共查询到20条相似文献,搜索用时 15 毫秒
1.
The effect of different temperatures on bud formation in excisedleaf fragments and in stem segments of Heloniopsis orientalis,a monocotyledonous plant, was investigated in light and in darkness.The optimal temperature for bud formation was 21?C to 25?C.16?C pretreatment for 7 to 21 days promoted bud formation inleaf segments. 30?C pretreatment for 7 days or more reducedthe number of buds in both young etiolated and mature greenleaf segments but not in young green leaf segments. In younggreen leaf segments grown in darkness, however, 30?C pretreatmentreduced the number of buds. Inhibition of bud formation dueto high temperature could not be reversed by BA. (Received November 15, 1978; ) 相似文献
2.
The position of adventitious bud formation on isolated leavesfrom young, green and etiolated plants of Heloniopsis orientaliswas considerably influenced by their orientation on the medium.Homogeneous distribution of buds over the whole surface of theleaves was observed in darkness, however, irrespective of theirorientation. The polarity of the bud regeneration was influencedby the application of various growth regulators, such as fusicoccin,cytokinins or abscisic acid. All attempts to induce the formationof adventitious buds on the abaxial side of the leaves wereunsuccessful. From the present and previous results, three polarityaxes (longitudinal, transverse and dorsiventral axes) in thesiting of bud formation on leaves are described. The gradientin the quantity and relative position of vascular bundles andchanges in the stomatal density of a leaf are discussed in termsof their possible roles in the determination of the sites ofbudding on isolated leaves. (Received March 9, 1981; Accepted August 28, 1981) 相似文献
3.
In plants held under long days in the vegetative stage, youngexpanding leaves of poinsettia (Euphorbia pulcherrima Willd.Brilliant Diamond) are the main source of axillarybud inhibition, while the apical bud, which includes the meristem,primordial leaves and small unfolded leaves, is a secondaryinhibition source. Removal of these expanding leaves resultedin rapid release and growth of axillary buds. Decapitation ofthe apical bud resulted in delayed axillary bud release. Inreproductive plants kept in short days, the pigmented bractsare the primary source of axillary bud inhibition and the cyathiaare the secondary source. Applications of NAA substitutedfor both young leaves and bract inhibition maintainedapical dominance. The concentration of endogenous auxin washighest in the apical bud. However, when calculated on wholeorgan basis the auxin level was greater in young developingvegetative leaves and in reproductive bracts than in the apicalbud. Euphorbia pulcherrima Willd, apical bud, apical dominance, auxin, correlative inhibition, cyathia, poinsettia, IAA, NAA 相似文献
4.
Optimum conditions were determined for in vivo growth and multiplicationof Garcinia mangostana L. using explants from aseptically germinatedseedlings and field-grown plants. Proliferating shoots wereobtained from cotyledon segments cultured on modified Murashigeand Skoog's (1962) medium with 6-benzylaminopurine. Juvenileleaf segments produced adventitious buds on Woody Plant Medium(Lloyd and McCown, 1981). Root segments gave few buds. Shoottip, nodal, and internodal explants gave multiple axillary andadventitious buds. Shoots were multiplied by enhanced axillaryand adventitious bud formation. The shoots were rooted withindolebutyric acid treatment. Rooted shoots were readily establishedin vermiculite: sand (1:1) mixture. Garcinia mangostana L., Mangosteen, tissue culture, shoot regeneration, bud development 相似文献
5.
Plantlet regeneration via organogenesis was achieved in callus cultures derived form mature leaves, stems and leaves, petioles and roots of young seedling of Psoralea corylifolia on Murashige and Skoog medium supplemented with 2.5–3.0 mg L-1 BA, 1.0 mg L-1 NAA and 3% (w/v) sucrose. The rate of shoot bud regeneration was positively correlated with the concentration of hormones in the nutrient media. Shoot buds regenerated more readily from juvenile explants (seedling source) as compared to the mature explants. Addition of adenine sulphate (5 mg L-1) to the culture medium increased the growth of shoot buds. Optimum responses were obtained in hypocotyl and leaf explants using NAA in combination with BA, the highest rate of shoot bud regeneration being in hypocotyl explants. Rooting was readily achieved on the differentiated shoots on MS basal media without growth regulators. Regenerated plantlets were successfully established in the greenhouse. 相似文献
6.
The outgrowth of lateral buds is known to be controlled by theupper shoot tissues, which include the apex, the young leavesand the upper stem. An analysis of the influence of these plantparts on axillary bud elongation in Ipomoea nil was carriedout by various treatments on these specific tissues. A restriction of elongation in the main shoot due to eitherdecapitation or shoot inversion resulted in the release of apicaldominance A non-linear type of compensating growth relationshipwas observed between the 13 cm apical growing region of thestem and the lateral buds. It was determined by decapitation,defoliation and AgNO3 treatments that both the 13 cm stem-growthregion and the young leaves (15 cm in length) had a muchgreater inhibitory influence on the outgrowth of specified lateralbuds than did the stem apex (consisting of the terminal 0.5cm of the shoot). The specified lateral buds which were analyzedfor outgrowth were located a number of nodes below the shootapex. The intervening nodes were debudded. Although the importanceof young leaves in the control of apical dominance has beenpreviously recognized, the most significant result from thepresent study with Ipomoea was the strong influence of the 13cm apical growth region of the stem on the out growth of thelateral buds. Apical dominance, Ipomoea nil L., Pharbitis nil, growth region, lateral bud outgrowth, decapitation, defoliation, shoot inversion 相似文献
7.
J. Šebánek 《Biologia Plantarum》1976,18(4):307-310
The role of exogenously supplied gibberellin (GA3) and cytokinin (benzyladenin -BA) in the correlation between the mature leaf and its axillary bud was investigated in one-node segments ofHydrangea. When both leaves were left on the segments, then both GA and BA were able to determine the dominance between axillary buds, that means that the bud treated with the corresponding growth regulator grew more vigorously. When one of the leaves was removed, the bud belonging to the removed leaf grew more vigorously, but GA applied onto the axillary bud belonging to the remaining leaf caused a complete correlation reversal: the bud belonging to the remaining leaf grew more vigorously. On the contrary, the application of BA onto the bud of the remaining leaf resulted in only insignificantly stimulated growth of the bud belonging to this leaf. 相似文献
8.
Effect of Assimilate Supply on Development and Growth Potential of Axillary Buds in Roses 总被引:1,自引:0,他引:1
The effect of assimilate supply on axillary bud developmentand subsequent shoot growth was investigated in roses. Differencesin assimilate supply were imposed by differential defoliation.Fresh and dry mass of axillary buds increased with increasedassimilate supply. The growth potential of buds was studiedeither by pruning the parent shoot above the bud, by graftingthe bud or by culturing the bud in vitro. Time until bud breakwas not clearly affected by assimilate supply during bud development,Increase in assimilate supply slightly increased the numberof leaves and leaf primordia in the bud; the number of leavespreceding the flower on the shoot grown from the axillary budsubstantially increased. No difference was found in the numberof leaves preceding the flower on shoots grown from buds attachedto the parent shoot and those from buds grafted on a cutting,indicating that at the moment of release from inhibition thebud meristem became determined to produce a specific numberof leaves and to develop into a flower. Assimilate supply duringaxillary bud development increased the number of pith cells,but the final size of the pith in the subsequent shoot was largelydetermined by cell enlargement, which was dependent on assimilatesupply during shoot growth. Shoot growth after release frominhibition was affected by assimilate supply during axillarybud development only when buds sprouted attached to the parentshoot, indicating that shoot growth is, to a major extent, dependenton the assimilate supply available while growth is taking place.Copyright1994, 1999 Academic Press Assimilate supply, axillary bud, cell number, cell size, defoliation, development, growth potential, meristem programming, pith, Rosa hybrida, rose, shoot growth 相似文献
9.
The effect of axillary bud age on the development and potentialfor growth of the bud into a shoot was studied in roses. Ageof the buds occupying a similar position on the plant variedfrom 'subtending leaf just unfolded' up to 1 year later. Withincreasing age of the axillary bud its dry mass, dry-matterpercentage and number of leaves, including leaf primordia, increased.The apical meristem of the axillary bud remained vegetativeas long as subjected to apical dominance, even for 1 year. The potential for growth of buds was studied either by pruningthe parent shoot above the bud, by grafting the bud or by culturingthe bud in vitro. When the correlative inhibition (i.e. dominationof the apical region over the axillary buds) was released, additionalleaves and eventually a flower formed. The number of additionalleaves decreased with increasing bud age and became more orless constant for axillary buds of shoots beyond the harvestablestage, while the total number of leaves preceding the flowerincreased. An increase in bud age was reflected in a greaternumber of scales, including transitional leaves, and in a greaternumber of non-elongated internodes of the subsequent shoot.Time until bud break slightly decreased with increasing budage; it was long, relatively, for 1 year old buds, when theysprouted attached to the parent shoot. Shoot length, mass andleaf area were not clearly affected by the age of the bud thatdeveloped into the shoot. With increasing bud age the numberof pith cells in the subsequent shoot increased, indicatinga greater potential diameter of the shoot. However, final diameterwas dependent on the assimilate supply after bud break. Axillarybuds obviously need a certain developmental stage to be ableto break. When released from correlative inhibition at an earlierstage, increased leaf initiation occurs before bud break.Copyright1994, 1999 Academic Press Age, axillary bud, cell number, cell size, pith, shoot growth, Rosa hybrida, rose 相似文献
10.
Bud Structure and Shoot Architecture of Canopy and Understorey Evergreen Broad-leaved Trees at their Northern Limit in East Asia 总被引:3,自引:3,他引:3
The morphology of winter buds, shoot growth and branching architecturewas studied in evergreen broad-leaved trees of subtropical/warm-temperaterain forests of southern and central Japan. Winter buds werecategorized into three types based on external morphology anddevelopmental processes: naked, hypsophyllary and scaled buds.Each shoot tip with intermittent growth was covered with a smallnumber of immature leaves or hypsophylls when growth ceased.Hypsophylls protect the apical meristem during its resting period,hence we termed them hypsophyllary buds. In trees with nakedbuds, immature leaves resumed their growth and developed tomature leaves the following spring; thus these trees had nospecial organs to cover shoot tips during winter. In trees withhypsophyllary buds, some hypsophylls covering the shoot tipsthrough the year were shed without further growth when new shootsstarted to grow in the spring. In trees with scaled buds, newlygrowing shoots had hypsophyllary buds at their tips in spring.After the completion of stem elongation, the buds were replacedby scaled buds (often covered with more than 30 scales) in summer.These scaled buds grew during autumn and winter until a newflush of growth the following spring. The three bud types correspondedto forest stratification in the northern-limit forest: the nakedbuds of Rubiaceae and Myrsinaceae in the ground layer; the hypsophyllarybuds of various families (e.g. Symplocaceae, Myrsinaceae) inthe understorey; and the scaled buds of Fagaceae and Lauraceaein the forest canopy. The position and activity of buds on abranch were reflected in the architectural patterns of the treesin different layers of the forest. The scaled-bud trees hadwell-protected, abundant axillary buds and are probably suitedto survive in the forest canopy (with frequent disturbances),whereas the single terminal bud of hypsophyllary-bud trees cansurvive in the less disturbed, resource-limited understoreyof the forest.Copyright 1998 Annals of Botany Company Bud structural type; bud formation; bud growth; shoot elongation; shoot-growth cycle; branching architecture; forest stratification. 相似文献
11.
Background and Aims: Recent studies have shown that small structures on plant surfacesserve ecological functions such as resistance against herbivores.The morphology, distribution, chemical composition and changesduring shoot and leaf development of such small structures wereexamined on Paulownia tomentosa. Methods: The morphology and distribution of the structures were studiedunder light microscopy, and their chemical composition was analysedusing thin-layer chromatography and high-performance liquidchromatography. To further investigate the function of thesestructures, several simple field experiments and observationswere also conducted. Key Results: Three types of small structures on P. tomentosa were investigated:bowl-shaped organs, glandular hairs and dendritic trichomes.The bowl-shaped organs were densely aggregated on the leavesnear flower buds and were determined to be extrafloral nectarines(EFNs) that secrete sugar and attract ants. Nectar productionof these organs was increased by artificial damage to the leaves,suggesting an anti-herbivore function through symbiosis withants. Glandular hairs were found on the surfaces of young and/orreproductive organs. Glandular hairs on leaves, stems and flowerssecreted mucilage containing glycerides and trapped small insects.Secretions from glandular hairs on flowers and immature fruitscontained flavonoids, which may provide protection against someherbivores. Yellow dendritic trichomes on the adaxial side ofleaves also contained flavonoids identical to those secretedby the glandular hairs on fruits and flowers. Three specialtypes of leaves, which differed from the standard leaves inshape, size and identity of small structures, developed nearyoung shoot tips or young flower buds. The density of smallstructures on these leaf types was higher than on standard leaves,suggesting that these leaf types may be specialized to protectyoung leaves or reproductive organs. Changes in the small structuresduring leaf development suggested that leaves of P. tomentosaare primarily protected by glandular hairs and dendritic trichomesat young stages and by the EFNs at mature stages. Conclusions: The results indicate that P. tomentosa protects young and/orreproductive organs from herbivores through the distributionand allocation of small structures, the nature of which dependson the developmental stage of leaves and shoots. 相似文献
12.
Tuberization in Potato at High Temperatures: Gibberellin Content and Transport from Buds 总被引:1,自引:0,他引:1
Warm temperatures (35°C day/30°C night) which inhibittuberization in potato (Solanum tuberosum L., cv. Sebago) increasedgibberellin activity in crude extracts from buds, but not frommature leaves, as determined by the lettuce hypocotyl bioassay.Changes in the growth of tubers and stolons indicate the occurrenceof basipetal movement of GA3 applied to the terminal bud ora mature leaf. 14C labelling from GA3 or mevalonic acid injectedjust below the terminal bud was recovered in the lower shoot,stolons and tubers, but the amount transported was greater atcool temperatures (20/15°C). It is concluded that high temperaturespromote the synthesis of gibberellin in the buds rather thantransport to the stolons. Solanum tuberosum L., potato, tuberization, gibberellin 相似文献
13.
Hongyan Dai Zhihong Zhang Xiuwu Guo 《In vitro cellular & developmental biology. Plant》2007,43(1):2-8
Hawthorn (Crataegus spp.) is an important plant with a long history as an ornamental and a source of medicine. A protocol is outlined for adventitious
bud regeneration from leaf and cotyledon explants of Chinese hawthorn (C. pinnatifida Bge. var. major N.E.Br.). Adventitious buds were induced on both the leaves of sprouting winter buds and the leaves of in vitro plants, but the percentage of bud regeneration from leaves of in vitro plants was very low—less than 6%. On N6 medium supplemented with 31.08 μM BA and 9.67 μM NAA, the percentages of bud regeneration
from leaves of sprouting winter buds of cultivars “Liaohong” and “Qiujinxing” were 31.4% and 17.6%, respectively. The regeneration
abilities of three kinds of cotyledon explants, immature cotyledon, mature cotyledon, and cotyledon leaf, were compared. The
percentage of bud regeneration from cotyledon leaves was higher. On MS media supplemented with 4.44 μM BA and 4.54–9.08 μM
TDZ, the percentages of bud regeneration from cotyledon leaves of cultivars “Qiujinxing” and “Xiajinxing” were 27.7 ± 7.8%
and 20.1 ± 4.7%, respectively, and the numbers of buds per explant were 5.9 ± 1.6 and 3.2 ± 0.7, respectively. On B5 medium
supplemented with 2.22 μM BA, 2.32 μM Kn, and 0.57 μM IAA, adventitious buds grew quickly and 80–100% of buds developed into
shoots. The shoots rooted successfully with the two-step rooting method. Ninety days after transplantation, more than 80%
plants were survived. This system of adventitious bud regeneration from leaf and cotyledon explants could be useful for the
genetic transformation and polyploidization of Chinese hawthorn. 相似文献
14.
Bud Structure in Relation to Shoot Morphology and Position on the Vegetative Annual Shoots of Juglans regia L. (Juglandaceae) 总被引:2,自引:0,他引:2
The length and basal diameter of all lateral and terminal budsof vegetative annual shoots of 7-year-oldJuglans regia treeswere measured. All buds were dissected and numbers of cataphylls,embryonic leaves and leaf primordia were recorded. Each axillarybud was ranked according to the position of its associated leaffrom the apex to the base of its parent shoot. Bud size andcontent were analysed in relation to bud position and were comparedwith the size and number of leaves of shoots in equivalent positionswhich extended during the following growing season. Length andbasal diameter of axillary buds varied according to their positionon the parent shoot. Terminal buds contained more embryonicleaves than any axillary bud. The number of leaves was smallerfor apical and basal axillary buds than for buds in intermediatepositions on the parent shoot only. All new extended shootswere entirely preformed in the buds that gave rise to them.Lateral shoots were formed in the median part of the parentshoot. These lateral shoots derived from buds which were largerthan both apical and basal ones. Copyright 2001 Annals of BotanyCompany Juglans regia L., Persian walnut tree, branching pattern, preformation, bud content, shoot morphology 相似文献
15.
Growth Context and Fate of Axillary Meristems of Young Peach Trees. Influence of Parent Shoot Growth Characteristics and of Emergence Date 总被引:1,自引:0,他引:1
The relationship between several growth components of a shootand the fates of the axillary meristems (developing in the axilsof the leaves) borne by that shoot were studied, on first-ordershoots of young peach trees. A comprehensive picture of thoserelationships was obtained by a discriminant analysis. Shootgrowth at meristem emergence date was characterized by internodelength, leaf-production rate and leaf-unfolding duration. Allpossible fates of axillary meristems at the end of the growingseason (i.e. blind nodes, single vegetative or flower bud, budassociations, sylleptic or proleptic shoots) were considered.Shoot-elongation rate determined meristem fates quantitatively.The number of buds produced by a meristem increased when theshoot-elongation rate increased. Qualitatively, the fate of axillary meristems was related tothe balance between shoot-growth components. If the subtendingleaf unfolded slowly, sylleptic or proleptic shoots were morelikely to develop than bud associations, for high shoot-elongationrates; and flower buds were more frequent than vegetative buds,for low shoot-elongation rates. Compared to flower buds, blindnodes appeared for similar shoot-elongation rates but longerinternodes and lower leaf-production rates. The emergence dateslightly modified the relation between shoot growth and axillary-meristemfates, but the main features held true throughout the growingseason. The relationships between shoot growth and meristem fates mayresult from competitive interactions between the growing subtendingleaf and the developing axillary meristem. Growing conditionsmight also influence both shoot growth and meristem fates byfavouring either cell enlargement or cell division.Copyright1995, 1999 Academic Press Peach tree, Prunus persica (L.) Batsch, axillary meristem, meristem fate, branching, flowering, shoot growth, discriminant analysis, exploratory analysis 相似文献
16.
Direct plant regeneration from leaf explants of Plumbago species and its genetic fidelity through RAPD markers 总被引:1,自引:0,他引:1
G R ROUT 《The Annals of applied biology》2002,140(3):305-313
In vitro plant regeneration was achieved from leaf explants of Plumbago rosea and Plumbago zeylanica on Murashige & Skoog (1962) medium supplemented with 1.5 mg litre?1 6‐benzylaminopurine, 0.25 mg litre?1 indole‐3‐acetic acid, 50 mg litre?1 adenine sulfate and 3% (w/v) sucrose. The shoot initials developed within 2–3 wk on the leaf margin as well as from the wounds of the leaf. High frequency shoot‐bud regeneration was achieved on similar medium in subsequent subcultures. The semi‐mature leaves produced more shoot‐buds as compared to the younger leaves. Mature leaves did not show any response for shoot bud initiation. More than 85% of the semi‐mature explants produced shoot‐buds per leaf explant within 4 wk of culture. Shoots rooted easily on medium having half‐strength basal Murashige & Skoog (1962) medium supplemented with 0.25 mg litre?1 indole‐3‐butyric acid and 2% (w/v) sucrose; 84–92% of the in vitro rooted plantlets survived in the greenhouse. The regenerated plantlets appeared morphologically similar to the mother plants. No variation was detected among the regenerated plants by the use of Randomly Amplified Polymorphic DNA (RAPD) markers. This method might be useful for assessing plant improvement programmes. 相似文献
17.
Dry Matter Assimilation and Partitioning in Pepper Cultivars Differing in Susceptibility to Stress-induced Bud and Flower Abscission 总被引:1,自引:0,他引:1
Stress-induced abscission of buds and flowers is a constraintto the production of peppers (Capsicum annuum L.). Susceptibilityof pepper cultivars to stress-induced abscission may be relatedto assimilate levels and partitioning. Growth analysis experimentswere conducted in the field to determine whether cultivars differingin susceptibility to stress-induced abscission showed correspondingdifferences in growth rates and dry matter partitioning whensubjected to low light stress. With the stress susceptible cultivar'Shamrock', reduction in net assimilation rate (NAR) and relativegrowth rate (RGR) was greater under low light stress than withthe more tolerant cultivar 'Ace'. Compared to 'Ace', 'Shamrock'partitioned a smaller proportion of dry matter into reproductiveorgans and a larger proportion into expanded leaves. Cultivarsdid not differ in the amount of dry matter partitioned to youngleaves or in the extent to which accumulation of dry matterby young leaves was reduced by low light stress. Other experimentsconducted in growth chambers and greenhouse examined the effectof removal of leaves less than 3 cm long on high temperatureand low light stress-induced bud and flower abscission. Forboth 'Ace' and 'Shamrock', abscission was not altered by removalof these leaves. Preferential partitioning of assimilate toyoung leaves does not appear to be involved in stress-inducedabscission in these pepper cultivars.Copyright 1994, 1999 AcademicPress Pepper (Capsicum annuum L.), abscission, low light stress, high temperature stress, assimilate partitioning, assimilation/growth rate, growth analysis, defoliation 相似文献
18.
The influence of the shoot apex upon leaf and bud formationin the fern Dryopteris aristata has been investigated by furtherexperiments on puncturing the apical cell. When the apical cellgroup is damaged, leaf primordia, which may be orientated abnormally,continue to be formed on the meristem, but one or more budsmay also arise. The observations reported here indicate thata zone at the periphery of the apical meristem is particularlyreactive when the apical cell group is damaged, the majorityof buds being induced in this region. The extent of damage tothe apex may affect the sequence of organogenesis: when damageis extensive buds tend to be formed immediately, subsequentprimordia developing as leaves; when the damage is confinedto the apical cell, or extends to only a few of its segments,bud formation tends to be delayed. It is concluded that the effect of the apical cell on organformation is exercised through the growth and organization ofthe apex as a whole. 相似文献
19.
Buds of sweet orange, harvested from shoots of different timeof flushing and from different positions along the shoot, wereused to examine whether lack of burst of inserted buds was acharacteristic of the bud. Bursting of inserted buds was significantlyslower in buds taken from (a) older branches (b) shoots producedunder winter conditions, and (c) basal rather than apical budson the same shoot. The slowness to burst when transferred matched a tendency todormancy in buds on shoot segments grown in vitro, suggestingthat the variation in budburst was intrinsic to the bud. Budburstwas correlated with the extent of secondary bud development;the majority of buds from apical regions of the shoot had developeda secondary bud by the time of implantation, but basal budshad not. Adequate vascular connections with the host tissueswere found in both burst and unburst buds. Citrus sinensis (L.) Osbeck, sweet orange, buds, endodormancy, budding 相似文献
20.
It was postulated that the release of lateral buds from apical dominance is triggered by the immediate increase in apoplastic water potential (hydrostatic pressure) that is produced by shoot decapitation and that is rapidly transmitted throughout the plant. In experiments conducted to test this hypothesis the use of a strain gauge transducer capable of measuring bud growth with an accuracy of ± 0.1 μm, showed that growth of the inhibited lateral bud at the primary leaf node of Phaseolus vulgaris (L.) ev. Canadian Wonder was initiated within 1 to 5 s following shoot decapitation or excision of the primary leaves. When only the apical bud was excised the lateral bud showed a brief, transitory growth response of ca 1 min duration, but the axillary buds of the first and second trifoliate leaves were released from inhibition. Decapitation of the shoot just below the first trifoliate leaf induced a lateral bud response characterized by three distinct stages: a) a rapid initial growth response with a mean duration of 4.9 min b) a period of arrested growth, which varied in duration from 2 min to 4 h and c) the subsequent resumption of growth.
Excision of both primary leaves induced a rapid but transitory bud response of considerably greater duration than that induced by apical bud excision. Excision of the primary leaves prior to decapitation of the shoot eliminated the phase of arrested growth, which characterized the bud response to decapitation of the intact plant. The rapidity of the bud response to both shoot decapitation and leaf excision and the interaction between the effect of these two treatments are consistent with the hypothesis that competition for water plays a major role in the correlative inhibition of lateral buds. 相似文献
Excision of both primary leaves induced a rapid but transitory bud response of considerably greater duration than that induced by apical bud excision. Excision of the primary leaves prior to decapitation of the shoot eliminated the phase of arrested growth, which characterized the bud response to decapitation of the intact plant. The rapidity of the bud response to both shoot decapitation and leaf excision and the interaction between the effect of these two treatments are consistent with the hypothesis that competition for water plays a major role in the correlative inhibition of lateral buds. 相似文献