Effect of static magnetic field on some enzymes activities in rats

The magnetic field of 0.008 T and 0.15 T inductions influence lasting 7 weeks (7 days a week), 1 h daily determines the increase of the activity of cytoplasmatic enzymes (glutamic pyruvic transaminase, glutamic oxalacetic transaminase, lactic dehydrogenase), the decrease of cholinesterase activity and the growth of alkaline phosphatase activity in the plasma of the examined animals. The observed changes were reversible. 2 months after the exposure had been stopped, the tested parameters were back to normal.     

Helminthosporium maydis Race T Toxin Induces Leakage of NAD from T Cytoplasm Corn Mitochondria

The mechanism by which Helminthosporium maydis race T toxin inhibits respiration dependent on NAD⁺-linked substrates in T cytoplasm corn mitochondria was investigated. The toxin did not cause leakage of the soluble matrix enzyme malate dehydrogenase from the mitochondria or inhibit malate dehydrogenase or isocitrate dehydrogenase directly. The toxin did increase the permeability of the inner membranes of T cytoplasm, but not N cytoplasm, mitochondria to NAD⁺. Added NAD⁺ partially or fully restored toxin-inhibited electron transport in T cytoplasm mitochondria. Thiamin pyrophosphate had a similar effect when malate was the substrate. It was concluded that the inhibition of respiration of NAD⁺-linked substrates by the toxin is due to depletion of the intramitochondrial pool of NAD⁺ and other coenzymes.     

Thyroid thermogenesis in adult rat hepatocytes in primary monolayer culture: direct action of thyroid hormone in vitro

We have studied the effect of 3,5,3'-triiodothyronine (T3) on the respiration of adult rat hepatocytes in primary monolayer culture prepared from hypothyroid rat liver. After addition of T3 to the culture medium at a concentration of 2 x 10(-7) M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72--96 h, relative to control cultures (38.0 +/- 1.8 vs. 25.0 +/- 1.5 microliter/h.mg protein). The thyroid-responsive enzymes, Na+ + K+-activated adenosine triphosphatase (NaK-ATPase) and alpha-glycerophosphate dehydrogenase (GPD), each exhibited increased activity in response to T3, in parallel with the change in oxygen consumption, whereas the activity of Mg-dependent ATPase was unaffected. These responses to T3 were dose dependent over similar concentration ranges, the half-maximal response for each occurring at ca 8 x 10(-10) M. In thyroid-treated cells, the observed increase in respiration was almost completely (90%) inhibited after addition of ouabain (10(-3) M) to the culture medium. It was found also that a 4-h exposure of the cultured hepatocytes to T3 was sufficient to elicit a significant thermogenic response, measured at a time (48 h later) when T3 was no longer present in the medium. The response to T3 occurred in fully defined culture medium and was independent of the presence or absence of hypothyroid rat serum, corticosterone, or insulin, and cellular ATP was unaffected by T3 in concentrations up to 2 x 10(-7) M. The findings document that adult rat hepatocytes in primary monolayer culture respond directly to thyroid hormone; the increases in respiration and NaK-ATPase activity elicited by T3 were cotemporal and apparently coordinate.     

Effects Silver Nanoparticles and Magnetic Field on Growth of Fodder Maize (Zea mays L.)

Two experiments were done in 2008 and 2009 to study the effects of magnetic field and silver nanoparticles on fodder maize (Zea mays L.). These experiments were done with seven treatments based on a randomized complete block design in four replications. The treatments were as follows: magnetic field and silver nanoparticles?+?Kemira fertilizer (T1), magnetic field and silver nanoparticles?+?Humax fertilizer (T2), magnetic field and silver nanoparticles (T3), Kemira fertilizer (T4), Librel fertilizer (T5), Humax fertilizer (T6), and a control (T7). Results showed that fresh yield was higher in treatments T3 and T4. Treatments T3 and T4 had increased maize fresh yields of 35 and 17.5?% in comparison to the control, respectively. The dry matter yield of those plants exposed to magnetic field and silver nanoparticles was significantly higher than that from any of the other treatments. Magnetic field and silver nanoparticle treatments (T3 and T1) showed higher percentages for ears, and the lowest percentages were found in treatments T7 and T5. In general, the soil conditions for crop growth were more favorable in 2009 than in 2008, which caused the maize to respond better to treatments tested in the study; therefore, treatments had more significant effects on studied traits in 2008 than in 2009.     

Zn2+ inhibits alpha-ketoglutarate-stimulated mitochondrial respiration and the isolated alpha-ketoglutarate dehydrogenase complex

Intracellular free Zn(2+) is elevated in a variety of pathological conditions, including ischemia-reperfusion injury and Alzheimer's disease. Impairment of mitochondrial respiration is also associated with these pathological conditions. To test whether elevated Zn(2+) and impaired respiration might be linked, respiration of isolated rat liver mitochondria was measured after addition of Zn(2+). Zn(2+) inhibition (K(i)(app) = approximately 1 micrometer) was observed for respiration stimulated by alpha-ketoglutarate at concentrations well within the range of intracellular Zn(2+) reported for cultured hepatocytes. The bc(1) complex is inhibited by Zn(2+) (Link, T. A., and von Jagow, G. (1995) J. Biol. Chem. 270, 25001-25006). However, respiration stimulated by succinate (K(i)(app) = approximately 6 micrometer) was less sensitive to Zn(2+), indicating the existence of a mitochondrial target for Zn(2+) upstream from bc(1) complex. Purified pig heart alpha-ketoglutarate dehydrogenase complex was strongly inhibited by Zn(2+) (K(i)(app) = 0.37 +/- 0.05 micrometer). Glutamate dehydrogenase was more resistant (K(i)(app) = 6 micrometer), malate dehydrogenase was unaffected, and succinate dehydrogenase was stimulated by Zn(2+). Zn(2+) inhibition of alpha-ketoglutarate dehydrogenase complex required enzyme cycling and was reversed by EDTA. Reversibility was inversely related to the duration of exposure and the concentration of Zn(2+). Physiological free Zn(2+) may modulate hepatic mitochondrial respiration by reversible inhibition of the alpha-ketoglutarate dehydrogenase complex. In contrast, extreme or chronic elevation of intracellular Zn(2+) could contribute to persistent reductions in mitochondrial respiration that have been observed in Zn(2+)-rich diseased tissues.     

The effect of cell-phone radiation on rabbits: Lymphocyte enzyme-activity data

The effect of a GSM 900/1800 mobile phone, which is a widespread source of electromagnetic radiation of the microwave frequency in the environment, on rabbits was studied at power densities of 5–7 μW/cm². The biological effect was recorded by a sensitive method for the detection of the physiological regulation of enzyme activity inside lymphocytes in blood smears. Succinate dehydrogenase, which is the most powerful energy-supply enzyme in mitochondria, and lactate dehydrogenase, which is an enzyme of glycolysis, were measured. The lactate dehydrogenase to succinate dehydrogenase activity ratio was also calculated as an analog of the Warburg effect, which demonstrates the relationship between glycolysis and respiration. After 60 min of mobile-phone exposure each day for 11 days at a moderate dose, the emitted radiation induced a threefold increase in succinate dehydrogenase activity and a twofold decrease in lactate dehydrogenase activity. As a result, the lactate dehydrogenase/succinate dehydrogenase activity ratio falls from 15 to 5, thus indicating that respiration is predominant over glycolysis. The changes develop already after the first exposure and reach a maximum in 4 days. The predominance of respiration is usually considered as a beneficial state of an organism. However, continuous activation of respiration by mobile phone exposure may cause damage to the normal restorative processes that are supported by glycolysis during periods of rest.     

Effect of chronic exposure to static magnetic field upon the K+, Na+ and chlorides concentrations in the serum of guinea pigs

The magnetic field influence on the concentration of serum K+, Na+ and chlorides was tested. The guinea pigs were exposed to the static magnetic field for six weeks 1 hour a day, 7 days a week. Magnetic field of induction 0.005 T--0.3 T produced progressively an increase in Na+ concentration and a decrease in chlorides concentration in the serum. The range of observed changes was dependent on the duration of exposure to the magnetic field. No change in K+ serum concentration was observed following magnetic field exposure.     

Poliovirus induces an early impairment of mitochondrial function by inhibiting succinate dehydrogenase activity

Poliovirus infection of COS-1 and T47D cells caused a rapid decrease in total cell respiration, and this was attributed to an inhibition of mitochondrial respiration. The stimulation of mitochondrial respiration by pyruvate plus malate or succinate was impaired in saponin-permeabilised cells. However, this inhibition could be overcome by the addition of N,N,N',N'-tetramethyl-1, 4-phenylenediamine and ascorbate. The activity of succinate dehydrogenase was impaired in parallel with the inhibition of mitochondrial respiration during poliovirus infection. This shows that mitochondrial function is profoundly altered during poliovirus infection and that this occurs primarily through inhibition of electron flow at complex II of the mitochondrial respiratory chain.     

Origins of electromagnetic hypersensitivity to 60 Hz magnetic fields: A provocation study

With increasing electrical device usage, social concerns about the possible effects of 60 Hz electromagnetic fields on human health have increased. The number of people with self‐attributed electromagnetic hypersensitivity (EHS) who complain of various subjective symptoms such as headache and insomnia has also increased. However, it is unclear whether EHS results from physiological or other origins. In this double‐blinded study, we simultaneously investigated physiological changes (heart rate, respiration rate, and heart rate variability), subjective symptoms, and perception of the magnetic field to assess origins of the subjective symptoms. Two volunteer groups of 15 self‐reported EHS and 16 non‐EHS individuals were tested with exposure to sham and real (60 Hz, 12.5 µT) magnetic fields for 30 min. Magnetic field exposure did not have any effects on physiological parameters or eight subjective symptoms in either group. There was also no evidence that the EHS group perceived the magnetic field better than the non‐EHS group. In conclusion, the subjective symptoms did not result from the 60 Hz, 12.5 µT magnetic field exposures but from other non‐physiological factors. Bioelectromagnetics 33:326–333, 2012. © 2011 Wiley Periodicals, Inc.     

Environmental magnetic fields inhibit the antiproliferative action of tamoxifen and melatonin in a human breast cancer cell line

We have previously reported that environmental-level magnetic fields (1.2 μT [12 milligauss], 60 Hz) block the growth inhibition of the hormone melatonin (10⁻⁹ M) on MCF-7 human breast cancer cells in vitro. We now report that the same 1.2 μT, 60 Hz magnetic fields significantly block the growth inhibitory action of pharmacological levels of tamoxifen (10⁻⁷ M). In biophysical studies we have taken advantage of Faraday's Law of Current Induction and tested whether the 1.2 μT magnetic field or the associated induced electric field is responsible for this field effect on melatonin and tamoxifen. We observe that the magnetic field component is associated with the field blocking effect on melatonin and tamoxifen function. To our knowledge the tamoxifen studies represent the first experimental evidence for an environmental-level magnetic field modification of drug interaction with human breast cancer cells. Together, these findings provide support to the theory that environmental-level magnetic fields can act to modify the action of a drug or hormone on regulation of cell proliferation. Melatonin and tamoxifen may act through different biological pathways to down-regulate cell growth, and further studies are required to identify a specific biological site of interaction for the 1.2 μT magnetic field. Bioelectromagnetics 18:555–562, 1997. Published 1997 Wiley-Liss, Inc.

1 This article is a US Government work and, as such, is in the public domain in the United States of America.

     

Static Magnetic Field Influence on the Activity of some Respiratory Enzymes in Wheat

Most of the published studies on magnetic field action on biological systems have examined reactions in animals, while a smaller number of studies have reported magnetic field effects in plants. The effects of static magnetic field on the activity of several key enzymes in plant metabolism, such as malate dehydrogenase, succinate oxidase, succinate dehydrogenase, and cytochrome oxidase in young wheat seedlings, have been investigated in this study. It appears that the observed changes in enzyme activity could be considered to be a result of the influence of the magnetic field on the reactivity of these enzymes, including effects on metal cations that regulate enzyme activity. The results support the idea of the existence of “biological windows,” particularly with respect to exposure time.     

Effects of a Static Magnetic Field on Hemodynamics During Administration of a Cell Membrane Calcium Channel Blocking Agent

We investigated the influence of a 0.2 T static magnetic field (SMF) on the carotid sinus region in rabbits during infusion of a cell membrane calcium channel blocking agent (verapamil). The experiments were carried out under pentobarbital anesthesia. Blood pressure, heart rate, and respiration were also monitored. Contrary to our earlier studies without verapamil (1–4), the SMF had no significant effect on blood pressure and heart rate during verapamil infusion. Changes in calcium membrane channel transport might be participants in SMF effects on sinocarotid baro-receptors.     

The process of myelopoiesis in guinea pigs under conditions of a static magnetic field

Myelopoiesis and distribution of white blood cells in peripheral blood in 210 guinea pigs related to the duration and induction of an applied magnetic field were studied. Exposure to a static magnetic field of induction 0.05 T and 0.3 T each day for 7 weeks at 1 h per day led to a decrease of basophilic and polychromatophilic erythroblasts, an increase in the percentage of cells of the granulopoietic (neutrophilic myelocytes, eosinophilic metamyelocytes, band neutrophils, band basophils, segmented eosinophils) and lymphopoietic system, a slight increase in plasma cells and decrease in the percentage of megakaryocytes in bone-marrow. In the peripheral blood the percentage of lymphocytes was increased. The observed changes were independent on the duration of magnetic field action within the magnetic induction value.     

Effect of chronic exposure to static magnetic field upon the serum glutamic pyruvic transaminase activity GPT and morphology of the cardiac muscle, skeletal muscles, kidneys, cerebellum and lung tissue in guinea pigs

In a cycle of investigations concerning the pathogenesis of functional changes caused by the influence of magnetic field of induction occurring in laboratory and industrial conditions glutamic pyruvic transaminase activity in external blood and morphological picture of cardiac muscle, skeletal muscles, kidneys, cerebellum and lung tissue in guinea pigs were examined. Static homogeneous magnetic field as low as 0.005 T produced a statistically significant decrease in GPT activity. The animals were exposed to the magnetic field action for seven weeks 1 hour a day, 7 days a week. The range of observed changes of enzyme activity were determined by the duration of magnetic field. No morphological changes were observed.     

Vegetative reactions of dolphins to a change in the permanent magnetic field]

Vegetative responses of dolphin to changes in permanent magnetic field were studied. Electrocardiograms, cutaneogalvanic reactions, and respiration were registered. These parameters were compared with the functional behavior of dolphin: motion, sharp frequent expirations, and acoustic activity. Functional responses to permanent magnetic fields of 32; 108 and 168 microT were registered in 79.2, 63.3 and 52.9% of presentations, respectively. The frequency of responses in control at a power of geomagnetic field of 48.5 microT was 32.7%. Five latent periods were observed in the response of dolphin to changes in magnetic field (72% of responses). It is shown that dolphins have a high responsiveness to changes in permanent magnetic field "magnetic sense").     

Apparent biological effect of strong magnetic field on mosquito egg hatching

Apparent biological effects of strong magnetic fields were observed in the hatching behavior of fresh mosquito eggs in the center of 9.4 and 14.1 T magnets. In the first experiment performed at 20 +/- 1 degrees C, the hatching was delayed 32 h by a 9.4 T magnetic field and 71 h by a 14.1 T magnetic field. In the second experiment performed at 22 +/- 1 degrees C, the hatching was delayed 14 h by a 9.4 T magnetic field and 27 h by a 14.1 T magnetic field. In the magnetic field range of this study, the hatching delay increases nonlinearly with the intensity of the magnetic field. The experimental results also suggest that the biological effects of magnetic fields could be reversible or partially reversible to some extent.     

不同耕作措施对黄土高原旱地土壤呼吸的影响

在甘肃省定西市李家堡镇设置不同耕作措施试验,对春小麦、豌豆双序列轮作下的土壤呼吸及其对应时间的冠层温度、土壤水分进行了测定.结果表明：春小麦和豌豆在整个生育期内的土壤呼吸都表现出不同的变化趋势,春小麦地土壤呼吸在拔节前期、灌浆期和收后分别达到高峰期;豌豆地土壤呼吸在五叶期、吐丝期、开花结荚期和收后分别达到高峰期.免耕秸秆覆盖处理和传统耕作处理下春小麦地土壤呼吸日变化明显,免耕秸秆覆盖处理明显低于传统耕作处理;而豌豆地土壤呼吸日变化不明显.春小麦和豌豆冠层温度均与土壤呼吸呈显著线性相关,春小麦孕穗期相关性最高,灌浆期次之;豌豆开花结荚期相关性最高,分枝期次之.土壤含水量与土壤呼吸间存在极显著的凸型抛物线型相关,保护性耕作下的相关性都高于传统耕作,其中免耕秸秆覆盖处理的春小麦地和豌豆地土壤含水量与土壤呼吸的相关性在各层次土壤中均为最高,10～30 cm土壤含水量对春小麦地土壤呼吸的影响最大,5～10 cm土壤含水量对豌豆地土壤呼吸的影响最大.与传统耕作相比,免耕秸秆覆盖、免耕、传统耕作秸秆还田、传统耕作结合地膜覆盖、免耕结合地膜覆盖5种保护性耕作措施都能不同程度地降低土壤呼吸,其中免耕秸秆覆盖优势最明显.     

The influence of spatial pulsed magnetic field application on neuropathic pain after tibial nerve transection in rat

The purpose of the study was to examine the influence of the spatial variable magnetic field (induction: 150–300?µT, 80–150?µT, 20–80?µT; frequency 40?Hz) on neuropathic pain after tibial nerve transection. The experiments were carried out on 64 male Wistar C rats. The exposure of animals to magnetic field was performed 1?d/20?min., 5?d/week, for 28?d. Behavioural tests assessing the intensity of allodynia and sensitivity to mechanical and thermal stimuli were conducted 1?d prior to surgery and 3, 7, 14, 21 and 28?d after the surgery. The extent of autotomy was examined. Histological and immunohistochemical analysis was performed. The use of extremely low-frequency magnetic fields of minimal induction values (20–80?µT/40?Hz) decreased pain in rats after nerve transection. The nociceptive sensitivity of healthy rats was not changed following the exposition to the spatial magnetic field of the low frequency. The results of histological and immunohistochemical investigations confirm those findings. Our results indicate that extremely low-frequency magnetic field may be useful in the neuropathic pain therapy.     

强磁场重力环境对Pseudomonas aeruginosa N1207的影响

旨在研究由超导磁体产生的强磁场重力环境(HMGE)对Pseudomonas aeruginosa N1207的影响。该磁体是经特殊设计的大梯度超导磁体,可产生多个重力梯度(从失重到超重)以及相应的磁场强度,分别为(0 g,12 T)、(1 g,16 T)和(2 g,12 T)。Pseudomonas aeruginosa N1207在HMGE中分别诱变24h、48h和72h之后,从磁场强度最高(16 T)组别中筛选得到最佳的突变株M14808。结果表明M14808的鼠李糖脂产量提高了30%以上并且遗传特性稳定。比较生长周期发现,突变株到达指数生长期早于原始菌株。诱变实验结果表明强磁场是引起诱变的主要因素从而导致鼠李糖脂产量变化和生长周期改变。抗肿瘤实验结果显示双鼠李糖脂可抑制四株肿瘤细胞,分别为MCF-7、H460、HepG2和A549,双鼠李糖脂对MCF-7的效果最佳,其IC₅₀为125.13μg/ml。     

土壤紧实胁迫对黄瓜根系呼吸代谢的影响

用容重分别为1.20和1.55 g·cm^-3的土壤进行盆栽试验,研究了土壤紧实胁迫对‘津春4号’黄瓜根系呼吸代谢的影响.结果表明: 土壤紧实胁迫条件下,黄瓜根系中丙酮酸脱羧酶、乙醇脱氢酶和乳酸脱氢酶活性显著提高;无氧呼吸主要产物(乙醇、乙醛和乳酸)含量显著升高;参与有氧呼吸的苹果酸脱氢酶、琥珀酸脱氢酶和异柠檬酸脱氢酶活性显著下降,丙酮酸和琥珀酸含量显著提高,苹果酸含量显著下降.说明在土壤紧实胁迫条件下,黄瓜根系的有氧呼吸受到显著抑制,无氧呼吸过程加强.