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1.
Richard E. Triemer 《Journal of phycology》1982,18(3):399-411
Mitosis is described in the flagellate Oxyrrhis marina Dujardin and is compared in related genera. Dense plaques develop in the nuclear envelope at prophase and give rise to an intranuclear spindle. Some of the microtubules associate with the chromosomes while others extend across the nucleus. The basal bodies migrate toward the poles early in division and retain a position lateral to the nuclear poles throughout mitosis. Microtubules are not present between the nucleus and the basal bodies. The nucleolus is persistent and elongates throughout anaphase and telophase. Chromosomal separation is accomplished by sliding of non-chromosomal microtubules and by elongation of the nuclear envelope rather than by shortening of the spindle microtubules. The nuclear envelope begins to constrict in the center early in anaphase. Continued constriction of the envelope and elongation of the nucleus leads to the formation of a dumbbell-shaped nucleus by late telophase. Mitosis culminates by the constriction of the nucleus into two daughter nuclei. The taxonomic position of Oxyrrhis marina is discussed in light of these findings. 相似文献
2.
The formation of the nuclear envelope in the mitosis ofSpirogyra was studied with an electron microscope. The nuclear envelope was disrupted around the spindle equator in the metaphase.
Many small vesicles were observed in the metaphase spindle. These vesicles surrounded the masses of chromosomes and nucleolar
substance in the early anaphase, and they fused with each other to form daughter nuclear envelopes during the early anaphase.
The formation of new envelopes from small vesicles at such an early mitotic anaphase is reported here for the first time.
The possible origin of these vesicles is also discussed. 相似文献
3.
Summary The three-dimensional ultrastructural organization of the mitotic apparatus ofDimastigella mimosa was studied by computer-aided, serial-section reconstruction. The nuclear envelope remains intact during nuclear division. During mitosis, chromosomes do not condense, whereas intranuclear microtubules are found in close association with six pairs of kinetochores. No discrete microtubule-organizing centers, except kinetochore pairs, could be found within the nucleus. The intranuclear microtubules form six separate bundles oriented at different angles to each other. Each bundle contains up to 8 tightly packed microtubules which push the daughter kinetochores apart. At late anaphase only, midzones of these bundles align along an extended interzonal spindle within the narrow isthmus between segregating progeny nuclei. The nuclear division inD. mimosa can be described as closed intranuclear mitosis with acentric and separate microtubular bundles and weakly condensed chromosomes.Abbreviation MTOC
microtubule-organizing center 相似文献
4.
Taizo Motomura Chikako Nagasato Yoshibumi Komeda Kazuo Okuda 《Journal of phycology》2001,37(5):783-792
Mitosis in Boergesenia forbesii (Harvey) Feldman was studied by immunofluorescence microscopy using anti-β–tubulin, anti-γ–tubulin, and anti-centrin antibodies. In the interphase nucleus, one, two, or rarely three anti-centrin staining spots were located around the nucleus, indicating the existence of centrioles. Microtubules (MTs) elongated randomly from the circumference of the nuclear envelope, but distinct microtubule organizing centers could not be observed. In prophase, MTs located around the interphase nuclei became fragmented and eventually disappeared. Instead, numerous MTs elongated along the nuclear envelope from the discrete anti-centrin staining spots. Anti-centrin staining spots duplicated and migrated to the two mitotic poles. γ–Tubulin was not detected at the centrioles during interphase but began to localize there from prophase onward. The mitotic spindle in B. forbesii was a typical closed type, the nuclear envelope remaining intact during nuclear division. From late prophase, accompanying the chromosome condensation, spindle MTs could be observed within the nuclear envelope. A bipolar mitotic spindle was formed at metaphase, when the most intense staining of γ-tubulin around the centrioles could also be seen. Both spindle MT poles were formed inside the nuclear envelope, independent of the position of the centrioles outside. In early anaphase, MTs between separating daughter chromosomes were not detected. Afterward, characteristic interzonal spindle MTs developed and separated both sets of the daughter chromosomes. From late anaphase to telophase, γ-tubulin could not be detected around the centrioles and MT radiation from the centrioles became diminished at both poles. γ-Tubulin was not detected at the ends of the interzonal spindle fibers. When MTs were depolymerized with amiprophos methyl during mitosis, γ-tubulin localization around the centrioles was clearly confirmed. Moreover, an influx of tubulin molecules into the nucleus for the mitotic spindle occurred at chromosome condensation in mitosis. 相似文献
5.
Mitosis and cell division have been examined ultrastructurally in the vegetative cells of Hydrurus foetidus (Vill) Trev. and found to resemble that of Ochromonas in two important aspects. First, the rhizoplast acts as the spindle organizing body and second, the spindle elongates considerably during anaphase. It differs from Ochromonas in that there is no movement of the basal bodies and flagella towards the poles. Moreover, the nuclear envelope remains relatively intact throughout early stages of mitosis, with gaps developing at the poles during prophase to permit entry of spindle microtubules. Disruption of the nuclear envelope does not occur in the equatorial plane until late anaphase. The spindle persists into telophase and is bent towards the posterior of the cell by the ingrowing edge of the cleavage furrow. Persistence of the spindle and lack of Ochromoms-type cell elongation may be related to the constricting presence of the sheath during cell division—a completely different strategy to that adopted by the green algae under conditions of similar constraint. 相似文献
6.
Vegetative nuclear division in Ceratocystis fagacearum (Bretz) Hunt was found to differ from classical mitosis in that: (1) division always occurs perpendicular to the longitudinal axis of the cell, (2) anaphase movement is unilateral and unsynchronized, (3) a spindle occurs only between separating chromatids. Interphase and prophase nuclei and nucleoli are morphologically similar to those in higher plants. At metaphase the associated chromosomes form a bar of chromatin and lie against the hyphal wall. Spindle fibers appear between separating chromatids, perhaps pushing them apart. When nuclear division is complete the nuclei become attenuated and migrate. Vegetative nuclear division in C. fagacearum may be an evolutionary form of classical mitosis. 相似文献
7.
ELECTRON MICROSCOPIC STUDIES OF MITOSIS IN AMEBAE : II. The Giant Ameba Pelomyxa carolinensis
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Dividing nuclei from the giant ameba Pelomyxa carolinensis were fixed in osmium tetroxide solutions buffered with veronal acetate to pH 8.0. If divalent cations (0.002 M calcium, magnesium, or strontium as chlorides) were added to the fixation solution, fibrils that are 14 mµ in diameter and have a dense cortex are observed in the spindle. If the divalent ions were omitted, oriented particles of smaller size are present and fibrils are not obvious. The stages of mitosis were observed and spindle components compared. Fibrils fixed in the presence of calcium ions are not so well defined in early metaphase as later, but otherwise have the same diameter in the late metaphase, anaphase, and early telophase. Fibrils are surrounded by clouds of fine material except in early telophase, when they are formed into tight bundles lying in the cytoplasm unattached to nuclei. Metaphase and anaphase fibrils fixed without calcium ions are less well defined and are not observably different from each other. The observations are consistent with the concept that spindle fibrils are composed of polymerized, oriented protein molecules that are in equilibrium with and bathed in non-oriented molecules of the same protein. Partially formed spindle fibrils and ribosome-like particles were observed in the mixoplasm when the nuclear envelope had only small discontinuities. Remnants of the envelope are visible throughout division and are probably incorporated into the new envelope in the telophase. Ribosome-like particles are numerous in the metaphase and anaphase spindle but are not seen in the telophase nucleus, once the envelope is reestablished, or in the interphase nucleus. 相似文献
8.
K. Wells 《Protoplasma》1978,94(1-2):83-108
Summary The two division of meiosis that occur in the distal portion of the basidia ofPholiota terrestris were studied with light and electron microscopy. A diglobular spindle pole body (SPB), consisting of two globular elements and a connecting, electron-dense middle piece, is closely attached to the nuclear envelope of the fusion nucleus. During prometaphase I the globular elements separate and pass to the opposite poles as the chiastic spindle is formed. Evidently, the middle piece also separates with each resulting half persisting as an eccentric, electron-dense portion of the monoglobular SPB of meta-, ana-, and telophase nuclei. Also during prometaphase I, the nuclear envelope becomes discontinuous, especially in the lower region of the spindle. Light microscopic evidence of nucleolar extrusion at prometaphase I and II was observed. At metaphase I the SPB's move away from the condensed chromatic mass as the chromatids move asynchronously along the expanding spindle, evidently, due both to the elongation of the continuous fibers and the shortening of the chromosomal fibers. Two images resembling typical kinetochroes are illustrated in anaphase I nuclei, and others were seen during the study. At early telophase I and II the nuclear envelope is present laterally, is then formed in the interpolar region, and eventually appears between the chromatin and monoglobular SPB. A perforated ER cap, which is penetrated by microtubules, delimits the SPB. The nucleus enlarges, the chromatin becomes diffused except adjacent to the SPB, and the perinuclear ER becomes uniformly oriented around the nuclear envelope. At interphase I a diglobular SPB was not clearly documented. During interphase I the ER cap disappears but the perinuclear ER persists. Division II, with the exception of prophase, is essentially identical to division I. The postmeiotic, haploid nuclei migrate to the median or proximal region of the basidium. The diglobular SPB reappears. The meiotic apparatus inP. terrestris is considered to have the same fundamental features as those of plants and animals and in detail conforms to the pattern described in several light and electron microscopic studies of other Homobasidiomycetes. 相似文献
9.
Identification of the Meiotic Division of Malarial Parasites 总被引:3,自引:0,他引:3
Zygotes of Plasmodium berghei were cultured 15–25 h in vitro to yield mature infective ookinetes. Samples taken in the first 5 h of culture were examined by electron microscopy. Meiotic figures were detected in the nuclei of the zygotes. Threadlike leptotene chromatids (chromosomes) condensed from attachment plaques on the nuclear envelope; chromatid pairing followed (zygotene), with synaptonemal complexes subsequently appearing (pachytene). These complexes persisted into metaphase but dissociated when the chromatids rapidly decondensed during anaphase. At telophase of the first meiotic division the kinetochores were retracted toward two small spindle complexes, which were found at widely separated poles in the nuclear envelope. The observations are consistent with a haploid genome of 8–10 chromosomes. 相似文献
10.
At the ultrastructural level, cell division in Ochromonas danica exhibits several unusual features. During interphase, the basal bodies of the 2 flagella replicate and the chloroplast divides by constriction between its 2 lobes. The rhizoplast, which is a fibrous striated root attached to the basal body of the long flagellum, extends under the Golgi body to the surface of the nucleus in interphase cells. During proprophase, the Golgi body replicates, apparently by division, and a daughter rhizoplast, appears. During prophase, the 2 pairs of flagellar basal bodies, each with their accompanying rhizoplast and Golgi body, begin to separate. Three or 4 flagella are already present at this stage. At the same time, there is a proliferation of microtubules outside the nuclear envelope. Gaps then appear in the nuclear envelope, admitting the microtubules into the nucleus, where they form a spindle. A unique feature of mitosis in O. danica is that the 2 rhizoplasts form the poles of the spindle, spindle microtubules inserting directly onto the rhizoplasts. Some of the spindle microtubules extend from pole to pole; others appear to attach to the chromosomes. Kinetochores, however, are not present. The nuclear envelope breaks down, except, in the regions adjacent, to the chloroplasts; chloroplast ER remains intact throughout mitosis. At late anaphase the chromosomes come to lie against part of the chloroplast ER. This segment of the chloroplast ER appears to be incorporated as part of the reforming nuclear envelope, thus reestablishing the characteristic nuclear envelope—chloroplast ER association of the interphase cell. 相似文献
11.
The distribution of ubiquitin protein in meristematic mesophyll cells of barley (Hordeum vulgare L.) leaves was investigated by using immunofluorescence microscopy. Simultaneous observation of nuclei was achieved byDAPI (4 6-diamidino-2-phenylindol-dihydrochloride) staining. A strong correlation between the chromatin organisation and the ubiquitin
distribution could be observed. Interphase nuclei revealed an intense content of ubiquitin and accumulation of ubiquitin at
the nuclear envelope, whereas condensed chromosomes of dividing cells excluded any ubiquitin appearance. During cell division,
the aggregation of ubiquitin protein was detected in the area of the mitotic spindle in anaphase as well as the area of the
cell plate in the late telophase. 相似文献
12.
Urs-Peter Roos 《Chromosoma》1973,40(1):43-82
Rat kangaroo (PtK2) cells were fixed and embedded in situ. Cells in mitosis were studied with the light microscope and thin sections examined with the electron microscope. Pericentriolar, osmiophilic material, rather than the centrioles, is probably involved in the formation of astral microtubules during prophase. Centriole migration occurs during prophase and early prometaphase. The nuclear envelope ruptures first in the vicinity of the asters. Nuclear pore complexes disintegrate as envelope fragments are dispersed to the periphery of the mitotic spindle. Microtubules invade the nucleus through gaps of the fragmented envelope. The number of microtubules and the degree of spindle organization increase during prometaphase and are maximal at metaphase. At this stage, chromosomes are aligned on the spindle equator, sister kinetochores facing opposite poles. Cytoplasmic organelles are excluded from the spindle. Prominent bundles of kinetochore microtubules converge towards the poles. Spindles in cold-treated cells consist almost exclusively of kinetochore tubules. Separating daughter chromosomes in early anaphase are connected by chromatin strands, possibly reflecting the rupturing of fibrous connections occasionally observed between sister chromatids in prometaphase. Breakdown of the spindle progresses from late anaphase to telophase, except for the stem bodies. Chromosomes decondense to form two masses. Nuclear envelope reconstruction, probably involving endoplasmic reticulum, begins on the lateral faces. Nuclear pores reappear on membrane segments in contact with chromatin. Microtubules are absent from reconstructed daughter nuclei.This report is to a large part based on a dissertation submitted by the author to the Graduate Council of the University of Florida in partial fulfillment of the requirements for the degree of Doctor of Philosophy. 相似文献
13.
Dividing cells of Spirogyra sp. were examined with both the light and electron microscopes. By preprophase many of the typical transverse wall micro-tubules disappeared while others were seen in the thickened cytoplasmic strands. Microtubules appeared in the polar cytoplasm at prophase and by prometaphase they penetrated the nucleus. They were attached to chromosomes at metaphase and early anaphase, and formed a sheath surrounding the spindle during anaphase; they were seen in the interzonal strands and cytoplasmic strands at telophase. The interphase nucleolus, containing 2 distinct zones and chromatinlike material, fragmented at prophase; at metaphase and anaphase nucleolar material coated the chromosomes, obscuring them by late anaphase. The chromosomes condensed in the nucleoplasm at prophase, moving into the nucleolus at prometaphase. The nuclear envelope was finally disrupted at anaphase during spindle elongation; at telophase membrane profiles coated the reforming nuclei. During anaphase and early telophase the interzonal region contained vacuoles, a few micro-tubules, and sometimes eliminated n ucleolar material; most small organelles, including swollen endoplasmic reticulum and tubular membranes, were concentrated in the polar cytoplasm. Quantitative and qualitative cytological observations strongly suggest movement of intact wall rnicrotubules to the spindle at preprophase and then back again at telophase. 相似文献
14.
D. B. Gromov 《Protoplasma》1985,126(1-2):130-139
Summary The fine structure ofAmoeba proteus nuclei has been studied during interphase and mitosis. The interphase nucleus is discoidal, the nuclear envelope is provided with a honeycomb layer on the inside. There are numerous nucleoli at the periphery and many chromatin filaments and nuclear helices in the central part of nucleus.In prophase the nucleus becomes spherical, the numerous chromosomes are condensed, and the number of nucleoli decreases. The mitotic apparatus forms inside the nucleus in form of an acentric spindle. In metaphase the nuclear envelope loses its pore complexes and transforms into a system of rough endoplasmic reticulum cisternae (ERC) which separates the mitotic apparatus from the surrounding cytoplasm; the nucleoli and the honeycomb layer disappear completely. In anaphase the half-spindles become conical, and the system of ERC around the mitotic spindle persists. Electron dense material (possibly microtubule organizing centers—MTOCs) appears at the spindle pole regions during this stage. The spindle includes kinetochore microtubules attached to the chromosomes, and non-kinetochore ones which pierce the anaphase plate. In telophase the spindle disappears, the chromosomes decondense, and the nuclear envelope becomes reconstructed from the ERC. At this stage, nucleoli can already be revealed with the light microscope by silver staining; they are visible in ultrathin sections as numerous electron dense bodies at the periphery of the nucleus.The mitotic chromosomes consist of 10 nm fibers and have threelayered kinetochores. Single nuclear helices still occur at early stages of mitosis in the spindle region. 相似文献
15.
Chromosome elimination in Heteropeza pygmaea 总被引:1,自引:0,他引:1
René Camenzind 《Chromosoma》1974,49(1):87-98
Chromosome elimination in the 3rd cleavage division of the gall midge Heteropeza pygmaea was observed with the Differential Interference Contrast method and recorded with photomicrography and time-lapse cinémicrography. The chromosomes which move all the way to the poles (S-chromosomes) are included in the presumptive somatic nuclei while the lagging chromosomes are eliminated (E-chromosomes). In early prometaphase of an elimination division the nuclear envelope is replaced by the spindle envelope which persists until late telophase and separates nucleoplasm and cytoplasm. In prometaphase the volume of the spindle decreases considerably. Until mid-anaphase the E and the S-chromosomes cannot be distinguished from each other either morphologically or topologically and they both behave like chromosomes in a normal cleavage division. In early anaphase the velocity of the E-chromosomes is usually less than that of the S-chromosomes. After variable amounts of anaphase movement the E-chromosomes return towards the equator with a velocity which is less than their velocity in early anaphase. Their kinetochores are still oriented towards the poles. The two chromatids of an E-chromosome usually move symmetrically towards the poles and back to the equator. At the time when the E-chromosomes stop moving towards the poles the S-chromosomes sometimes accelerate. 相似文献
16.
Synchronously dividing binuclear cells were induced in root tips ofTriticum turgidum by caffeine treatment. Spindle and other microtubular configurations of such cells were studied using tubulin immunofluorescence
and electron microscopy. The binuclear cells developed one, two or three preprophase microtubule bands longitudinally, transversely
or rarely in a cross configuration. During the mitotic entry binuclear cells formed prophase spindles separately around each
nucleus. When the nuclei were located fairly apart, their spindle structures developed independently throughout all mitotic
phases. But when the nuclei were located closely together their metaphase and anaphase spindles shared a common polar region.
However, the two spindles in such cells retained their functional autonomy. They display structurally independent minipoles
in the common polar region. After anaphase the neighbouring nonsister chromosome groups of nuclei divided by a common polar
region come to lie close together and in telophase, become enclosed by a common nuclear envelope. During cytokinesis of binuclear
cells cell plates were formed only between sister nuclei. These cell plates may develop normally or may curve or branch giving
rise to aberrant daughter cell walls. The peculiar mode of spindle and spindle polar region organization of binuclear cells
and determination of the division plane in them are discussed. 相似文献
17.
Martha J. Powell 《American journal of botany》1980,67(6):839-853
The structure of centric, intranuclear mitosis and of organelles associated with nuclei are described in developing zoosporangia of the chytrid Rhizophydium spherotheca. Frequently dictyosomes partially encompass the sides of diplosomes (paired centrioles). A single, incomplete layer of endoplasmic reticulum with tubular connections to the nuclear envelope is found around dividing nuclei. The nuclear envelope remains intact during mitosis except for polar fenestrae which appear during spindle incursion. During prophase, when diplosomes first define the nuclear poles, secondary centrioles occur adjacent and at right angles to the sides of primary centrioles. By late metaphase the centrioles in a diplosome are positioned at a 40° angle to each other and are joined by an electron-dense band; by telophase the centrioles lie almost parallel to each other. Astral microtubules radiate into the cytoplasm from centrioles during interphase, but by metaphase few cytoplasmic microtubules are found. Cytoplasmic microtubules increase during late anaphase and telophase as spindle microtubules gradually disappear. The mitotic spindle, which contains chromosomal and interzonal microtubules, converges at the base of the primary centriole. Throughout mitosis the semipersistent nucleolus is adjacent to the nuclear envelope and remains in the interzonal region of the nucleus as chromosomes separate and the nucleus elongates. During telophase the nuclear envelope constricts around the chromosomal mass, and the daughter nuclei separate from each end of the interzonal region of the nucleus. The envelope of the interzonal region is relatively intact and encircles the nucleolus, but later the membranes of the interzonal region scatter and the nucleolus disperses. The structure of the mitotic apparatus is similar to that of the chytrid Phlyctochytrium irregulare. 相似文献
18.
Fine structure of division in ciliate protozoa. I. Micronuclear mitosis in Blepharisma 总被引:8,自引:7,他引:1
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R A Jenkins 《The Journal of cell biology》1967,34(2):463-481
The mitotic, micronuclear division of the heterotrichous genus Blepharisma has been studied by electron microscopy. Dividing ciliates were selected from clone-derived mass cultures and fixed for electron microscopy by exposure to the vapor of 2% osmium tetroxide; individual Blepharisma were encapsulated and sectioned. Distinctive features of the mitosis are the presence of an intact nuclear envelope during the entire process and the absence of centrioles at the polar ends of the micronuclear figures. Spindle microtubules (SMT) first appear in advance of chromosome alignment, become more numerous and precisely aligned by metaphase, lengthen greatly in anaphase, and persist through telophase. Distinct chromosomal and continuous SMT are present. At telophase, daughter nuclei are separated by a spindle elongation of more than 40 µ, and a new nuclear envelope is formed in close apposition to the chromatin mass of each daughter nucleus and excludes the great amount of spindle material formed during division. The original nuclear envelope which has remained structurally intact then becomes discontinuous and releases the newly formed nucleus into the cytoplasm. The micronuclear envelope seems to lack the conspicuous pores that are typical of nuclear envelopes. The morphology, size, formation, and function of SMT and the nature of micronuclear division are discussed. 相似文献
19.
The cell division cycle of Hymenomonas carterae (Braarud et Fagerland) Braarud was investigated at the ultrastructural level. DNA synthesis and cytokinesis occurred during the 8-hour dark period. All organelles, including the flagellar bases were replicated prior to nuclear division. Prophase consisted of a clustering of the chromosomes into distinct groups and the disappearance of the nucleolus. During metaphase there was complete dissociation of the nuclear envelope resulting in the formation of an open spindle containing no major organelles. The metaphase plate formed at right angles to an imaginary line joining the two pairs of flagellar bases. Elongation of the cell and separation of the chromosomes occurred at anaphase. During early telophase the nuclear envelope veformed and was closely associated with the chromosome masses, resulting in the nuclear possessing convoluted profiles. Telophase was characterized by complete break down of spindle fibres, rounding off of the nuclear profiles, reappearance of the nucleolus, emergence of the flagella and the final separation of the two daughter cells. 相似文献
20.
Summary Mitosis in the dermatophyteMicrosporum canis was studied by freeze substitution and electron microscopy, and analyzed by three dimensional reconstruction from serial sections of the mitotic nuclei. The interphase nucleus has associated nucleus-associated organelle (NAO) on a portion of the outer surface of the nuclear envelope, subjacent to which there was dense intranuclear material. The NAO divided and separated on the envelope, and a spindle was formed. The spindle was composed mostly of microtubules extended between opposite NAOs. Pairing of kinetochores was observed in the spindle from an early stage of development, when chromosomes were not so condensed, and remained unchanged while chromosome condensation proceeded until metaphase. Before the completion of nuclear division, daughter nuclei were connected by a narrow spindle channel, and then the nucleolus, whose structure underwent minimal change during mitosis, was eliminated into the cytoplasm. 相似文献