共查询到18条相似文献,搜索用时 171 毫秒
1.
2.
血栓溶解酶产生菌及其培养条件的研究 总被引:12,自引:1,他引:11
通过对根霉12号发酵液的分析及发酵条件的研究,发现其发酵液中含有能溶解血栓的物质,但不能分解血细胞。它在麸皮胰蛋白胨培养基(pH5.1)上30℃振荡培养48~60h,pH值达到7.4左右时产生血栓溶解酶的活力最高。 相似文献
3.
根霉12号固体发酵产生纤溶酶工艺条件的研究 总被引:15,自引:0,他引:15
研究了根霉12号固体发酵产生纤溶酶的工艺条件。采用单因素试验、均匀设计方法对固体发酵培养基的碳源、氮源、碳氮比、初始pH、加水量、无机盐加量进行了优化;采用正交试验对发酵时间、接种量进行了研究。结果表明,实验范围内根霉12固体发酵产纤溶酶的适宜培养基组成为:麸皮:豆粕=1:2,初始pH5.0,加水量0.75ml/g物料,MnSO4-H2O 和 (NH4)2SO4加量分别为0.25%和1.42%(对物料)。适宜培养条件为接种量10^7个孢子/g 物料,培养时间 72h。优化条件下的纤溶酶产量平均达 791.81u/g物料。 相似文献
4.
一株具有纤溶活性的枯草杆菌(Bacillus Subtilis)的研究——液体发酵条件的选择 总被引:5,自引:0,他引:5
本文主要报告了,以BacilusSubtilisHW-12为试验菌株,在液体发酵中,所确定的发酵条件为:碳源是木糖,氮源是大豆胰蛋白胨,发酵液pH7.0,培养温度37℃,培养时间96小时。在这样的发酵条件下,该菌产酶活力为200尿激酶单位/毫升发酵液 。 相似文献
5.
6.
研究了嗜碱芽孢杆菌(alkalophilicBaclussp.)NTT33发酵产生胞外碱性β-甘露聚糖酶的条件,其最佳碳源为1%槐豆角,最佳氮源为1%蛋白胨+0.2%酵母膏,发酵培养36h产酶量最高(达61.3υ/mL)。甘油,葡萄糖,甘露糖等对产酶有强的阻遏作用。该菌株经紫外诱变处理后,采用透明圈法初步筛选出在含葡萄糖和不含葡萄糖的槐豆胶培养基上同时产生透明圈的菌株,进一步测定其产酶进程曲线,最后筛选到一株(NTT33-r6)部分消除葡萄糖代谢阻遏高产β-甘露聚糖酶的菌株,其酶活力比出发菌株提高50%,,达到96.3U/ml;。 相似文献
7.
根霉cw-1产纤溶酶的发酵条件优化 总被引:1,自引:0,他引:1
《生物技术世界》2016,(5)
目的:确定根霉cw-1高产纤溶酶的液体发酵培养基组成及培养条件,使纤溶酶产量得到明显提高。方法和结果:采用响应面法对该菌种产纤溶酶的发酵条件进行优化。首先利用Plackett Burman试验设计筛选出影响产酶量的3个主要因素,即麸皮的浓度、豆粕的浓度和初始p H。在此基础上使用Design-Expert软件进行Box-Behnken试验设计,通过响应面分析得出菌株cw-1发酵产酶的最佳条件为:麸皮28.67g/L、豆粕46.28g/L、初始p H 5.8。经过试验验证,优化后粗酶液酶活达到243.22 U/m L(尿激酶单位),与响应面预测结果一致,较优化前酶活提高41倍。 相似文献
8.
9.
目的:目前临床使用的溶栓药物疗效肯定,但还存在许多缺陷,而且价格昂贵,因此研制新型溶栓药物的需求迫切。方法:研究了根霉Rhizopus chinensisYY-15液体摇瓶发酵产生纤溶酶的工艺条件。采用单因素试验对液体发酵培养基的碳源、氮源、碳氮比、初始pH进行了优化;采用正交试验对发酵时间、接种量进行了研究。结果:实验范围内菌株液体发酵产纤溶酶的适宜培养基组成为:麸皮水浓度3%(w/v),豆粕浓度5%(w/v),初始培养基pH5.0。适宜培养条件为接种量6%,培养时间72h。优化条件下的摇瓶液体发酵纤溶酶产量平均达98.31 U/ml。 相似文献
10.
一株根霉产脂肪酶发酵条件的研究 总被引:13,自引:0,他引:13
通过对筛选出的一株根梅RhizopusY-92产旨肪肪酶发酵2基组成(C,N,无机盐)及工艺条件的探索,并经正交实验,得到较优培养基配方为:蛋白胨6%,豆饼粉4%,葡萄糖1%,MgSO4·7H2O0.1%,KH2PO40.3%,起始PH8.0,在此条件下,发酵液酶活可达99.15u/ml,是初始酶活的178%。 相似文献
11.
【目的】通过响应面试验对产纤溶酶菌株CNY16发酵条件进行优化,并对其酶学特性进行初步研究。【方法】采用Plackett-Burman设计得出酵母膏、氯化钠、转速3个最重要影响因素,通过最陡爬坡实验逼近酶活的最高区域,然后根据Box-Behnken中心组合设计实验对显著因素进行优化分析,最后对该酶学性质进行初步分析。【结果】最终得到3个因素的最优组合:酵母膏3.28%,氯化钠1.14%,转速166 r/min,在此培养条件下,纤溶酶活达到875.932 U/mL,比优化前提高了46%;该菌株产纤溶酶最适温度为30°C,最适pH为6.5。【结论】确定了高产纤溶酶菌株CNY16的最优发酵条件及其部分酶学性质,为该酶的进一步深入研究及中试实验奠定基础。 相似文献
12.
【目的】以发酵液纤溶酶活力为指标,优化海洋来源的链霉菌菌株MY0504的发酵条件。【方法】在菌株生长曲线及单因素试验基础上,采用Plackett-Burman设计筛选影响纤溶酶活性的主要因素,进一步用最陡爬坡试验及Box-Behnken中心组合设计法优化发酵条件。【结果】纤溶酶活性最高的发酵条件为:葡萄糖21.68 g/L,酵母粉25.31 g/L,NaCl5.0 g/L,K_2HPO_4·3H_2O3.0 g/L,MgSO_4·7H_2O 0.5 g/L,FeSO_4·7H_2O 0.02 g/L,装液量50 mL(250 mL摇瓶),接种量10%(体积比),初始pH 7.5,温度24°C,转速200 r/min,培养时间4.5 d。发酵液纤溶酶活性可达2 190.6 U/mL。【结论】确定了MY0504菌株产纤溶酶的最优发酵条件,为该酶的进一步分离纯化及性质研究奠定基础。 相似文献
13.
The central composite rotable design (CCRD) was used to determine optimal conditions for fibrinolytic enzyme production by Bacillus subtilis DC-2 in poly-ethylene glycol 4000 (PEG 4000) and sodium sulfate (Na(2)SO(4)) aqueous two-phase system (ATPS). PEG 4000 and Na(2)SO(4) concentration, fermentation time and temperature, and pH were selected as variables to evaluate the fibrinolytic activity in PEG phase. Using response surface methodology (RSM), a second-order polynomial equation was obtained by multiple regression analysis. The predicted maximal fibrinolytic activity in PEG phase was 1241.02 IU/ml with 9.05% PEG 4000 concentration, 5.06% Na(2)SO(4) concentration, 118.77 h fermentation time, 37.57 degrees C fermentation temperature and pH 6.52. The validity of the response model was verified by a good agreement between predicted and experimental results. The fibrinolytic activity obtained from experimental results in PEG phase (1223.61 IU/ml) was higher than that produced in homogeneous fermentation (1165.58 IU/ml). 相似文献
14.
Solid state fermentation of rice chaff for fibrinolytic enzyme production by Fusarium oxysporum 总被引:1,自引:0,他引:1
Rice chaff was used as the substrate for the production of fibrinolytic enzyme by Fusarium oxysporum in solid state fermentation.
The optimized moisture content of the medium was 30 - 50% (v/w). An inoculum of 5 - 10% (v/v) and an average particle size
of 400 μm for the substrate were optimum for productivity and enzyme activity of 80 IU per ml filtrate was achieved after
96 hours of fermentation.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
15.
Hydrogel matrices based on starch and dimethylaminoethyl methacrylate (Starch/DMAEMA) were synthesized including γ-irradiation as a clean initiator. The prepared hydrogels were characterized in terms of their gel fraction yield, degree of equilibrium swelling. The prepared hydrogels were examined as carriers for immobilization of Bacillus subtilis that has the ability to secrete an extracellular fibrinolytic enzyme that degrades fibrin. Scanning electron microscope (SEM) analysis showed proliferation of the bacterial cells entrapped inside the polymeric matrix. The immobilization process increases the production time of fibrinolytic enzyme up to 120 h instead of 96 h for the free cells. The optimum temperature of activity broadened and a significant shift in the pH optima was observed upon immobilization. The reusability of immobilized cells under repeated batch fermentation conditions was also investigated. At the optimum production conditions, immobilization of B. subtilis cells onto Starch/DMAEMA resulted in a four fold increase in enzyme activity. 相似文献
16.
17.
18.
研究了根霉12号固体发酵产生纤溶酶的工艺条件。采用单因素试验、均匀设计方法对固体发酵培养基的碳源、氮源、碳氮比、初始pH、加水量、无机盐加量进行了优化;采用正交试验对发酵时间、接种量进行了研究。结果表明,实验范围内根霉12固体发酵产纤溶酶的适宜培养基组成为:麸皮∶豆粕=1∶2,初始pH5.0,加水量0.75ml/g物料, MnSO4H2O和 (NH4)2SO4加量分别为0.25%和 1.42%(对物料)。适宜培养条件为接种量107个孢子/g物料,培养时间72h。优化条件下的纤溶酶产量平均达791.81u/g物料。 相似文献