共查询到20条相似文献,搜索用时 31 毫秒
1.
Yuriy Shckorbatov Vladimir Pasiuga Nicolai Kolchigin Dmitry Batrakov Oleg Kazansky Vladimir Kalashnikov 《Central European Journal of Biology》2009,4(1):97-106
The effects of ultra wideband pulse radiation on human cells were investigated. The density of the flow of energy on the surface
of irradiated object varied from 10−6 to 10−2 W/cm2 with exposure of 10 s. It was shown that heterochromatin granule quantity in cell nuclei increased under the influence of
radiation from 10−4 to 10−2 W/cm2. In some intervals the effect increased with irradiation dose. At irradiation intensity 10−3 W/cm2 the process of heterochromatin granule formation was fully reversible after 2 h of recovery; at intensity 10−2 W/cm2 the reversion of irradiation effects was not full. The data obtained indicated the strong biological activity of ultra wideband
ultra short pulse radiation. 相似文献
2.
O. G. Kulikova V. P. Yamskova A. P. Il’ina D. V. Margasyuk A. A. Molyavka I. A. Yamskov 《Applied Biochemistry and Microbiology》2011,47(4):356-360
A bioregulator that has physicochemical and biological properties similar to a group of bioregulators isolated from various
animal tissues has been found in the bulb onion (Allium cepa L.). It was determined that the biological action of the plant bioregulator is determined by a peptide with molecular weight
of 4036 ± 2 Da whose 18-C-terminal amino acid sequence consisted of 18 residues. On models of seed germination of some vegetable
cultures, the ability of the bioregulator isolated from supernatant of onion extract in ultralow doses (10−13 mg of protein/ml) to inhibit growth and development was demonstrated. 相似文献
3.
Charles O. Olaiya 《Journal of Plant Growth Regulation》2010,29(3):349-356
The bioregulators indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA) were investigated
for their effects on seedling growth and yield of tomato (Solanum lycopersicon). Seeds of tomato genotypes NHLy11, NHLy12, NHLy13, NHLy15 and NHLy16 were subjected to presowing treatments in 25-, 50-,
75-, 100-, 125-, and 150-mg/l concentrations of each of the bioregulators and control replicated three times in a completely
randomized design. Results revealed that IAA and IBA significantly retard (P < 0.05) the growth of radicles and hypocotyls of all test genotypes relative to controls, especially at the high concentrations
of 125- and 150-mg/l solutions of the bioregulators. NAA treatments enhanced seedling growth moderately at concentrations
up to 100 mg/l. At 125- and 150-mg/l concentrations, the seedlings showed reduced radicle and hypocotyl lengths compared with
those of controls. All treated genotypes had higher average fruit weight and mean fruit yield than controls at the 100-mg/l
concentration of the bioregulators. IAA had the highest yield of 25,900 kg fw ha−1 in the NHLy12 genotype, whereas the lowest yield of 3,760 kg fw ha−1 was obtained in the IBA-treated NHLy16 genotype. This study showed that presowing seed treatments with IAA, IBA, and NAA
were effective in enhancing seedling growth and yield in tomato, especially at the 100-mg/l concentration. 相似文献
4.
Conte da Frota ML Gomes da Silva E Behr GA Roberto de Oliveira M Dal-Pizzol F Klamt F Moreira JC 《Molecular and cellular biochemistry》2006,285(1-2):173-179
In this work we investigated the effects of retinoic acid (RA) in Sertoli cells. Sertoli cells isolated from 15-day-old Wistar rats were previously cultured for 48 h and then treated with RA for 24 h. RA at high doses (1–10 μM) increased TBARS levels and induced a decrease in cell viability. At low doses (0.1–100 nM) RA did not increase TBARS level. RA also did not increase cell death at these doses. In order to investigate changes in antioxidant defenses we measured the CAT, SOD and GPx activities in Sertoli cells treated with RA. Compared to control, RA increased around 200% SOD activity in all doses tested (0.1–100 nM); GPx activity was increased 407.49, 208.98 and 243.88% (0.1, 1 and 10 nM, respectively); CAT activity was increased 127% with RA 1 nM. To clarify if RA induces ROS production per se, we performed experiments in vitro using 2-deoxyribose as specific substrate of oxidative degradation by •OH radical as well as TRAP assay. RA at 10 μM increased 2-deoxyribose degradation, suggesting that some of the RA-induced effects are mediated via •OH formation. Furthermore, the total reactive antioxidant potential (TRAP) of the RA was determined. At low concentrations RA has induced no redox activity. Conversely, higher concentration of RA (1–10 μM) increased chemiluminescence. The chemiluminescence produced was directly proportional to radical generation. We provide, for the first time, evidence for a free radical generation by RA. Our results demonstrated that RA plays an important role in Sertoli cells and these effects appear to be mediated by ROS. 相似文献
5.
Colette Briançon Sylvain Halpern Josette Jeusset Philippe Fragu 《Biological trace element research》1992,32(1-3):267-273
Analytical ion microscopy (AIM) was used to determine alterations in the thyroid follicular lumen127I stores of Wistar rats injected with different doses of129I (low specific activity radionuclide). Animals fed a normal iodine diet (10 μg127I/d) were divided into four groups: control group and three treated groups injected ip 24 h before sacrifice with129I at doses of 10 μg (group 1), 30 μg (group 2), and 8500 μg (group 3). AIM was performed on thyroid semithin sections. The
mean129I concentration increased with the dose injected from group 1 (0.44±0.03 μg/mg, mean ± SEM) to group 2 (2.05±0.14 μg/mg) and
decreased in group 3 (0.57±0.08 μg/mg). When compared to control group (4.14±0.17 μg/mg), the mean127I concentration was not changed in group 1 (4.52±0.07 μg/mg), but altered in the other groups: It was significantly increased
(7.14±0.41 μg/mg) in group 2 and slightly decreased (3.11±0.26 μg/mg) in group 3. These results underline the interest of
AIM in the study of the effects of various doses of iodide on the thyroid autoregulation by iodide, a trace element actively
trapped by this gland. 相似文献
6.
Yuriy Shckorbatov Irina Rudneva Vladimir Pasiuga Valentin Grabina Nikolay Kolchigin Dmitriy Ivanchenko Oleg Kazanskiy Valentin Shayda Oleksandr Dumin 《Central European Journal of Biology》2010,5(6):785-790
The influence of magnetic fields on hatching and chromatin state of brine shrimp, Artemia sp., was investigated. Dry Artemia cysts were exposed to a magnetic field of intensity 25 mT for 10 min. The magnetic field was applied in different variants:
constant field, rotating field of different directions (right-handed and left-handed) and different magnet polarization. The
effect of ultra wideband pulse radiation and microwave radiation was also investigated. The energy density on the surface
of object exposed to ultra wideband pulse radiation was 10−2, 10−3, 10−4, 10−5 and 10−6 W/cm2, the power of microwave radiation was 10−4 and 10−5 W/cm2, exposure time - 10 s. After incubation of the cysts for 48 hours in sea water the hatching percentage of Artemia from exposed cysts was higher than in controls. The number of heterochromatin granules was significantly higher in the nauplia
(newborn larvae of Artemia) developed from cysts that had been exposed to magnetic and electromagnetic fields. The data obtained demonstrate an increase
in percentage hatching of Artemia cysts after treatment with magnetic and electromagnetic fields and chromatin condensation in nauplia. We have also shown
different effects of right-handed and left-handed rotating magnetic fields on these processes. 相似文献
7.
Globally, water deficit is one of the major constraints in chickpea (Cicer arietinum L.) production due to substantial reduction in photosynthesis. Photorespiration often enhances under stress thereby protecting the photosynthetic apparatus from photoinhibition. Application of bioregulators is an alternative to counter adverse effects of water stress. Thus, in order to analyze the role of bioregulators in protecting the photosynthetic machinery under water stress, we performed an experiment with two contrasting chickpea varieties, i.e., Pusa 362 (Desi type) and Pusa 1108 (Kabuli type). Water deficit stress was imposed at the vegetative stage by withholding water. Just prior to exposure to water stress, plants were pretreated with thiourea (1,000 mg L?1), benzyladenine (40 mg L?1), and thidiazuron (10 mg L?1). Imposed water deficit decreased relative water content (RWC), photosynthetic rate (PN), quantum efficiency of PSII (Fv/Fm), and enhanced lipid peroxidation (LPO). However, bioregulator application maintained higher RWC, PN, Fv/Fm, and lowered LPO under water stress. Expression of Rubisco large subunit gene (RbcL) was low under water stress both in the Kabuli and Desi type. However, bioregulators strongly induced its expression. Although poor expression of two important photorespiratory genes, i.e., glycolate oxidase and glycine decarboxylase H subunit, was observed in Desi chickpea under imposed stress, bioregulators in general and cytokinins in particular strongly induced their expression. This depicts that the application of bioregulators protected the photosynthetic machinery by inducing the expression of RbcL and photorespiratory genes during water deficit stress. 相似文献
8.
Agnieszka Mostowska 《Acta Physiologiae Plantarum》1998,20(4):419-424
Effect of low (5 mmol·dm−3) and high (10 or 20 mmol·dm−3) doses of 1.10-phenanthroline (Phe), a photodynamic herbicide, on the development of chloroplasts in etiolated and subsequently
illuminated maize seedlings and on the structure of already developed chloroplasts of green maize seedlings was examined.
Etiolated and then irradiated plants were resistant to 5 mmol·dm−3 of Phe with respect to morphology, however Phe caused inhibition of greening and of grana formation. Higher Phe concentrations
followed by exposure to light caused not only total inhibition of greening but also dilation of thylakoids, swelling of chloroplasts,
and finally total destruction of chloroplast structure. Application of Phe in the same concentrations to green plants revealed
that they were resistant to low dose of Phe with respect to morphology and structure of chloroplasts, however 10 and 20 mmol·dm−3 Phe and illumination caused the loss of turgor of treated plants and other photooxidative damages seen at the ultrastructural
level. We concluded that maize, as representant of monocotyledonous plants, is resistant to low (5 mmol·dm−3) Phe concentration. Higher (10 or 20 mmol·dm−3) concentrations, used to determine the site of damage and mode of action of Phe on the level of cell revealed that action
of photodynamic herbicides is based on standard photoinhibition mechanism and also probably on their chelating properties. 相似文献
9.
Wester RC Hartway T Maibach HI Schell MJ Northington DJ Culver BD Strong PL 《Biological trace element research》1998,66(1-3):111-120
Literature from the first half of this century reports concern for toxicity from topical use of boric acid, but assessment
of percutaneous absorption has been impaired by lack of analytical sensitivity. Analytical methods in this study included
inductively coupled plasmamass spectrometry which now allows quantitation of percutaneous absorption of10B in10B-enriched boric acid, borax and disodium octaborate tetrahydrate (DOT) in biological matrices.
In vitro human skin percent doses of boric acid absorbed were 1.2 for a 0.05% solution, 0.28 for a 0.5% solution, and 0.70
for a 5.0% solution. These absorption amounts translated into flux values of, respectively, 0.25, 0.58, and 14.58 μg/cm2/h, and permeability constants (K
p
) of 5.0 x 10-4, 1.2 x 10-4, and 2.9 x 10-4 cm/h for the 0.05%, 0.5%, and 5.0% solutions. The above in vitro doses were at infinite, 1000 μL/cm2 volume. At 2 μL/cm2 (the in vivo dosing volume), flux decreased some 200-fold to 0.07 μg/cm2/h andK
p
of 1.4 x 10-6 cm/h, while percent dose absorbed was 1.75%.
Borax dosed at 5.0%/1000 μL/cm2 had 0.41 percent dose absorbed, flux at 8.5 μg/cm2/h, andK
p
was 1.7 x 10-4 cm/h. Disodium octaborate tetrahydrate (DOT) dosed at 10%/1000 μL/cm2 was 0.19 percent dose absorbed, flux at 7.9 μg/cm2/h, andK
p
was 0.8 x ICH cm/h. These in vitro results from infinite doses (1000 μL/cm2) were a 1000-fold greater than those obtained in the companion in vivo study. The results from the finite (2 μL/cm2) dosing were closer (10-fold difference) to the in vivo results. General application of infinite dose percutaneous absorption
values for risk assessment is questioned by these results. 相似文献
10.
Jaleh Daie 《Plant Growth Regulation》1987,5(3):219-228
The sink mobilizing abillity is partially determined by sugar uptake rates of storage cells. Two synthetic growth regulators (Pix and BAS 106W) were tested for their effect on sucrose uptake in root tissue discs or glucose uptake in cell cultures of sugar beet. In tissue discs, uptake at the plasmalemma was determined by incubating the discs for 1 h in the presence of 5 mM sucrose and at the tonoplast for 4 h in the presence of 40 mM sucrose. Cell cultures were incubated for 1 h in the presence of 1 mM glucose. Pix (10 mg l–1) caused a 20% stimulation of active sucrose uptake at the plasmalemma. Active sucrose uptake at the tonoplast was increased 67% by 100 mg l–1 Pix. No effect of BAS 106W was observed on sucrose uptake in tissue discs. In cell cultures, a 65% enhancement of active glucose uptake was observed with both Pix and BAs 106W. When the bioregulators were applied to the root medium of seedlings, Pix but not BAS 106W resulted in increased root/shoot ratio, translocation of 14C-assimilates, and allocation of more biomass to the root sink. The data suggested that sugar transport and translocation may be used as biochemical criteria for rapid screening of effective yield enhancing bioregulators. 相似文献
11.
Pretreatment ofVicia faba root tip cells with low doses of60Co γ-radiation can reduce the yield of micronuclei induced by a subsequent high dose of γ-radiation (1.5 Gy). This adaptation
to γ-radiation possesses the following characteristics: (1) this phenomenon can be induced by an optimal range of low doses of γ-radiation applied acutely or fractionally; (2) the expression of this response seems to be transitory and depends on the time interval between adapting and challenging
doses of γ-radiation.
Communicated by T. GICHNER 相似文献
12.
Summary Short-term culture of rainbow trout (Onchorhynchus mykiss) hepatocytes was used to examine the effect of dexamethasone (DEX) on microsomal CYP 1A1 protein content and 7-ethoxyresorufin-O-deethylase (EROD) activity in vitro. Hepatocytes prepared by controlled collagenase digestion and plated at a density of
0.25 × 106 cells/cm2 in plastic culture dishes precoated with trout skin extract (7.6 μg skin protein/cm2) to facilitate cell attachment were maintained at 16° C. Cells were treated with DEX (10−9 to 10−7
M) or vehicle (dimethyl sulfoxide, DMSO) at 24 h. Microsomal CYP 1A1 protein content and EROD activities were measured at 72
h. Both CYP 1A1 protein as measured by Western blots using CYP 1A1 specific anti-sera and EROD activity were significantly
lower in DEX (10−8 to 10−7
M)-treated hepatocytes compared to untreated (control) or DMSO-treated cells. The effect was dose dependent in that a gradual
decrease of CYP 1A1 protein and EROD activities were seen with increasing doses of DEX (10−8 to 10−7
M). DEX at 10−9
M was ineffective. Concomitant addition of 10−6
M RU486, a type II specific glucocorticoid receptor antagonist, to hepatocytes treated with 10−7
M DEX abolished the DEX effect. RU486 at 10−8
M was ineffective. Spironolactone (10−8 to 10−6
M), a type I specific glucocorticoid receptor antagonist, did not counteract the DEX effect. RU486 or spironolactone (10−6
M) alone had no effect on CYP 1A1 under similar conditions. DEX thus down regulates CYP 1A1 in fish cultured hepatocytes and
this regulation is mediated through the type II glucocorticoid receptor(s). 相似文献
13.
Conidia of Beauveria bassiana and Metarhizium spp. smeared on glass slides were assayed for their responses to irradiation with weighted 312-nm UV-B and 365-nm UV-A at
gradient doses of 0.005–1.1 and 1.0–18.0 J cm−2, respectively. All inverted, sigmoid dose–survival trends showed good fit to a survival model (r
2 ≥ 0.97), yielding respective UV-B LD50s of 0.23–0.59 and 0.05–0.65 J cm−2 for 24 B. bassiana and 36 Metarhizium isolates, and UV-A LD50s of 2.78–10.46 J cm−2 for 24 Metarhizium isolates. Myzus persicae apterae on detached leaves were sprayed with a concentrated spore suspension of B. bassiana or M. anisopliae, followed by exposure to the UV-B doses to cause 10–90% viability losses. These doses caused aphid mortality reductions as
expected but affected neither spray-to-death period nor fungal growth on cadavers. The results highlight the merits of using
UV-tolerant candidates and photoprotection measures in fungal formulations for pest control. 相似文献
14.
We treated cultured tobacco leaf segments with brassinolide (BL) and naphthalene acetic acid (NAA) and determined that optimum
concentrations of NAA for adventitious root, trichome-like root, and calli formation were, respectively, 10−6, 10−5, and 10−4 M. In the adventitious root formation group, the number and length of adventitious roots were increased at lower concentrations
of BL; however, they became trichome-like roots at higher levels of BL. The trichome-like root formation group showed better
development when a low concentration of BL was added. However, at higher concentrations of BL, trichome-like root production
was reduced, forming calli instead. In the calli formation group, more calli were formed at low BL concentrations and after
persistent exposure to BL regardless of BL concentration, and the size of the leaf segments increased. The CNT103 gene, which is expressed at the root tips showed increased levels of expression at BL concentrations up to 10−9 M and decreased levels of expression at BL concentrations over 10−9 M in the adventitious roots, trichome-like roots, and calli formation groups. 相似文献
15.
Richard C. Baybutt Brendon W. Smith Elena V. Donskaya Ling Hu Ting Li Weiqun Wang 《In vitro cellular & developmental biology. Animal》2010,46(1):20-27
Retinoic acid (RA) is important for maintaining integrity of alveolar epithelial cells, but the mechanism has not been defined.
We cultured type II pneumocytes at confluent, high cell density (104 cells/mm2) and found that RA (10−6 M) inhibited thymidine incorporation to 60% of control, despite a dose-dependent increase in epidermal growth factor receptor
(EGFR) levels. However, at lower, subconfluent density (102 cells/mm2), RA stimulated thymidine incorporation to 280% of control. EGF increased thymidine incorporation at concentrations as low
as 0.1 ng/mL, but no further increase was observed at higher concentrations up to 100 ng/mL. In subconfluent cells co-treated
with EGF (100 ng/mL) and increasing concentrations of RA (10−8 M–10−5 M RA), thymidine incorporation was significantly greater at all concentrations than RA alone, with greatest increases observed
at 10−7 (422% of control) and 10−6 (470% of control) M RA. In summary, the effects of RA on thymidine incorporation are sensitive to changes in cell density.
RA inhibits thymidine incorporation at high cell density and stimulates thymidine incorporation at low density. RA increases
EGFRs in cultured type II pneumocytes, and EGF stimulates thymidine incorporation independent of the cultured cell density.
These data may help to explain how RA mediates lung repair in vivo. 相似文献
16.
Aldegunde M Andrés MD Soengas JL 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2000,170(3):237-243
The influx of glucose into the brain and plasma glucose disappearance were estimated in rainbow trout (Oncorhynchus mykiss) intravenously injected (1 ml · kg−1 body weight) with a single dose (15 μCi · kg−1 body weight) of 3-O-methyl-D-[U-14C]glucose ([U-14C]-3-OMG) at different times (2–160 min), and after intravenous injection at 15 min of increased doses (10–60 μCi · kg−1 body weight) of [U-14C]-3-OMG. Brain and plasma radiotracer concentrations were measured, and several kinetic parameters were calculated. The apparent
brain glucose influx showed a maximum after 15–20 min of injection then decreased to a plateau after 80 min. Brain distribution
space of 3-OMG increased from 2 min to 20 min reaching equilibrium from that time onwards at a value of 0.14 ml · g−1. The unidirectional clearance of glucose from blood to brain (k1) and the fractional clearance of glucose from brain to blood (k2) were estimated to be 0.093 ml · min−1 · g−1, and 0.867 min−1, respectively. A linear increase was observed in brain and plasma radiotracer concentrations when increased doses of [U-14C]-3-OMG were used. All these findings support a facilitative transport of glucose through the blood-brain barrier of rainbow
trout with characteristics similar to those observed in mammals. The injection of different doses of melatonin (0.25–1.0 mg · kg−1) significantly increased brain glucose influx suggesting a possible role for melatonin in the regulation of glucose transport
into the brain.
Accepted: 26 January 2000 相似文献
17.
Hörig H Lee DS Conkright W Divito J Hasson H LaMare M Rivera A Park D Tine J Guito K Tsang KW Schlom J Kaufman HL 《Cancer immunology, immunotherapy : CII》2000,49(9):504-514
The generation of cytotoxic effector T cells requires delivery of two signals, one derived from a specific antigenic epitope
and one from a costimulatory molecule. A phase I clinical trial was conducted with a non-replicating canarypoxvirus (ALVAC)
constructed to express both human carcinoembryonic antigen (CEA) and the B7.1 costimulatory molecule. This was the first study
in cancer patients to determine if the delivery of costimulation with a tumor vaccine was feasible and improved immune responses.
Three cohorts of six patients, each with advanced CEA-expressing adenocarcinomas, were treated with increasing doses of an
ALVAC-CEA-B7.1 vaccine (4.5 × 106, 4.5 × 107, and 4.5 × 108 plaque-forming units, PFU). Patients were vaccinated by intramuscular injection every 4 weeks for 3 months and monitored
for side-effects, tumor growth and anti-CEA immune responses. ALVAC-CEA- B7.1 at doses up to 4.5 × 108 PFU was given without evidence of significant toxicity or autoimmune reactions. Three patients experienced clinically stable
disease that correlated with increasing CEA-specific precursor T cells, as shown by in vitro interferon-γ enzyme-linked immunoassay
spot tests (ELISPOT). These three patients underwent repeated vaccination resulting in augmented CEA-specific T cell responses.
This study represents the first use of costimulation to enhance antitumor vaccines in cancer patients. This approach resulted
in CEA-specific immunity associated with stable diseases in three patients. This study also demonstrated that CEA-specific
T cell responses could be sustained by repeated vaccinations. Although the number of patients was small, the addition of B7.1
to virus-based vaccines may improve immunological and stable diseases to vaccination against tumor-associated antigens with
tolerable toxicity.
Received: 6 May 2000 / Accepted: 13 July 2000 相似文献
18.
The effects of temperature and light on the growth and geosmin production of Lyngbya kuetzingii were determined. Of the three temperatures tested, 10, 25 and 35°C, the maximal geosmin concentration and geosmin productivity
were yielded at 10°C, while the highest chl a production was observed at 25°C. In the studies on light intensity, the maximal geosmin concentration and geosmin productivity
were observed at 10 μmol m−2 s−1, while the highest chl a production was at 20 μmol m−2 s−1. It was suggested that more geosmin was synthesized with lower chl a demand. Meanwhile, the relative amounts of extra- and intracellular geosmin were investigated. Under optimum growth conditions
(20 μmol m−2 s−1, 25°C; BG-11 medium), the amounts of extracellular geosmin increased as the growth progressed and reached the maximum in
the stationary phase, while the intracellular geosmin reached its maximum value in the late exponential phase, and then began
to decline. However, under the low temperature (10°C) or light (10 μmol m−2 s−1) conditions, more intracellular geosmin was synthesized and mainly accumulated in the cells. The proportions of extracellular
geosmin were high, to 33.33 and 32.27%, respectively, during the stationary phase at 35°C and 20 μmol m−2 s−1. It was indicated that low temperature or light could stimulate geosmin production and favor the accumulation of geosmin
in cells, while more intracellular geosmin may be released into the medium at higher temperatures or optimum light intensity. 相似文献
19.
Andrzej K. Siwicki Zdzisław Zakęś Elżbieta Terech-Majewska Krzysztof Kazuń Agnieszka Lepa Edward Głąbski 《Reviews in Fish Biology and Fisheries》2010,20(3):435-439
Influence of beta-1.3/1.6-glucan (Macrogard) on the innate immunity and protection against Aeromonas hydrophila in tench (Tinca tinca (L.)) was assessed. Macrogard was fed at doses of 0, 0.5, 1 and 2 g kg−1 of pellets for 1 month. The blood, spleen and head kidney from 10 fish of each group were separated and analysed for immunity
parameters. Twenty tench from each group were infected with A. hydrophila. Macrogard at doses 1 and 2 g kg−1 of feed significantly (P < 0.05) increased the phagocytic activity of macrophages and proliferative response of mitogens stimulated lymphocytes. The
same doses significantly (P < 0.05) increased lysozyme activity and Ig level in serum, compared to the control and dose 0.5 g kg−1 of feed. The challenge test showed that Macrogard reduced mortality of tench after experimental infection (5–35%). 相似文献
20.
Z. Katerova S. Ivanov E. Prinsen H. Van Onckelen V. Alexieva A. Azmi 《Biologia Plantarum》2009,53(2):365-368
Pea (Pisum sativum L., cv. Scinado) seedlings were exposed to low doses of ultraviolet-B (UV-B; 4.4 and 13.3 kJ m−2 d−1) or UV-C (0.1 and 0.3 kJ m−2 d−1) radiation for 14 d. Aminocyclopropane carboxylic acid (ACC), indoleacetic acid (IAA) and abscisic acid (ABA) contents were
quantified by gas chromatography coupled to mass spectrometry (GC-MS). The accumulation of ACC upon irradiation was dose-dependent.
ABA content was reduced and IAA content increased upon UV-C treatment whereas the UV-B doses used did not cause significant
changes in ABA and IAA contents. 相似文献