共查询到20条相似文献,搜索用时 109 毫秒
1.
Bio-Rad公司的微量聚丙烯酰胺凝胶等电聚焦电泳(ModelⅢ Mini IEF Cell),不需要使用电极缓冲液,聚焦电泳时间仅用1.5小时,效果甚佳,但要使用该公司专有的凝胶支持薄膜,难于推广应用,我们经过试验,建立了一种简单、快速的微量凝胶等电聚焦电泳方法,40min完成等电聚焦过程,采用快速染色、脱色和干燥,将电泳图谱制成透明的薄膜,整个过程可以 相似文献
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我国不同地区东亚钳蝎(Buthus martensii Karsch)毒在三种类型电泳中的比较研究 总被引:1,自引:0,他引:1
用SDS—不连续聚丙烯酰胺凝胶板电泳、pH4.5—不连续聚丙烯酰胺凝胶板电泳和聚丙烯酰胺凝胶圆盘等电聚焦电泳,分离我国不同地区东亚钳蝎毒。其图谱表明:不同产地的东亚钳蝎毒蛋白的种类及含量存在一定的差异,地区相近,差异较小,反之亦然。 相似文献
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介绍了一种简易、高效、快速的生物同工酶分析方法——超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳方法。该方法与目前国内外市场销售的同种用途等电聚焦的产品 (如BIO- RAD公司的 model1 1 1 Mini IEF Cell)相比 ,具有以下优点 :实验操作灵活 ,用途更广 ;谱带清晰 ;每次可实验的样本量大 ;电泳所需时间短 ;实验费用低 ;适用于多种酶系统的同工酶和等位酶分析 ;凝胶干燥不需任何设备且能长期保存 ;样本用量少。 相似文献
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一种简便经济的薄层凝胶电泳保存方法——滤纸转移自干法 总被引:2,自引:0,他引:2
龚成新 《生物化学与生物物理进展》1990,17(4)
在分析性聚丙烯酰胺凝胶电泳特别是平板等电聚焦电泳中,薄层凝胶越来越被广泛采用,它有节省试剂、加样量少、电泳时间短、分辨率高及冷却效果好等优点。分析者都希望能将电泳染色后的凝胶理想地保存下来,目前所采用的方法均是直接将凝胶干燥在玻璃板或凝胶支持膜上(由于凝胶太薄不能转移),或者使用专用的透明保存膜,有时还要用真空干燥器干燥。 相似文献
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用SDS-不连续聚丙烯酰胺凝胶板电泳、pH4.5-不连续聚丙烯酰胺凝胶板电泳和聚丙烯酰胺凝胶圆盘等电聚焦电泳,分离我国不同地区东亚钳蝎毒。其图谱表明:不同产地的东亚钳蝎毒蛋白的种类及含量存在一定的差异,地区相近,差异较小,反之亦然。 相似文献
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醋酸纤维素膜上的蛋白质等电聚焦电泳 总被引:1,自引:1,他引:0
等电聚焦电泳一般以聚丙烯酰胺作为支撑物。但凝胶配制麻烦,两性载体用量多,价格昂贵。利用醋酸纤维素膜进行等电聚焦电泳,则可能克服上述缺点。但是醋酸纤维素膜的强电渗作用,影响了等电聚焦。过去解决这一问题的方法,条件复杂,要求高,有些试剂国内无法得到。我们试用了甲基化方法来封闭醋酸纤维素膜上的解离基团,有效地消除了电渗作用,不需转移而直接进行了大鼠血浆凝血酶原的酶联免疫染色。 相似文献
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用电内渗不同(0、0.03、0.08、0.20mr)的琼脂糖制成凝胶或电极缓冲液凝胶条,观察对电泳行为的影响.结果表明等电聚焦电泳必须使用无电内渗琼脂糖.不同电内渗的琼脂糖制成的电极缓冲液凝胶条对SDS电泳无显著影响,但对常规聚丙烯酰胺凝胶电泳有不同程度的影响,电内渗越高,越不利于电泳的进行. 相似文献
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超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳方法——介绍一种高效、快速的生物同工酶分析方法 总被引:17,自引:6,他引:11
介绍了一种简易、高效、快速的生物同工酶分析方法--超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳 方法。该方法与目前国内外市场销售的同种用途等电聚焦产品(如BIO-RAD公司的model11Mini IEF Cell)相比,具有以下优点:实验操作灵知,用途更广;谱带清晰;每次可实验的样本量大;电泳所需时间短;实验费用低;适用于多种酶系统的同工酶和等位酶分析;凝胶干燥不需任何设备且能长期保存;样本用量少。 相似文献
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J. C. Eilbeck 《Journal of mathematical biology》1983,16(3):233-249
Reaction diffusion equations are frequently used to model pattern formation problems in biology, but numerical experiments in two or three space dimensions can be expensive in computing time. We show that the spectral method with collocation is a particularly efficient method for the numerical study of the evolution of simple patterns in such models. In many cases of interest, the scheme is sufficiently simple and efficient for calculations to be carried out on a micro-computer. 相似文献
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PCR amplification of microdissected wheat chromosome arms in a simple 'single tube' reaction 总被引:11,自引:0,他引:11
Diego Albani Marie-José Côté Ken C. Armstrong Qianfa Chen Allan Segal Laurian S. Robert 《The Plant journal : for cell and molecular biology》1993,4(5):899-903
A novel method for the adaptor-mediated PCR amplification of microdissected chromosome arms is described. This simple and versatile protocol eliminates the need for enzymatic micromanipulation in nanoliter volumes and permits the efficient amplification of as little as two wheat chromosome arms in a 'single tube' reaction. 相似文献
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We consider two methods of estimating phenotype probabilities for a number of standard genetic markers like the ABO, MNSs, and PGM markers. The first method is based on the maximum likelihood estimates of the allele probabilities, and the second (multinomial) method uses the phenotype proportions in the sample. The latter is easy to use, the estimates are always unbiased, and simple formulae for variances are available. The former method, although giving more efficient estimates, requires the assumption of panmixia so that the Hardy-Weinberg law can be used. The two methods are compared theoretically, where possible, or by simulation. Under panmixia, the maximum likelihood estimates can be substantially more efficient than the multinomial estimates. The estimates are also compared in the codominant allele case for nonpanmictic populations. The question of efficiency is of importance when estimating the probability of obtaining a given set of phenotypes, i.e., the product of individual phenotype estimators. This problem is discussed briefly. 相似文献
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We develop a novel method of asserting the similarity between two biological sequences without the need for alignment. The proposed method uses free energy of nearest-neighbor interactions as a simple measure of dissimilarity. It is used to perform a search for similarities of a query sequence against three complex datasets. The sensitivity and selectivity are computed and evaluated and the performance of the proposed distance measure is compared. Real data analysis shows that is a very efficient, sensitive and high-selective algorithm in comparing large dataset of DNA sequences. 相似文献
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Rajiv Kumar Ranjan 《Analytical biochemistry》2010,402(1):91-92
Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers. 相似文献
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A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA. 总被引:1,自引:0,他引:1 下载免费PDF全文
A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, subcloning is unnecessary and a high efficiency of mutation (58-97%) was obtained. If two synthetic oligonucleotide primers are used, two separate mutations can be simultaneously created in a single reaction tube. 相似文献
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用RAPD技术鉴定两个小冰麦易位系 总被引:36,自引:1,他引:35
本文对从小冰麦异附加系中选育出的两个抗锈品系小冰麦33号,小冰麦34号及其原始亲本新曙光一号和抗源供体天兰冰草进行了RAPD分析,确定了小冰麦33号和小冰麦34号是含一段冰草DNA的易位系,文中还讨论了RAPD做为一种准确,快速鉴定易位系的方法的可行性。 相似文献
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A simple, rapid and efficient procedure is presented for the purification of human prostatic acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) to homogeneity. The method employs two steps suitable for use with large quantities of material, followed by chromatography on concanavalin A-Sepharose as its sole column step. The procedure also permits the recovery of purified enzyme in higher yields than earlier methods. 相似文献
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Not until recently has much attention been given to deriving maximum likelihood methods for estimating the intercept and slope parameters from a binormal ROC curve that assesses the accuracy of a continuous diagnostic test. We propose two new methods for estimating these parameters. The first method uses the profile likelihood and a simple algorithm to produce fully efficient estimates. The second method is based on a pseudo-maximum likelihood that can easily accommodate adjusting for covariates that could affect the accuracy of the continuous test. 相似文献
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A comparison was made of two methods for the extraction of enchytraeids, the O’Connor (1957) method and the Römbke (1995) method; the latter was originally proposed as a standard method for monitoring environmental pollution. The average number of enchytraeids extracted by the Römbke method was significantly greater. This simple and convenient method was shown to be as efficient as the traditional method when applied in both laboratory and field studies. 相似文献