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K. Ba Y. Fu X. Wei Y. Yue G. Li Y. Yao J. Chen X. Cai C. Liang Y. Ge Y. Lin 《Cell proliferation》2013,46(3):312-319
Objective
The aim of this study was to investigate effects of low‐intensity pulsed ultrasound (LIPUS) on differentiation of adipose‐derived stem cells (ASCs), in vitro.Materials and methods
Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR‐γ1, and APN, was examined by real‐time PCR. Immunofluorescence (IF) staining was performed to test for PPAR‐γ at the protein level.Results
Our data revealed that specific patterns of LIPUS up‐regulated levels of both PPAR‐γ1 and APN mRNA, and PPAR‐γ protein.Conclusions
In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.2.
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Low‐intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling
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![点击此处可从《Journal of cellular biochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Kenichi Matsumoto Tsuyoshi Shimo Naito Kurio Tatsuo Okui Soichiro Ibaragi Yuki Kunisada Kyoichi Obata Masanori Masui Pang Pai Yuu Horikiri Nobuyuki Yamanaka Masaharu Takigawa Akira Sasaki 《Journal of cellular biochemistry》2018,119(6):4352-4360
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Yu Suk Choi Gregory J. Dusting Samantha Stubbs Sandeep Arunothayaraj Xiao Lian Han Philippe Collas Wayne A. Morrison Rodney J. Dilley 《Journal of cellular and molecular medicine》2010,14(4):878-889
Human adipose‐derived stem cells (ASCs) may differentiate into cardiomyocytes and this provides a source of donor cells for tissue engineering. In this study, we evaluated cardiomyogenic differentiation protocols using a DNA demethylating agent 5‐azacytidine (5‐aza), a modified cardiomyogenic medium (MCM), a histone deacetylase inhibitor trichostatin A (TSA) and co‐culture with neonatal rat cardiomyocytes. 5‐aza treatment reduced both cardiac actin and TropT mRNA expression. Incubation in MCM only slightly increased gene expression (1.5‐ to 1.9‐fold) and the number of cells co‐expressing nkx2.5/sarcomeric α‐actin (27.2%versus 0.2% in control). TSA treatment increased cardiac actin mRNA expression 11‐fold after 1 week, which could be sustained for 2 weeks by culturing cells in cardiomyocyte culture medium. TSA‐treated cells also stained positively for cardiac myosin heavy chain, α‐actin, TropI and connexin43; however, none of these treatments produced beating cells. ASCs in non‐contact co‐culture showed no cardiac differentiation; however, ASCs co‐cultured in direct contact co‐culture exhibited a time‐dependent increase in cardiac actin mRNA expression (up to 33‐fold) between days 3 and 14. Immunocytochemistry revealed co‐expression of GATA4 and Nkx2.5, α‐actin, TropI and cardiac myosin heavy chain in CM‐DiI labelled ASCs. Most importantly, many of these cells showed spontaneous contractions accompanied by calcium transients in culture. Human ASC (hASC) showed synchronous Ca2+ transient and contraction synchronous with surrounding rat cardiomyocytes (106 beats/min.). Gap junctions also formed between them as observed by dye transfer. In conclusion, cell‐to‐cell interaction was identified as a key inducer for cardiomyogenic differentiation of hASCs. This method was optimized by co‐culture with contracting cardiomyocytes and provides a potential cardiac differentiation system to progress applications for cardiac cell therapy or tissue engineering. 相似文献
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Letícia D. Lucke Fernanda O. Bortolazzo Viviane Theodoro Lucas Fujii Andr L. Bombeiro Maíra Felonato Rodrigo A. Dalia Giane D. Carneiro Luciana P. Cartarozzi Cristina Pontes Vicente Alexandre L. R. Oliveira Fernanda A. S. Mendona Marcelo A. M. Esquisatto Edson R. Pimentel Andrea A. de Aro 《Cell proliferation》2019,52(3)
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Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation
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![点击此处可从《Journal of cellular physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Viviana Costa Valeria Carina Simona Fontana Angela De Luca Francesca Monteleone Stefania Pagani Maria Sartori Stefania Setti Cesare Faldini Riccardo Alessandro Milena Fini Gianluca Giavaresi 《Journal of cellular physiology》2018,233(2):1558-1573
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Derek A. Banyard Ara A. Salibian Alan D. Widgerow Gregory R. D. Evans 《Journal of cellular and molecular medicine》2015,19(1):21-30
Adipose‐derived stem cells (ADSCs) are a subset of mesenchymal stem cells (MSCs) that possess many of the same regenerative properties as other MSCs. However, the ubiquitous presence of ADSCs and their ease of access in human tissue have led to a burgeoning field of research. The plastic surgeon is uniquely positioned to harness this technology because of the relative frequency in which they perform procedures such as liposuction and autologous fat grafting. This review examines the current landscape of ADSC isolation and identification, summarizes the current applications of ADSCs in the field of plastic surgery, discusses the risks associated with their use, current barriers to universal clinical translatability, and surveys the latest research which may help to overcome these obstacles. 相似文献
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Jennifer Petsche Connell Emily Augustini Kenneth J. Moise Jr. Anthony Johnson Jeffrey G. Jacot 《Journal of cellular and molecular medicine》2013,17(6):774-781
Amniotic fluid‐derived stem cells (AFSC) have been reported to differentiate into cardiomyocyte‐like cells and form gap junctions when directly mixed and cultured with neonatal rat ventricular myocytes (NRVM). This study investigated whether or not culture of AFSC on the opposite side of a Transwell membrane from NRVM, allowing for contact and communication without confounding factors such as cell fusion, could direct cardiac differentiation and enhance gap junction formation. Results were compared to shared media (Transwell), conditioned media and monoculture media controls. After a 2‐week culture period, AFSC did not express cardiac myosin heavy chain or troponin T in any co‐culture group. Protein expression of cardiac calsequestrin 2 was up‐regulated in direct transmembrane co‐cultures and media control cultures compared to the other experimental groups, but all groups were up‐regulated compared with undifferentiated AFSC cultures. Gap junction communication, assessed with a scrape‐loading dye transfer assay, was significantly increased in direct transmembrane co‐cultures compared to all other conditions. Gap junction communication corresponded with increased connexin 43 gene expression and decreased phosphorylation of connexin 43. Our results suggest that direct transmembrane co‐culture does not induce cardiomyocyte differentiation of AFSC, though calsequestrin expression is increased. However, direct transmembrane co‐culture does enhance connexin‐43‐mediated gap junction communication between AFSC. 相似文献