Thermoluminescence and Delayed Luminescence Investigation of the Green Alga,Chlamydobotrys stellata

Thermoluminescence and delayed luminescence investigations of the autotrophically and photoheterotrophically cultivated green alga, Chlamydobotrys stellata, demonstrated that both the thermoluminescence and delayed luminescence yields are much lower in the photoheterotophic algae than in the autotrophic ones due to an efficient luminescence quenching of unknown mechanism. The relative contributions of the so called Q (S₂Q^?_A charge recombination) and B (S₂Q^?_B and S₃Q^?_B charge recombinations) thermoluminescence bands to the glow curve as well as the Q_A(S₂Q^?_B charge recombination) and Q_B (S₂Q^?_B and S₃Q^?_B charge recombinations) delayed luminescence components to the delayed luminescence decay of autotrophically and photoheterotrophically cultivated Chl. stellata were compared using a computer assisted curve resolution method. It was found that, while in the autotrophic cells the area of the B band was considerably larger than of the Q band, in photoheterotrophic cells the Q band was more effectively charged than the B band. In the delayed luminescence decay curves measured in the seconds to minutes time region the amplitude of the Q_A component relative to that of the Q_B component was larger in the photoheterotrophic cells than in the autotrophic ones. These observations demonstrate that, after light-induced charge separation in the photosystem II reaction centers of autotrophic cells, electrons are “quasipermanently” stored mainly in the secondary quinone acceptor pool, Q_B but in the nonquenched photosystem II reaction centers of photoheterotrophic cells the main reservoir of electrons is the primary quinone acceptor, Q_A. This behaviour indicates an inhibition of electron transport in the photoheterotrophic alga at the level of the secondary quinone acceptor, Q_B.     

The fine structure of some carabid beetle eyes,with particular reference to ciliary structures in the retinula cells

Paired centrioles and associated ciliary root material occur in all eight retinula cells in the nine species investigated. In the diurnal Notiophilus, Elaphrus and Bembidion where the distal rhabdomere of cell 7 is fused with the proximal rhabdom formed by cells 1 to 6, the roots in cells 1 to 6 extend for the entire length of the retinula. In Notiophilus their arrangement around the rhabdom suggests a complementary mechanical relationship between the six large roots and the four Semper cell processes. In five relatively nocturnal species a retinula cell column separates the distal rhabdomere from the proximal rhabdom. In cells 1 to 6 root material is associated with the distally located centrioles as follows. In Leistus roots extend into the proximal rhabdom layer. In Loricera and Agonum roots at the level of the proximal rhabdom are not continuous with the rootlets or short roots associated with the centrioles. In Pseudophonus and Feronia, and in the diurnal Cicindela, short rootlets link the centrioles. Cell movements on dark-adaptation of Notiophilus and Cicindela include shortening of the crystalline tract. In Notiophilus the entire rhabdom is apparently displaced, whereas in Cicindela the narrow distal rhabdomere becomes dissociated from the proximal rhabdom.     

The fine structure of gastric epithelial cells in the suckling rabbit with particular reference to the parietal cell

Summary The gastric parietal cells of newborn rabbits show a reduction in number and change in distribution of the smooth cytoplasmic vesicles when compared with the full term fetus. The apical surface and intracellular canaliculus become more complex. The changes are believed to be a manifestation of the onset of acid secretion. The remaining cells of the mucosa and of the submucosal connective tissue show dilatation of endoplasmic reticulum and accumulation of fat with other abnormal features of non-specific nature and unknown origin.     

Observations on the transparency of the waters of the Pulicat Lake with particular reference to plankton production

Marked sectoral and seasonal variations were observed in the transparency of the waters of the Pulicat lake.No tidal effect was noticed on the turbidity of the waters.The transparency of the waters in relation to the production of plankton has been discussed.     

Observations on the distribution of anophelines in Suriname with particular reference to the malaria vector Anopheles darlingi

A study was made on the distribution of anophelines in Suriname with special emphasis on the principal malaria vector Anopheles darlingi and on the occurrence of other possible vector species. Peridomestic human bait collections of adult mosquitoes and collections of larvae were made in many localities with a recent history of malaria transmission. Stable populations of An. darlingi were only found in the interior, south of the limit of tidal influence, due to year-round availability of breeding habitats in quiet partly sunlit places in flooded forest areas and along river banks. In the area with tidal movement of the rivers, breeding is limited to flooded areas in the wet season. Anopheles darlingi was only incidentally collected in low densities. In the interior, malaria transmission occurred in all places where An. darlingi was found. The absence of malaria transmission along the Upper Suriname River could be explained by the absence of An. darlingi. In the malaria endemic areas, An. darlingi was the most numerous mosquito biting on man. In the tidal region, malaria outbreaks are infrequent and might be explained by the temporary availability of favourable breeding habitats for An. darlingi. However, evidence is insufficient to incriminate An. darlingi as the vector of malaria in this region and the possible vectorial role of other anophelines is discussed.     

Observations on Ichthyosporidium giganteum (Microsporida) with particular reference to the host-parasite relations during merogony

Connective tissue cells, particularly fibroblasts, of the fish Leiostomus xanthurus Lacépède respond to the invading microsporidian parasite Ichthyosporidium sp. [assumed to be identical with Ichthyosporidium giganteum (Thélohan)] by proliferating themselves, coalescing into a syncytium, synthesizing copious amounts of cytoplasm around the parasites, and walling off the parasitized islands of cytoplasm with fibrous capsules. The resulting cysts are xenoparasitic complexes of the syncytial xenoma type, clearly different from the cell hypertrophy tumor (xenoma sensu Weissenberg) exemplified by the Glugea cyst. These findings involve a new concept of the structure and host-parasite relations of Ichthyosporidium. Formerly, the parasitized masses of cytoplasm were interpreted as extracellular plasmodial stages of the parasite (stages uncharacteristic of the microsporidia), while the parasites themselves were interpreted as nuclei of the "plasmodia." Actually, the parasite undergoes merogony in parasitophorous vacuoles which coalesce before sporogony begins. The nuclei of the mermonts are very small chromatin granules, becoming transformed into large basophilic diplokarya of the sporonts. Sporulation is diplokaryotic throughout, the diplokarya becoming reduced in size through 2 steps during sporogony.     

Observations on the ultrastructure of nucleated erythrocytes and thrombocytes,with particular reference to the structural basis of their discoidal shape

Summary The marginal band of nucleated erythrocytes in the toadfish is found, in electron micrographs, to be composed of about twenty-five microtubules approximately 200 Å in diameter. These form a bundle that encircles the erythrocyte just beneath the plasma membrane. These observations support the interpretation of Meves 1904, that this relatively stiff equatorial band may contribute to the maintenance of the discoid shape of nucleated erythrocytes in fish, amphibians, reptiles and birds.Similar microtubules form an annular bundle encircling the nucleus in fish thrombocytes. The number of tubular elements involved here is in excess of one hundred and they are located deep to the ectoplasmic layer instead of immediately beneath the plasmalemma. The term endoplasmic ring is therefore proposed for this structure.Comparative observations on nucleated erythrocytes of various species are presented showing that the density and fine structure of the material occupying the interchromosomal areas of the nucleus, always matches the cytoplasm and is related to the hemoglobin concentration of the species. These ultrastructural observations are consistent with the optical absorption and biochemical findings of other investigators indicating the presence of intranuclear hemoglobin in nucleated erythrocytes. Crystalline order is occasionally found in electron micrographs of the hemoglobin rich areas of the nucleus in toadfish erythrocytes but is not found in the cytoplasm.This research was supported by grant G-12916 of the National Science Foundation.     

Fine structure of developing hagfish erythrocytes with particular reference to the cytoplasmic organelles

Cytological changes accompanying the maturation of erythrocytes in the “Pacific hagfish” (Eptatretus stoutii) were studied. Great numbers of immature and mitotically dividing red blood cells in the peripheral circulation of the hagfish appear to indicate that extensive differentiation and proliferation occurs in the blood stream of this animal. The immature erythrocytes contained mitochondria, Golgi membranes, centrioles, microtubules and a high density of ribosomes in the cytoplasm. Intermediate stages revealed lysosomes in the cytoplasm. With progressive differentiation the hagfish erythrocytes accumulate hemoglobin and lose most of their cytoplasmic organelles. The various cytoplasmic organelles are apparently lost through a degradation process brought about by lysosomal autolysis. The undigested products of degradation such as mitochondrial and other intercellular membranes are apparently extruded by way of the plasma membrane. The plasma membrane of young as well as mature erythrocytes display evidence of intense pinocytotic activity. The nucleolus undergoes a reduction in size with progressive maturation. The cytoplasm of mature erythrocytes consists predominantly of hemoglobin. An equatorial microtubular marginal band is identifiable in differentiating erythrocytes.     

The forms of native chlorophyll in Chlamydobotrys stellata and their changes during adaptation from photo-heterotrophic to autotrophic growth

Summary Absorption and fluorescence spectra were measured for Chlamydobotrys stellata cultured either photo-heterotrophically on acetate or autotrophically on CO₂ as well as during adaptation from hetero- to autotrophic conditions. Curve analyses of the absorption spectra at liquid nitrogen temperature suggest the presence of chlorophyll-a forms with their main absorption peaks at 663, 670, 678, 685, 693 and 707 nm. The proportion of the longer wavelength forms, 685, 693 and 707 nm, decreases during adaptation to autotrophic growth. The chlorophyll-b content of the photo-heterotrophic culture was very low.Dedicated to Prof. Dr. A. Pirson on the occasion of his 60th birthday.     

Observations on the fine structure of a methane-oxidizing bacterium

Structural details of membranes, intracellular organelles, and the cell wall of a methane-utilizing bacterium identified as aMethylomonas sp. were studied by electron microscopy. The cell wall structure is similar to that found in other gram-negative bacteria. The cytoplasmic membrane shows invaginations, presumably forming the internal membrane bundles. Two types of polar organelles were encountered. In older cells myelin-like structures were observed. Under certain cultural conditions bleb-formation and possibly accumulation of reserve materials occurred. We wish to thank Mrs. M. H. Bakker-van der Velden, Mrs. W. H. Batenburgvan der Vegte and Miss J. C. de Bruyn for making the electron microscopical preparations and photographs. Thanks are due to Mr R. S. M. Revell of the Philips E. M. Application Laboratory, Eindhoven, for the photographs with the goniometer attachment.