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1.
Sonja Kollers Bernd Rodemann Jie Ling Viktor Korzun Erhard Ebmeyer Odile Argillier Maike Hinze J?rg Plieske Dagmar Kulosa Martin W. Ganal Marion S. R?der 《PloS one》2013,8(2)
A total of 358 recent European winter wheat varieties plus 14 spring wheat varieties were evaluated for resistance to Fusarium head blight (FHB) caused by Fusarium graminearum and Fusarium culmorum in four separate environments. The FHB scores based on FHB incidence (Type I resistance)×FHB severity (Type II resistance) indicated a wide phenotypic variation of the varieties with BLUE (best linear unbiased estimation) values ranging from 0.07 to 33.67. Genotyping with 732 microsatellite markers resulted in 782 loci of which 620 were placed on the ITMI map. The resulting average marker distance of 6.8 cM allowed genome wide association mapping employing a mixed model. Though no clear population structure was discovered, a kinship matrix was used for stratification. A total of 794 significant (−log10(p)-value≥3.0) associations between SSR-loci and environment-specific FHB scores or BLUE values were detected, which included 323 SSR alleles. For FHB incidence and FHB severity a total of 861 and 877 individual marker-trait associations (MTA) were detected, respectively. Associations for both traits co-located with FHB score in most cases. Consistent associations detected in three or more environments were found on all chromosomes except chromosome 6B, and with the highest number of MTA on chromosome 5B. The dependence of the number of favourable and unfavourable alleles within a variety to the respective FHB scores indicated an additive effect of favourable and unfavourable alleles, i.e. genotypes with more favourable or less unfavourable alleles tended to show greater resistance to FHB. Assessment of a marker specific for the dwarfing gene Rht-D1 resulted in strong effects. The results provide a prerequisite for designing genome wide breeding strategies for FHB resistance. 相似文献
2.
Suspension cultures were established from embryogenic calli derived from cultured anthers of cv. Jinghua No.1 and mature embryos of cv. Youmangbai No. 7, respectively. After being isolated and cultured in WPMI, protoplasts began to form cell walls within 1 day post-isolation, followed by cell division observed between 2–3 days. A division frequency of 22.0% was estimated on the 7th day of culture, and 43.7% on the 14th day. During 10–15 days after the initiation of culture, a large number of cell aggregates emerged, with 0.5–0.8% of plating efficiency. Protoplast-derived calli grew up to lmm or more in diameter when cultured for 4 weeks, and eventually gave rise to green plants through embryogenesis and organogenesis after being transferred to differentiation media. Plant regeneration from protoplasts was already obtained from Jinghua No.l, and protoplast-derived calli from Youmangbai No.7; an experiment on organ differentiation for the latter is under way. A few factors affecting the protoplast cultures were also studied. 相似文献
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PEETERS KURT M. U.; GEUNS JAN M. C.; VAN LAERE ANDRE J. 《Journal of experimental botany》1993,44(11):1709-1715
The free polyamine content of flag leaves, peduncles, rachis,glumes, and grains of wheat (Triticum aestivum L., cv. Castell)plants, ripening under field conditions, has been investigatedduring three consecutive growing seasons. Putrescine was quantitativelythe most important of all polyamines detected in these organs.Concentrations were highest in the grains, glumes and flag leaves.No correlation was found between polyamine content and the onsetof senescence of flag leaves and other organs. Excised primaryleaves, however, showed a decrease in polyamine content in thedark and also in light/dark cycles, but in the latter case onlyafter an initial increase. Sink removal of otherwise intactwheat plants caused an accumulation of putrescine in flag leavesat the later stages of senescence, whereas removal of all otherleaves was without any significant effect. Putrescine was alsorecovered in phloem-exudate samples collected throughout theperiod of grain development. In both grains and glumes, peakconcentrations of polyamines were found early during seed development. Key words: Triticum aestivum, polyamines, ripening, senescence 相似文献
6.
Giovanni Laidò Daniela Marone Maria A. Russo Salvatore A. Colecchia Anna M. Mastrangelo Pasquale De Vita Roberto Papa 《PloS one》2014,9(4)
Association mapping is a powerful tool for the identification of quantitative trait loci through the exploitation of the differential decay of linkage disequilibrium (LD) between marker loci and genes of interest in natural and domesticated populations. Using a sample of 230 tetraploid wheat lines (Triticum turgidum ssp), which included naked and hulled accessions, we analysed the pattern of LD considering 26 simple sequence repeats and 970 mostly mapped diversity array technology loci. In addition, to validate the potential for association mapping in durum wheat, we evaluated the same genotypes for plant height, heading date, protein content, and thousand-kernel weight. Molecular and phenotypic data were used to: (i) investigate the genetic and phenotypic diversity; (ii) study the dynamics of LD across the durum wheat genome, by investigating the patterns of LD decay; and (iii) test the potential of our panel to identify marker–trait associations through the analysis of four quantitative traits of major agronomic importance. Moreover, we compared and validated the association mapping results with outlier detection analysis based on population divergence. Overall, in tetraploid wheat, the pattern of LD is extremely population dependent and is related to the domestication and breeding history of durum wheat. Comparing our data with several other studies in wheat, we confirm the position of many major genes and quantitative trait loci for the traits considered. Finally, the analysis of the selection signature represents a very useful complement to validate marker–trait associations. 相似文献
7.
QTL Mapping of Diffuse Reflectance Indices of Leaves in Hexaploid Bread Wheat (Triticum aestivum L.)
Chesnokov Yu. V. Kanash E. V. Mirskaya G. V. Kocherina N. V. Rusakov D. V. Lohwasser U. Börner A. 《Russian Journal of Plant Physiology》2019,66(1):77-86
Russian Journal of Plant Physiology - Quantitative trait loci (QTL) mapping of diffuse reflectance indices of laminas in bread wheat (Triticum aestivum L.) has been first performed under controlled... 相似文献
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Karagiannidou Th.; Eleftheriou E. P.; Tsekos I.; Galatis B.; Apostolakos P. 《Annals of botany》1995,76(1):23-30
Tubulin conformations other than microtubules in the meristematiccells of wheat roots grown in the presence of 2 mM colchicinesolution were investigated by immunofluorescence and electronmicroscopy. In the affected cells microtubules disappeared andwere replaced by tubulin fluorescent strands that occurred inthe cortical cytoplasm. With increasing time of exposure tocolchicine the tubulin strands became better organized and occurredalso in the subcortical cytoplasm and finally they were restrictedto the area around the nucleus. In prophase and preprophasecells thick strands occupied the cortical cytoplasmic zone wherein normal cells a preprophase microtubule band (PMB) was expectedto be assembled. In the colchicine-treated cells electron microscopy revealedan accumulation of paracrystalline aggregates, which initiallyoccurred along the cell wall and later deeper in the cytoplasm,in the perinuclear regions and the cytoplasmic invaginationsof the nucleus. In transverse planes the paracrystalline strandsappear to consist of hexagonal subunits in a 'honeycomb' arrangement,while in longitudinal and oblique sections they exhibit variableimages. Since their distribution coincides with that of thetubulin strands visualized by immunofluorescence, they are consideredto be the same structure. Therefore, the paracrystals consistof, or at least contain, tubulin. They are most likely to bepolymers of tubulin-colchicine complexes.Copyright 1995, 1999Academic Press Wheat roots, colchicine, immunofluorescence, electron microscopy, tubulin paracrystals, Triticum aestivum L 相似文献
10.
A Study of Floret Development in Wheat (Triticum aestivum L.) 总被引:1,自引:0,他引:1
Plants of wheat (Triticum aestlvum L.) cv. Aotea were grownat high or low nitrogen levels and in a natural photoperiodor continuous light. Starting 1721 days from the double-ridgestage, eight plants from each treatment were sampled every 3days until anthesis, and the two basal, the sixth, and the terminalspikelets were sectioned longitudinally. A developmental scorewas assigned to each floret and rates of development calculated.Continuous light hastened development but reduced the numberof spikelets per ear, while high nitrogen delayed developmentbut increased spikelet numbers. The number of florets initiatedin each spikelet varied within narrow limits, but grain settingdepended strongly on spikelet position and on treatment. Althoughflorets were initiated in acropetal succession, the rate ofdevelopment tended to increase up to floret 4 but then declinedmarkedly. As a result grain setting was confined to basal floretpositions, although the two basal spikelets developed so slowlythat they contributed relatively little to grain yield. Distalflorets degenerated almost simultaneously at or before ear emergence,but those in intermediate positions continued to develop untilafter fertilization in the lower florets. It is argued thatthe spikelet is an integrated system in which correlative mechanismsplay a part throughout the development of the florets. 相似文献
11.
The number and developmental stages of florets were determinedin each spikelet of the spike in the main shoots of spring wheat.Samples were taken frequently from plants grown in a phytotronand in a nitrogen application field-test. Ten stages of development,from floret initiation until anthesis, were recognized and described. Inter-spikelet variation in the total number of initiated floretswas rather small. However, the number of florets at advancedstages of development, as well as the number of grains, washighest in the central spikelets in which florets initiatedfirst. Floret initiation did not proceed beyond spike emergence,whereafter the distal florets and the spikelet apex degenerated.Grain-set was restricted to florets which had developed at leastto the stage of visible anther lobes at spike emergence. Thenumber of these florets was increased significantly by nitrogenapplication. Wheat, Triticum aestivum L., spikelet, floret, grain set, nitrogen 相似文献
12.
Greene FC 《Plant physiology》1981,68(3):778-783
Free and membrane-associated polysomes were isolated in approximately equal amounts from endosperm of wheat kernels harvested 20 days after anthesis. The presence of heparin in the homogenizing buffer minimized polysome degradation. Ribonucleic acid from the isolated polysomes, when translated in vitro in a wheat germ system, yielded products ranging in size from about 12,000 to about 80,000 daltons, including at least two polypeptides that co-migrated with seed extract proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis. The nature of the translation products of free and membrane-associated RNA are distinctly different, with membrane-associated RNA yielding a higher proportion of polypeptides in the size range of 30,000 to 37,000 daltons. Analysis of membrane-associated 3′-terminal polyadenylyl-containing RNA in vitro translation products, by solubility in 70% ethanol and by immunoprecipitation, indicates that the 33,000- to 37,000-dalton polypeptides contain gliadins, and the analysis provides evidence that these proteins are synthesized in association with membranous cell organelles. Gliadin polypeptides synthesized in vitro are larger than authentic gliadins and probably are precursors which, in vivo, undergo modification to yield the smaller final products. 相似文献
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Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves 总被引:14,自引:10,他引:4
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Protoplasts from 8- to 9-day-old wheat (Triticum aestivum L.) leaves were used to isolate organelles which were examined for their contents of peptide hydrolase enzymes and, in the case of vacuoles, other acid hydrolases. High yields of intact chloroplasts were obtained using both equilibrium density gradient centrifugation and velocity sedimentation centrifugation on sucrose-sorbitol gradients. Aminopeptidase activity was found to be distributed, in approximately equal proportions, between the chloroplasts and cytoplasm. Leucyltyrosine dipeptidase was mainly found in the cytoplasm, although about 27% was associated with the chloroplasts. Vacuoles shown to be free from Cellulysin contamination contained all of the protoplast carboxypeptidase and hemoglobin-degrading activities. The acid hydrolases, phosphodiesterase, acid phosphatase, α-mannosidase, and β-N-acetylglucosamidase were found in the vacuole to varying degrees, but no β-glucosidase was localized in the vacuole. 相似文献
14.
Parker G.D. Chalmers K.J. Rathjen A.J. Langridge P. 《Molecular breeding : new strategies in plant improvement》1999,5(6):561-568
A partial genetic linkage map constructed using 150 single seed descent (SSD) lines generated from a cross between the hexaploid wheat varieties Schomburgk and Yarralinka was used to identify loci controlling milling yield. Milling yield data were obtained using seed collected from field trials conducted at different sites over two seasons. The estimated broad-sense heritability of milling yield in this population was calculated as 0.48. In the preliminary analysis, two regions were identified on chromosomes 3A and 7D, which were significantly associated with milling yield and accounted for 22% and 19% of the genetic variation, respectively. Bulked segregant analysis in combination with AFLP identified other markers linked to these loci, as well as an additional region on chromosome 5A, which accounted for 19% of the genetic variation. The applicability of these markers as selection tools for breeding purposes is discussed. 相似文献
15.
G. D. Parker K. J. Chalmers A. J. Rathjen P. Langridge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):238-245
An RFLP map constructed using 150 single seed descent (SSD) lines from a cross between two hexaploid wheat varieties (‘Schomburgk’בYarralinka’)
was used to identify loci controlling flour colour. Flour colour data were obtained from field trials conducted over two seasons
at different sites. The estimated heritability of this trait was calculated as 0.67. Two regions identified in the preliminary
analysis on chromosomes 3A and 7A, accounted for 13% and 60% of the genetic variation respectively. A detailed analysis of
the major locus on 7A was conducted through fine mapping of AFLP markers identified using bulked segregant analysis (BSA).
Seven additional markers were identified by the BSA and mapped to the region of the 7A locus. The applicability of these markers
to identify wheat lines with enhanced flour colour is discussed.
Received: 30 September 1997 / Accepted: 4 February 1998 相似文献
16.
小麦抗倒性研究进展 总被引:9,自引:0,他引:9
姚金保 《植物遗传资源学报》2013,14(2):208-213
倒伏是严重影响小麦子粒产量和品质的一个重要因素。本文系统阐述了小麦茎秆形态和结构特性、茎秆化学成分与抗倒伏关系以及抗倒性的遗传和分子标记等方面的最新研究进展。株高、基部节间长度与抗倒性呈负相关;而基部节间粗度、秆壁厚、单位长度干重与抗倒性呈正相关。茎秆机械组织细胞层数、厚度,维管束数目、面积以及髓腔大小与抗倒性密切相关。茎秆化学成分中纤维素、木质素以及碳水化合物含量和硅、钾元素含量与抗倒性呈正相关。小麦抗倒性呈数量性状遗传特征,除受多对主基因控制外,可能还受微效修饰基因作用。采用分子标记技术已将抗倒性以及与抗倒性相关的茎秆形态性状进行了QTL定位。 相似文献
17.
Plants of Triticum aestivum L., cv. Gabo, were grown in a glasshousefor 4 weeks and then transferred to a controlled environmentwith 20±1 °C temperature and 16 h photoperiod. Theywere subjected to water deficit by withholding the water supplyduring various stages of floral development, including thoseimmediately before meiosis and all stages until just after anthesis. The proportion of apparently normal florets which produced grainwas reduced when water deficit occurred during and immediatelyafter meiosis in the generative tissues. The effect of thisreduced grain set on total grain yield was partially compensatedby an increase in the weight of the remaining grains. Cross-pollinationbetween stressed and well-watered plants showed that grain setwas reduced as a direct consequence of the induction of malesterility by water stress, whereas female fertility was unaffected.A large proportion of the anthers on water-stressed plants weresmall and shrivelled, did not dehisce normally and containedpollen which was devoid of normal cytoplasmic constituents andshowed no staining reaction with triphenyl tetrazolium chloride.This effect on male fertility was not a result of desiccationof the sporogenous tissue, but an indirect outcome of the decreasein water potential elsewhere in the plant. Water stress, Triticum aestivum L., wheat, pollen, sporogenesis, grain set, male sterility 相似文献
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利用79对多态性较高的SSR引物,对河北省1997-2007年间审定的冬小麦品种及国家小麦区试抗旱对照品种晋麦47和洛旱2号,共计87个冬小麦品种进行遗传多样性分析。79对SSR引物共检测出175个等位变异位点,每对引物可产生1~6条等位变异位点,平均2.215条。标记位点多态性信息含量(PIC)变幅为0.824~0.998,平均为0.941;有效等位基因数(Ne)变幅为1.644~20.333,平均4.708;香农指数(H’)变幅为0.148~1.102,平均为0.544,说明河北省冬小麦品种SSR遗传多样性较低。品种间遗传相似系数(GS)变幅为0.184~0.899,平均为0.418,其中河农826与石家庄8号间的遗传相似性最高,GS高达0.899,71-3与藁优9618间的遗传相似性最低,GS为0.184。不同育种单位培育的小麦品种平均遗传相似系数存在较大差异。UPGMA遗传相似性聚类表明,石家庄市小麦新品种新技术研究所培育的小麦品种与其他单位品种存在较大的遗传差异。 相似文献
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Mutations (Rht genes) blocking sensitivity to gibberellic acid (GA) were used to examine phytohormone mediated cell wall expansion in wheat (Triticum aestivum L.). Irreversible extensibility of immature leaf segments, as determined by stress/strain (instron) measurements, declined with Rht gene dose. Exogenous GA3 significantly increased wall extensibility in the nonmutant controls but had no effect on the near-isogenic GA-insensitive genotypes. Furthermore, ancymidol, an inhibitor of gibberellin biosynthesis, diminished wall extensibility in the nonmutant control. Extensibility of immature segments was highly correlated with mature leaf sheath length (R = +0.95). The results indicate that wall yielding properties of expanding wheat leaves are associated with leaf cell expansion potential and that GA is involved in the determination of those properties. 相似文献
20.
Plant regeneration was achieved from coleoptile tissue of wheat (Triticum aestivum L. cv. Kharachia-65). Coleoptiles (1.0
- 3.5 cm long) were excised from 2- to 5-d-old seedlings and cultured on Murashige and Skoog's (MS) medium supplemented with
2,4-dichlorophenoxyacetic acid (2,4-D - 0.5, 2.5, and 5.0 mg dm-3). Cream, friable callus was obtained after 6 weeks of inoculation. This callus was sub-cultured on MS medium supplemented
with 2,4-D (2.5 mg dm-3) and 5 % coconut water. After 6 weeks of sub-culturing white, cream or pale, friable, nodular callus was obtained. Plant
regeneration occurred when this callus was sub-cultured on MS medium supplemented with 0.2 mg dm-3 1-naphthalene acetic acid + 1.0 mg dm-3 6-benzylaminopurine. For rooting, regenerated shoots or plantlets were transferred on MS medium supplemented with 0.5 mg
dm-3 indole-3-acetic acid. Rooted plantlets were directly transferred into pots and grown under field conditions. Seed setting
invariably occurred in all plants.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献