooplankton community and hydrographical properties of the Neretva Channel (eastern Adriatic Sea)

Temporal and spatial variability of micro and mesozooplankton was studied in 1998 and 1999 at four stations in the Neretva Channel area influenced by the Neretva river and the open waters of the south Adriatic Sea. The area is orthophosphate limited, but an excessive accumulation of land derived nitrogen is prevented by phytoplankton uptake and the general circulation pattern. Microzooplankton was dominated by ciliates, with average abundances comparable to other Adriatic channel areas (122–543 ind. l⁻¹). Non-loricate ciliates (NLC) generally peaked in the warmer periods, but a winter increase was evident towards the inner part of the channel. Tintinnid abundances generally increased in autumn. A significant relationship with temperature was not recorded for either protozoan group. An inverse relationship between NLC and salinity might be indirectly caused by their preference for the food abundant surface layer. Mesozooplankton was dominated by copepods, with distinct summer maxima throughout the area and pronounced winter maxima of >10,000 ind. m⁻³ at the inner stations. The community was predominantly neritic but the open sea waters were important in structuring the mesozooplankton assemblage at all stations during the autumn–winter period. Although temperature regulated the seasonal dynamics of most metazoans and the species succession in the copepod community, small omnivorous copepods (Oncaea media complex, Oithona nana and Euterpina acutifrons) dominated regardless of the season. A trophic link between copepods and ciliates was evident in winter during low phytoplankton biomass.     

Drift-ice and under-ice water communities in the Gulf of Bothnia (Baltic Sea)

The algal, protozoan and metazoan communities within different drift-ice types (newly formed, pancake and rafted ice) and in under-ice water were studied in the Gulf of Bothnia in March 2006. In ice, diatoms together with unidentified flagellates dominated the algal biomass (226 ± 154 μg ww l⁻¹) and rotifers the metazoan and protozoan biomass (32 ± 25 μg ww l⁻¹). The under-ice water communities were dominated by flagellates and ciliates, which resulted in lower biomasses (97 ± 25 and 21 ± 14 μg ww l⁻¹, respectively). The under-ice water and newly formed ice separated from all other samples to their own cluster in hierarchical cluster analysis. The most important discriminating factors, according to discriminant analysis, were chlorophyll-a, phosphate and silicate. The under-ice water/newly formed ice cluster was characterized by high nutrient and low chlorophyll-a values, while the opposite held true for the ice cluster. Increasing trends in chlorophyll-a concentration and biomass were observed with increasing ice thickness. Within the thick ice columns (>40 cm), the highest chlorophyll-a concentrations (6.6–22.2 μg l⁻¹) were in the bottom layers indicating photoacclimation of the sympagic community. The ice algal biomass showed additional peaks in the centric diatom-dominated surface layers coinciding with the highest photosynthetic efficiencies [0.019–0.032 μg C (μg Chl-a ⁻¹ h⁻¹) (μE m⁻² s⁻¹)⁻¹] and maximum photosynthetic capacities [0.43-1.29 μg C (μg Chl-a ⁻¹ h⁻¹)]. Rafting and snow-ice formation, determined from thin sections and stable oxygen isotopic composition, strongly influenced the physical, chemical and biological properties of the ice. Snow-ice formation provided the surface layers with nutrients and possibly habitable space, which seemed to have favored centric diatoms in our study.     

Linking denitrifier community structure and prevalent biogeochemical parameters in the pelagial of the central Baltic Proper (Baltic Sea)

The oxic-anoxic interface of the water column of the Gotland Basin (central Baltic Sea) is characterised by defined biogeochemical gradients and is hypothesised to be a zone of pronounced denitrification. Our aim was to analyse the composition and distribution of pelagic denitrifying microorganisms in relation to the physico-chemical gradients in the water column. PCR-amplified nirS genes--coding for dissimilatory nitrite reductase--were analysed as functional markers by terminal restriction fragment length polymorphism and cloning. The overall nirS diversity was low, with the lowest levels found at the oxic-anoxic interface. Only a few terminal restriction fragments dominated the denitrifier communities throughout the water column, and these could be assigned to several new Baltic Sea clusters that were revealed by phylogenetic analysis. The novel clusters were separated in two groups corresponding to the oxygen concentrations within specific layers of the water column. Gradients of prevalent biogeochemical parameters (H(2)S, NH(4) (+), NO(3) (-) and O(2)) largely determined the composition of the nirS-type denitrifier communities within the water column of the Gotland Basin.     

Epidemiology of pseudobranchial tumours in Atlantic cod(Gadus morhua) from the North Sea and the Baltic Sea

Investigations during several cruises (1979–1981) showed that Atlantic cod with pseudobranchial tumours occurred in all areas investigated. Statistical analysis of data revealed that there was a marked prevalence of the condition in the centre of the German Bight, whereas a lower incidence of the condition was observed on the Dogger Bank, off the Dutch coast and in the western Baltic Sea. The higher prevalence of pseudobranchial tumours in the German Bight coincides with a dense population of cod and impacts of pollution.     

Ecology of mysid shrimps in the Bornholm Basin (central Baltic Sea)

Mysid shrimps are an important trophic link in the food web of the Baltic Sea. In 2002 and 2003 we investigated species composition, distribution, life cycle and prey in the Bornholm Basin (central Baltic). Three species and one genus were identified: Mysis mixta, Neomysis integer, Mesopodopsis slabberi and Praunus sp. The dominant M. mixta and N. integer occurred in the whole study area but were more abundant in the peripheral, more shallow regions. Size frequency distribution indicated one generation for M. mixta and probably two for N. integer. Main prey of all size classes in both species was the cladoceran Bosmina coregoni maritima during summer and autumn. In spring and winter also copepods of the species Temora longicornis and Pseudocalanus acuspes were important prey items. Detritus and phytoplankton cells were of minor importance and many individuals had empty guts. Mysid shrimps in the deep basin might be advected from more shallow areas, as the abundance of all species was much lower than in coastal regions. The deep Bornholm Basin does not seem to be a favourable habitat, probably due to the inaccessibility of the oxygen deficient bottom layer. Under current hydrographic conditions mysids are probably not important predators of zooplankton and thus not competitors of planktivorous fish in the Bornholm Basin.     

Histology of pseudobranchial tumours in Atlantic cod(Gadus morhua) from the North Sea and the Baltic Sea

Atlantic cod bearing pseudobranchial tumours were investigated from both North Sea and Baltic Sea areas. Light and electron microscopic studies revealed a tumorous structure previously described in cod from Atlantic and Pacific waters. Additionally, cysts, multinucleated cells and early stages of mitosis are described. These findings support the hypothesis that pseudobranchial tumours are instigated by a protozoan infection causing xenotumours associated with the pseudobranch organ. The cells contained in these lesions showed similarities to amoebae.