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Yang Peng Sanath Kumar Ricardo L. Hernandez Suzanna E. Jones Kathleen M. Cadle Kenneth P. Smith Manuel F. Varela 《The Journal of membrane biology》2009,228(2):79-88
The purpose of this study was to examine the sugar recognition and transport properties of the sucrose permease (CscB), a
secondary active transporter from Escherichia coli. We tested the hypothesis that maltose transport is conferred by the wild-type CscB transporter. Cells of E. coli HS4006 harboring pSP72/cscB were red on maltose MacConkey agar indicator plates. We were able to measure “downhill” maltose transport and establish definitive
kinetic behavior for maltose entry in such cells. Maltose was an effective competitor of sucrose transport in cells with CscB,
suggesting that the respective maltose and sucrose binding sites and translocation pathways through the CscB channel overlap.
Accumulation (“uphill” transport) of maltose by cells with CscB was profound, demonstrating active transport of maltose by
CscB. Sequencing of cscB encoded on plasmid pSP72/cscB used in cells for transport studies indicate an unaltered primary CscB structure, ruling out the possibility that mutation
conferred maltose transport by CscB. We conclude that maltose is a bona fide substrate for the sucrose permease of E. coli. Thus, future studies of sugar binding, transport, and permease structure should consider maltose, as well as sucrose.
Yang Peng and Sanath Kumar contributed equally to this paper. 相似文献