河南新乡段卫河水对黑斑侧褶蛙早期胚胎发育的影响

研究了不同浓度新乡卫河水对黑斑侧褶蛙早期胚胎的发育毒性.结果表明:黑斑侧褶蛙胚胎在卫河水原水中不能存活;56%浓度卫河水可使胚胎畸形率和死亡率显著升高;低浓度(10%、18%、32%)卫河水能明显抑制胚胎全长的增加.在研究污染物尤其是低浓度污染物对黑斑侧褶蛙胚胎发育毒性时,生长抑制参数是比致死率和畸形率更为敏感的终点参数.卫河污染严重,存在潜在致畸变物质,应加速卫河治理.     

Effects of post-ovulatory food deprivation on the hormonal profiles, activity of the oviduct and ova transport in sows

The objective of the present study was to investigate the effect of post-ovulatory food deprivation on the hormonal profiles and consequently on the activity of the oviduct and ova transport in sows. Sows were randomly allocated to the control (C-group, n=6) or fasted (F-group, n=5) group. The F-group sows were fasted for four meals starting with the morning meal after detection of ovulation in the second oestrus after weaning. Ovulation was checked by transrectal ultrasonography. Blood was collected for the analyses of progesterone, oestradiol-17beta, prostaglandin F(2 approximately ) metabolite, insulin, free fatty acids and triglycerides. Oviductal isthmic motility was monitored on Polyview before and after ovulation until the time of slaughter. After slaughter, the isthmus opposite the side with transducer was divided into three equal segments and flushed separately and a third of the uterine horn part from the utero-tubal-junction (UTJ) was also flushed. A high proportion of ova in the F-group was found in the first and second parts of the isthmus. In the C-group, a high proportion of ova was found in the third part of the isthmus and the uterus. The mean isthmic pressure in the C-group decreased significantly (P<0.05) during the period immediately after ovulation while in the F-group mean pressure remained unchanged. The frequency of phasic pressure fluctuations were significantly (P<0.05) higher in the F- than in the C-group 13 to 24 h after ovulation. No significant differences in progesterone concentrations were seen between the two groups of sows. Prostaglandin metabolite levels were significantly (P<0.05) higher in the F-group than in the C-group. Oestradiol-17beta levels significantly (p<0.05) decreased earlier in the F- than in the C-group. Serum insulin levels were significantly (p=0.05) lower in the F- than in the C-group while free fatty acids were significantly (p<0.01) higher in the F- than in the C-group. There were no significant differences in the serum levels of triglycerides between the F- and the C-group. Therefore, it can be concluded in the present study that food deprivation is associated with changes in the hormonal profiles, activity of the oviduct and a delay of ova transport in sows.     

Supplemental progesterone during early pregnancy exerts divergent responses on embryonic characteristics in sows and gilts

Progesterone (P4) plays a key role in pregnancy establishment and maintenance; during early pregnancy, P4 stimulates the production and release of uterine secretions necessary for conceptus growth prior to implantation; therefore, exogenous P4 supplementation may improve embryo development. This study evaluated the effects of supplementation during early pregnancy with long-acting injectable progesterone or altrenogest on embryonic characteristics of sows and gilts. Thus, a total of 32 sows and 16 gilts were used. On day 6 of pregnancy sows and gilts were allocated to one of the following groups: non-supplemented; supplemented with 20 mg of altrenogest, orally, from days 6 to 12 of pregnancy; supplemented with 2.15 mg/kg of long-acting injectable progesterone on day 6 of pregnancy. Animals were killed on day 28 of pregnancy, and ovulation rate, embryo survival, embryo weight, crown-to-rump length, uterine glandular epithelium and endometrial vascularization were assessed. Treatments had no effect on pregnancy rate, embryo survival or endometrial vascular density (P > 0.05). Non-supplemented gilts presented larger and heavier embryos compared to gilts from supplemented groups (P < 0.05). Sows in the altrenogest group presented larger and heavier embryos compared to non-supplemented sows and sows supplemented with long-acting injectable progesterone. In conclusion, supplementation of sows and gilts with progestagen from day 6 of pregnancy can be used as a means to improve embryo survival without deleterious effects.     

Inductive interactions in early embryonic development.

This is an update of a previous review (Current Opinion in Cell Biology 2:969-974) in which we discussed recent work attempting to understand the sequence of inductive interactions responsible for establishing the body plan of the early embryo. As before, we concentrate on inductive interactions in amphibian embryos, where significant progress has been made in the past two years. In this update, however, we also consider recent embryological data obtained with amniote embryos such as the chick, together with complementary data provided by genetic analyses of mouse and Drosophila development.     

'Cleavage fields': hypothesis on early embryonic development

The hypothesis is put forward that events of the early embryonic development can be interpreted on the basis of a radial distribution of cytoplasmic components, i.e. of a 'cleavage field', progressively established during the growth of the oocyte. The orientations of the cleavage spindles and the corresponding furrows' positions are assumed to be correlated to the field's temporal evolution which, in turn, is determined by flows of cytoplasmic components originated by the changes in the membrane shape. From this viewpoint, a simple explanation of egg regulation is proposed, and the particular case of the sea urchin embryo is briefly discussed.     

Clinical features and hormonal profiles of cloprostenol-induced early abortions in heifers monitored by ultrasonography

Background  

The present study describes the clinical features and plasma profiles of bovine pregnancy-associated glycoprotein 1 (bPAG1), the main metabolite of prostaglandin F_2α (PG metabolite) and progesterone (P4) in heifers in which early abortions were induced.     

早期小鼠胚胎发育的基因表达

受精是新个体发育的时 -空点 ,从一个形态单纯的单细胞受精卵发育成能独立生活的个体动物 ,形态上出现一系列的变化 ,而更重要的是基因表达。基因组是机体内惟一确定的 ,为所有各类细胞共同拥有 ,但基因组内各个基因表达的选择性和程度随时间、位置和环境条件的不同而发生改变 (严云勤等 ,2 0 0 2 ;胡静等 ,2 0 0 1;范衡宇等 ,2 0 0 1)。个体的发育和分化、内环境的稳定性、对外界刺激的应答、细胞循环的调节、衰老和程序化细胞死亡等正常的发育过程以及疾病的病理学过程 ,包括癌症的病理学过程 ,无论是由一个基因的突变引起的或是由于多基…     

Effect of feeding level on progesterone concentration in early pregnant multiparous sows

The effect of three feeding regimens on progesterone level was tested during early pregnancy in multiparous sows. A total of eighteen sows in their eighth parity (8.1 +/- 2.8, mean +/- S.D.) were used. During lactation the sows were fed to appetite and after weaning they received 4 kg (52 MJ) a commercial feed per day. Following ovulation, sows were allocated to one of three treatment groups and fed 2 kg/day (low feeding, LLL) or 4 kg/day (high feeding, HHH) throughout the trial or 2 kg/day for 11 days, 4 kg/day for 10 days, and 2 kg/day for the remaining days of the study (modified feeding, LHL). Blood for progesterone and cortisol analyses was collected daily throughout the study, and for luteinizing hormone (LH) assay for 12 h at 15 min intervals on days 14 and 21 of pregnancy. An adrenocorticotropic hormone (ACTH) challenge test was performed on all sows day 28 of pregnancy. Dietary treatment did not significantly affect hormonal parameters. However, progesterone concentration tended to be lower (P = 0.08) in the HHH group than in the LLL group. In the LHL group venous progesterone concentration seemed to fluctuate. No effects of feeding were observed on progesterone concentration in allantoic fluid on day 35 of pregnancy. Venous cortisol level was significantly higher (P < 0.05) during proestrus and oestrus in all groups and there was no significant difference between groups in response to ACTH challenge. The mean amplitude of LH pulses decreased significantly (P < 0.01) from days 14 to 21 of pregnancy in all groups. In addition, an interaction was found between feeding level and baseline LH concentration and also between feeding level and mean LH concentration. Embryonic recovery was highest in the LLL (69%), lowest in the HHH (45%) and moderate in the LHL (55%) group. Neither high feeding nor modified feeding provided any benefits for reproductive performance in multiparous sows. A low feeding regimen thus appears optimal for multiparous sows in early pregnancy at least with the management regime described.     

Emerging asymmetry and embryonic patterning in early mouse development

Recent studies have revealed asymmetries in the mouse zygote and preimplantation embryo, well before the establishment of anterior-posterior polarity after implantation. Whether these asymmetries are causally related to embryonic patterning or are coincidental outcomes of the topology of normal development remains uncertain.     

Fertilization and early embryonic development in androstenedione-immunized Merino ewes

Ewes were immunized against androstenedione (Fecundin) and assigned to be mated 14 days (179 ewes Group C) or 25 days (174 ewes Group B) after a booster immunization with Fecundin. The anti-androstenedione titres at these times were 6790 and 3240 respectively (P less than 0.01). The remaining 169 ewes were untreated controls (Group A). Ewes were mated to entire rams (12 rams to 180 ewes) at their second oestrus after synchronization of oestrus. Immunization against androstenedione caused a shortening of the time from sponge removal to mating (Day 0) and a decrease in the percentage of ewes mated by the rams. Also, ovulation rate was increased after immunization (P less than 0.01), being 1.42, 2.16 and 1.93 for Groups A, C and B respectively. Egg recovery rates on Day 2 were lower in immunized ewes and there was some indication that fertilization rates were lowered. On Day 13 after mating a higher proportion of blastocysts was recovered from ewes in Group A than from those in Groups B and C. Immunization resulted in lower fertilization rates and smaller blastocysts with lower mitotic indexes (P less than 0.01). At Days 24-32 of pregnancy fetal weight was lower in the immunized ewes. At all sampling stages, the proportion of ewes pregnant (fertility) was lowered in immunized ewes. The results of the present study show that significant reproductive wastage occurs in androstenedione-immunized Merino ewes, with lower rates of embryo recovery and delayed embryonic development being found in comparison to controls.(ABSTRACT TRUNCATED AT 250 WORDS)     

Retinoic acid synthesis and functions in early embryonic development

Retinoic acid (RA) is a morphogen derived from retinol (vitamin A) that plays important roles in cell growth, differentiation, and organogenesis. The production of RA from retinol requires two consecutive enzymatic reactions catalyzed by different sets of dehydrogenases. The retinol is first oxidized into retinal, which is then oxidized into RA. The RA interacts with retinoic acid receptor (RAR) and retinoic acid X receptor (RXR) which then regulate the target gene expression. In this review, we have discussed the metabolism of RA and the important components of RA signaling pathway, and highlighted current understanding of the functions of RA during early embryonic development.     

Effects of estradiol and progesterone on early embryonic development in aging rats

Regularly cyclic, middle-aged female rats exhibit a decreased incidence of fertility, and those females that are fertile produce small litters. These decreases in fertility and litter size are associated with reduced numbers of normal blastocysts formed and implanted, suggesting that pre- and/or peri-implantation failures may be the causes for these aging-related reproductive declines. The present study examined the relationships and influence of circulating estradiol (E2) and progesterone (P) levels on early embryonic development and implantation in middle-aged rats. Serial blood samples obtained from cannulated, middle-aged pregnant rats revealed minor decreases in plasma P and increases in E2 levels during Days 2-4 of pregnancy, compared to young pregnant rats, resulting in significantly (p less than 0.001) decreased plasma P/E2 ratios. These alterations in endogenous hormone secretion in middle-aged pregnant rats were associated with fewer normal blastocysts on Day 5 of pregnancy and reduced numbers of normally implanting embryos. Correlation analysis further revealed a significant (p less than 0.05) inverse relationship between mean circulating E2 levels and numbers of normal conceptuses on Day 12 of gestation. Moreover, s.c. administration of P implants (in Silastic) to middle-aged pregnant rats increased serum P levels by about 34-40 ng/ml, and significantly (p less than 0.05) reduced the incidence of abnormal embryos before implantation. In contrast, treatment with E2 minipumps produced a sustained rise in serum E2 (by about 7-15 pg/ml) and resulted in the complete absence of embryos in the reproductive tracts by Day 5 of pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)     

Influence of exogenous progesterone on early embryonic development in the mare

The influence of exogenous progesterone on the development of equine oviductal embryos was determined based upon the recovery of Day-7 uterine blastocysts from treated mares (n=13) that were given 450 mg progesterone daily between Days 0 and 6 and from untreated control mares (n=13). Daily administration of 450 mg progesterone in oil significantly (P<0.02) increased serum progesterone concentrations in the treated mares. There was no significant difference in the recovery rate of Day-7 embryos between treated and control mares (8/13 versus 6/13, respectively). Embryonic development, assessed by morphologic evaluation, embryo diameter, and number of cell nuclei was not significantly different for embryos from treated and from control mares. The results of this study indicate that administration of progesterone beginning on the day of ovulation does not affect the embryo recovery rate or embryonic development, based on evaluation of uterine blastocysts recovered at Day 7 after ovulation.     

Roles of cell junctions in gametogenesis and in early embryonic development

Recent reviews of the role of cell junctions in development have focused primarily upon functions related to the relatively subtle physiological modulation of their subunits in relation to fundamental developmental processes in a wide variety of organisms. There is, however, considerable support from numerous laboratories that the more radical modulation of the presence and number of junctional subunits in many diverse tissues may play a pivotal role in a wide spectrum of developmental phenomena ranging from gametogenesis to organogenesis. Since a great deal of recent interest in this latter subject has concentrated upon vertebrate systems including mammals, this review will examine the functional significance of the modulation of gap junctions, tight junctions and desmosomes in a developing idealized mammalian system from gamete formation to tissue and organ differentiation during embryo-genesis.