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1.
’Forrest’ resistance to the soybean cyst nematode is bigenic: saturation mapping of the Rhg1and Rhg4 loci 总被引:1,自引:0,他引:1
K. Meksem P. Pantazopoulos V.N. Njiti L.D. Hyten P. R. Arelli D. A. Lightfoot 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(5):710-717
Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv ’Forrest’ is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived
from a cross of Forrest×Essex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP
primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg1 and Rhg4. The two QTLs involved in resistance to SCN were strongly associated with the AFLP marker EATGMCGA87 (P=0.0001, R2=24.5%) on linkage group G, and the AFLP marker ECCGMAAC405 (P=0.0001, R2 =26.2%) on linkage group A2. Two- way analysis of variance showed epistasic interaction (P=0.0001, R2 =16%) between the two loci controlling SCN resistance in Essex×Forrest recombinant inbred lines. Considering the two loci
as qualitative genes and the resistance as female index FI <5%, jointly the two loci explained over 98% of the resistance.
The locations of the two QTLs were confirmed in the NILs populations. Therefore SCN resistance in Forrest×Essex is bigenic.
High-efficiency marker-assisted selection can be performed using the markers to develop cultivars with stable resistance to
SCN.
Received: 5 November 2000 / Accepted: 23 January 2001 相似文献
2.
G. M. Timmerman T. J. Frew N. F. Weeden A. L. Miller D. S. Goulden 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(8):1050-1055
Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR). 相似文献
3.
Mapping and confirmation of a new sudden death syndrome resistance QTL on linkage group D2 from the soybean genotypes PI 567374 and ‘Ripley’ 总被引:1,自引:0,他引:1
Austeclinio L. de Farias Neto Rizwan Hashmi Michael Schmidt Shawn R. Carlson Glen L. Hartman Shuxian Li Randall L. Nelson Brian W. Diers 《Molecular breeding : new strategies in plant improvement》2007,20(1):53-62
The use of resistant cultivars is the most effective method for controlling sudden death syndrome (SDS), caused by Fusarium solani f. sp. glycines (FSG) (syn. Fusarium virguliforme Akoi, O’Donnell, Homma and Lattanzi), in soybean [Glycine max (L.) Merr.]. Previous research has led to the identification of soybean genotypes with partial resistance to SDS and quantitative
trait loci (QTL) controlling this resistance. The objective of our study was to map QTL conferring SDS resistance in populations
developed from the crosses Ripley × Spencer (R×S-1) and PI 567374 × Omaha (P×O-1). Both Ripley and PI 567374 have partial
resistance to SDS and Spencer and Omaha are susceptible. The R×S-1 population was evaluated for SDS resistance in three field
environments and the P×O-1 population was greenhouse evaluated. Three SDS resistance QTL were mapped in the R×S-1 population
and two in the P×O-1 population. One resistance QTL was mapped to the same location on linkage group (LG) D2 in both backgrounds.
This QTL was then tested in a population of F2 plants developed through one backcross (BC1F2) in the PI 567374 source and in a population of F8 plants derived from a heterozygous F5 plant in the Ripley source. The LG D2 QTL was also significant in confirmation populations in both resistant backgrounds.
Since none of the SDS resistance QTL identified in the R×S-1 or P×O-1 populations mapped to previously reported SDS resistance
regions, these new QTL should be useful sources of SDS resistance for soybean breeders. 相似文献
4.
Wang C Ulloa M Roberts PA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):770-777
Host-plant resistance is the most economic and effective strategy for root-knot nematode (RKN) Meloidogyne incognita control in cotton (Gossypium hirsutum L.). Molecular markers linked to resistance are important for incorporating resistance genes into elite cultivars. To screen
for microsatellite markers (SSR) closely linked to RKN resistance in G. hirsutum cv. Acala NemX, F1, F2, BC1F1, and F2:7 recombinant inbred lines (RILs) from intraspecific crosses and an F2 from an interspecific cross with G. barbadense cv. Pima S-7 were used. Screening of 284 SSR markers, which cover all the known identified chromosomes and most linkage groups
of cotton, was performed by bulked segregant analysis, revealing informative SSRs. The informative SSRs were then mapped on
the above populations. One co-dominant SSR marker CIR316 was identified tightly linked to a major resistance gene (designated
as rkn1), producing amplified DNA fragments of approximately 221 bp (CIR316a) and 210 bp (CIR316c) in Acala NemX and susceptible
Acala SJ-2, respectively. The linkage between CIR316a marker and resistance gene rkn1 in Acala NemX had an estimated distance of 2.1–3.3 cM depending on the population used. Additional markers, including BNL1231
with loose linkage to rkn1 (map distance 25.1–27.4 cM), BNL1066, and CIR003 allowed the rkn1 gene to be mapped to cotton linkage group A03. This is the first report in cotton with a closely linked major gene locus
determining nematode resistance, and informative SSRs may be used for marker-assisted selection. 相似文献
5.
A genetic linkage map of tef [Eragrostis tef (Zucc.) Trotter] based on amplified fragment length polymorphism 总被引:2,自引:0,他引:2
G. Bai H. Tefera M. Ayele H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):599-604
A genetic linkage map of tef was constructed with amplified fragment length polymorphism (AFLP) markers using F5 recombinant inbred lines (RILs) derived by single seed descent from the intraspecific cross of ’Kaye Murri’×’Fesho’. A total
of 192 EcoRI/MseI primer combinations were screened for parental polymorphism. Around three polymorphic fragments per primer combination were
detected, indicating a low polymorphism level in tef. Fifty primer combinations were selected to assay the mapping population,
and 226 loci segregated among 85 F5 RILs. Most AFLP loci behaved as dominant markers (presence or absence of a band), but about 15% of the loci were codominant.
Significant deviations from the expected Mendelian segregation ratio were observed for 26 loci. The genetic linkage map comprised
211 markers assembled into 25 linkage groups and covered 2,149 cM of genome. AFLP is an efficient marker system for mapping
plant species with low polymorphism such as tef. This is the first genetic linkage map constructed for tef. It will facilitate
the mapping of genes controlling agronomically important traits and cultivar improvement in tef.
Received: 27 April 1998 / Accepted: 4 January 1999 相似文献
6.
Weng Y Li W Devkota RN Rudd JC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(3):462-469
A new source of greenbug (Schizaphis graminum Rondani) resistance derived from Aegilops tauschii (Coss.) Schmal was identified in W7984, a synthetic hexaploid wheat line and one parent of the International Triticeae Mapping Initiative (ITMI) mapping population. Segregation analysis of responses to greenbug feeding in a set of recombinant inbred lines (RILs) identified a single, dominant gene governing the greenbug resistance in W7984, which was placed in chromosome arm 7DL by linkage analysis with molecular markers in the ITMI population. Allelism tests based on the segregation of responses to greenbug feeding in F2 and testcross plants revealed that the greenbug resistance in W7984 and Largo, another synthetic line carrying the greenbug resistance gene Gb3, was controlled by different but linked loci. Using the ITMI reference map and a target mapping strategy, we have constructed a microsatellite map of Gb3 in a mapping population of 130 F7 RILs from Largo × TAM 107 and identified one marker (Xwmc634) co-segregating with Gb3 and four markers (Xbarc76, Xgwm037, Xgwm428 and Xwmc824) closely linked with Gb3. Deletion mapping of selected microsatellite markers flanking the Gb3 locus placed this resistance gene into the distal 18% region of 7DL. Comparative mapping in the ITMI and Largo × TAM 107 populations using the same set of microsatellite markers provided further evidence that greenbug resistance in W7984 and Largo is conditioned by two different loci. We suggest that the greenbug resistance gene in W7984 be designated Gb7. The microsatellite map of Gb3 constructed from this study should be a valuable tool for marker-assisted selection of Gb3-conferred greenbug resistance in wheat breeding. 相似文献
7.
Mapping of SMV resistance gene Rsc-7 by SSR markers in soybean 总被引:8,自引:0,他引:8
Soybean mosaic virus (SMV) is one of the most prevalent pathogens that limit soybean production. In this study, segregation
ratios of resistant plants to susceptible plants in P1, P2, F1, F2 populations of Kefeng No. 1 (P1)×Nannong 1138-2 (P2) and derived RIL populations, were used to study the inheritance of resistance to the SMV strain SC-7. Populations Kefeng
No. 1 and F1 were found to be completely resistant to this SMV strain while Nannong 1138-2 was susceptible to it. The F2 and RIL populations segregated to fit a ratio of 3:1 and 1:1for resistant plants to susceptible ones, respectively. These
results indicated that a single dominant gene, designated as Rsc-7, controlled resistance to the SMV strain SC-7 in Kefeng No.1. SSR markers were used to analyze the RIL population and MAPMAKER/EXP
3.0b was employed to establish linkage between markers and this resistance gene. Combining the data of SSRs and resistance
identification, a soybean genetic map was constructed. This map, covering 2625.9 cM of the genome, converged into 24 linkage
groups, consisted of 221 SSR markers and the resistance gene Rsc-7. The Rsc-7 gene was mapped to the molecular linkage group G8-D1b+W. SSR markers Satt266, Satt634, Satt558, Satt157, and Satt698 were
found linked to Rsc-7 with distances of 43.7, 18.1, 26.6, 36.4 and 37.9 cM, respectively. 相似文献
8.
Sompong Chankaew Prakit Somta Worawit Sorajjapinun Peerasak Srinives 《Molecular breeding : new strategies in plant improvement》2011,28(2):255-264
Cercospora leaf spot (CLS) caused by the fungus Cercospora canescens Illis & Martin is a serious disease in mungbean (Vigna radiata (L.) Wilczek), and disease can reduce seed yield by up to 50%. We report here for the first time quantitative trait loci
(QTL) mapping for CLS resistance in mungbean. The QTL analysis was conducted using F2 (KPS1 × V4718) and BC1F1 [(KPS1 × V4718) × KPS1] populations developed from crosses between the CLS-resistant mungbean V4718 and CLS-susceptible cultivar
Kamphaeng Saen 1 (KPS1). CLS resistance in F2 populations was evaluated under field conditions during the wet seasons of 2008 and 2009, and resistance in BC1F1 was evaluated under field conditions during the wet season in 2008. Seven hundred and fifty-three simple sequence repeat
(SSR) markers from various legumes were used to assess polymorphism between KPS1 and V4718. Subsequently, 69 polymorphic markers
were analyzed in the F2 and BC1F1 populations. The results of segregation analysis indicated that resistance to CLS is controlled by a single dominant gene,
while composite interval mapping consistently identified one major QTL (qCLS) for CLS resistance on linkage group 3 in both F2 and BC1F1 populations. qCLS was located between markers CEDG117 and VR393, and accounted for 65.5–80.53% of the disease score variation depending on
seasons and populations. An allele from V4718 increased the resistance. The SSR markers flanking qCLS will facilitate transferral of the CLS resistance allele from V4718 into elite mungbean cultivars. 相似文献
9.
Dagang Wang Ying Ma Yongqing Yang Ning Liu Chunyan Li Yingpei Song Haijian Zhi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(3):555-565
Soybean mosaic virus (SMV) in soybean [Glycine max (L.) Merr.] is a destructive foliar disease in soybean-producing countries worldwide. In this study, F2, F2:3, and F7:11 recombinant inbred lines populations derived from Kefeng No.1 × Nannong 1138-2 were used to study inheritance and linkage
mapping of the SMV strain SC8 resistance gene in Kefeng No.1. Results indicated that a single dominant gene (designated R
SC8
) controls resistance, which is located on chromosome 2 (MLG D1b). A mixed segregating population was developed by selfing
two heterozygous plants (RHL153-1 and RHL153-2) at four markers adjacent to the locus and used in fine mapping R
SC8
. In addition, two genomic-simple sequence repeats (SSR) markers BARCSOYSSR_02_0610 and BARCSOYSSR_02_0616 were identified
that flank the two sides of R
SC8
. Sequence analysis of the soybean genome indicated that the interval between the two genomic-SSR markers is 200 kb. QRT-PCR
analysis of the candidate genes determined that five genes (Glyma02g13310, 13320, 13400, 13460, and 13470) are likely involved
in soybean SMV resistance. These results will have utility in cloning, transferring, and pyramiding of the R
SC8
through marker-assisted selection in soybean breeding programs. 相似文献
10.
Yang DE Jin DM Wang B Zhang DS Nguyen HT Zhang CL Chen SJ 《Molecular genetics and genomics : MGG》2005,274(3):229-234
The maize inbred lines 1145 (resistant) and Y331 (susceptible), and the F1, F2 and BC1F1 populations derived from them were inoculated with the pathogen Pythium inflatum Matthews, which causes stalk rot in Zea mays. Field data revealed that the ratio of resistant to susceptible plants was 3:1 in the F2 population, and 1:1 in the BC1F1population, indicating that the resistance to P. inflatum Matthews was controlled by a single dominant gene in the 1145×Y331 cross. The gene that confers resistance to P. inflatum Matthews was designated Rpi1 for resistance to P. inflatum) according to the standard nomenclature for plant disease resistance genes. Fifty SSR markers from 10 chromosomes were first
screened in the F2 population to find markers linked to the Rpi1 gene. The results indicated that umc1702 and mmc0371 were both linked to Rpi1, placing the resistance gene on chromosome 4. RAPD (randomly amplified polymorphic DNA) markers were then tested in the F2population using bulked segregant analysis (BSA). Four RAPD products were found to show linkage to the Rpi1 gene. Then 27 SSR markers and 8 RFLP markers in the region encompassing Rpi1 were used for fine-scale mapping of the resistance gene. Two SSR markers and four RFLP markers were linked to the Rpi1 gene. Finally, the Rpi1 gene was mapped between the SSR markers bnlg1937 and agrr286 on chromosome 4, 1.6 cM away from the former and 4.1 cM distant
from the latter. This is the first time that a dominant gene for resistance to maize stalk rot caused by P. inflatum Matthews has been mapped with molecular marker techniques. 相似文献
11.
Inheritance of resistance to southern corn rust in tropical-by-corn-belt maize populations 总被引:9,自引:0,他引:9
J. B. Holland D. V. Uhr D. Jeffers M. M. Goodman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(2):232-241
The inheritance of resistance to southern rust (caused by Puccinia polysora Underw.) was investigated in two F2:3 populations derived from crossing two temperate-adapted, 100% tropical maize (Zea mays L.) inbred lines (1416-1 and 1497-2) to a susceptible Corn Belt Dent hybrid, B73Ht×Mo17Ht. The inbred lines possess high levels of resistance to southern rust and may be unique sources of resistance genes. Heritability
for resistance was estimated as 30% and 50% in the two populations from regression of F2:3 family mean scores on F2 parent scores, and as 65% and 75% from variances among F2:3 families on a single-plot basis. RFLP loci on three chromosomal regions previously known to possess genes for resistance
to either southern rust or common rust (P. sorghi Schw.) were used to localize genes affecting resistance to southern rust in selected genotypes of both populations, and to
estimate their genetic effects. A single locus on 10S, bnl3.04, was associated with 82–83% of the variation among field resistance scores of selected F2:3 families in the two populations. Loci on chromosomes 3 (umc26) and 4 (umc31) were significantly associated with resistance in the 1497-2 population, each accounting for 13–15% of the phenotypic variation
for F2:3 field scores. Multiple-marker locus models, including loci from chromosomes 3, 4, and 10 and their epistatic interactions,
accounted for 96–99% of the variation in F2:3 field scores. Similar results were obtained for resistance measured by counting pustules on juvenile plants in the greenhouse.
An attempt was made to determine if the major gene for resistance from 1416-1 was allelic to Rpp9, which is also located on 10S. Testcross families from the cross (1416-1×B37Rpp9)×B14AHt were evaluated for resistance to southern rust in Mexico. Neither source of resistance was completely effective in this environment,
preventing determination of allelism of the two genes; however, both sources of resistance had better partial resistance to
southern rust than did B14AHt.
Received: 6 May 1997/Accepted: 19 September 1997 相似文献
12.
S. Fondevilla Z. Satovic D. Rubiales M. T. Moreno A. M. Torres 《Molecular breeding : new strategies in plant improvement》2008,21(4):439-454
Aschochyta blight, caused by Mycosphaerella pinodes, is one of the most economically serious pea pathogens, particularly in winter sowings. The wild Pisum sativum subsp. syriacum accession P665 shows good levels of resistance to this pathogen. Knowledge of the genetic factors controlling resistance
to M. pinodes in this wild accession would facilitate gene transfer to pea cultivars; however, previous studies mapping resistance to M. pinodes in pea have never included this wild species. The objective of this study was to identify quantitative trait loci (QTL) controlling
resistance to M. pinodes in P. sativum subsp. syriacum and to compare these with QTLs previously described for the same trait in P. sativum. A population formed by 111 F6:7 recombinant inbred lines derived from a cross between accession P665 and a susceptible pea cultivar (Messire) was analysed
using morphological, isozyme, RAPD, STS and EST markers. The map developed covered 1214 cM and contained 246 markers distributed
in nine linkage groups, of which seven could be assigned to pea chromosomes. Six QTLs associated with resistance to M. pinodes were detected in linkage groups II, III, IV and V, which collectively explained between 31 and 75% of the phenotypic variation
depending of the trait. While QTLs MpIII.1 and MpIII.2 were detected both for seedlings and field resistance, MpV.1 and MpII.1 were specific for growth chamber conditions and MpIII.3 and MpIV.1 for field resistance. Quantitative trait loci MpIII.1, MpII.1, MpIII.2 and MpIII.3 may coincide with other QTLs associated with resistance to M. pinodes previously described in P.
sativum. Four QTLs associated with earliness of flowering were also identified. While dfIII.2 and dfVI.1, may correspond with other genes and QTLs controlling earliness in P. sativum, dfIII.1 and dfII.1 may be specific to P. sativum subsp. syriacum. Flowering date and growth habit were strongly associated with resistance to M. pinodes in the field evaluations. The relation observed between earliness, growth habit and resistance to M. pinodes is discussed. 相似文献
13.
Blair MW Muñoz C Garza R Cardona C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(5):913-923
The bean pod weevil (Apion godmani Wagner) is a serious insect pest of common beans (Phaseolus vulgaris L.) grown in Mexico and Central America that is best controlled by host-plant resistance available in Durango or Jalisco
genotypes such as J-117. Given unreliable infestation by the insect, the use of marker-assisted selection is desirable. In
the present study, we developed a set of nine molecular markers for Apion resistance and mapped them to loci on chromosomes
2, 3, 4 and 6 (linkage groups b01, b08, b07and b11, respectively) based on genetic analysis of an F
5:10 susceptible × resistant recombinant inbred line population (Jamapa × J-117) and two reference mapping populations (DOR364 × G19833
and BAT93 × JaloEEP558) for which chromosome and linkage group designations are known. All the markers were derived from randomly
amplified polymorphic DNA (RAPD) bands that were identified through bulked segregant analysis and cloned for conversion to
sequence tagged site (STS) markers. One of the markers was dominant while four detected polymorphism upon digestion with restriction
enzymes. The other markers were mapped as RAPD fragments. Phenotypic data for the population was based on the evaluation of
percentage seed damage in replicated trials conducted over four seasons in Mexico. In single point regression analysis, individual
markers explained from 3.5 to 22.5% of the variance for the resistance trait with the most significant markers overall being
F10-500S, U1-1400R, R20-1200S, W9-1300S and Z4-800S, all markers that mapped to chromosome 2 (b01). Two additional significant
markers, B1-1400R and W6-800R, were mapped to chromosome 6 (b11) and explained from 4.3 to 10.2% of variance depending on
the season. The latter of these markers was a dominant STS marker that may find immediate utility in marker-assisted selection.
The association of these two loci with the Agr and Agm genes is discussed as well as the possibility of additional resistance genes on chromosome 4 (b07) and chromosome 3 (b08).
These are among the first specific markers developed for tagging insect resistance in common bean and are expected to be useful
for evaluating the mechanism of resistance to A. godmani. 相似文献
14.
Common loci underlie field resistance to soybean sudden death syndrome in Forrest,Pyramid, Essex,and Douglas 总被引:8,自引:0,他引:8
Njiti VN Meksem K Iqbal MJ Johnson JE Kassem MA Zobrist KF Kilo VY Lightfoot DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):294-300
Soybean [Glycine max (L.) Merr.] sudden death syndrome (SDS) caused by Fusarium solani f. sp. glycines results in severe yield losses. Resistant cultivars offer the most-effective protection against yield losses but resistant
cultivars such as ’Forrest’ and ’Pyramid’ vary in the nature of their response to SDS. Loci underlying SDS resistance in ’Essex’ × Forrest
are well defined. Our objectives were to identify and characterize loci and alleles that underlie field resistance to SDS
in Pyramid×’Douglas’. SDS disease incidence and disease severity were determined in replicated field trials in six environments
over 4 years. One hundred and twelve polymorphic DNA markers were compared with SDS disease response among 90 recombinant
inbred lines from the cross Pyramid×Douglas. Two quantitative trait loci (QTLs) for resistance to SDS derived their beneficial
alleles from Pyramid, identified on linkage group G by BARC-Satt163 (261-bp allele, P=0.0005, R2=16.0%) and linkage group N by BARC-Satt080 (230-bp allele, P=0.0009, R2=15.6%). Beneficial alleles of both QTLs were previously identified in Forrest. A QTL for re- sistance to SDS on linkage group
C2 identified by BARC-Satt307 (292-bp allele, P=0.0008, R2=13.6%) derived the beneficial allele from Douglas. A beneficial allele of this QTL was previously identified in Essex. Recombinant
inbred lines that carry the beneficial alleles for all three QTLs for resistance to SDS were significantly (P≤0.05) more resistant than other recombinant inbred lines . Among these recombinant inbred lines resistance to SDS was environmentally
stable. Therefore, gene pyramiding will be an effective method for developing cultivars with stable resistance to SDS.
Received: 20 October 1999 / Accepted: 22 May 2001 相似文献
15.
Hayes RJ McHale LK Vallad GE Truco MJ Michelmore RW Klosterman SJ Maruthachalam K Subbarao KV 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(4):509-517
Verticillium wilt of lettuce caused by Verticillium dahliae can cause severe economic damage to lettuce producers. Complete resistance to race 1 isolates is available in Lactuca sativa cultivar (cv.) La Brillante and understanding the genetic basis of this resistance will aid development of new resistant
cultivars. F1 and F2 families from crosses between La Brillante and three iceberg cultivars as well as a recombinant inbred line population derived
from L. sativa cv. Salinas 88 × La Brillante were evaluated for disease incidence and disease severity in replicated greenhouse and field
experiments. One hundred and six molecular markers were used to generate a genetic map from Salinas 88 × La Brillante and
for detection of quantitative trait loci. Segregation was consistent with a single dominant gene of major effect which we
are naming Verticillium resistance 1 (Vr1). The gene described large portions of the phenotypic variance (R
2 = 0.49–0.68) and was mapped to linkage group 9 coincident with an expressed sequence tag marker (QGD8I16.yg.ab1) that has
sequence similarity with the Ve gene that confers resistance to V. dahliae race 1 in tomato. The simple inheritance of resistance indicates that breeding procedures designed for single genes will
be applicable for developing resistant cultivars. QGD8I16.yg.ab1 is a good candidate for functional analysis and development
of markers suitable for marker-assisted selection. 相似文献
16.
W. Cao G. R. Hughes H. Ma Z. Dong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):551-554
The development of Septoria nodorum blotch-resistant cultivars has become a high priority objective for durum wheat breeding programs. Marker-assisted selection
enables breeders to improve selection efficiency. In order to develop markers for resistance to Septoria nodorum blotch, a set of F5 recombinant inbred lines, derived from the crosses Sceptre/3–6, Sceptre/S9–10 and Sceptre/S12–1, was developed based on the
F2-derived family method. Two RAPD markers, designated UBC521650 and RC37510, were detected by bulked segregant analysis and located approximately 15 and 13.1 centiMorgans (cM) from the resistance gene
snbTM, respectively. A SCAR marker was also successfully developed for marker-assisted selection in breeding programs based on
the sequence of the RAPD marker UBC521650. This is the first report of DNA-based markers linked to resistance for Septoria nodorum blotch in durum wheat.
Received: 8 March 2000 / Accepted: 25 June 2000 相似文献
17.
V. Laucou K. Haurogné N. Ellis C. Rameau 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):905-915
A genetic linkage map of Pisum sativum L. was constructed based primarily on RAPD markers that were carefully selected for their reproducibility and scored in a
population of 139 recombinant inbred lines (RILs). The mapping population was derived from a cross between a protein-rich
dry-seed cultivar ‘Térèse’ and an increased branching mutant (K586) obtained from the pea cultivar ‘Torsdag’. The map currently
comprises nine linkage groups with two groups comprising only 6 markers (n=7 in pea) and covers 1139 cM. This RAPD-based map has been aligned with the map based on the (JI281×JI399) RILs population
that currently includes 355 markers in seven linkage groups covering 1881 cM. The difference in map lengths is discussed.
For this alignment 7 RFLPs, 23 RAPD markers, the morphological marker le and the PCR marker corresponding to the gene Uni were used as common markers and scored in both populations.
Received: 13 March 1998 / Accepted: 29 April 1998 相似文献
18.
Kang H Weng Y Yang Y Zhang Z Zhang S Mao Z Cheng G Gu X Huang S Xie B 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(4):795-803
Scab, caused by Cladosporium cucumerinum, is an important disease of cucumber, Cucumis sativus. In this study, we conducted fine genetic mapping of the single dominant scab resistance gene, Ccu, with 148 F9 recombinant inbred lines (RILs) and 1,944 F2 plants derived from the resistant cucumber inbred line 9110Gt and the susceptible line 9930, whose draft genome sequence
is now available. A framework linkage map was first constructed with simple sequence repeat markers placing Ccu into the terminal 670 kb region of cucumber Chromosome 2. The 9110Gt genome was sequenced at 5× genome coverage with the
Solexa next-generation sequencing technology. Sequence analysis of the assembled 9110Gt contigs and the Ccu region of the 9930 genome identified three insertion/deletion (Indel) markers, Indel01, Indel02, and Indel03 that were closely
linked with the Ccu locus. On the high-resolution map developed with the F2 population, the two closest flanking markers, Indel01 and Indel02, were 0.14 and 0.15 cM away from the target gene Ccu, respectively, and the physical distance between the two markers was approximately 140 kb. Detailed annotation of the 180 kb
region harboring the Ccu locus identified a cluster of six resistance gene analogs (RGAs) that belong to the nucleotide binding site (NBS) type R
genes. Four RGAs were in the region delimited by markers Indel01 and Indel02, and thus were possible candidates of Ccu. Comparative DNA analysis of this cucumber Ccu gene region with a melon (C. melo) bacterial artificial chromosome (BAC) clone revealed a high degree of micro-synteny and conservation of the RGA tandem repeats
in this region. 相似文献
19.
A novel locus for clubroot resistance in<Emphasis Type="Italic"> Brassica rapa</Emphasis> and its linkage markers 总被引:2,自引:0,他引:2
Hirai M Harada T Kubo N Tsukada M Suwabe K Matsumoto S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(4):639-643
An inbred turnip (Brassica rapa syn. campestris) line, N-WMR-3, which carries the trait of clubroot resistance (CR) from a European turnip, Milan White, was crossed with a clubroot-susceptible doubled haploid line, A9709. A segregating F3 population was obtained by single-seed descent of F2 plants and used for a genetic analysis. Segregation of CR in the F3 population suggested that CR is controlled by a major gene. Two RAPD markers, OPC11-1 and OPC11-2, were obtained as candidates of linkage markers by bulked segregant analysis. These were converted to sequence-tagged site markers, by cloning and sequencing of the polymorphic bands, and named OPC11-1S and OPC11-2S, respectively. The specific primer pairs for OPC11-1S amplified a clear dominant band, while the primer pairs for OPC11-2S resulted in co-dominant bands. Frequency distributions and statistical analyses indicate the presence of a major dominant CR gene linked to these two markers. The present marker for CR was independent of the previously found CR loci, Crr1 andCrr2. Genotypic distribution and statistical analyses did not show any evidence of CR alleles on Crr1 andCrr2 loci in N-WMR-3. The present study clearly demonstrates that B. rapa has at least three CR loci. Therefore, the new CR locus was named Crr3. The present locus may be useful in breeding CR Chinese cabbage cultivars to overcome the decay of present CR cultivars.Communicated by C. Möllers 相似文献
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Falke KC Melchinger AE Flachenecker C Kusterer B Frisch M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(5):857-866
Intermated mapping populations are expected to result in high mapping resolution for tightly linked loci. The objectives of our study were to (1) investigate the consequences of constructing linkage maps from intermated populations using mapping methods developed for F2 populations, (2) compare linkage maps constructed from intermated populations (F2Syn3) with maps generated from corresponding F2 and F3 base populations, and (3) investigate the advantages of intermated mapping populations for applications in plant breeding programs. We constructed linkage maps for two European flint maize populations (A × B, C × D) by mapping 105 SSR markers in generations F2 and F2Syn3 of population A × B, and 102 SSR markers in generations F3 and F2Syn3 of population C × D. Maps for F2Syn3 were constructed with mapping methods for F2 populations (Map A) as well as with those specifically developed for intermated populations (Map B). Both methods relate map distances to recombination frequencies in a single meiosis and, therefore, did not show a map expansion in F2Syn3 compared with maps constructed from the respective F2 or F3 base populations. Map A and B differed considerably, presumably because of theoretical shortcomings of Map A. Since loosely linked markers could not unambiguously be mapped in the F2Syn3 populations, they may hamper the construction of linkage maps from intermated populations. 相似文献