On the Psychological Well-Being of Chimpanzees

It all started with a seemingly simple decision to re-evaluate and document the Phoenix Zoo's behavioral management protocol. The purpose of this project was to present proactive standards for the care and psychological well-being of our living collection, while meeting or exceeding the guidelines of the Animal Welfare Act (U. S. Department of Agriculture Animal and Plant Health and Inspection Service, Animal Care, 1999). Preparing the protocol was a catalyst to re-evaluate the zoo's philosophy and application of behavioral management. It suggested a restructuring of collection management and the rethinking of future goals and practices. Gradually, the process became more focused and organized. Behavioral enrichment, training, animal behavior issues, and exhibit architecture were embraced as essential components for providing quality of life. Staff from all levels worked side-by-side on assignments. Our way of thinking and working was changing.     

葡萄蔗糖转化酶基因家族的生物信息学及响应逆境胁迫分析

蔗糖转化酶(invertase, INV)在植物生长发育和抵御胁迫中发挥着重要作用。研究从葡萄基因组数据库中鉴定出19个蔗糖转化酶基因,对基因结构和编码蛋白质的理化性质进行生物信息学分析,并利用qRT-PCR技术分析基因在不同激素和非生物胁迫条件下的表达特征,为进一步探索葡萄INV基因家族参与葡萄逆境响应提供了一定的理论依据。结果表明,(1)该基因家族编码蛋白的氨基酸长度在150～766 aa之间,理论等电点介于4.43～9.1之间,亚细胞定位预测发现其主要在细胞质中表达,此外液泡和细胞壁也存在部分基因表达;(2)共线性结果显示VvCINV与其他5个物种复制频率较高;(3)保守基序分析表明VvCwINV包含了所有的保守基序,且Glyco＿32和Glyco＿hydro＿100是VvINV基因主要结构域;(4)组织特异性表达分析发现多数基因在葡萄生长发育进程中都有表达;(5)qRT-PCR分析结果显示,VvINV基因家族在叶片中对激素处理和非生物胁迫的响应出现上调,VvCINV1在50 mg/L GA₃和10%PEG处理后上调表达极显著,VvCINV4在盐胁迫、ABA...     

禾本科植物响应非生物胁迫的转录调控网络

分子和遗传研究表明,转录因子在响应非生物胁迫的基因表达调控中起着重要的作用,并且大部分转录因子在禾本科植物和拟南芥中是相同的.禾本科植物包括许多重要的农作物,对禾本科植物的转录因子进行研究,可增强重要农作物对非生物胁迫的耐受性.综述了禾本科植物响应非生物胁迫的转录调控网络,包括响应寒冷胁迫的DREB1/CBF调节子、响应脱水和高盐胁迫的DREB2调节子,ABA介导反应的ABRE及其伴侣元件、响应ABA的AREB/ABF调节子,响应脱水、高盐和寒冷胁迫的NAC调节子.