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1.
In debladed bean petioles calcium and dry weight increased in the abscission zone during an induction period of 14 hr. Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone and increased in the petiole. Dry matter began to decrease in both the abscission zone and the petiole 24 hr after deblading. The first visual change in the cells of the abscission zone was a swelling of the pectic materials of the cell walls. This was followed by breakdown of other cell wall components, i.e., non-cellulosic polysaccharides and cellulose. The cellulose of the cell walls adjacent and distal to the abscission layer was found to be altered; however, no lignin was present during abscission layer development. The alteration of pectic materials, coupled with breakdown of cell wall components, resulted in the collapse of cells of the abscission layer just prior to separation. Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight. 相似文献
2.
The Anatomy of Fruit Abscission in Loganberries 总被引:1,自引:0,他引:1
Loganberry fruits abscise at the base of the receptacle, justdistal to the sepals. As the fruit ripens, all cells of theabscission zone expand. The central parenchyma cells, due totheir position, appear to be the driving force behind abscission.Their expansion causes early cell-separation within a superficialzone of small cells and rupture of the epidermis at the sepal/receptaclejunction without prior dissolution of cell walls. However, othercells within the abscission zone have their walls degraded,mostly in the region of the middle lamella, as ripening progresses. Xylem transfer cells are found in abundance in the vascularbundles supplying the sepals. The outward curve of these bundlesinto the sepals brings the transfer cells into close proximitywith the abscission zone. A comparison of their distributionin loganberries with that in raspberries (MacKenzie, 1979),which are closely related but abscise at a different site, suggeststhat transfer cells may be implicated in the abscission process. The inevitable structural weakness brought about by the paucityof vascular tissue in the abscission zone relative to the morerobust pedicel may also predispose this area to separation. Anatomy, abscission, loganberry, Rubus idaeus x R, ursinus, Mailing Sunberry, transfer cells structure, fruit 相似文献
3.
The basic anatomy of lateral twig insertion onto the main branch in both healthy and damaged Quercus cerris L. trees was studied. An abscission zone is always present: in healthy trees it is formed by a smaller number of cell layers than in damaged ones, where it is more evident with many layers of cells. Cells of the abscission zone are roundish, with many intercellular spaces between them; cell walls are thin, non-lignified and without secondary walls. No starch was found in cells of the abscission zone, where, instead, a few scattered calcium oxalate druses are seen. 相似文献
4.
Summary Abscission layer formation in the sour cherry (Prunus cerasus L.) during fruit maturation occurred in the transition zone between the fruit and the pedicel. The abscission layer, consisting of 5–8 rows of cells, was first identified by its low affinity for haematoxylin. The walls of cells in the abscission layer contained less total polysaccharides than adjacent cells. The pectins were degraded and the cellulose was partially broken down resulting in cell separation. The Ca level in the abscission zone decreased and Ca and Mg were lost from the walls of cells in the layer during abscission. After the abscission layer formed, cells associated with the layer had a lower capacity to bind 45Ca than cells distal or proximal to the layer.Michigan Agricultural Experiment Station Journal Article No. 4607 相似文献
5.
Barbara D. Webster 《American journal of botany》1973,60(5):436-447
This paper reports light and electron microscope observations of changes in the walls of cortical cells in the laminar abscission region of red kidney bean (Phaseolus vulgaris L.). In intact plants two or three rows of cells comprise the abscission zone. Pectic substances are not present in the walls of these cells when wall breaks occur. The separation cavity involves breaks in both radial and longitudinal cell walls. In ethylene-treated explants pectic substances are present in the cell walls when breaking occurs. The separation cavity involves breaks in longitudinal walls only, and breaking is confined to a single row of cortical cells. Prior to cell wall break the plasma membrane frequently invaginates. In intact plants this may be associated with plasmolysis and with the formation of secondary vacuoles. In ethylene-treated explants it may also be related to plasmolysis. At the time of cell wall break many unidentifiable inclusions of varying sizes and shapes are present in the cell wall region. Chloroplasts and mitochondria are structurally altered but recognizable in the cell at the time of wall break. Plasmodesmata are frequently observed in abscission cells and may be structurally elaborate. The observations of the nature of cell wall changes during abscission in ethylene-treated material fail to confirm physiological studies of other workers suggesting that pectin dissolution is necessary and may be sufficient for formation of a separation layer. 相似文献
6.
R. Sexton 《Protoplasma》1979,99(1-2):53-66
Summary The abscission of leaves fromImpatiens sultani Hook. occurs as the direct result of the weakening of a narrow band of cells running transversely across the base of the petiole. This loss of strength of the abscission zone is due to the breakdown of the central cell wall in two or three layers of cells. The process of wall degeneration is first visible 13 hours after the induction of abscission in a small group of cells found just below the concave groove on the adaxial side of the petiole. As the abscission zone gets progressively weaker the area of cells showing wall breakdown expands, spreading through the parenchyma to the lower side of the stele. The walls of the collenchyma and epidermis along the sides and base of the petiole and the central vascular tissues are the last to break down. Experiments in which the abscission zone was dissected into small pieces were undertaken to investigate whether cell wall hydrolysis was a contagious phenomenon, spreading from cell to cell by direct contact. The results of these investigations indicated that there was little requirement for cell to cell contact in either the temporal or spatial integration of cell wall breakdown. 相似文献
7.
R. Stösser 《Planta》1970,90(3):299-302
Summary 2-Chloroethylphosphonic acid (CEPA) facilitates the separation of the fruit from the pedicel significantly. The application of 2,000 and 4,000 ppm CEPA in four sweet cherry varieties during maturation resulted in the formation of a complete abscission layer in the transition zone between pedicel and fruit. In contrast, in the untreated fruit no abscission layer was evident at maturity. The walls of the cells in the abscission layer contained less total polysaccharides than adjacent cells. Cellulose was partially broken down, and the pectins were degraded. The Ca and Mg content in the cell walls decreased. Thus the same histochemical changes are involved in natural and CEPA induced abscission. 相似文献
8.
The anatomy, histochemistry, kinetics and hormonal regulationof perianth abscission in Crocosmia x crocosmiiflora (Montbretia)has been investigated. The abscission zone is anatomically welldefined, with cell divisions occurring in this region at anthesis.Abscission is first detectable 3 d after perianth opening, whenthe walls of a group of cells beneath the adaxial epidermisshow reduced staining with polyanion-specific stains, and adecline in penanth break strength also occurs. Abscission isachieved by cell wall breakage in thc abscission zone, whichprogresses eccentrically from the adaxial epidermis throughthe abscission zone, rather than the separation of intact cellsas occurs in flowers of dicotyledons. Experiments on detachedflowers suggest similarities in the hormonal regulation of abscissionin Crocosmia to that of dicotyledons, in that an ethylene promotion,and possibly an auxin inhibition, mechanism may exist in Crocosmia.Ovary expansion occurs throughout the development and senescenceof unpollinated flowers, but does not appear to be the solecause of wall breakage in the abscission zone. It is suggestedthat hormonally regulated wall hydrolases weaken the cell wallsin the abscission zone, and allow wall breakage and subsequentabscission to occur. Cdrocosmia x crocosmiiflora, Montbretia, anatomy, breakstrength, cell wall changes, histochemistry, flowers, monocotyledons, perianth, senescence, ethylene, auxin 相似文献
9.
Treatment with ethylene accelerated the abscission of branches of Azolla filiculoides plants. An Azolla plantlet treated with ethylene at 10 microl liter(-1) divided into 4-5 fragments after a lag period of 6-8 h. Ethylene-induced abscission was effectively inhibited by cycloheximide and was associated with an increase in the activities of cellulase and polygalacturonase. At the fracture surface abscised after treatment with ethylene, dissolution of the primary walls of the abscission zone cells was apparent. However, the middle lamella between abscission zone cells was still present. Immunoelectron microscopy using anti-unesterified pectin (JIM5) and anti-methylesterified pectin (JIM7) monoclonal antibodies revealed the presence of both JIM5 and JIM7 epitopes in the wall between abscission zone cells of branches before abscission occurred. In the middle lamella remaining after ethylene-induced abscission, only JIM7 epitopes were observed. The features of ethylene-induced abscission described herein were different from those of the rapid abscission induced by sodium azide, which implies that they are mediated by different mechanisms. The possible mechanisms are discussed. 相似文献
10.
6-benzylaminopurine (BAP) delays leaf abscission of soybeanGlycine max (L.) Merr. Abscission of the distal pulvinus ofprimary leaves was induced in 12-d-old seedlings or explantsby removal of the leaf blade. BAP applied to the cut end ofthe pulvinus following leaf blade removal delayed abscission.Discoloration of the pulvinus occurred before abscission commencedand the number of grana in chloroplasts within cortical parenchymacells of the pulvinus decreased over time following leaf bladeremoval. BAP prevented discoloration of pulvinus tissues anda decrease in grana number. Starch grains within amyloplastsof cells of the starch sheath in the pulvinus disappeared followingleaf blade removal, whereas starch accumulated within the abscissionzone prior to abscission. BAP prevented this apparent redistributionof starch and instead promoted an increase in starch withinplastids of cortical parenchyma cells of the pulvinus. Duringthe abscission process, cells within the separation layer enlargedand their nuclei and nucleoli became more evident prior to theirseparation from one another. Cell separation resulted from breakdownof middle lamellae and partial degradation of primary cell walls.Cycloheximide applied directly to the external surface of theabscission zone inhibited abscission in a similar way to theBAP treatment. These results suggest that BAP prevents abscissionby altering patterns of starch distribution in the pulvinusand abscission zone and by inhibiting the synthesis of proteinsthat typically appear de novo in induced abscission zone tissues. Key words: Benzylaminopurine, BAP, Soybean, Pulvinus, Abscission, amyloplast. 相似文献
11.
Summary The fine structure of the abscission zones of Lycopersicon esculentum and Nicotiana tabacum flower pedicels was studied, with special reference to structural changes in the walls of cells during the abscission process. The separation of cells appeared to be initiated primarily in the middle-lamella region of the cell walls. Disintegration of the primary wall, which usually followed breakdown of the middlelamella region, also occurred concurrently with the lysis of the middle-lamella region. During cell-wall degradation, the walls appeared to swell and became highly flexible. The walls of at least some cells in the zone of separation invaginated during the advanced stages of cell-wall disintegration, and ultimately collapsed. Cell-wall changes in abscising pedicels are almost identical to those which occur in abscising cotton and Coleus leaves, as described by Bornman (1967). 相似文献
12.
When viewed under the light microscope, the abscission zoneat the petal base of Pelargonium x hortorum consisted of smallcells which, when stained with Toluidine Blue, possessed denselystained cells walls. After treatment with 1 µl l-1 ethyleneat 22°C, the force required to separate the petals fromthe receptacle declined after a lag phase of only 30 min, withseparation complete 60-90 min later depending upon the stageof development of the flower. Transmission electron micrographsof the petal abscission zones showed evidence of cell wall degradation,particularly in the middle lamella. These cells also containedextensive rough endoplasmic reticulum and numerous Golgi bodiesribosomes. When abscission was complete, cells at the fractureface showed evidence of breakdown of cellular compartmentalization,often with little sign of an intact tonoplast. Scanning electronmicrographs of recently-abscissed surfaces showed that the epidermalcells surrounding the abscisson zone were turgid and rounded,whereas those of the mesophyll cells were partially collapsed.The micrographic evidence is consistent with the hypothesisthat ethylene-induced separation is caused by rapid enzymaticof the cell walls.Copyright 1993, 1999 Academic Press Abscission, cell walls, ethylene, flower, Pelargonium x hortorum 相似文献
13.
Lee Y Derbyshire P Knox JP Hvoslef-Eide AK 《The Plant journal : for cell and molecular biology》2008,54(6):993-1003
Alterations in the detection of cell wall polysaccharides during an induced abscission event in the pedicel of Euphorbia pulcherrima (poinsettia) have been determined using monoclonal antibodies and Fourier transform infrared (FT-IR) microspectroscopy. Concurrent with the appearance of a morphologically distinct abscission zone (AZ) on day 5 after induction, a reduction in the detection of the LM5 (1→4)-β- d -galactan and LM6 (1→5)-α- l -arabinan epitopes in AZ cell walls was observed. Prior to AZ activation, a loss of the (1→4)-β- d -galactan and (1→5)-α- l -arabinan epitopes was detected in cell walls distal to the AZ, i.e. in the to-be-shed organ. The earliest detected change, on day 2 after induction, was a specific loss of the LM5 (1→4)-β- d -galactan epitope from epidermal cells distal to the region where the AZ would form. Such alteration in the cell walls was an early, pre-AZ activation event. An AZ-associated de-esterification of homogalacturonan (HG) was detected in the AZ and distal area on day 7 after induction. The FT-IR analysis indicated that lignin and xylan were abundant in the AZ and that lower levels of cellulose, arabinose and pectin were present. Xylan and xyloglucan epitopes were detected in the cell walls of both the AZ and also the primary cell walls of the distal region at a late stage of the abscission process, on day 7 after induction. These observations indicate that the induction of an abscission event results in a temporal sequence of cell wall modifications involving the spatially regulated loss, appearance and/or remodelling of distinct sets of cell wall polymers. 相似文献
14.
Anatomical Changes and Immunolocalization of Cellulase during Abscission as Observed on Nitrocellulose Tissue Prints 总被引:1,自引:0,他引:1
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A fundamental event in abscission is the breakdown of cell wall material in a discrete zone of cells known as the separation layer. Three dimensional images produced by viewing tissue prints of abscission zones on nitrocellulose (NC) membranes with incident illumination showed changes in the tissue integrity taking place in the separation layer as the process of abscission proceeded. The cell softening which occurs due to the dissolution of the cell wall appeared in the tissue prints as a diffuse line at the anatomical transition between the pulvinus and petiole and was easily observed on NC tissue prints of either longitudinal or serial cross-sections through abscission zones. In bean leaf abscission the dissolution of cell walls has been correlated with the appearance of a form of cellulase with an isoelectric point of pH 9.5. Antibodies specific for this enzyme were used to study the localization of 9.5 cellulase in the distal abscission zone of Phaseolus vulgaris L., cv Red Kidney after tissue printing on NC. It was found that 9.5 cellulase was localized in the separation layer but also occurred in the vascular tissue of the adjacent pulvinus. No antibody binding was observed in nonabscising tissue or preimmune controls. These results confirm previous biochemical studies and demonstrate that immunostaining of nitrocellulose tissue prints is a fast and reliable method to localize proteins or enzymes in plant tissue. 相似文献
15.
Some ultrastructural observations on the nature of foliar abscission in Impatiens sultani 总被引:1,自引:0,他引:1
Roy Sexton 《Planta》1976,128(1):49-58
Summary Both scanning and transmission electron microscopes have been used to study the anatomy of the abscission zone of Impatiens sultani Hook. Evidence is presented to show that the fracture line follows the middle lamella in all the living cells of the abscission zone including those in the vascular traces. The separation of these cells is preceded by a breakdown of the middle lamellar region of the wall. The characteristics of this process vary in different cell types. Accompanying this breakdown is an enlargement of inner cortex cells mainly in a direction parallel to the axis of the petiole. It is suggested that this expansion of cells is necessary to produce the tensions which rupture the cuticle and xylem vessels prior to separation. The occurrence of transfer cells and tyloses in the abscission zone is also described and the physiological implications of their presence discussed. 相似文献
16.
Further examination of abscission zone cells as ethylene target cells in higher plants 总被引:1,自引:0,他引:1
McManus MT 《Annals of botany》2008,101(2):285-292
BACKGROUND AND AIMS: Two aspects of the competence of abscission zone cells as a specific class of hormone target cell are examined. The first is the competence of these target cells to respond to a remote stele-generated signal, and whether ethylene acts in concert with this signal to initiate abscission of the primary leaf in Phaseolus vulgaris. The second is to extend the concept of dual control of abscission cell competence. Can the concept of developmental memory that is retained by abscission cell of Phaseolus vulgaris post-separation in terms of the inductive/repressive control of beta-1,4-glucan endohydrolase (cellulase) activity exerted by ethylene/auxin be extended to the rachis abscission zone cells of Sambucus nigra? METHODS: Abscission assays were performed using the leaf petiole-pulvinus explants of P. vulgaris with the distal pulvinus stele removed. These (-stele) explants do not separate when treated with ethylene and require a stele-generated signal from the distal pulvinus for separation at the leaf petiole-pulvinis abscission zone. Using these explants, the role of ethylene was examined, using the ethylene action blocker, 1-methyl cyclopropene, as well as the significance of the tissue from which the stele signal originates. Further, leaf rachis abscission explants were excised from the compound leaves of S. nigra, and changes in the activity of cellulase in response to added ethylene and auxin post-separation was examined. KEY RESULTS: The use of (-stele) explants has confirmed that ethylene, with the stele-generated signal, is essential for abscission. Neither ethylene alone nor the stelar signal alone is sufficient. Further, in addition to the leaf pulvinus distal to the abscission zone, mid-rib tissue that is excised from senescent or green mid-rib tissue can also generate a competent stelar signal. Experiments with rachis abscission explants of S. nigra have shown that auxin, when added to cells post-separation can retard cellulase activity, with activity re-established with subsequent ethylene treatment. CONCLUSIONS: The triggers that initiate and regulate the separation process are complex with, in bean leaves at least, the generation of a signal (or signals) from remote tissues, in concert with ethylene, a requisite part of the process. Once evoked, abscission cells maintain a developmental memory such that the induction/repression mediated by ethylene/auxin that is observed prior to separation is also retained by the cells post-separation. 相似文献
17.
Jasmonic acid (JA) and its related compounds (jasmonates) applied to plant tissues exert either inhibitory or promotive effects in growth and developmental processes, which in some ways are similar to abscisic acid. However, little is known about the mode of action of the jamonates at the tissue or organ levels. Here, we review partial evidence for the physiological action of the jasmonates on cell elongation and abscission.
Jasmonates inhibit the IAA-induced cell elongation of oat coleoptile segments not by affecting energy production, osmoregulation and cell wall loosening, but by inhibiting the synthesis of cell wall polysaccharides. The inhibition is partially reversed by simultaneous application of sucrose. Inhibition of IAA-induced elongation by JA is only observed in monocotyledons, not in dicotyledons. These effects suggest that jasmonates exert their inhibitory effect on cell elongation by affecting the metabolism of the cell wall polysaccharides in monocotyledons.
Jasmonates promote the abscission of bean petiole explants without enhancing ethylene production. Cells in the petiole adjacent to the abscission zone expand during abscission. In the abscission zone, jasmonates decrease the amount of cellulosic but not that of noncellulosic polysaccharides. Jasmonates increase the activities of cellulase and decrease the levels of UDP-sugars, which are important intermediates for the synthesis of cell wall polysaccharides in the abscission zone, probably resulting in the decreased level of cellulose and the mechanical weakness of cell walls.
Thus, it is suggested that jasmonates exert their multiple physiological effects by affecting the metabolic processes of cell wall polysaccharides. 相似文献
Jasmonates inhibit the IAA-induced cell elongation of oat coleoptile segments not by affecting energy production, osmoregulation and cell wall loosening, but by inhibiting the synthesis of cell wall polysaccharides. The inhibition is partially reversed by simultaneous application of sucrose. Inhibition of IAA-induced elongation by JA is only observed in monocotyledons, not in dicotyledons. These effects suggest that jasmonates exert their inhibitory effect on cell elongation by affecting the metabolism of the cell wall polysaccharides in monocotyledons.
Jasmonates promote the abscission of bean petiole explants without enhancing ethylene production. Cells in the petiole adjacent to the abscission zone expand during abscission. In the abscission zone, jasmonates decrease the amount of cellulosic but not that of noncellulosic polysaccharides. Jasmonates increase the activities of cellulase and decrease the levels of UDP-sugars, which are important intermediates for the synthesis of cell wall polysaccharides in the abscission zone, probably resulting in the decreased level of cellulose and the mechanical weakness of cell walls.
Thus, it is suggested that jasmonates exert their multiple physiological effects by affecting the metabolic processes of cell wall polysaccharides. 相似文献
18.
Abscission of flowers and floral parts 总被引:21,自引:5,他引:16
The abscission of inflorescences, flowers, petals, sepals, styles,and stamens is discussed, with emphasis on the anatomy and ultrastructureof the abscission zones, and the role of cell wall degradingenzymes and hormonal control. Shedding of these parts is usuallydue to cell wall dissolution, but abscission of petals, stamens,and styles in some species occurs due to the forces generatedby the growing fruit. Flower abscission is clearly regulatedby ethylene, whilst auxins apparently decrease the sensitivityto ethylene. Petal, style and stamen abscission also seems tobe controlled by endogenous ethylene. Auxin is apparently involvedin abscission of styles and stamens, but in petals its roleis at yet unclear. The ultrastructural data indicate high proteinsynthesis and high secretory activity of material toward cellwalls of abscission zone cells. The physiological evidence indicatesa role of both polygalacturonase and cellulase in cell walldissolution, whilst the role of other cell wall degrading enzymesis still unknown. The physiological processes occurring in thewalls of the separating cells should be distinguished from thoserelating to defence against microbial intrusion, such as depositionof lignin and suberin and tylose formation. Experimentationusing mutants and transgenic plants may aid in separating theseprocesses. Sequencing of the isoenzymes specific for the abscissionzone and a search for abscission zone-specific promoters seemsa requirement for the successful evaluation of the enzymes involvedin cell wall degradation. Key words: Abscission, anatomy, abscission zone, hormonal control, cell wall degrading enzymes, inflorescences 相似文献
19.
Auxin and gibberellin effects on cell growth and starch during abscission in cotton 总被引:2,自引:1,他引:1
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An increase in starch content of cells in the abscission zone of the cotton explant appeared correlated with an increase in number of cells. A large increase in the number of cells in the abscission zone, concomitant with an increase in starch content, followed treatment with gibberellin as compared to auxin. In the final stages of abscission starch was hydrolyzed in the cells of the separation layer. Some starch remained after the petiole abscised.
A positive phloroglucinol-hydrochloric acid reaction in the cells of the petiole distal to the line of separation indicated the presence, not of lignin, but of soluble sugars and uronic acids. This reaction was especially intense following gibberellic acid treatment.
It was concluded that gibberellin in accelerating abscission leads to (1) an increase in cell number and starch content in the abscission zone, (2) the hydrolysis of starch in the separation layer just before abscission, and (3) the breakdown of polysaccharides and the release of soluble sugars and uronic acids. Auxin, an abscission retardant, either delays or prevents these events.
相似文献20.
Summary In leaves of S. nigra, fragmentation of the rachis follows the autumnal abscission of leaflets and the high levels of ethylene produced by the senescing blades. Fragmentation is accompanied by cell growth and ultrastructural changes in a zone of cells precisely differentiated at the separation zone. Studies with explants from the rachis show that those that contain an abscission zone increase in freshweight by as much as 50% before and during cell separation. Cell growth changes are induced by ethylene but not by auxin, and are restricted to explants that contain the separation zone cells. In ethylene, enlarging cells of the zone show cytoplasmic activation indicated by dilated dictyosomes, enhanced production of Golgi vesicles, elongated profiles of rough endoplasmic reticulum, a crenellated plasmalemma, and the apparent discharge and accumulation of cytoplasmic vesicles within the desmotubules of the branched plasmodesmata. Degradation of the middle lamella and cell wall matrix could be associated with the release of hydrolytic enzymes on the disruption of the vesicles. Although ultrastructural changes of a similar but limited nature occur in all cells of the rachis in response to ethylene, only those that are morphologically delimited as zone cells exhibit the growth and separation that leads to rachis fragmentation. It is proposed that abscission can occur only at the sites of the positional differentiation of these special ethylene-responsive target cells.Abbreviation IAA
indole-acetic acid (auxin) 相似文献