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1.
A comprehensive range of phenylacetic acids substituted with nitro, halogen, methyl, amino, hydroxyl and N-acetylamino groups have been synthesized and their growth-regulating activities assessed in the wheat cylinder, pea curvature and pea segment tests. The influence of substituents on molecular shape is shown to be more important in determining activity than their effects on electron distribution. Studies with 2,6-disubstituted phenylacetic acids have indicated that the most active compounds can attain a certain spatial configuration in which one surface of the molecule, including the plane of the ring system, is flat and the carboxyl group is above with its axis of rotation perpendicular to this surface. Positional requirements for growth-regulating activity in phenylacetic acids are shown to be less important than in the phenoxyacetic and benzoic acids. 相似文献
2.
Wheat, pea and dwarf bean seedlings grown under controlled environmental conditions were used to assess the growth-retardant activity of members of series of chloro-substituted benzyl-, trimethyl- and tri-n-butyl- ammonium bromides. The influence on activity of trialkyl groupings other than tri-methyl and tri-n-butyl was also studied using compounds with the 4-chloro-benzyl ring structure. 3-Chloro- and 4-chloro-benzyltributylammonium bromides were the most effective compounds. The activity was similar to that of 2,4-dichlorobenzyltributylphosphonium bromide (Phosphon-D) and they showed little phytotoxicity. A series of chlorophenoxymethyltributylammo-nium and phosphonium salts were found to have lower activity than the corresponding chlorobenzyl derivatives. Allyldimethylsulphonium bromide retarded the growth of wheat seedlings but, like the aliphatic trimethylam-monium bromides tested, it was only slightly active in the pea-seedling test. The results are considered in relation to the chemical structure of the compounds studied. In particular, the influence of chlorine substitution in the ring of benzyltributylammonium salts on their growth-retardant activity is compared with the effect of similar substitution on the auxin activity of phenoxyacetic acids. 相似文献
3.
The relation of naturally acquired host IgG in the surface coat of bloodstream forms of Trypanosoma lewisi to ablastin was studied to determine whether, contrary to a long-held conclusion, the antibody is avid and adsorbable. It was found by immunofluorescence and agglutination tests with monospecific antisera to rat IgG that bloodstream forms collected from immunosuppressed hosts, in contrast to those from immunocompetent hosts, have little or no detectable surface IgO. Specificity of adsorption was also demonstrated in other immunofluorescence experiments in which bloodstream forms from immunosuppressed hosts adsorbed IgG from immune serum with ablastic activity only (previously adsorbed with trypanosomes from immunocompetent hosts to remove the trypanocidal antibodies), but did not adsorb IgG from normal rat serum. To determine whether this specific adsorption of IgG by the parasite could be correlated with a reduction in ablastic activity, immune sera were adsorbed with bloodstream forms from immunosuppressed hosts at packed cell/serum ratios of either 1.2 or 2.0, and the adsorbed sera were then tested for ablastic activity in vitro. With both cell/serum ratios, ablastic activity was reduced by 50%. In comparison, similar adsorptions of immune sera with trypanosomes from immunocompetent hosts resulted in reductions of ablastic activity of only about 9 and 27% with the low and high cell/serum ratios, respectively. It is concluded that the failure to effect significant adsorption of ablastin in earlier studies resulted from the use of ablastinsensitized trypanosomes from immunocompetent hosts. 相似文献
4.
A comparative study has been made of the penetration into leafdisks of Phaseolus vulgaris of (a) phenoxyacetic acid and its2-, 4-, 2, 4-, 2, 6-, 3-5, 2, 4, 5-, and 2, 4, 6- chloro derivatives,and (b) benzoic acid and its 2-, 2, 4-, 2, 5-, 2, 3, 6-chloroand 3, 6-dichloro-2-methoxy derivatives. The methods of synthesisof each compound with 14C incorporated in the carboxyl groupare described. In the series of substituted phenoxyacetic acids it was establishedthat only for 2, 4, 5-T was there an appreciable loss of radioactivityfrom the system either in the light or darkness. In contrast,with the exception of 2, 4-DCBA, 14C is lost from each memberof the series of substituted benzoic acids. The level and pattern of penetration in the two series is differentiallyaffected by chlorination. In general progressive chlorinationof phenoxyacetic acid leads to an increase in the rate of penetrationin both light and darkness. There are, however, exceptions;for illuminated disks the rate of entry of 2,4,5-T is exceededby 2,4,6-T, 2, 4-D, and 2, 6-D whereas in the dark 2,4,5-T hasby far the highest rate of penetration. Progressive chlorinationof benzoic acid, however, depresses entry in both light anddarkness. Possible relationships between these findings and changes inselected physical properties of each series of compounds havebeen examined. Between members of the series of substitutedphenoxyacetic acids there is little variation in the dissociationconstant whereas for the substituted benzoic acids there isa marked lowering of the pK as the degree of chlorination increases.The rate of elution with chloroform of each compound from abuffered Silocel column gives a measure of the degree of partitioninto a lipid medium at a given pH. At pH 5.2 chlorination ofphenoxyacetic acid results in more rapid elution whereas chlorinationof benzoic acid causes a longer hold up. An apparatus was designed to enable measurements to be madeof the rate at which a compound moves from one aqueous phaseto another through a layer of lipid. Chlorination promotes therate of transfer of phenoxyacetic acid through octanol but retardsthe transfer of benzoic acid. The extent to which chlorinationdepresses the rate of transfer of benzoic acid matches the diminutionin the rate of leaf penetration. For the series of substitutedphenoxyacetic acids transfer rates and penetration rates followsimilar trends in the dark but in the light agreement is lessgood. These findings are discussed in relation to prior studies onthe rate of uptake and the metabolic fate of the ndividual compoundsin a number of plant tissues. 相似文献
5.
Two strains of Alcaligenes denitrificans, designated BRI 3010 and BRI 6011, were isolated from polychlorinated biphenyl (PCB)-contaminated soil using 2,5-dichlorobenzoic acid (2,5-DCBA) and 2,4-DCBA, respectively, as sole carbon and energy sources. Both strains degraded 2-chlorobenzoic acid (2-CBA), 2,3-DCBA, and 2,5-DCBA, and were unable to degrade 2,6-DCBA. BRI 6011 alone degraded 2,4-DCBA. Growth of BRI 6011 in yeast extract and 2,6-DCBA induced pyrocatechase activity, but 2,6-DCBA was not degraded, suggesting the importance of an unsubstituted carbon six of the aromatic ring. Metabolism of the chlorinated substrates resulted in the stoichiometric release of chloride, and degradation proceeded by intradiol cleavage of the aromatic ring. Growth of both strains on 2,5-DCBA induced pyrocatechase activities with catechol and chlorocatechols as substrates. In contrast to dichlorobenzoic acids, growth on 2-CBA, benzoic acid, mono- and dihydroxybenzoic acids induced a pyrocatechase activity against catechol only. Although 2,4-DCBA was a more potent inducer of both pyrocatechase activities, its utilization by BRI 6011 was inhibited by 2,5-DCBA. Specific uptake rates using resting cells were highest with 2-CBA, except when the resting cells had been previously grown on 2,5-DCBA, in which case 2,5-DCBA was the preferred substrate. The higher rates of 2,5-DCBA uptake obtained by growth on that substrate, suggested the existence of a separately induced uptake system for 2,5-DCBA. 相似文献
6.
Raman vibrational spectra were obtained from crystalline amino acids and acid molecules adsorbed at the surfaces of silver electrodes in aqueous solutions. Results revealed that aromatic acids such as phenylalanine adsorbed via their aromatic ring while bases such as alanine were coordinated by their amine functional groups. Sulphur containing acids (cysteine and cystine) were found to bond through their sulphur groups. In all cases, adsorption increased towards the point of zero charge of silver, as would be expected for uncharged species. Similar experiments carried out on α‐amylase solutions showed that the enzyme molecule changes its coordination to the silver surface as a function of electrode potential, indicating that C‐S, C‐N, and aromatic ring functional groups are all present on the outer surface of the enzyme structure. 相似文献
7.
Studies on plant growth-regulating substances XXV. The plant growth-regulating activity of cinnamic acids 总被引:2,自引:0,他引:2
Effects of ring substitution on the plant growth-regulating activities of trans- and cis-cinnamic acids have been investigated in the wheat cylinder, pea segment and pea curvature tests. Most of the cis- acids were shown to be active. Substitution of fluorine, chlorine or bromine into the ring of cis-cinnamic acid in most cases increased the activity. The results are discussed in relation to mode of action and chemical structure/biological activity relationships: 4-chlorobenzoic acid is shown to act as a competitive antagonist towards 4-chloro-cis-cinnamic acid. 相似文献
8.
The interrelationships between chemical structure and patternsof uptake by Lemna minor have been examined for (a) phenoxyaceticacid and its 2-, 4-, 2,4-, 2,6-, 3,5-, 2,5-, 2, 4, 5- and 2,4,6-chloroderivatives and (b) benzoic acid and its 2-, 2,4-, 2,5-, 2,3,6-chloro-and 3,6-dichloro-2-methoxy derivatives. All compounds contained14C in the carboxyl group. The plants from a clonal populationwere grown at a constant temperature and continuously illuminated. With progressive chlorination of phenoxyacetic acid, uptakeis enhanced, so that by 6 h there is a fourfold difference betweenthe monochloro- and trichloro-derivatives. In complete contrast,chlorination of benzoic acid greatly suppresses uptake and thedifferences associated with the degree of chlorination are smaller. Arising out of previous studies, the effects of adding streptomycin,synthalin, and cetyltri-methylammoniumbromide on the courseof uptake of individual members of the two series have beenexamined. Each of the additions can induce positive, negative,or null changes in the pattern of uptake, but the nature ofthe response is also dependent on the properties of the compound. These findings are discussed in relation to prior studies concerning(a) penetration into the leaves of Phaseolus vulgaris, (b) uptakeby excised segments of etiolated stems, and (c) changes in physico-chemicalproperties resulting from progressive chlorination. Many of the complexities still remain to be resolved but itseems clear that adsorption by Borne membrane system involvingthe carboxyl group of the entering acid and the positively chargedquaternary ammonium group of alpha-lecithin cannot be restrictedto compounds which are physiologically active as auxins or herbicides. 相似文献
9.
The plant growth-regulating activity of all the mono- and di- chloro-substituted phenylacetic acids, together with the 2:3:6-trichloro- derivative, has been determined in the wheat cylinder, pea segment and pea curvature tests. Each of these compounds showed activity, the 2:3-dichloro- and the 2:3:6-trichloro- derivatives being extremely active. The 3:5-dichloro- acid was inactive in the wheat test but showed moderate activity in the pea tests.
All these findings are discussed in relation to the plant growth-regulating activity of corresponding benzoic and phenoxyacetic acids. 相似文献
All these findings are discussed in relation to the plant growth-regulating activity of corresponding benzoic and phenoxyacetic acids. 相似文献
10.
Specificity of Auxin-binding Sites on Maize Coleoptile Membranes as Possible Receptor Sites for Auxin Action 总被引:20,自引:12,他引:8
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Ray PM 《Plant physiology》1977,60(4):585-591
Dissociation coefficients of auxin-binding sites on maize (Zea mays L.) coleoptile membranes were measured, for 48 auxins and related ring compounds, by competitive displacement of 14C-naphthaleneacetic acid from the binding sites. The sites bind with high affinity several ring compounds with acidic side chains 2 to 4 carbons long, and much more weakly bind neutral ring compounds and phenols related to these active acids, most phenoxyalkylcarboxylic acids, and arylcarboxylic acids except benzoic acid, which scarcely binds, and triiodobenzoic acids, which bind strongly. Specificity of the binding is narrowed in the presence of a low molecular weight “supernatant factor” that occurs in maize and other tissues. Activity of many of the analogs as auxin agonists or antagonists in the cell elongation response was determined with maize coleoptiles. These activities on the whole roughly parallel the affinities of the binding sites for the same compounds, especially affinities measured in the presence of supernatant factor, but there are some quantitative discrepancies, especially among phenoxyalkylcarboxylic acids. In view of several factors that can cause receptor affinity and biological activity values to diverge quantitatively among analogs, the findings appear to support the presumption that the auxin-binding sites may be receptors for auxin action. 相似文献
11.
Mono-oxygenase-like activity occurs in Rhizoctonia solani during the metabolism of phenyl-acetic acid. The partially purified enzyme catalysed only the hydroxylation of phenylacetic acid at its meta position and required NADH and tetrahydrofolate as co-factors. Benzoic, phenylpropionic, trans-cinnamic, and phenoxyacetic acids were not suitable substrates for the enzyme. 相似文献
12.
An examination has been made of the phase of continuous accumulationof phenoxyacetic acid (POA) and the 2-, 4-, 2,4-, 2,6-, and2,4,5-chloro-derivatives, containing carbon-14 in the carboxylgroup, by segments of the Avena mesocotyl. On the basis of previousfindings to eliminate the initial transient components of uptake(Type I processes) the segments were pretreated for 13 to 18h in buffer or buffer containing the respective non-radioactivecompound. For five of the compounds the relationship between the rateof uptake and the external concentration takes the form of arectangular hyperbola, but for the sixth, 2,4,5-T, this relationshipdoes not hold. The data, except those for 2,4,5-T, have beenevaluated as linear regressions of rate of uptake against thequotient of rate over concentration. From each regression equationtwo constants have been derived: the point B wherethe line intersects the rate axis (the theoretically maximumrate) and the slope of the regression K, whichcan alternatively be expressed as the concentration at whichthe observed rate equals half the value of B. The calculated values of B and K for POA are approximately twiceas great as the corresponding values for 2-CPA, and about 25times greater than for 4-CPA. The values for 2,4-D are closeto those for 4-CPA, and 2,6-D is intermediate between 2-CPAand 4-CPA. Although the constants for 2,4,5-T could not be calculatedprecisely, the rates of accumulation are about one-fourth ofthose measured for 2,4-D at equivalent concentrations. The uptake of radioactive 2,4-D is slightly depressed in thepresence of nonradioactive POA. Greater reductions are causedby 2-CPA or 2,6-D, and 2,4-D or 2,4,5-T are even more inhibitory.The pattern of inhibition caused by 2,4,5-T indicates competitionfor common sites of uptake, while POA appears not to be competitive.In corresponding experiments with POA, the presence of the otherregulators only caused small inhibitions and the order was different. Earlier work showed that in Avena accumulation is accompaniedby the conversion to a varying degree of the individual substitutedphenoxyacetic acids to conjugated derivatives. It is suggestedthat the variation between compounds in their rates of accumulationis in part due to differences in the stability of the conjugatedderivatives, and that the facility of conversion is a factorin determining physiological activity. 相似文献
13.
A study has been made of the growth-regulating activity of a number of phenoxy acids possessing halogen or methyl substituents in the 2:6- or 33- positions of the nucleus. The pea curvature, Avena cylinder elongation and tomato-leaf epinasty tests have been used for assessing biological activity. The results show that in general 3:s- is more closely associated with inactivity than 2:6- substitution. The introduction of a further halogen atom into the 4- position does not enhance activity in the 2:6- compounds but does so in the 3:s- derivatives. Although the 2:4:6-trichloro- and 2:4:6-tribromo-phenoxyacetic acids are almost inactive, a:4-dichloro-6-fluoro- and 2:4-dibromo-6-fluoro- phenoxyacetic acids are very highly active. When a methyl group is substituted into the side-chain of certain phenoxyacetic acids possessing 2:6- substituents, growth-promoting activity is increased. Some of these findings conflict with recent theories on mode of action of phenoxy acids which are discussed in relation to the present work. 相似文献
14.
Cristian C. Brunchi Pablo Englebienne Herman J.M. Kramer Sondre K. Schnell 《Molecular simulation》2015,41(15):1234-1244
Despite the wide use of the real adsorbed solution theory to predict multicomponent adsorption equilibrium, the models used for the adsorbed phase activity coefficients are usually borrowed from the gas–liquid phase equilibria. In this work, the accuracy of the Wilson and NRTL models for evaluating adsorbed phase activity coefficients is tested using a 2D-lattice model. An accurate model for adsorbed-phase activity coefficients should have no problem in fitting adsorption data obtained using this simple lattice model. The results, however, show that the commonly used Wilson and NRTL models cannot describe the adsorbed phase activity coefficients for slightly non-ideal to strong non-ideal mixtures. Therefore, until new models for adsorbed phase activity coefficients are developed, we should use existing models for liquids with care. In the second part of this work, the use of Monte Carlo simulations on a segregated 2D-lattice model, for predicting adsorption of mixtures is investigated. The segregated model assumes that the competition for adsorption occurs at isolated adsorption sites, and that the molecules from each adsorption site interact with the bulk phase independently. Two binary mixtures in two adsorbent materials were used as case studies for testing the predictions of the segregated 2D-lattice model: the binary system CO2–N2 in the hypothetical pure silica zeolite PCOD8200029, with isolated adsorption sites and normal preference for adsorption, and the binary system CO2–C3H8 in pure silica mordenite (MOR), with isolated adsorption sites and inverse site preference. The segregated 2D-lattice model provides accurate predictions for the system CO2–N2 in PCOD8200029 but fails in predicting the adsorption behaviour of CO2–C3H8 in pure silica MOR. The predictions of the segregated ideal adsorbed solution theory model are superior to those of the 2D-lattice model. 相似文献
15.
MARY B. PYBUS MARGARET S. SMITH R. L. WAIN F. WIGHTMAN 《The Annals of applied biology》1959,47(1):173-181
The physiological activity of complete series of mono- and di-substituted chloro-and methyl-phenoxyacetic and benzoic acids have been investigated using the wheat cylinder, pea segment and pea curvature tests. In the phenoxyacetic acids, high activity was associated with substitution in the 3-, 4-, 2:4-, 2:5- and 3:4-positions and in general, chlorine had a greater effect than methyl in conferring activity.
With the benzoic acids, 2:3-, 2:5- and 2:6-, disubstitution gave active compounds, the chloro-derivatives again being the more active. The 2:5-compound was the most active in the dichloro- series, all members of which were less active than the 2:3:6-trichloro- and 2:3:5:6-tetrachloro-acids. Benzoic acids substituted in the 4- position were either inactive or exhibited only weak growth-promoting activity.
The results are discussed in relation to recent theories which attempt to relate growth-promoting activity with the position of substituents in the aromatic ring. 相似文献
With the benzoic acids, 2:3-, 2:5- and 2:6-, disubstitution gave active compounds, the chloro-derivatives again being the more active. The 2:5-compound was the most active in the dichloro- series, all members of which were less active than the 2:3:6-trichloro- and 2:3:5:6-tetrachloro-acids. Benzoic acids substituted in the 4- position were either inactive or exhibited only weak growth-promoting activity.
The results are discussed in relation to recent theories which attempt to relate growth-promoting activity with the position of substituents in the aromatic ring. 相似文献
16.
The behavior of lipase produced by Pseudomans mephitica var. lipopytica toward hydrophobic residues coupled to spacer gels that were prepared by coupling a primary amine to CNBr-activated agarose, was studied. The lipase adsorbed on the ligand of a long unbranched aliphatic chain, a benzene ring, or deoxycholic acid was only slightly or not all eluted at pH 5 or pH 11 by buffers containing 1 M NcC1. The lipase was eluted by liquid containing a surfactant or an organic solvent miscible with water, indicating greater involvement of hydrophobic forces. The adsorption of propane, cyclopentane, cyclohexane, cycloheptane, or chrysene appears to be achieved through electrostatic forces, inasmuch as desorption was caused by buffer containing 1 M NaC1 at pH 11. The amount of lipase adsorbed on these hydrophobic ligands was about the same as that adsorbed on the ligands belonging to the first group. Since little lipase wad adsorbed on cyclopropane, cycloctane, pyridine, methane, n-pentane, or branched aliphatic chains, these ligands appear to impose steric hindrance on the adsorption of lipase, or they may be too small to fit into the hydrophobic sites of lipase. 相似文献
17.
Basic solutions of beef extract and casein were able to elute poliovirus adsorbed to four membrane filters with different chemical compositions. Hydrolyzed protein and individual amino acids were able to elute virus adsorbed to certain filters but were unable to elute virus adsorbed to other filters efficiently. A solution of 4 M urea buffered at pH 9 with 0.05 M lysine was able to elute greater than 60% of the virus adsorbed to each of the filters tested. Certain solutions of amino acids were capable of eluting virus adsorbed to one filter but permitted adsorption of virus to another filter with a different chemical composition. Acidic amino acids could interfere with elution of virus from membrane filters. Aromatic compounds with an amino group attached to the ring were good eluents for virus adsorbed to epoxy-fiberglass membrane filters. In contrast, aromatic compounds with other substituents were generally poor eluents. 相似文献
18.
《Journal of receptor and signal transduction research》2013,33(1-4):205-223
AbstractThe unsaturated fatty acids oleic, linoleic and arachidonic inhibited binding of ligands to the ouabain, opiate, and β-adrenergic plasma membrane receptors. Low concentrations of fatty acids slightly increased the binding of ouabain to its binding sites. The effect of these fatty acids on β-adrenergic sensitive adenylate cyclase was more complex. 0.2–0.3 mM fatty acids increased adenylate cyclase activity, while higher concentrations of arachidonic and linoleic acids, but not oleic acid 相似文献
19.
Mella Paecht-Horowitz Frederick R. Eirich 《Origins of life and evolution of the biosphere》1988,18(4):359-387
We studied the spontaneous polymerization of amino acid adenylates on Na-montmorillonite in dilute, neutral suspension, after polypeptides were adsorbed on the clay. This led to the unexpected finding that the degrees of polymerization (DP's) of the oligo- and poly-peptides obtained depended on the amounts of polypeptides that were preadsorbed. Plotting average molecular weights obtained against c-spacings of the clay platelet aggregates which widened as a result of polypeptide addition and adsorption before the polymerization, does not permit an obvious explanation of these observations. The best correlation assigns a role to the fractional occupation of the individual intercalation layers of the polypeptides, as the adsorption increases towards a first complete mono-interlayer, then to an incipient and eventually to a complete double layer on to a third interlayer, after which the clay stacking breaks up. Spacings which correspond to an intermediate occupation of any of the three successive interlayers favor amino acids self-addition to polymers. The opposite is true for nearly empty or filled intercalation layers. We hypothesize and describe, how a catalytic activity may derive from c-spacings that offer adsorption sites for the reagent amino acid adenylate within the peripheral recesses of irregularly stacked clay platelets by bringing the anhydride bonds and neutral amino groups into favorable reaction distances. Moderately filled intercalation spaces may also act as sinks for the newly formed oligomers and facilitate the freeing of reaction sites for the occupation by fresh reagent. The c-spacings required for these mechanisms are the result of the intercalation of the preadsorbed polymer, but similar conditions prevail when polymers are adsorbed as they are generated during polymerization. 相似文献
20.
Effect of Metal Cations and pH on the Antibacterial Activity and Uptake of Long Chain Fatty Acids 总被引:3,自引:3,他引:0
S ummary . The bactericidal activity of long chain saturated fatty acids was antagonized by alkaline earth metals. The activity of linoleic acid was less effectively antagonized but was more sensitive to reversal by ferric and stannous ions. With increasing pH value the bactericidal activity of lauric acid decreased but that of the longer chain saturated acids increased. Both Gram positive and Gram negative bacteria reversibly adsorbed fatty acids. Uptake increased with decreasing pH value and increasing chain length. Although adsorbed to a lesser extent, the intrinsic activity of linoleic acid was greater than lauric acid. The uptake appeared to be non-specific and governed by the physicochemical properties of both the acids and the bacterial cell surfaces. Sensitivity to the fatty acids increased with decreasing pH value. Protoplasts of Bacillus megaterium adsorbed fatty acids to a greater extent than whole cells. Resistance of the Gram negative Pseudomonas phaseolicola was not due to non-adsorption of the fatty acids. 相似文献