共查询到20条相似文献,搜索用时 31 毫秒
1.
Characterization of a pollen-specific cDNA clone from Nicotiana tabacum expressed during microgametogenesis and germination 总被引:5,自引:0,他引:5
Koen Weterings Wim Reijnen Roel van Aarssen Anne Kortstee John Spijkers Marinus van Herpen Jan Schrauwen George Wullems 《Plant molecular biology》1992,18(6):1101-1111
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de Groot P Weterings K de Been M Wittink F Hulzink R Custers J van Herpen M Wullems G 《Plant molecular biology》2004,55(5):715-726
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F. R. A. Wittink B. Knuiman J. Derksen V. Čapková D. Twell J. A. M. Schrauwen George J. Wullems 《Sexual plant reproduction》2000,12(5):276-284
The pollen-specific gene Ntp303 belongs to the class of late pollen specific genes. It is first transcribed directly after pollen mitosis. Biochemical properties,
appearance and precise location of the NTP303 protein during pollen development and pollen tube growth were studied by amino-acid
micro-sequencing, protein gel blotting and immuno-localization. Antisera were raised against recombinant proteins, encoded
by sequences of the pollen-specific Ntp303 gene. The antibodies specifically recognized a 69-kDa glycoprotein. Electron-microscopic immuno-localization of the protein
revealed the presence of high concentrations of the NTP303 protein at the vegetative plasma membranes that surround the vegetative
cell, the generative cell and the sperm cells of pollen and pollen tubes. The generative plasma membranes of the generative
cell and the sperm cells were negative. NTP303 protein was also present in the cell walls and in callose plugs. With this
method it was shown that the NTP303 protein was already present in mid-bicellular pollen, after the first, asymmetrical pollen
mitosis. Possible functions for the NTP303 protein are discussed in relation to its properties and its association with the
vegetative plasma membranes.
Received: 9 September 1999 / Revision accepted: 4 November 1999 相似文献
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Regulation of flavonol biosynthesis during anther and pistil development, and during pollen tube growth in Solanum tuberosum 总被引:1,自引:0,他引:1
Gerben J. van Eldik Wim H. Reijnen René K. Ruiter Marinus M.A. van Herpen Jan A.M. Schrauwen George J. Wullems 《The Plant journal : for cell and molecular biology》1997,11(1):105-113
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Functional dissection of the promoter of the pollen-specific gene NTP303 reveals a novel pollen-specific, and conserved cis-regulatory element 总被引:7,自引:0,他引:7
Koen Weterings Jan Schrauwen George Wullems David Twell 《The Plant journal : for cell and molecular biology》1995,8(1):55-63
Regulatory elements within the promoter of the pollen-specific NTP303 gene from tobacco were analysed by transient and stable expression analyses. Analysis of precisely targeted mutations showed that the NTP303 promoter is not regulated by any of the previously described pollen-specific cis -regulatory elements. However, two adjacent regions from −103 to −86 bp and from −86 to −59 bp were shown to contain sequences which positively regulated the NTP303 promoter. Both of these regions were capable of driving pollen-specific expression from a heterologous promoter, independent of orientation and in an additive manner. The boundaries of the minimal, functional NTP303 promoter were determined to lie within the region −86 to −51 bp. The sequence AAATGA localized from −94 to −89 bp was identified as a novel cis -acting element, of which the TGA triplet was shown to comprise an active part. This element was shown to be completely conserved in the similarly regulated promoter of the Bp10 gene from Brassica napus encoding a homologue of the NTP303 gene. 相似文献
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Isolation and partial characterization of a novel pollen-specific cDNA with multiple polyadenylation sites from wheat 总被引:3,自引:0,他引:3
Wheat is the foremost staple food crop that offers bothcalories and proteins to a large global population. Wheat(hexaploid AABBDD genome, 16 billion bp) is a geneti-cally complex, self-pollinating plant with bisexualflowers that produce short-lived pollen. Very little is knownabout the molecular biology of its gametophyte develop-ment despite a longstanding interest in hybrid seeds. Mostof our information is from the studies on a few model andcrop plants such as Arabidopsis, tobacco, vegeta… 相似文献
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Tania A. Plyushch M. T. M. Willemse M. A. W. Franssen-Verheijen M. C. Reinders 《Protoplasma》1995,187(1-4):13-21
Summary In vitro penetration of the micropyle of freshly isolatedGasteria verrucosa ovules by pollen tube was monitored on agar medium. 40–60% of the micropyles were penetrated, comparable with in vivo penetration percentages. When germinated on agar,Gasteria pollen tube elongation lasts for up to 8 h while plasma streaming continues for about 20–24 h. The generative cell divides between 7 and 20 h after germination, and after 20 h the pollen tube arrives at one of the synergids. The sperm cells arrive after 22 h. The whole process takes more time in vitro than in vivo. In fast growing pollen tubes, a pulsed telescope-like growth pattern of tube elongation is observed. The formation of pollen tube wall material precedes tube elongation and probably prevents regular enlargement of the pollen tube tip-zone. Rapid stretching of the new pollen tube wall material follows, probably due to gradually increased osmotic pressure and the use of lateral wall material below the tip. The stretching ceases when the supplies of plasma membrane and excretable wall material are exhausted. Multiple pollen tube penetration of the micropyle occurs in vitro as it does in vivo. Most pollen tube growth ceases within the micropyle but, if it continues, the pollen tubes curl. Inside the micropyle the pollen tube shows haustorial growth. At the ultrastructural level, the wall thickening of in vitro pollen tubes is quite similar to that in vivo. Before transfer of pollen tube cytoplasm a small tube penetrates one of the synergids. Sperm nuclei with condensed chromatin are observed in the pollen tube and the synergid. In vivo prometaphase nuclei are found in the most chalazal part of a synergid, against the egg cell nucleus and nucleus of the central cell at a later stage. Using media forLilium ovule culture,Gasteria ovules were kept alive for at least 6 weeks. Swelling of the ovule depends on pollen tube penetration. The conditions for fertilization to occur after in vitro ovular pollination seem to be present. 相似文献
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Reversible protein tyrosine phosphorylation affects pollen germination and pollen tube growth via the actin cytoskeleton 总被引:1,自引:0,他引:1
Summary. Phenylarsine oxide (PAO) and genistein are two well-known specific inhibitors of tyrosine phosphatases and kinases, respectively,
that have been used in the functional analysis of the status of protein phosphotyrosine in different cell types. Our experiments
showed that both PAO and genistein arrested pollen germination and pollen tube growth and led to the malformation of the pollen
tubes, although genistein had a lesser effect. The malformations of the pollen tubes caused by PAO and genistein were, however,
quite different. In addition, it was found that the rate of pollen germination and tube growth recovered to a certain extent
when phalloidin was present during PAO treatment, but not when it was present during genistein treatment. Furthermore, PAO
treatment also had a great effect on the dynamic organization of filamentous actin in the pollen grain and pollen tube, while
genistein only caused reorganization of actin at the turning point of the pollen tube. Our results suggest that reversible
protein tyrosine phosphorylation is a crucial step in pollen germination and pollen tube growth, but that tyrosine kinases
and phosphatases may have different effects which may function through the reorganization of the actin cytoskeleton.
Correspondence and reprints: Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education, College
of Life Science, Beijing Normal University, Beijing 100875, People’s Republic of China. 相似文献
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From a pollen tube cDNA library ofPetunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing thePPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in thePPE1 gene. During pollen development,PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level inin vitro germinated pollen tubes. The observed expression pattern of thePPE1 gene suggests that its product may play a role in pollen germination and/or tube growth. 相似文献
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Helin Tan Jiahuan Zhang Xiao Qi Wenxue Ye Xingchun Wang Xiaoe Xiang 《Plant molecular biology》2018,97(4-5):325-335