共查询到20条相似文献,搜索用时 31 毫秒
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Bidirectional promoters are defined as those that regulate adjacent genes organized in a divergent fashion (head to head orientation) and separated by < 1 kb. In order to dissect bidirectional promoter activity in a model plant, deletion analysis was performed for seven rice promoters using promoter-reporter gene constructs, which identified three promoters to be bidirectional. Regulatory elements located in or close to the 5′-untranslated regions (UTR) of one of the genes (divergent gene pair) were found to be responsible for their bidirectional activity. DNA footprinting analysis identified unique protein binding sites in these promoters. Deletion/alteration of these motifs resulted in significant loss of expression of the reporter genes on either side of the promoter. Changes in the motifs at both the positions resulted in a remarkable decrease in bidirectional activity of the reporter genes flanking the promoter. Based on our results, we propose a novel mechanism for the bidirectionality of rice bidirectional promoters. 相似文献
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Structure and regulation of the maize Bronze2 promoter 总被引:1,自引:0,他引:1
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Sascha Beneke Helga Bestgen Albrecht Klein 《Molecular genetics and genomics : MGG》1995,248(2):225-228
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Comparative genome analysis of potential regulatory elements in the ABCG5-ABCG8 gene cluster 总被引:3,自引:0,他引:3
Remaley AT Bark S Walts AD Freeman L Shulenin S Annilo T Elgin E Rhodes HE Joyce C Dean M Santamarina-Fojo S Brewer HB 《Biochemical and biophysical research communications》2002,295(2):276-282
The excretion of sterols from the liver and intestine is regulated by the ABCG5 and ABCG8 transporters. To identify potential regulatory elements, 152 kb of the human ABCG5-ABCG8 gene cluster was sequenced and comparative genome analysis was performed. The two genes are oriented in a head-to-head configuration and are separated by a 374-bp intergenic region, which is highly conserved among several species. Using a reporter construct, the intergenic region was found to act as a bidirectional promoter. A conserved GATA site in the intergenic region was shown by site-directed mutagenesis to act as a repressor for the ABCG5 promoter. The intergenic region was also shown to be partially responsive to treatment by LXR agonists. In summary, several potential regulatory elements were found for the ABCG5 and ABCG8 genes, and the intergenic region was found to act as a bidirectional promoter. 相似文献
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