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ABSTRACT.
- 1 The mean supercooling points of first instar and adult Myzus persicae (Sulzer) maintained at 20°C and cooled at 1°C min?1 were ?26.6 and ?25.0°C respectively.
- 2 The LT50 (temperature) of the same age groups drawn from the same population and cooled at the same rate were ?8.1 and ?6.9°C, indicating extensive pre-freeze mortality in M.persicae under laboratory conditions.
- 3 Acclimation at 10 and 5°C did not affect supercooling but depressed the LT50 of both first instars and adult aphids.
- 4 Freezing of leaves during feeding did not increase mortality above that expected from the direct effects of low temperature.
- 5 The level of cold in different winters can be expressed in terms of the total number of frost days, and the frequency of abnormally cold days. Winter temperatures differ markedly in a vertical profile from the soil to the soil or grass surface, and then to the air (and foliage) above.
- 6 The time of the first record of M.persicae in suction trap samples is correlated with January and February temperatures except in the west of England and Wales. Further north December and January temperatures are relatively more important.
- 7 Winter temperatures and the resultant aphid mortality is a primary determinant of the timing of the spring migration.
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Glutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid 总被引:2,自引:0,他引:2
Glutathione S-transferases (GST) in insects play an important role in the detoxification of many substances including allelochemicals from plants. Induction of GST activity in Myzus persicae in response to secondary metabolites from Brassica plants was determined using different host plant species and confirmed using artificial diet with pure allelochemicals added. The 2,4-dinitro-1-iodobenzene (DNIB) was found to be a useful substrate for identifying particular GSTs in insects. GSTs from M. persicae were purified using different affinity chromatography columns and related kinetic parameters were calculated. GST isoenzymes were characterised using electrophoretic methods. Although SDS-PAGE results indicated similarity among the purified enzymes from each affinity column, biochemical studies indicated significant differences in kinetic parameters. Finally, the GST pattern of M. persicae was discussed in terms of insect adaptation to the presence of plant secondary substances such as the glucosinolates and the isothiocyanates, from Brassicaceae host plants. 相似文献
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大草蛉Chrysopa pallens(Rambur)是我国分布较广、捕食能力较强的天敌昆虫。为明确大草蛉对桃蚜Myzus percicae潜在的控制能力,在室内研究了大草蛉不同龄期幼虫和成虫对桃蚜的捕食作用,以及种内干扰对大草蛉捕食的影响。结果表明:25℃恒温条件下,大草蛉成虫和幼虫对桃蚜的捕食功能反应可拟合HollingⅡ圆盘方程,在24 h内大草蛉成虫、1龄幼虫、2龄幼虫和3龄幼虫对桃蚜理论最大捕食量分别为370.4头、21.5头、112.4头和217.4头。大草蛉成虫和幼虫对桃蚜的捕食量均随着猎物密度的增加而增加,当桃蚜数量增加到150头后,大草蛉成虫和幼虫捕食量的增加速度明显减缓。成虫及幼虫对桃蚜的搜寻效应随着猎物密度的增加而降低,内干扰作用随着大草蛉虫龄的增加而增强。试验结果表明大草蛉对桃蚜具有较强的控害能力,对研究桃蚜生物防治技术有重要参考价值。 相似文献
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The effect of temperature on the functional response of female adults of the two-spot ladybird, Adalia bipunctata L. (Coleoptera: Coccinellidae) was examined in petri dish arenas containing sweet pepper leaves infested with different densities of the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). The predator showed a type II functional response at three tested temperatures ranging from 19°C to 27°C. The theoretical maximum number of prey captured by the predator increased with temperature. Based on the random predator equation, the estimated attack rates ranged from 0.13 h?1 at 19°C to 0.35 h?1 at 27°C on a leaf area of 20–25 cm2. There was no significant difference between the attack rates of the predator at 23°C and 27°C. Handling time significantly decreased as temperature increased from 19°C (0.39 h) to 27°C (0.24 h). This study shows that A. bipunctata displays high predation rates on M. persicae for a wide range of temperatures, indicating its potential for augmentative releases against this aphid pest. The limitations of the predictions generated by functional response experiments are discussed. 相似文献
6.
桃蚜Myzus persicae(Sulzer)寄主广泛,是一种全球性的广食性害虫。为了探明不同桃树品种对桃蚜遗传分化的影响,我们采用微卫星分子标记技术对白油蟠桃,黄油蟠桃等7个桃树品种上的桃蚜进行遗传多样性研究。结果表明,7对微卫星引物在171个样本中检测到118个等位基因,平均每个位点有16.857个等位基因。不同品种桃树上桃蚜的遗传多样性较为丰富,表明种内存在着较大的遗传变异。种群间没有遗传分化或分化不明显,这可能是种群间存在着明显的基因交流而造成的,说明桃树品种间的差异对桃蚜的分化影响较小。 相似文献
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Adriana E. Alvarez ;Anahi M. Alberti D'Amato ;W. Fred Tjallingii ;Marcel Dicke ;Ben Vosman 《Insect Science》2014,21(6):727-740
Young leaves of the potato Solanum tuberosum L. cultivar Kardal contain resistance factors to the green peach aphid Myzuspersicae (Sulzer) (Hemiptera: Aphididae) and normal probing behavior is impeded. However, M. persicae can survive and reproduce on mature and senescent leaves of the cv. Kardal plant without problems. We compared the settling ofM. persicae on young and old leaves and analyzed the impact of aphids settling on the plant in terms of gene expression. Settling, as measured by aphid numbers staying on young or old leaves, showed that after 21 h significantly fewer aphids were found on the young leaves. At earlier time points there were no difference between young and old leaves, suggesting that the young leaf resistance factors are not located at the surface level but deeper in the tissue. Gene expression was measured in plants at 96 h postinfestation, which is at a late stage in the interaction and in compatible interactions this is long enough for host plant acceptance to occur. In old leaves of cv. Kardal (compatible interaction), M. persicae infestation elicited a higher number of differentially regulated genes than in young leaves. The plant response to aphid infestation included a larger number of genes induced than repressed, and the proportion of induced versus repressed genes was larger in young than in old leaves. Several genes changing expression seem to be involved in changing the metabolic state of the leaf from source to sink. 相似文献
8.
目的通过在不同温度条件下对霍乱弧菌生存能力的观察,探索大连市水域中霍乱弧菌在流行"间歇期"如何存活和越冬。方法在自然海水和湖水中分别接种本市分离出的埃尔托型霍乱弧菌流行株小川1b型(大E940014)和埃尔托型霍乱弧菌非流行株小川32l(大E940011),在1~2℃、5~6℃及10~12℃3个温度条件下检测霍乱弧菌生存能力。结果流行株(小川1b型)在1~2℃海水存活26 d,5~6℃存活28 d,10~12℃存活46 d。可见,10~12℃生存时间明显高于1~2℃或5~6℃(P〈0.05)。结论两类菌株的生存对环境温度均很敏感,温度越低死亡越快。在自然水体中,非流行株生存时间略长于流行株;而在除菌水体中两类菌存活能力基本一致。 相似文献
9.
W. H. PARRY 《The Annals of applied biology》1978,90(1):27-34
The supercooling distribution curves of Myzus persicae varied in response to feeding position, diet and age. Unfed instar I aphids supercooled to temperatures lower than –20 °C. Feeding aphids supercooled to higher temperatures, the earlier instars on subsidiary veins supercooling to lower temperatures than the late instars and adults on the main veins. Starvation decreased supercooling ability in some adult aphids. The supercooling ability of aphids feeding on sucrose solutions through a Parafilm membrane decreased in response to an increasing concentration, the position being reversed on 25% sucrose. Varying the amino acid concentration had no effect. The possible effects of this variable supercooling ability on the winter survival of the aphid are discussed. 相似文献
10.
温度和光周期对烟蚜性蚜的诱导 总被引:5,自引:1,他引:4
本文研究了温度和光周期对烟蚜性蚜诱导的影响,结果表明:郑州地区的红色和黄绿色烟蚜在10小时光照和15、20℃组合下可产生性雌和雄蚜,而在12、14和8小时及25℃条件下均未有性蚜产生。 相似文献
11.
Commercially-grown potato cultivars were assessed for antibiotic resistance to Myzus persicae in a culture room at 19°C with a 2°C range and a 16 h photoperiod. The resistant rankings obtained were consistent for the following aphid metrics: maturation time, teneral adult weight, embryo complement and the production of nymphs. Intrinsic rates of increase (rm) correlated with the above parameters of aphid growth and reproduction but this was not always the case for nymphal survival rate. The cultivars Ulster Tarn, Record and Maris Piper were relatively resistant to M. persicae and cultivars Desirée and King Edward were the most susceptible. When the aphid clone or the culture plants were changed there was no significant effect on the relative resistance of the potato cultivars under test. The relationship between the number of embryos in teneral adults and the production of nymphs by aphids was examined by grouping aphids in weight batches. There was a strong relationship between these two variables. 相似文献
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Abstract.
- 1 The survival of adult and first-instar Myzus persicae reared at 20°C and 10°C was investigated after brief (1 min) exposure in the absence of plant material to temperatures between −5°C and −25°C, and extended exposures on plants of 1–10 days at a constant 5°C, 3°C and −5°C and a 24 h cycling regime between 5°C (18 h) and −5°C (6 h).
- 2 Life stage, rearing temperature, period of exposure and temperature regime all had a significant effect on the ability of aphids to survive cold. The effects of life stage and rearing temperature were most noticeable following exposure to cycling temperatures and extended exposures at −5°C, and least apparent after 1 min exposures at lower sub-zero temperatures.
- 3 Mortality following exposure to temperatures cycling between −5°C and 5°C was greater than that at 3°C (the mean of the cycling temperatures) and less than at a constant −5°C, suggesting that when temperatures fluctuate by a few degrees around 0°C the minimum temperature may affect survival to a greater extent than the mean.
- 4 These results suggest that an overwintering population of acclimated M.persicae would persist without significant mortality after a period of 7–10 days with −5°C frosts each night.
14.
Resistance in Nicotiana species to the green peach aphid appears to result from the production of a toxic material produced by the aerial parts of the plant. Materials exuded from the leaf hairs which are transferred to glass micro-beakers are toxic to several species of aphids. The symptoms of such poisoning resemble those of nicotine poisoning, but resistance does not appear to be correlated with the amount of nicotine in the leaf.
The investigation reported in this paper is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. 相似文献
Zusammenfassung Nicotiana-Arten erzeugen einen Stoff, der für die grüne Pfirsichlaus, Myzus persicae und verschiedene andere Blattlausarten giftig ist. Dieser Giftstoff scheint von den Haaren der Pflanzen ausgeschieden zu werden. Er wird von den Blattläusen aufgenommen, während sie über die Pflanzen laufen, und vermutlich durch die Kutikula absorbiert. Wenn man diese Exsudate mit Kamelhaarpinseln von den Blättern resistenter Pflanzen in Mikrobecher überträgt, werden die Blattläuse verschiedener Art getötet, sobald sie in diese Becher gesetzt werden. Die von N. gossei, einer hoch resistenten Art, übertragene Substanz blieb in diesen Behältern wenigstens 28 Tage lang toxisch.Das erste Symptom der Vergiftigung ist eine Lähmung der Hinterbeine. Die beiden anderen Beinpaare werden kurz danach betroffen, sodass sich die Blattläuse nicht mehr aufrecht halten können. Die Endsymptome bestehen in ziemlich raschen, jedoch nicht heftigen Zuckungen der Beine und Antenne. Es scheint keinerlei Beziehung zwischen der Anfälligkeit der Nicotiana-Arten und der Nicotiana tabacum-Sorten durch die Grüne Pfirsichlaus und dem Nikotingehalt der Blätter zu bestehen. Reziproke interspezifische Pfropfungen zwischen resistenten und anfälligen Nicotiana-Arten verursachen keinerlei Veränderungen in der Resistenz von Unterlagen und Reissern.
The investigation reported in this paper is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. 相似文献
15.
云南烟蚜抗药性机制研究 总被引:1,自引:0,他引:1
通过比较云南烟蚜敏感品系和抗性品系的解毒酶(α-乙酸萘酯羧酸酯酶、β-乙酸萘酯羧酸酯酶)和靶标酶(乙酰胆碱酯酶)的活力,研究了烟蚜对有机磷、拟除虫菊酯和氨基甲酸酯类杀虫剂抗性的生化机制,并通过酯酶基因扩增检测和钠离子通道突变检测,研究了其抗性的分子机制。结果表明:α-乙酸萘酯羧酸酯酶活力增强是烟蚜对有机磷类、氨基甲酸酯类杀虫剂及拟除虫菊酯类杀虫剂的抗性机制之一;乙酰胆碱酯酶在烟蚜对有机磷杀虫剂抗性中起重要作用;3个抗性品系烟蚜均没有发生酯酶基因扩增,抗拟除虫菊酯品系烟蚜发生了钠离子通道突变。 相似文献
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J SYLLER 《The Annals of applied biology》1994,125(1):141-145
The concentration of potato leafroll luteovirus (PLRV) did not differ in potato plants with secondary infections grown at 15°C or 27°C. Detached leaves of plants grown at 15°C or 27°C were used as sources of PLRV for peach-potato aphids (Myzus persicae Sulz.) both at 15°C and 27°C. At comparable temperature during virus acquisition, aphids which fed on leaves of plants kept previously at 15°C contained more viral antigen detected by ELISA than aphids which fed on leaves of plants grown at 27°C. The aphids which acquired PLRV at 27°C contained evidently more viral antigen than those which acquired PLRV at 15°C. The greatest amount of PLRV was found in the aphids which acquired the virus at 27°C from the leaves of plants kept at 15°C. The ability of M. persicae to transmit PLRV to Physalis ftoridana Rydb. generally decreased with decrease in the amount of PLRV in vectors. 相似文献
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Myzus persicae transferred to rape produced offspring with higher survival, larger weight and faster development than aphids transferred to beet, irrespective of whether they had been reared on rape or beet. Aphids transferred from rape to beet produced fewer offspring than those reared continuously on rape. On beet, rape-reared adults produced progeny with lower survival, slower development, smaller adult size and lower fecundity than the progeny of beet-reared individuals, but there were no differences in these characteristics associated with host plant after one generation. Aphids from rape were less than half as efficient in transmitting beet yellows virus as aphids reared continuously on beet but after one subsequent generation on beet this difference had disappeared completely. These results are discussed in relation to field conditions. 相似文献
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七星瓢虫成虫对烟蚜的捕食作用 总被引:22,自引:0,他引:22
对七星瓢虫CoccinellaseptempunctataL .成虫对烟蚜Myzuspersicae的捕食作用进行了定量研究。七星瓢虫成虫对烟蚜的功能反应属HollingⅡ型反应 ,拟合的圆盘方程为Na =1 .1 5 76Nt ( 1 +0 .0 0 3 48Nt) ,χ2 检验表明圆盘方程理论值与实测值相符。捕食选择试验表明在烟蚜、烟青虫Helicoverpaassaut(Guenee)卵和 1龄幼虫 3种猎物中 ,七星瓢虫成虫最喜好烟蚜。七星瓢虫成虫对自身密度的功能反应用Watt模型拟合为A =2 90 .0 3P-0 .7584,其捕食作用率与个体间相互干扰作用的关系用Hassell模型拟合为E =0 .8783 7P-0 .1 0 0 94。文中进一步就烟田中七星瓢虫的保护利用进行了讨论。 相似文献
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《昆虫知识》2018,(6)
【目的】探究黄色及其配色对烟蚜Myzuspersicae行为的影响,有助于烟蚜绿色防控技术的优化。为田间黄板诱蚜的应用技术研发提供理论指导。【方法】利用电脑软件设置8种不同RGB黄色以及不同颜色的双色图卡,测定烟蚜对色板的趋性反应。【结果】RGB数值为215∶255∶6的3号黄色对烟蚜的引诱作用显著高于其他黄色,其对烟蚜的平均引诱量为8.5头/块。在3号黄色的基础上配置另一种颜色,发现在面积比2∶1的比例下,相对于3号色板只有3-黑色板降低了诱虫量。在1∶1的比例下,3号黄色比所有双色板对烟蚜的引诱作用都强,说明增加配色的比例会降低黄板的引诱力。在1∶1的比例下不同双色板的引诱力也存在着显著差异,双色之间存在着互作现象,这表明不同配色会影响色板对蚜虫的引诱作用。【结论】3号黄色色板对烟蚜的引诱效果最好。 相似文献