共查询到20条相似文献,搜索用时 15 毫秒
1.
T. Tai B. J. Staskawicz 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(1):112-117
A yeast artificial chromosome (YAC) library was constructed using high-molecular-weight DNA isolated from pepper (Capsicum annuum L.) leaf protoplasts. Insert DNA was prepared by partial digestion using EcoRI and subjected to electrophoretic fractionation before in-gel ligation to the pJS97/98 YAC vector. Prior to transformation
of yeast spheroplasts, ligation products were subjected to a second electrophoretic size selection. The library consists of
about 19 000 clones with an average insert size of 500 kb, thus representing approximately three haploid genome equivalents.
Three PCR-based markers tightly linked to the pepper Bs2 resistance gene were used to assess the utility of this library for positional cloning. Three YAC clones containing pepper
genomic DNA from the Bs2 resistance locus were isolated from the library. The clones ranged in size from 270 kb to 1.2 Mb and should prove useful
for the cloning of the Bs2 gene.
Received: 15 January 1999 / Accepted: 11 May 1999 相似文献
2.
M. Pierre L. Noël T. Lahaye A. Ballvora J. Veuskens M. Ganal U. Bonas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):255-263
The pepper (Capsicum annuum) Bs3 gene confers resistance to Xanthomonas campestris pv vesicatoria strains expressing the avirulence protein AvrBs3. Using amplified fragment length polymorphism (AFLP) and bulked DNA templates
from resistant and susceptible plants we identified markers linked to Bs3 and defined a 2.1-cM interval containing the target gene. Bs3-linked AFLP fragments were cloned and conformity of isolated PCR products with the desired markers was determined by hybridisation
to membrane-bound AFLP reactions. AFLP markers flanking the target gene were converted into locus-specific PCR-based markers.
These markers were employed for the analysis of 790 plants segregating for Bs3, resulting in a linkage map with a genetic resolution of 0.13 cM. Mapping of Bs3-linked markers in tomato placed them to a syntenic interval on tomato chromosome 2.
Received: 15 October 1999 / Accepted: 29 November 1999 相似文献
3.
C. Djian-Caporalino L. Pijarowski A. Fazari M. Samson L. Gaveau C. O’Byrne V. Lefebvre C. Caranta A. Palloix P. Abad 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):592-600
The PM687 line of Capsicum annuum L. has a single dominant gene, Me
3
, that confers heat-stable resistance to root-knot nematodes (RKN). Me
3
was mapped using doubled-haploid (DH) lines and F2 progeny from a cross between the susceptible cultivar ’Yolo Wonder’ (’YW’) and the highly resistant line ’PM687’. Bulked-segregant
analysis with DNA pools, from susceptible or resistant DH lines, was performed to identify RAPD and AFLP markers linked to
Me
3
. There was no polymorphism between bulks of ten DH lines using over 800 RADP primers (4,000 amplified fragments analysed).
Using 512 AFLP primers (74,000 amplified fragments analysed), and bulked DNA templates from 20 resistant and 20 susceptible
plants, we identified eight repulsion-phase and four coupling-phase markers linked to Me
3.
Analysed in 103 DH progeny, they defined a 56.1-cM interval containing the target gene. The nearest were located 0.5, 1.0,
1.5 and 3.0 centimorgans (cM) on both sides of the gene. Analysis of the F2 progeny (162 plants) with the nearest coupling-phase marker confirmed its close position. Another resistance gene to RKN,
present in ’PM687’ (Me
4
), was shown to be linked to Me
3
, 10 cM from it. In order to localize Me
3
and Me
4
on our reference intraspecific pepper linkage map, two AFLP markers were mapped. The Me
3
nearest marker was 10.1cM from a RAPD marker named Q04_0.3 and 2.7cM from a RFLP marker named CT135. We investigated map-position
orthologies between Me
3
and two other nematode resistance genes, the tomato Mi-3 and the potato Gpa
2
genes, which mapped in the telomeric region of the short arm of the tomato and potato chromosome 12 (or XII for potato).
Received: 23 March 2000 / Accepted: 2 January 2001 相似文献
4.
T. Lahaye S. Hartmann S. Töpsch A. Freialdenhoven M. Yano P. Schulze-Lefert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):526-534
The Mla-12-mediated resistance in barley against Erysiphe graminis f. sp. hordei requires for its function the Rar1 gene. High-resolution genetic mapping was accomplished by inspecting more than 4000 plants segregating for Rar1 within an 0.7-cM interval containing the target gene. Marker enrichment in the target region was carried out by an amplified
fragment length polymorphism (AFLP)-based search for polymorphic loci using bulked DNA templates from resistant and susceptible
recombinants adjacent to Rar1. RFLP markers closely linked to Rar1 were used to investigate the relationship between physical and genetical distances by PFGE Southern analysis, indicating
the physical linkage of two genetically separated RFLP loci. Comparative mapping of Rar1-linked RFLP probes in barley and rice identified a break of collinearity in the orthologous chromosome segments.
Received: 11 February 1998 / Accepted: 3 March 1998 相似文献
5.
Isolation and FISH mapping of Yeast Artificial Chromosomes (YACs) encompassing an allele of the Gm2 gene for gall midge resistance in rice 总被引:2,自引:0,他引:2
K. R. Rajyashri S. Nair N. Ohmido K. Fukui N. Kurata T. Sasaki M. Mohan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):507-514
Ten yeast artificial chromosomes (YACs) spanning the Gm2 locus have been isolated by screening high-density filters containing a total of approximately 7000 YAC (representing six
genome equivalents) clones derived from a japonica rice, Nipponbare. The screening was done with five RFLP markers flanking a gall midge resistance gene, Gm2, which was previously mapped onto chromosome 4 of rice. This gene confers resistance to biotype 1 and 2 of gall midge (Orseolia oryzae), a major insect pest of rice in South and Southeast Asia. The RFLP markers RG214, RG329 and F8 hybridized with YAC Y2165.
Two overlapping YAC clones (Y5212 and Y2165) were identified by Southern hybridization, with Gm2-flanking RFLP markers, and their inserts isolated. The purified YACs and RFLP markers flanking Gm2 were labeled and physically mapped by the fluorescence in situ hybridization (FISH) technique. All of them mapped to the long arm of chromosome 4 of the resistant variety of rice, ‘Phalguna’,
confirming the previous RFLP mapping data.
Received: 15 December 1997 / Accepted: 5 March 1998 相似文献
6.
M. L. Xu S. S. Korban 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):844-851
Using the amplified fragment length polymorphism (AFLP) technique combined with a ”narrow-down” bulk segregant strategy enabled
us to quickly identify 15 tightly linked AFLP markers to the Vf gene that confers resistance to the apple scab disease. High-resolution mapping placed all 15 AFLP markers within an interval
of 0.6 cM around the Vf region; 7 of them were found to be inseparable from the Vf gene, 1 was localized left of, and the remaining 7 located right of the Vf gene. In addition, eight previously identified RAPD markers were also mapped, but only three, including M18, AM19, and AL07,
were localized within this short interval, and none co-segregated with the Vf gene. The saturation of the Vf region with AFLP markers will accelerate both marker-assisted selection and map-based cloning. The advantages of this ”narrow-down”
strategy, estimation of physical distances among AFLP markers, and their potential application are also discussed.
Received: 22 December 1999 / Accepted: 25 March 2000 相似文献
7.
Y. Hayano-Saito K. Saito S. Nakamura S. Kawasaki M. Iwasaki 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):59-63
The Stvb-i gene confers stripe disease resistance to rice. For positional cloning, we constructed a physical map spanning 1.8-cM distance
between flanking markers, consisting of 18 bacterial artificial chromosome (BAC) clones, around the Stvb-i locus on rice chromosome 11. The 18 clones were isolated by screening a BAC library derived from a japonica cultivar, Shimokita, with three Stvb-i-linked RFLP markers and DraI-digested DNAs of a yeast artificial chromosome (YAC) clone. The results of Southern hybridization and restriction enzyme
analyses indicated that these BAC clones are contiguous and cover about a 700-kb region containing the Stvb-i allele. Utilizing end and internal fragments of the BAC insert DNAs, 33 molecular markers were generated within a small chromosomal
region including the Stvb-i locus. Genotyping analysis with these markers for a resistant cultivar and four nearby recombinants selected from 120 F2 individuals indicated that Stvb-i is contained within an approximately 286-kb region covered with two overlapping BAC clones.
Received: 25 August 1999 / Accepted: 16 November 1999 相似文献
8.
Genetic and physical mapping of xa13, a recessive bacterial blight resistance gene in rice 总被引:6,自引:0,他引:6
A. C. Sanchez L. L. Ilag D. Yang D. S. Brar F. Ausubel G. S. Khush M. Yano T. Sasaki Z. Li N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1022-1028
The recessive gene, xa13, confers resistance to Philippine race 6 (PXO99) of the bacterial blight pathogen Xanthomonas oryzae pv oryzae. Fine genetic mapping and physical mapping were conducted as initial steps in an effort to isolate the gene. Using nine selected
DNA markers and two F2 populations of 132 and 230 plants, xa13 was fine-mapped to a genomic region <4 cM on the long arm of rice chromosome 8, flanked by two RFLP markers, RG136 and R2027.
Four DNA markers, RG136, R2027, S14003, and G1149, in the target region were used to identify bacterial artificial chromosome
(BAC) clones potentially harboring the xa13 locus from a rice BAC library. A total of 11 BACs were identified, forming four separate contigs including a single-clone
contig, 29I3, associated with the RG136 STS marker, the S14003 contig consisting of four clones (44F8, 41O2, 12A16, and 12F20),
the G1149 contig with two clones, 23D11 and 21H18, and the R2027 contig consisting of four overlapping clones, 42C23, 30B5,
6B7 and 21H14. Genetic mapping indicated that the xa13 locus was contained in the R2027 contig. Chromosomal walking on the R2027 contig resulted in two more clones, 33C7 and 14L3.
DNA fingerprinting showed that the six clones of the R2027 contig were overlapping. Clone 44F8 hybridized with a single fragment
from the clone 14L3, integrating the R2027 and S14003 contigs into a single contig consisting of ten BAC clones with a total
size of approximately 330 kb. The physical presence of the xa13 locus in the contig was determined by mapping the ends of the BAC inserts generated by TAIL-PCR. In an F2 population of 230 plants, the BAC-end markers 42C23R and 6B7F flanked the xa13 locus. The probes 21H14F and 21H14R derived from BAC clone 21H14 were found to flank xa13 at a distance of 0.5 cM on either side, using a second F2 population of 132 plants. Thus, genetic mapping indicated that the contig and the 96-kb clone, 21H14, contained the xa13 locus.
Received: 15 August 1998 / Accepted: 29 September 1998 相似文献
9.
X. Li H. J. van Eck J. N. A. M. Rouppe van der Voort D.-J. Huigen P. Stam E. Jacobsen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1121-1128
Due to the complexity of tetrasomic inheritance, mapping studies in potato (Solanum tuberosum L.) are generally conducted at the diploid level. In the present study we tested the feasibility of Bulked Segregant Analysis
(BSA) using a tetraploid offspring for the identification of AFLP markers linked to the R2 allele, which confers race-specific resistance to Phytophthora infestans. Eleven bulk-specific AFLP markers, detected in fingerprints of 205 AFLP primer combinations, could be mapped in a linkage
group encompassing the R2 locus. The efficiency of BSA at the tetraploid level, determined by the frequency of single-dose restriction fragments (SDRF),
was much higher than expected on the basis of overall genetic dissimilarity between the parental clones. The fortuitous detection
of AFLPs with linkage to the R2 allele is explained on the basis of specific genetic dissimilarity between cultivated potato and the chromosomal segment
introgressed from S. demissum carrying the resistant R2 allele. AFLP markers common to those with linkage to R2 were visually recognized by their electrophoretic mobility in the AFLP fingerprint in a parental clone of a reference mapping
population. Using these common AFLP markers we anchored the linkage group comprising the R2 allele to potato chromosome 4.
Received: 30 October 1997 / Accepted: 6 November 1997 相似文献
10.
Development of RAPD and SCAR markers linked to the Russian wheat aphid resistance gene Dn2 in wheat 总被引:5,自引:0,他引:5
A. A. Myburg M. Cawood B. D. Wingfield A.-M. Botha 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1162-1169
RAPD (random amplified polymorphic DNA) analysis was used to identify molecular markers linked to the Dn2 gene conferring resistance to the Russian wheat aphid (Diuraphis noxia Mordvilko). A set of near-isogenic lines (NILs) was screened with 300 RAPD primers for polymorphisms linked to the Dn2 gene. A total of 2700 RAPD loci were screened for linkage to the resistance locus. Four polymorphic RAPD fragments, two in
coupling phase and two in repulsion phase, were identified as putative RAPD markers for the Dn2 gene. Segregation analysis of these markers in an F2 population segregating for the resistance gene revealed that all four markers were closely linked to the Dn2 locus. Linkage distances ranged from 3.3 cM to 4.4 cM. Southern analysis of the RAPD products using the cloned RAPD markers
as probes confirmed the homology of the RAPD amplification products. The coupling-phase marker OPB10880c and the repulsion-phase marker OPN1400r were converted to sequence characterized amplified region (SCAR) markers. SCAR analysis of the F2 population and other resistant and susceptible South African wheat cultivars corroborated the observed linkage of the RAPD
markers to the Dn2 resistance locus. These markers will be useful for marker-assisted selection of the Dn2 gene for resistance breeding and gene pyramiding.
Received: 1 July 1997 / Accepted: 20 October 1997 相似文献
11.
Targeted resistance gene mapping in soybean using modified AFLPs 总被引:7,自引:0,他引:7
A. J. Hayes M.A. Saghai Maroof 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(8):1279-1283
The soybean [Glycine max (Merr.) L.] linkage group F contains a vital region of clustered genes for resistance to numerous pathogens including the
soybean mosaic virus resistance gene, Rsv1. In order to develop new genetic markers that map to this gene cluster, we employed a targeted approach that utilizes the
speed and high-throughput of AFLP, but modified it to incorporate sequence information from the highly conserved nucleotide
binding site (NBS) region of cloned disease resistance genes. By using a labeled degenerate primer corresponding to the p-loop
portion of the NBS region of resistance genes, such as N, L6, and Rps2, we were able to quickly amplify numerous polymorphic bands between parents of a population segregating for resistance to
Rsv1. Of these polymorphic bands, bulk segregant analysis revealed four markers that were closely linked to Rsv1. These markers were cloned and used as probes for RFLP analysis. The four clones mapped to within a 6-cM region surrounding
Rsv1, the closest being 0.4 cM away from the gene. Sequence analysis showed that all four clones contain the p-loop sequence
corresponding to the degenerate primer and that one of the four clones contains an open reading frame sequence which when
translated is related to the NBS region of other cloned disease resistance genes. The rapid identification of four markers
closely linked to Rsv1 in soybean demonstrates the utility of this method for generating markers tightly linked to important plant disease resistance
genes.
Received: 25 September 1999 / Accepted: 3 November 1999 相似文献
12.
High-resolution linkage analysis and physical characterization of the EIX-responding locus in tomato
M. Ron R. Kantety G. B. Martin N. Avidan Y. Eshed D. Zamir A. Avni 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):184-189
An ethylene-inducing xylanase (EIX) from Tricohoderma viride is a potent elicitor of ethylene biosynthesis, localized cell death and other defense responses in specific cultivars of
tobacco (Nicotiana
tabacum) and tomato (Lycopersicon esculentum). Wild species of tomato, such as Lycopersicon cheesmanii and Lycopersicon pennellii, do not respond to EIX treatment. The F1 progeny of a L. esculentum×L. cheesmanii and a L. esculentum×L. pennellii cross responded to EIX treatment with an increase in ethylene biosynthesis and the induction of localized cell death. The
F2 progeny of the above mentioned crosses segregated 3:1 (responding:non-responding). We mapped the EIX-responding locus (Eix) to the short arm of chromosome 7 using a population of introgression lines (ILs), containing small RFLP-defined chromosome
segments of L. pennellii introgressed into L. esculentum. RFLP analysis of 990 F2 plants that segregated for the introgressed segment mapped the Eix locus 0.1 cM and 0.9 cM from the flanking markers TG61 and TG131, respectively. Using the marker TG61 we isolated a yeast
artificial chromosome (YAC) clone that carries 300-kb DNA segments derived from the Eix region. By mapping the ends of this YAC clone we show that it spans the Eix locus. Thus, positional cloning of the Eix locus appears feasible.
Received: 20 March 1999 / Accepted: 30 April 1999 相似文献
13.
Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanese larch 总被引:25,自引:0,他引:25
A. Arcade F. Anselin P. Faivre Rampant M. C. Lesage L. E. Pâques D. Prat 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):299-307
Genetic linkage maps have been increasingly developed for a wide variety of plants, using segregating populations such as
F2s or backcrosses between inbred lines. These pedigrees are rarely available in outbred species like forest trees which have
long generation times. Thus genetic mapping studies have to use peculiar pedigrees and markers in appropriate configurations.
We constructed single-tree genetic linkage maps of European larch (Larix decidua Mill.) and Japanese larch [Larix kaempferi (Lamb.) Carr.] using segregation data from 112 progeny individuals of an hybrid family. A total of 266 markers (114 AFLP,
149 RAPD and 3 ISSR loci) showing a testcross configuration, i.e.heterozygous in one parent and null in the other parent,
were grouped at LOD 4.0, θ=0.3. The maternal parent map (L. decidua)consisted of 117 markers partitioned within 17 linkage groups (1152 cM) and the paternal parent map (L. kaempferi) had 125 markers assembled into 21 linkage groups (1206 cM). The map distance covered by markers was determined by adding
a 34.7-cM independence distance at the end of each group and unlinked marker. It reached 2537 cM and 2997 cM respectively
for European larch and Japanese larch, and represented respectively a 79.6% and 80.8% coverage of the overall genome. A few
3:1 segregating markers were used to identify homologous linkage groups between the European larch and the Japanese larch
genetic maps. The PCR-based molecular markers allowed the construction of genetic maps, thus ensuring a good coverage of the
larch genome for further QTL detection and mapping studies.
Received: 15 March 1999 / Accepted: 29 March 1999 相似文献
14.
Y. Ohta S. J. Powis W. John Coadwell Darren E. Haliniewski Y. Liu Hong Li M. F. Flajnik 《Immunogenetics》1999,49(3):171-182
The amphibian Xenopus laevis is one non-mammalian vertebrate in which the major histocompatibility complex (MHC) has been analyzed extensively. Class
IIβ, class Ia, LMP2, LMP7, HSP70, C4, Factor B, and Ring3 genes have been identified and mapped to the MHC. Here, we report the isolation of a transporter associated with antigen
processing (TAP) gene, TAP2, and demonstrate its linkage to the MHC. While the ATP-binding region of Xenopus
TAP2 is highly conserved in evolution, amino acid identity to other vertebrate TAP proteins was not detected in the N-terminal
region. Segregation analysis of 34 individuals from two families showed exact restriction fragment length polymorphism matching
between the MHC class Ia gene and the one TAP2 gene demonstrating linkage conservation since the mammalian/amphibian divergence ∼350 million years ago. In addition, one
non-MHC-linked TAP2–hybridizing fragment was detected in approximately half of the individuals tested. Interestingly, TAP2 allelic lineages appear to match those of LMP7 and classical class I, which previously were categorized into two highly divergent groups that emerged at least 60 million
years ago. Similar to LMP7 and class Ia,TAP2 is expressed ubiquitously with highest levels in intestine and spleen.
Received: 2 March 1998 / Revised: 15 July 1998 相似文献
15.
Z. Deng Q. Tao Y.-L. Chang S. Huang P. Ling C. Yu C. Chen F. G. Gmitter Jr. H.-B. Zhang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1177-1184
A BAC library was constructed from the genomic DNA of an intergeneric Citrus and Poncirus hybrid. The library consists of 24,576 clones with an average insert size of 115 kb, representing approximately seven haploid
genome equivalents and is able to give a greater than 99% probability of isolating single-copy citrus DNA sequences from this
library. High-density colony hybridization-based library screening was performed using DNA markers linked to the citrus tristeza
virus (CTV) resistance gene and citrus disease resistance gene candidate (RGC) sequences. Between four and eight clones were
isolated with each of the CTV resistance gene-linked markers, which agrees with the library’s predicted genome coverage. Three
hundred and twenty-two clones were identified using 13 previously cloned citrus RGC sequences as probes in library screening.
One to four fragments in each BAC were shown to hybridize with RGC sequences. One hundred and nine of the RGC BAC clones were
fingerprinted using a sequencing gel-based procedure. From the fingerprints, 25 contigs were assembled, each having a size
of 120–250 kb and consisting of 2–11 clones. These results indicate that the library is a useful resource for BAC contig construction
and molecular isolation of disease resistance genes.
Received: 22 May 2000 / Accepted: 25 September 2000 相似文献
16.
High-resolution genetic map of the Lv resistance locus in tomato 总被引:3,自引:0,他引:3
J. Chunwongse S. Doganlar C. Crossman J. Jiang S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):220-223
Bulked segregant analysis and high-resolution mapping were used to pinpoint the position of the Lv gene for resistance to Leveillula taurica in tomato. Mapping in an F2, corresponding to more than 3800 gametes, indicates that Lv is positioned within the 0.84-cM interval defined by the RFLP markers CT121 and CT129, with the closest marker, CT121, being
only 0.16 cM from the gene. The tight linkage of these markers demonstrates their usefulness in marker-assisted breeding for
Lv, and the high-resolution map provides a starting a starting point for positional cloning of this resistance gene.
Received: 25 February 1997 / Accepted: 21 March 1997 相似文献
17.
Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice 总被引:9,自引:0,他引:9
J. H. Jia D. S. Zhang C. Y. Li X. P. Qu S. W. Wang V. Chamarerk H. T. Nguyen B. Wang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):607-612
TGMS (thermo-sensitive genic male-sterile) rice is widely used in hybrid rice production. Because of a specific temperature
requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered reverse thermo-sensitive genic
male-sterile line, J207S, has an opposite phynotype compared to the normal TGMS lines. J207S is completely sterile when the
temperature is lower than 31°C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility
of J207S was controlled by a single recessive gene which was first named as rtms1. An F2 population from the cross between J207S and E921 was developed and used for molecular mapping of the rtms1 gene. The AFLP (amplified fragment length polymorphism) technique, combined with BSA (bulked segregant analysis), was used
to screen markers linked to the target gene, and eight polymorphic AFLP loci were identified. Co-segregating analysis using
the F2 population showed that two of them, Rev1 and Rev7, were closely linked to the target gene with a recombinant rate of 3.8%
and 7.7%, respectively. Both Rev1 and Rev7 were found to be single-copy sequences through Southern analysis. Rev1 was subsequently
mapped on chromosome 10 with a doubled-haploid mapping populations derived from the cross CT9993 × IR62266 available at Texas
Tech University. RM222 and RG257 were linked to Rev1 at a distance of 11.8 cM and 4.6 cM, respectively. Additional SSR markers
from the rice map of Cornell University, RFLP markers from the map of RGP in Japan and the map of Texas Tech University were
selected from the region surrounding Rev1 on chromosome 10 to conduct the fine-mapping of the rtms1 gene. Presently, rtms1 was mapped between RM239 and RG257 with genetic distance of 3.6 cM and 4.0 cM, respectively. The most-closely linked AFLP
marker, Rev1, 4.2 cM from the rtms1 gene, was sequenced and converted into a SCAR (sequence characterized amplified region) marker which could facilitate marker-assisted
selection of the rtms1 gene.
Received: 2 November 2000 / Accepted: 21 November 2000 相似文献
18.
Deletion-based physical mapping of barley chromosome 7H 总被引:1,自引:1,他引:0
N. Serizawa S. Nasuda F. Shi T. R. Endo S. Prodanovic I. Schubert G. Künzel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(6-7):827-834
Chromosomal mutations in barley (Hordeum vulgare, 2n=2x=14, HH) chromosome 7H added to the common wheat (Triticum aestivum, 2n=6x=42, AABBDD) cultivar Chinese Spring were induced genetically by the gametocidal activity of certain alien chromosomes derived
from wild species of the genus Aegilops. The rearranged barley chromosomes were characterized by C-banding, FISH and GISH. Twenty two deletion or translocation chromosomes
in a hemizygous condition were selected for deletion mapping of 17 AFLP and 28 STS markers that are specific to 7H. Of the
22 breakpoints in chromosome 7H, seven involved the short arm (7HS), 12 the long arm (7HL) and three were in the centromeric
region. The seven 7HS breakpoints separated all four 7HS-specific AFLP markers and split the 21 STS markers into six groups.
One breakpoint occurred between two STS markers formerly occupying the same position in the genetic map. All seven 7HS breakpoints
were separated from each other by either the AFLP or STS markers. The 12 breakpoints in 7HL divided the 13 7HL-specific AFLP
markers into seven groups, and the seven STS markers into three groups. On the other hand, the 12 breakpoints in 7HL were
divided into six groups by the AFLP markers and into two groups by the STS markers. This deletion-based map was in accordance
with previously published genetic and physical maps using the same STS markers. The breakpoints, AFLP markers and STS markers
were arrayed in a consistent order.
Received: 5 February 2001 / Accepted: 19 February 2001 相似文献
19.
RFLP mapping of resistance to chlorosis induction by Pyrenophora tritici-repentis in wheat 总被引:2,自引:0,他引:2
J. D. Faris J. A. Anderson L. J. Francl J. G. Jordahl 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):98-103
Tan spot, caused by Pyrenophora tritici-repentis, is an economically important disease in major wheat production areas. The fungus can produce two genetically distinct symptoms
on leaves of susceptible wheat genotypes: tan necrosis (nec) and extensive chlorosis (chl). Our objectives were to determine
the number of genes conditioning resistance to tan spot in a population of wheat recombinant inbred lines, and map the chromosomal
location of the resistance genes using RFLPs. Conidia produced by the P. tritici-repentis isolate Pti2 (nec+chl+) were used to inoculate seedlings of 135 recombinant inbred lines derived from the cross of the synthetic
hexaploid wheat W-7984 with Opata 85. A subset of the population was inoculated with conidia produced by the isolates D308
(nec−chl+) and 86-124 (nec+chl−). Inoculated seedlings were rated on a scale of 1 to 5 based on lesion type. Necrosis-inducing
culture filtrate produced by the isolate 86-124 was also used to screen the entire population. A map consisting of 532 markers
was employed to identify significant associations between marker loci and tan spot resistance. The entire population was insensitive
to culture filtrate produced by the isolate 86-124, and the entire subset was resistant to conidial inoculation of the same
isolate. The population segregated for reaction to isolates D308 and Pti2, indicating that this population segregates for
resistance to extensive chlorosis only, and not to tan necrosis. RFLP analysis indicated the presence of a gene with a major
effect in 1AS, a gene with a minor effect in 4AL, and an interaction between the 1AS gene and a gene in 2DL. Together, these
loci explained 49.0% of the variation in this population for resistance to tan spot produced by the isolate Pti2. Two regions
one in 1BL and one in 3BL, were significantly associated with resistance to extensive chlorosis, but were not significant
in the multiple regression model. It should be feasible to introgress these resistance loci into adapted genetic backgrounds
by using a marker-assisted selection scheme.
Received: 30 March 1996 / Accepted: 31 May 1996 相似文献
20.
Identification of a STS marker linked to the Aegilops speltoides-derived leaf rust resistance gene Lr28 in wheat 总被引:7,自引:0,他引:7
S. Naik K. S. Gill V. S. Prakasa Rao V. S. Gupta S. A. Tamhankar S. Pujar B. S. Gill P. K. Ranjekar 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):535-540
A sequence-tagged-site (STS) marker is reported linked to Lr28, a leaf rust resistance gene in wheat. RAPD (random amplified polymorphic DNA) analysis of near-isogenic lines (NILs) of
Lr28 in eight varietal backgrounds was carried out using random primers. Genomic DNA enriched for low-copy sequences was used
for RAPD analysis to overcome the lack of reproducibility due to the highly repetitive DNA sequences present in wheat. Of
80 random primers tested on the enriched DNA, one RAPD marker distinguished the NILs and the donor parent from the susceptible
recurrent parents. The additional band present in resistant lines was cloned, sequenced, and STS primers specific for Lr28 were designed. The STS marker (Indian patent pending: 380 Del98) was further confirmed by bulk segregation analysis of F3 families. It was consistently present in the NILs, the resistant F3 bulk and the resistant F3 lines, but was absent in recurrent parents, the susceptible F3 bulk and the susceptible F3 lines.
Received: 20 February 1998 / Accepted: 4 March 1998 相似文献