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The Role of Epigenetic Regulation and Pluripotency‐Related MicroRNAs in Differentiation of Pancreatic Stem Cells to Beta Cells 下载免费PDF全文
Ediz Coskun Merve Ercin Selda Gezginci‐Oktayoglu 《Journal of cellular biochemistry》2018,119(1):455-467
In this study, we aimed to research the effects of class‐I HDACs and glucose on differentiation of pancreatic islet derived mesenchymal stem cells (PI‐MSCs) to beta cells. Beta cell differentiation determined by flow cytometric analysis and gene expression levels of PDX1, PAX4, PAX6, NKX6.1, NGN3, INS2, and GLUT2. As a result the valproic acid, is an inhibitor of class‐I HDACs, caused the highest beta cell differentiation in PI‐MSCs. However, the cells in this group were at early stages of differentiation. Glucose co‐administration to this group carried the differentiation to higher levels, but these newly formed beta cells were not functional. Moreover, reduction in the levels of pluripotency factors that Oct3/4, c‐Myc, and Nanog were parallel to beta cell differentiation. Also, the levels of HDAC1 and acetylated H3/H4 were increased and methylated H3 was decreased by VPA treatment. In addition, we have detected over expression in genes of miR‐18a‐5p, miR‐19b‐5p, miR‐30d‐3p, miR‐124, miR‐146a‐5p, miR‐184, miR‐335, and miR‐433‐5p in parallel to beta cell differentiation. As the conclusion, this study is important for understanding the epigenetic mechanism that controls the beta cell differentation and it suggests new molecules that can be used for diagnosis, and treatment of diabetes. J. Cell. Biochem. 119: 455–467, 2018. © 2017 Wiley Periodicals, Inc. 相似文献
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Rosa M. Guzzo PhD Jason Gibson Ren‐He Xu Francis Y. Lee Hicham Drissi PhD 《Journal of cellular biochemistry》2013,114(2):480-490
Induced pluripotent stem cells (iPSC) hold tremendous potential for personalized cell‐based repair strategies to treat musculoskeletal disorders. To establish human iPSCs as a potential source of viable chondroprogenitors for articular cartilage repair, we assessed the in vitro chondrogenic potential of the pluripotent population versus an iPSC‐derived mesenchymal‐like progenitor population. We found the direct plating of undifferentiated iPSCs into high‐density micromass cultures in the presence of BMP‐2 promoted chondrogenic differentiation, however these conditions resulted in a mixed population of cells resembling the phenotype of articular cartilage, transient cartilage, and fibrocartilage. The progenitor cells derived from human iPSCs exhibited immunophenotypic features of mesenchymal stem cells (MSCs) and developed along multiple mesenchymal lineages, including osteoblasts, adipocytes, and chondrocytes in vitro. The data indicate the derivation of a mesenchymal stem cell population from human iPSCs is necessary to limit culture heterogeneity as well as chondrocyte maturation in the differentiated progeny. Moreover, as compared to pellet culture differentiation, BMP‐2 treatment of iPSC‐derived MSC‐like (iPSC–MSC) micromass cultures resulted in a phenotype more typical of articular chondrocytes, characterized by the enrichment of cartilage‐specific type II collagen (Col2a1), decreased expression of type I collagen (Col1a1) as well as lack of chondrocyte hypertrophy. These studies represent a first step toward identifying the most suitable iPSC progeny for developing cell‐based approaches to repair joint cartilage damage. J. Cell. Biochem. 114: 480–490, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
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Effects Of Hypoxia in Long‐Term In Vitro Expansion of Human Bone Marrow Derived Mesenchymal Stem Cells 下载免费PDF全文
Annelise Pezzi Bruna Amorin Álvaro Laureano Vanessa Valim Alice Dahmer Bruna Zambonato Filipe Sehn Ianaê Wilke Lia Bruschi Maria Aparecida Lima da Silva Eduardo Filippi‐Chiela Lucia Silla 《Journal of cellular biochemistry》2017,118(10):3072-3079
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Microenvironmental Interaction Between Hypoxia and Endothelial Cells Controls the Migration Ability of Placenta‐Derived Mesenchymal Stem Cells via α4 Integrin and Rho Signaling 下载免费PDF全文
Jong Ho Choi Seung Mook Lim Yong In Yoo Jieun Jung Jong‐Won Park Gi Jin Kim 《Journal of cellular biochemistry》2016,117(5):1145-1157
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Inhibitory Effect of Hsa‐miR‐590‐5p on Cardiosphere‐derived Stem Cells Differentiation Through Downregulation of TGFB Signaling 下载免费PDF全文
Samaneh Ekhteraei‐Tousi Bahram Mohammad‐Soltani Majid Sadeghizadeh Seyed Javad Mowla Sepideh Parsi Masoud Soleimani 《Journal of cellular biochemistry》2015,116(1):179-191
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