Photosynthetic rates of citronella and lemongrass

Ten selections of citronella (Cymbopogon nardus [L.] Rendle) were grown at 32/27, 27/21, or 15/10 C day/night temperatures, and plants from three populations of lemongrass (Cymbopogon citratus [D.C.] Stapf from Japan or Sri Lanka and Cymbopogon flexuosus [D.C.] Stapf from India) were grown at 8- or 15-hour photoperiods. Net photosynthetic rates of mature leaves were measured in a controlled environment at 25 C and 260 microeinsteins per meter² per second. Rates declined with increasing leaf age, and from the tip to the base of the leaf blade. Rates for citronella leaves grown at 15/10 C were extremely low for all selections. Highest rates of net photosynthesis were recorded for four selections grown at 27/21 C and for two selections grown at 32/27 C. Lemongrass grown at 8-hour photoperiod had higher photosynthetic rates than that grown at 15-hour photoperiod.     

Behavior,net reproduction,longevity, and mummy-stage survival ofAphidius matricariae [Hym. Aphidiidae]

The courtship, mating and ovipositional behavior ofA. matricariae Haliday were studied. UsingMyzus persicae (Sulzer) as the host, the production of progeny per female parasite and survival from mummy stage to the adult were studied at constant temperatures of 10°, 12.8°, 15.6°, 18.3°, 21°, 24°, 26.7°, 29.5° and 32°C. The longevity of male and female parasites was determined at temperatures of 7°, 10°, 15.6°, 21°, 26.7°, 29.5° and 32°C. The greatest number of progeny (392) was produced at 21°C. The optimal temperatures for production of progeny and survival of the parasites during the mummy stage were from 12.8°C to 21°C. The longevity of male and female adult parasites decreased as temperatures increased and male parasites lived significantly (P<0.05) longer than females at 10° and 15.6°C.     

Effect of temperature and humidity on the development ofPhytoseiulus Persimilis and its ability to regulate populations ofTetranychus urticae [Acarina: Phytoseiidae. Tetranychidae]

The development ofPhytoseiulus persimilis Athias-Henriot and the effectiveness of it as a predator ofTetranychus urticae (Koch) were studied at constant temperatures of 15°, 18°, 21°, 24° and 27°C (humidity fluctuations from 60% to 90% R.H.) and at constant humidities of 40% and 80% R.H. at the temperatures 21° and 27°. Optimal temperature for time of development was 27° (at 60%–85% R.H.). A high reduction in egg vitality was recorded at 40% R. H. and 27% At 21° the egg vitality was only slightly lower at 40% R.H. than at 80% R.H. The predator gave control ofT. urticae at temperatures from 15° to 27° (humidity fluctuation from 60%–90% R.H.), and the most rapid and efficient control was obtained at 27° (60%–85% R.H.). The predator did not give sufficient control ofT. urticae at 27° and 40% R.H. At 21° control ofT. urticae was obtained at both 40% and 80% R.H., but the prey population was reduced faster at 80% R.H. than at 40% R.H.     

Effects of temperature and dosage on Vairimorpha sp. 696 spore morphometrics,spore yield,and tissue specificity in Heliothis virescens

The effects of temperature and dosage on a new microsporidian species, Vairimorpha sp. 696, were examined in H. virescens. The pathogen was evaluated for tissue specificity, spore size, cumulative percentage mortality, and spore production. All tissues examined bore infection at 32°C. Spore length was significantly longer at 19°C (5.9 μm) than at 32°C (4.7 μm). Spore widths at these two temperatures did not differ significantly. Octospores were not found at either temperature at 8 or 12 days postinoculation. One hundred percent mortality was attained in all dosages administered, but the initial rate of mortaily was more rapid in the higher dosages. Finally, spore yield was greater in larvae administered lower dosages. Maximum spore yield at 27°C was 4.87 × 10⁹ spores/larva.     

Effect of temperature onin vitro pollen germination in pigeonpea

Pollen of pigeonpea(Cajanus cajan (L.) Millsp.) cultivars H-77-216 and ICPL-151 were cultivatedin vitro at six different temperatures (12, 17, 22, 27, 32, 37 °C). Pollen of cv. H-77-216 started to germinate at 17 °C whereas the pollen of cv. ICPL-151 at 22 °C, the optimal temperatures were 22 and 27 °C, respectively. Pollen germination at different temperatures was found to be positively correlated with the tube length. Per cent pollen bursting increased with rising temperature. The indeterminate cv. H-77-216 showed a wide range of suitable temperatures (17 – 27 °C) for pollen germination while the determinate cv. ICPL-151 had optimum at 27 °C     

Growth at 37 degrees C of the EGs strain of the amoeboflagellate Naegleria gruberi containing viruslike particles. I. Nuclear changes

Naegleria gruberi amoebae, EG_s strain, containing viruslike particles (VLP) were grown at temperatures of 21° and 37°C. At 21°C, the amoebae displayed the morphological structures associated with development of the VLP's. At 37°C, however, gross morphological modifications and new structures appeared. When amoebae were at 37°C for less than 12 hr, nuclei were found to have a larger number of VLP's than amoebae at 21°C. Exposure of the amoebae to the higher temperature for 12–24 hr resulted in a scarcity of particles. Large bundles of microtubulelike fibrils were present in the nucleoplasm of amoebae at 37°C, and, in addition, the nuclei showed degenerative modifications. The fibrillar changes were not due to the elevated temperature alone since a substrain of EG_s (=EG_B) not infected with VLP's exhibited no nuclear modifications. It is assumed that the elevated temperature accelerated and enhanced a lytic effect of the VLP's upon the cells.     

Influence of soil temperature on niacin and thiamine in honey mesquite

Summary The influence of soil temperature was examined on niacin and thiamine concentration in honey mesquite (Prosopis glandulosa var.glandulosa) seedlings. The seedlings were grown in soil temperature regimes of 21, 27, and 32°C in a controlled environment growth room. Nodulation randomly occurred on the roots of the seedlings, necessitating separate analysis according to the occurrence of nodulation. Roots of nodulated seedlings from the 21°C soil temperature regime contained greater quantities of niacin and thiamine compared to root samples from seedlings grown in either 27 or 32°C regimes. Niacin concentration of non-nodulated seedlings was highest in samples from seedlings grown in the 27°C soil temperature regime and lowest in samples from seedlings grown in the 21°C regime. Thiamine concentration was the greatest from non-nodulated seedlings grown in the 27°C soil temperature regime, while the thiamine concentration of non-nodulated samples from the 32°C regime was the least. Optimal soil temperature for honey mesquite root growth appears to be about 27°C. At sub-optimal soil temperatures niacin might have limited ‘growth’ while at supra-optimal soil temperatures, thiamine might be a limiting factor. College of Agricultural Sciences Contribution No. T-9-164.     

Influence of different temperatures on the tachinid parastite,Sturmiopsis inferens [Dip.]

The influence of constant temperatures of 27, 29, 31 and 33°C and alternating temperature of 31/33°C (18/6 h) onSturmiopsis inferens Townsend was studied during 12 successive generations. The larval and pupal periods for male parasites were 13.5±0.5 and 11.0±0.3 days respectively and for female 12.8±0.5 and 11.1±0.3 days respectively in the 1st generatioin at 27°C. It decreased progressively with increase in temperature. Survival of females, fertility and fecundity were adversely affected at higher temperatures. A temperature range of 27–29°C appeared to be optimum for mass rearing of the parasite in the laboratory. The higher premature mortality observed at a constant 33°C was not observed at temperatures fluctuating between 31/33°C. Presumably under field conditions, where temperature is constantly fluctuating, the flies will be able to withstand a comparatively higher temperature.     

The kinetics of light-induced changes of C-550, cytochrome b559 and fluorescence yield in chloroplasts at low temperature

The kinetics of the photoreduction of C-550, the photooxidation of cytochrome b₅₅₉ and the fluorescence yield changes during irradiation of chloroplasts at ?196 °C were measured and compared. The photoreduction of C-550 proceeded more rapidly than the photooxidation of cytochrome b₅₅₉ and the fluorescence yield increase followed the cytochrome b₅₅₉ oxidation. These results suggest that fluorescence yield under these conditions indicates the dark reduction of the primary electron donor to Photosystem II, P680⁺, by cytochrome b₅₅₉ rather than the photoreduction of the primary electron acceptor.The photoreduction of C-550 showed little if any temperature dependence over the range of ?196 to ?100 °C. The amount of cytochrome b₅₅₉ photooxidized was sensitive to temperature decreasing from the maximal change at temperatures between ?196 to ?160 °C to no change at ?100 °C. To the extent that the reaction occurred at temperatures between ?160 and ?100 °C the rate was largely independent of temperature. The rate of the fluorescence increase was dependent on temperature over this range being 3–4 times more rapid at ?100 than at ?160 °C. At ?100 °C the light-induced fluorescence increase and the photoreduction of C-550 show similar kinetics. The temperature dependence of the fluorescence induction curve is attributed to the temperature dependence of the dark reduction of P680⁺.The intensity dependence of the photoreduction of C-550 and of the photooxidation of cytochrome b₅₅₉ are linear at low intensities (below 200 μW/cm²) but fall off at higher intensities. The failure of reciprocity in the photoreduction of C-550 at the higher intensities is not explained by the simple model proposed for the Photosystem II reaction centers.     

Time of sowing and temperature effects on the flowering of Stylosanthes guianensis

Seedlings of Stylosanthes guianensis var. guianensis cv. Cook and of two selections of S. guianensis var. pauciflora (CIAT 1280 and CIAT 1062) were grown at day/night temperatures of 20°/25°, 30°/25°, and 35°/30°C in a naturally-lit glasshouse at latitude 27°30'S. Sowings were made in decreasing daylength at 30-day intervals from 22 January to 21 May. Cv. Cook and the CIAT 1280 selection did not flower fully if sown after 22 January and the CIAT 1062 selection did not flower if sown after 22 March. This is interpreted as a long-short day flowering response. Usually, flowers were initiated earlier and at a lower node at 25?/20°C than at the warmer temperatures. At 25?/20°C the first flowers to appear were produced predominantly at the terminal apex of the main stem in cv. Cook and the CIAT 1062 selection, but not in the CIAT 1280 selection. At the two warmer temperatures first flowers were more commonly produced at the terminal apex of primary, secondary and tertiary branches. There were more inflorescences per plant on earlier than later sown plants when measured 21 days from appearance of the first flower and the most inflorescences were produced by cv. Cook at 25?/20°C, by selection CIAT 1062 at 30°/25°C, and by CIAT 1280 at 35°/30°C.     

Carbon dioxide compensation values in citronella and lemongrass

Carbon dioxide compensation values of mature leaves from 10 selections of citronella (Cymbopogon nardus [L.] Rendle) grown at 32/27 or 27/21 C day/night temperatures and three strains of lemongrass (Cymbopogon citratus [D.C.] Stapf. and Cymbopogon flexuosus [D.C.] Stapf.) grown at 8- or 15-hour photoperiods were measured in a controlled environment at 25 C. All leaves had low compensation values but citronella varied from 1.3 to 9.7 μl/liter and lemongrass from 0.7 to 3.5 μl/liter. Lower growing temperature generally resulted in lower compensation values for citronella but there was no consistent photoperiod effect on lemongrass.     

Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37° C and 27° C

The rate of synthesis and degradation of phospholipids in Mycobacterium smegmatis ATCC 607, grown at 27° C and 37° C was studied by incorporation of ³²P into phospholipids and chase of radioactivity of the pulse-labelled phospholipids. A relatively low rate of synthesis and degradation of phospholipids in cells growth at 27° C was observed as compared to those grown at 37° C. Phosphatidylethanolamine (PE) had the maximum turnover at 37° C. However, at 27° C, cardiolipin (CL) showed a turnover rate higher than PE. Phosphatidylinositol mannosides (PIMs) were metabolically more active at 37° C than at 27° C. The differences in metabolic activity of the phospholipids at the two temperatures have been discussed.     

Effect of egg storage duration and brooding temperatures on chick growth,intestine morphology and nutrient transporters

The effects of egg storage duration (ESD) and brooding temperature (BT) on BW, intestine development and nutrient transporters of broiler chicks were investigated. A total of 396 chicks obtained from eggs stored at 18°C for 3 days (ESD3-18°C) or at 14°C for 14 days (ESD14-14°C) before incubation were exposed to three BTs. Temperatures were initially set at 32°C, 34°C and 30°C for control (BT-Cont), high (BT-High) and low (BT-Low) BTs, respectively. Brooding temperatures were decreased by 2°C each at days 2, 7, 14 and 21. Body weight was measured at the day of hatch, 2, 7, 14, 21, 28 and 42. Cloacal temperatures of broilers were recorded from 1 to 14 days. Intestinal morphology and gene expression levels of H⁺-dependent peptide transporter (PepT1) and Na-dependent glucose (SGLT1) were evaluated on the day of hatch and 14. Cloacal temperatures of chicks were affected by BTs from days 1 to 8, being the lowest for BT-Low chicks. BT-High resulted in the heaviest BWs at 7 days, especially for ESD14-14°C chicks. This result was consistent with longer villus and larger villus area of ESD14-14°C chicks at BT-High conditions. From 14 days to slaughter age, BT had no effect on broiler weight. ESD3-18°C chicks were heavier than ESD14-14°C chicks up to 28 days. The PepT1 and SGLT1 expression levels were significantly higher in ESD3-18°C chicks than ESD14-14°C on the day of hatch. There was significant egg storage by BT interaction for PepT1 and SGLT1 transporters at day 14. ESD14-14°C chicks had significantly higher expression of PepT1 and SGLT1 at BT-Low than those at BT-Cont. ESD14-14°C chicks upregulated PepT1 gene expression 1.15 and 1.57-fold at BT-High and BT-Low, respectively, compared with BT-Cont, whereas PepT1 expression was downregulated 0.67 and 0.62-fold in ESD3-18°C chicks at BT-High and BT-Low. These results indicated that pre-incubation egg storage conditions and BTs affected intestine morphology and PepT1 and SGLT1 nutrient transporters expression in broiler chicks.     

The Influence of Temperature on Development and Sex Differentiation of Meloidogyne graminis

Meloidogyne grarninis (Sledge and Golden) Whitehead on Cynodon sp. (var. ''Tifgreen'' bermudagrass) was studied at four temperatures; 16, 21, 27, and 32 C. Both mode and rate of development were temperature dependent. Females developed more rapidly and in greater numbers at 27 C: saccate females exuding matrices were present 14 days following inoculation, eggs were laid after 21 days and newly-hatched larvae were present in the matrix at 25 days. Sex differentiation to males was 80% at 32 C and 4% at 27 C. No males were observed at 21 or 16 C. Developing males were present 14 days following inoculation and emerged from roots after 21 days at 32 C. In populations pre-exposed to 27 C then transferred to 32 C, the percentage of males ranged from 0 for 1 day exposure at the initial temperature to 45.5% after 5 days. After 11 days pre-exposure the recovery of males was 4.3%. Individuals interpreted to be male sex reversals and male intersexes were noted. Pre-exposure at 32 C for 1 or 2 days followed by 27 C produced 1-2% males, while exposure for 3 or more days at 32 C followed by 27 C produced 90% males.     

Effect of Temperature on Development and Reproduction of <Emphasis Type="Italic">Epilachna dodecastigma</Emphasis> (Wied.) (Coleoptera: Coccinellidae)

The effect of five constant temperatures of 21, 24, 27, 30 and 33 °C on adult life span, reproduction, oviposition behavior and larval developmental time of a bitter gourd inhabited coleopteran insect Epilachna dodecastigma (Wied.) (Coccinellidae) was determined in laboratory conditions under 70 ± 5 % relative humidity and a photoperiod of 12 L : 12 D. Larval developmental time of E. dodecastigma decreased as temperature increased from 21 to 33 °C. Life table data revealed that overall mortality was lowest at 27 °C and highest at 21 °C. Females lived longer than males at all temperatures; but longevity decreased with increase in temperature. Pre-oviposition period decreased significantly with increase in temperature up to 27 °C and thereafter increased at a slower rate; whereas oviposition period decreased significantly with increase in temperature. Fecundity and egg viability increased significantly with an increase in temperature up to 27 °C and thereafter decreased at a slower rate. The intrinsic rate of increase (r _m ) was 0.1703, 0.1984, 0.2235, 0.2227 and 0.2181 day^?1 at 21, 24, 27, 30 and 33 °C, respectively. The net reproductive rate and finite rate of increase was highest at 27 °C (R _o  = 112.05; λ = 1.4233) and lowest at 21 °C (R _o  = 51.23; λ = 1.2581).     

Development ofDiatraea Saccharalis [Lep.: Pyralidae] at constant temperatures

At constant temperatures between 15.6 and 32°C the incubation time of eggs ofDiatraea saccharalis (F.) was reduced by each increase in temperature. At 34°C the time decreased. Highest (98.6%) and lowest (9.9%) egg hatch occurred at 26 and 34°C, respectively. Larvae completed development at temperatures ranging from 22 to 34°C; however, only 4.4% of the larvae pupated at 34°C. Duration of the larval stage at 30°C (♂=18.1 days; ♀=19.1 days) was ca. 14 days shorter than at 22°C. Maximum rate of development in the pupal stage occurred at 28°C (ca. 6.8 days), and a higher temperature increased developmental time and mortality. Adult longevity and egg production generally were reduced with increasing temperatures and egg production was highest at 24°C (729.8 eggs/ moth). As many as 7 larval stages occurred; but most larvae completed development in 5 stages, and none completed development in less than 5 stages. The female larval stage was ca. 1 day longer than that of males, and this difference occurred primarily in the 5th stage.     

Growth and photosynthesis in seedlings of Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta) cultured at two different temperatures

This study examined temperature acclimation, growth, and photosynthetic characteristics of the zygote-derived seedlings of Hizikia fusiformis (Harvey) Okamura (Sargassaceae). The seedlings were cultured at 15°C or 25°C for 4 weeks. The average relative growth rate was significantly higher in seedlings acclimated at 25°C. The photosynthetic rate measured at 15°C was much higher in seedlings grown at 15°C than those grown at 25°C, indicating photosynthetic acclimation to a lower temperature. At 35°C, the photosynthetic rate of 15°C-grown seedlings was drastically decreased, whereas that of 25°C-grown seedlings was significantly increased. The maximum relative electron transport rate (rETR_max) measured at the respective growth temperature was significantly higher in seedlings grown at 25°C than at 15°C. At a measuring temperature of 35°C, the rETR_max in both 15°C- and 25°C-grown seedlings were considerably reduced with regard to those measured at 15°C or 25°C. Our results suggested that, compared with the seedlings grown at 25°C, those acclimated at a lower temperature could be disadvantaged under adverse conditions such as increased temperatures.     

Growth and development of Hymenolepis nana in mice maintained at different environmental temperatures

Growth and development of Hymenolepis nana in mice maintained at different environmental temperatures. International Journal for Parasitology16: 13–17. On days 3 and 4 post infection (p.i.) the number of Hymenolepis nana cysticercoids in the villi of male mice kept at 5°C did not differ from those in controls (21°C), but fewer larvae were observed in hosts at 35°C. However, on day 10 and 14 p.i. in cysticercoid and egg-induced infections respectively, the incidence of infection was higher and significantly more worms per host were found in mice at 5°C than in those kept at 21 or 35°C. Also, worms from mice maintained at 5°C were significantly heavier and became patent 1 day earlier than those from 21°C, which, in turn, were significantly heavier than those grown in hosts at 35°C.     

Timelapse scanning reveals spatial variation in tomato (Solanum lycopersicum L.) root elongation rates during partial waterlogging

Aims

Effects of soil amendments with crop residues on suppression of damping-off of sugar beet were examined by growing the seedlings in pasteurized, Rhizoctonia solani (AG2-2 IIIB)-infested soil at different temperatures. Dried residues of five dasheen or taro (Colocasia esculenta (L.) Schott) cultivars were compared with those of peanut (Arachys hypogaea L.) and Brassica rapa Olsson for their effects on disease suppression.

Methods and Results

When the seedlings were grown at 17/12 °C (day/night), all residues equally suppressed the disease when amended into the soil just before sowing. At 22/17 or 32/27 °C, damping-off developed in non-treated soil within 10 days, and differential suppressive effects by the residues became apparent by 21 days. When non-pasteurized, non-treated soil was infested with the pathogen, seedling survival was markedly better than in the same but pasteurized, infested soil. Yet, the effect was not different within the entire temperature ranges. Growth of both R. solani and the seedlings peaked near 25 °C and leveled off at higher temperatures.

Conclusions

These results suggest that damping-off was suppressed by antagonistic soil microorganisms, and individual residues elevated their effects differently. Under cool conditions, the antagonists dominated the pathogen to suppress the disease. Under warmer conditions, pathogenesis overcame antagonism depending on the residue, resulting in differential effects of disease suppression.     

EVOLUTIONARY ADAPTATION TO TEMPERATURE. VI. PHENOTYPIC ACCLIMATION AND ITS EVOLUTION IN ESCHERICHIA COLI

Acclimation refers to reversible, nongenetic changes in phenotype that are induced by specific environmental conditions. Acclimation is generally assumed to improve function in the environment that induces it (the beneficial acclimation hypothesis). In this study, we experimentally tested this assumption by measuring relative fitness of the bacterium Escherichia coli acclimated to different thermal environments. The beneficial acclimation hypothesis predicts that bacteria acclimated to the temperature of competition should have greater fitness than do bacteria acclimated to any other temperature. The benefit predicted by the hypothesis was found in only seven of 12 comparisons; in the other comparisons, either no statistically demonstrable benefit was observed or a detrimental effect of acclimation was demonstrated. For example, in a lineage evolutionarily adapted to 37°C, bacteria acclimated to 37°C have a higher fitness at 32°C than do bacteria acclimated to 32°C, a result exactly contrary to prediction; acclimation to 27°C or 40°C prior to competition at those temperatures confers no benefit over 37°C acclimated forms. Consequently, the beneficial acclimation hypothesis must be rejected as a general prediction of the inevitable result of phenotypic adjustments associated with new environments. However, the hypothesis is supported in many instances when the acclimation and competition temperatures coincide with the historical temperature at which the bacterial populations have evolved. For example, when the evolutionary temperature of the population was 37°C, bacteria acclimated to 37°C had superior fitness at 37°C to those acclimated to 32°C; similarly, bacteria evolutionarily adapted to 32°C had a higher fitness during competition at 32°C than they did when acclimated to 37°C. The more surprising results are that when the bacteria are acclimated to their historical evolutionary temperature, they are frequently competitively superior even at other temperatures. For example, bacteria that have evolved at either 20°C or 32°C and are acclimated to their respective evolutionary temperatures have a greater fitness at 37°C than when they are acclimated to 37°C. Thus, acclimation to evolutionary temperature may, as a correlated consequence, enhance performance not only in the evolutionary environment, but also in a variety of other thermal environments.