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1.
Altered glutamatergic neurotransmission and neuronal metabolic dysfunction appear to be central to the pathophysiology of
Parkinson’s disease (PD). The substantia nigra pars compacta—the area where the primary pathological lesion is located—is
particularly exposed to oxidative stress and toxic and metabolic insults. A reduced capacity to cope with metabolic demands,
possibly related to impaired mitochondrial function, may render nigral neurons highly vulnerable to the effects of glutamate,
which acts as a neurotoxin in the presence of impaired cellular energy metabolism. In this way, glutamate may participate
in the pathogenesis of PD. Degeneration of dopamine nigral neurons is followed by striatal dopaminergic denervation, which
causes a cascade of functional modifications in the activity of basal ganglia nuclei. As an excitatory neurotransmitter, glutamate
plays a pivotal role in normal basal ganglia circuitry. With nigrostriatal dopaminergic depletion, the glutamatergic projections
from subthalamic nucleus to the basal ganglia output nuclei become overactive and there are regulatory changes in glutamate
receptors in these regions. There is also evidence of increased glutamatergic activity in the striatum. In animal models,
blockade of glutamate receptors ameliorates the motor manifestations of PD. Therefore, it appears that abnormal patterns of
glutamatergic neurotransmission are important in the symptoms of PD. The involvement of the glutamatergic system in the pathogenesis
and symptomatology of PD provides potential new targets for therapeutic intervention in this neuro-degenerative disorder. 相似文献
2.
3.
M Boyko D Stepensky BF Gruenbaum SE Gruenbaum I Melamed S Ohayon M Glazer Y Shapira A Zlotnik 《Neurochemical research》2012,37(10):2198-2205
Traumatic brain injury (TBI) and stroke lead to elevated levels of glutamate in the brain that negatively affect the neurological outcomes in both animals and humans. Intravenous administration of glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) enzymes can be used to lower the blood glutamate levels and to improve the neurological outcome following TBI and stroke. The objective of this study was to analyze the pharmacokinetics and to determine the glutamate-lowering effects of GOT and GPT enzymes in na?ve rats. We determined the time course of serum GOT, GPT, and glutamate levels following a single intravenous administration of two different doses of each one of the studied enzymes. Forty-six male rats were randomly assigned into one of 5 treatment groups: saline (control), human GOT at dose 0.03 and 0.06?mg/kg and porcine GPT at dose 0.6 and 1.2?mg/kg. Blood samples were collected at baseline, 5?min, and 2, 4, 8, 12, and 24?h after the drug injection and GOT, GPT and glutamate levels were determined. The pharmacokinetics of both GOT and GPT followed one-compartment model, and both enzymes exhibited substantial glutamate-lowering effects following intravenous administration. Analysis of the pharmacokinetic data indicated that both enzymes were distributed predominantly in the blood (central circulation) and did not permeate to the peripheral organs and tissues. Several-hour delay was present between the time course of the enzyme levels and the glutamate-lowering effects (leading to clock-wise hysteresis on concentration-effect curves), apparently due to the time that is required to affect the pool of serum glutamate. We conclude that the interaction between the systemically-administered enzymes (GOT and GPT) and the glutamate takes place in the central circulation. Thus, glutamate-lowering effects of GOT and GPT apparently lead to redistribution of the excess glutamate from the brain's extracellular fluid into the blood and can reduce secondary brain injury due to glutamate neurotoxicity. The outcomes of this study regarding the pharmacokinetic and pharmacodynamic properties of the GOT and GPT enzymes will be subsequently verified in clinical studies that can lead to design of effective neuroprotective treatment strategies in patients with traumatic brain diseases and stroke. 相似文献
4.
Ana María Estrada-Sánchez Teresa Montiel Lourdes Massieu 《Neurochemical research》2010,35(8):1156-1163
Excitotoxicity has been associated with the loss of medium spiny neurons (MSN) in Huntington’s disease (HD). We have previously
observed that the content of the glial glutamate transporters, glutamate transporter 1 (GLT-1) and glutamate-aspartate transporter
(GLAST), diminishes in R6/2 mice at 14 weeks of age but not at 10 weeks, and that this change correlates with an increased
vulnerability of striatal neurons to glutamate toxicity. We have also reported that inhibition of the glycolytic pathway decreases
glutamate uptake and enhances glutamate neurotoxicity in the rat brain. We now show that at 10-weeks of age, glutamate excitotoxicity
is precipitated in R6/2 mice, after the treatment with iodoacetate (IOA), an inhibitor of the glycolytic enzyme, glyceraldehyde-3-phosphate
dehydrogenase (GAPDH). IOA induces a larger inhibition of GAPDH in R6/2 mice, while it similarly reduces the levels of GLT-1
and GLAST in wild-type and transgenic animals. Results suggest that metabolic failure and altered glutamate uptake are involved
in the vulnerability of striatal neurons to glutamate excitotoxicity in HD. 相似文献
5.
Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder characterized by involuntary body movement,
cognitive impairment and psychiatric disturbance. A polyglutamine expansion in the amino-terminal region of the huntingtin
(htt) protein is the genetic cause of HD. Htt protein interacts with a wide variety of proteins, and htt mutation causes cell
signaling alterations in various neurotransmitter systems, including dopaminergic, glutamatergic, and cannabinoid systems,
as well as trophic factor systems. This review will overview recent findings concerning htt-promoted alterations in cell signaling
that involve different neurotransmitters and trophic factor systems, especially involving mGluR1/5, as glutamate plays a crucial
role in neuronal cell death. The neuronal cell death that takes place in the striatum and cortex of HD patients is the most
important factor underlying HD progression. Metabotropic glutamate receptors (mGluR1 and mGluR5) have a very controversial
role in neuronal cell death and it is not clear whether mGluR1/5 activation either protects or exacerbates neuronal death.
Thus, understanding how mutant htt protein affects glutamatergic receptor signaling will be essential to further establish
a role for glutamate receptors in HD and develop therapeutic strategies to treat HD. 相似文献
6.
Are the Tastes of Polycose and Monosodium Glutamate Unique? 总被引:2,自引:2,他引:0
To study whether Polycose and monosodium L-glutamate (L-MSG)have unique tastes differing from the traditional four basictastes, chemosensory profiles were established for Polycose,L-MSG and a group of related compounds (sucrose, maltose, monosodiumD-glutamate (D-MSG), sodium chloride, calcium chloride). Flavourswere assessed using whole-mouth tests in human subjects withnose open or clamped to reduce olfactory input. Polycose (amixture of glucose-based oligosaccharides) had a flavor consistingof an olfactory component and a maltose-like taste. L-MSG andD-MSG profiles differed from each other, and from NaCl and CaCl2.L-MSG had a lower threshold and a higher frequency of othertastes than the D form. The data do not support a polysaccharidetaste, but suggest a chiral receptor site for umamitaste. Chem. Senses 21: 341347, 1996. 相似文献
7.
The reported reduction of -ketoglutarate and ammonia by formate is much slower than described (Morowitz et al., 1995). The formate reduction if any is small under these conditions. Glutamate is produced from a reduction by a second molecule of -ketoglutarate involving an oxidative decarboxylation. 相似文献
8.
Tatiana Borisova Roman Sivko Arseniy Borysov Natalia Krisanova 《Cellular and molecular neurobiology》2010,30(7):1013-1023
The effect of the cholesterol-depleting agent methyl-β-cyclodextrin (MβCD) on exocytotic, transporter-mediated, tonic release,
the ambient level and uptake of l-[14C]glutamate was assessed in rat brain synaptosomes using different methodological approaches of MβCD application. The addition
of 15 mM MβCD to synaptosomes (the acute treatment, AT) immediately resulted in the extraction of cholesterol and in a two
times increase in the extracellular l-[14C]glutamate level. When 15 mM MβCD was applied to synaptosomes for 35 min followed by washing of the acceptor (the long-term
pretreatment, LP), this level was only one-third higher than in the control. The opposite effects of MβCD on tonic l-[14C]glutamate release and glutamate transporter reversal were found in AT and LP. Tonic release was dramatically enlarged in
AT, but decreased after LP. Transporter-mediated release was increased several times in AT, but attenuated in LP. Depolarization-evoked
exocytotic release of l-[14C]glutamate was completely lost in AT, whereas after LP, it was decreased by half in comparison with the control. Na+-dependent l-[14C]glutamate uptake was decreased by ~60% in AT, whereas in LP, it was lowered by ~40% only. The presence of MβCD in the incubation
media during AT caused dramatic dissipation of the proton gradient of synaptic vesicles that was shown with the pH-sensitive
dye acridine orange, whereas after LP, no statistically significant changes were registered in synaptic vesicle acidification.
It was concluded that the diverse changes in glutamate transport in AT and LP were associated with the difference in the functional
state of synaptic vesicles. 相似文献
9.
Jablonski MM Freeman NE Orr WE Templeton JP Lu L Williams RW Geisert EE 《Neurochemical research》2011,36(4):594-603
Müller cells serve many functions including the regulation of extracellular glutamate levels. The product of two genes, Slc1a3 [aka solute carrier family 1 (glial high affinity glutamate transporter), member 3] and Glul (aka glutamine synthetase) are the primary role players that transport glutamate into the Müller cell and convert it into
glutamine. In this study, we sought to identify the genetic regulation of both genes. Given their tightly coupled biological
functions, we predicted that they would be similarly regulated. Using an array of 75 recombinant inbred strains of mice, we
determined that Slc1a3 and Glul are differentially regulated by distinct chromosomal regions. Interestingly, despite their independent regulation, gene ontology
analysis of tightly correlated genes reveals that the enriched and statistically significant molecular function categories
of both directed acyclic graphs have substantial overlap, indicating that the shared functions of correlates of Slc1a3 and Glul include production and usage of ATP. 相似文献
10.
Burbaeva GSh Boksha IS Tereshkina EB Savushkina OK Starodubtseva LI Turishcheva MS 《Neurochemical research》2005,30(11):1443-1451
Amounts of glutamate metabolizing enzymes such as glutamate dehydrogenase (GDH), glutamine synthetase (GS), GS-like protein
(GSLP), and phosphate-activated glutaminase (PAG) were compared in prefrontal cortex of control subjects and patients with
Alzheimer disease (AD). The target proteins were quantified by ECL-Western immunoblotting in extracts from brain tissue prepared
by two different techniques separating enzymes preferentially associated with cytoplasm (GDH I and II isoenzymes, GS, and
partially GSLP) and membrane (GDH III, PAG, and partially GSLP) fractions. Amounts of all listed enzymes were found significantly
increased in the patient group compared with controls. Some links between the measured values were observed in the control,
but not in the AD patient group. The results may suggest for the pathological interruption of regulatory relations between
distinct enzymes of glutamate metabolism in brain of AD patients. 相似文献
11.
N. Hori C. R. Auker D. J. Braitman D. O. Carpenter 《Cellular and molecular neurobiology》1981,1(1):115-120
Aspartate and glutamate are the principal candidates for the excitatory neurotransmitter released by the lateral olfactory tract (LOT) in prepyriform cortex of the rat. Identity of action of the natural transmitter with exogenous glutamate and/or aspartate, however, has not yet been demonstrated. We show that bath-applied 2-amino-4-phosphonobutyric acid, a presumed specific glutamate antagonist, blocks LOT-stimulated prepyriform field potentials and single unit activity but not the single unit response to ionophoretically applied glutamate or aspartate in rat olfactory cortex slices. These results suggest that neither aspartate nor glutamate is the LOT transmitter. Responses to ionophoretically applied N-methyl-DL-aspartate, kainic acid, and DL-homocysteate were clearly decreased by 2-amino-4-phosphono-butyric acid. This suggests that these agents, usually presumed to be aspartate or glutamate agonists, act at different receptors than aspartate and glutamate.Supported by Armed Forces Radiobiology Research Institute, Defense Nuclear Agency, under Research Work Unit MJ 60203. The views presented in this paper are those of the authors. No endorsement by the Defense Nuclear Agency has been given or should be inferred. 相似文献
12.
Pereira Patrícia Elisabetsky Elaine Souza Diogo Onofre 《Neurochemical research》1997,22(12):1507-1510
Epilepsy is one of the most common neurological disorders. Even though antiepileptic drugs can afford a reasonably satisfactory treatment for 80% of diagnosed patients, chronic intractable epilepsy still affects a significant number of people and more effective and less harmful antiepileptic drugs are needed. Previous studies have shown that -decanolactone has dose-dependent sedative effects, including hypnotic, anticonvulsant and hypothermic properties in mice. The present study reports an inhibitory effect of -decanolactone on glutamate binding (96.8% with 5 mM) in rat cortex membranes. The non competitive nature of glutamate binding inhibition as a neurochemical correlate of the anticonvulsant activity of -decanolactone may be a relevant mode of action for further drug development. 相似文献
13.
Catania MV D'Antoni S Bonaccorso CM Aronica E Bear MF Nicoletti F 《Molecular neurobiology》2007,35(3):298-307
Group I metabotropic glutamate receptors (mGlu1 and mGlu5) are coupled to polyphosphoinositide hydrolysis and are involved
in activity-dependent forms of synaptic plasticity, both during development and in the adult life. Group I mGlu receptors
can also regulate proliferation, differentiation, and survival of neural stem/progenitor cells, which further support their
role in brain development. An exaggerated response to activation of mGlu5 receptors may underlie synaptic dysfunction in Fragile
X syndrome, the most common inherited form of mental retardation. In addition, group I mGlu receptors are overexpressed in
dysplastic neurons of focal cortical dysplasia and hemimegaloencephaly, which are disorders of cortical development associated
with chronic epilepsy. Drugs that block the activity of group I mGlu receptors (in particular, mGlu5 receptors) are potentially
helpful for the treatment of Fragile X syndrome and perhaps other neurodevelopmental disorders. 相似文献
14.
Huntington’s disease (HD) is caused by a CAG repeat expansion in the HD gene, but how this mutation causes neuronal dysfunction and degeneration is unclear. Inhibition of glutamate uptake, which
could cause excessive stimulation of glutamate receptors, has been found in animals carrying very long CAG repeats in the
HD gene. In seven HD patients with moderate CAG expansions (40–52), repeat expansion and HD grade at autopsy were strongly correlated
(r = 0.88, p = 0.0002). Uptake of [3H]glutamate was reduced by 43% in prefrontal cortex, but the level of synaptic (synaptophysin, AMPA receptors) and astrocytic
markers (GFAP, glutamate transporter EAAT1) were unchanged. Glutamate uptake correlated inversely with CAG repeat expansion
(r = −0.82, p = 0.015). The reducing agent dithiothreitol improved glutamate uptake in controls, but not in HD brains, suggesting irreversible
oxidation of glutamate transporters in HD. We conclude that impairment of glutamate uptake may contribute to neuronal dysfunction
and degeneration in HD.
Special issue article in honor of Dr. Frode Fonnum. 相似文献
15.
Fields RD 《Neuron glia biology》2010,6(4):209-211
Glutamate toxicity from hypoxia-ischaemia during the perinatal period causes white matter injury that can result in long-term motor and intellectual disability. Blocking ionotropic glutamate receptors (GluRs) has been shown to inhibit oligodendrocyte injury in vitro, but GluR antagonists have not yet proven helpful in clinical studies. The opposite approach of activating GluRs on developing oligodendrocytes shows promise in experimental studies on rodents as reported by Jartzie et al., in this issue. Group I metabotropic glutamate receptors (mGluRs) are expressed transiently on developing oligodendrocytes in humans during the perinatal period, and the blood-brain-barrier permeable agonist of group I mGluRs, 1-aminocyclopentane-trans-1,3-dicarboxylic acid (ACPD), reduces white matter damage significantly in a rat model of perinatal hypoxia-ischaemia. The results suggest drugs activating this class of GluRs could provide a new therapeutic approach for preventing cerebral palsy and other neurological consequences of diffuse white matter injury in premature infants. 相似文献
16.
Piyanard Boonnate Sakda Waraasawapati Wiphawi Hipkaeo Supattra Pethlert Amod Sharma Carlo Selmi Vitoon Prasongwattana Ubon Cha’on 《PloS one》2015,10(6)
Background
The amount of dietary monosodium glutamate (MSG) is increasing worldwide, in parallel with the epidemics of metabolic syndrome. Parenteral administration of MSG to rodents induces obesity, hyperglycemia, hyperlipidemia, insulin resistance, and type 2 diabetes. However, the impact of dietary MSG is still being debated. We investigated the morphological and functional effects of prolonged MSG consumption on rat glucose metabolism and on pancreatic islet histology.Methods
Eighty adult male Wistar rats were randomly subdivided into 4 groups, and test rats in each group were supplemented with MSG for a different duration (1, 3, 6, or 9 months, n=20 for each group). All rats were fed ad libitum with a standard rat chow and water. Ten test rats in each group were provided MSG 2 mg/g body weight/day in drinking water and the 10 remaining rats in each group served as non-MSG treated controls. Oral glucose tolerance tests (OGTT) were performed and serum insulin measured at 9 months. Animals were sacrificed at 1, 3, 6, or 9 months to examine the histopathology of pancreatic islets.Results
MSG-treated rats had significantly lower pancreatic β-cell mass at 1, 6 and 9 months of study. Islet hemorrhages increased with age in all groups and fibrosis was significantly more frequent in MSG-treated rats at 1 and 3 months. Serum insulin levels and glucose tolerance in MSG-treated and untreated rats were similar at all time points we investigated.Conclusion
Daily MSG dietary consumption was associated with reduced pancreatic β-cell mass and enhanced hemorrhages and fibrosis, but did not affect glucose homeostasis. We speculate that high dietary MSG intake may exert a negative effect on the pancreas and such effect might become functionally significant in the presence or susceptibility to diabetes or NaCl; future experiments will take these crucial cofactors into account. 相似文献17.
K. Takatsuki S. Kawahara S. Kotani H. Mori M. Mishina Y. Kirino 《Journal of biological physics》2002,28(3):539-547
Cerebellar long-term depression (LTD) at the parallel fiber-Purkinje cell synapses has been proposed to be a neural substrate for classical eyeblink conditioning. Mutant mice lacking the glutamate receptor subunit 2 (GluR2), in which the cerebellar LTD is disrupted, exhibited a severe impairment in the delay eyeblink conditioning with a temporal overlap of CS and US. However, they learned normally trace and delay conditioning without CS-US overlap, suggesting a learning mechanism which does not require the cerebellar LTD.In the present study, we tested possible involvement of the hippocampus in this cerebellar LTD-independent learning. We examined effects of scopolamine and hippocampal lesion on the delay conditioning without CS-US overlap. TheGluR2 mutant mice that received scopolamine or aspiration of the dorsalhippocampus together with its overlying cortex exhibited a severe impairment in learning, while the control mutant mice that received saline or aspiration of the overlying cortex learned normally. In contrast, wild-type mice that received either treatment learned as normally as the control wild-type mice. These results suggest that the hippocampus is essential in the cerebellar LTD-independent learning in the GluR2 mutant mice, indicating a newrole of hippocampus in the paradigm with a short trace interval. 相似文献
18.
Wei-Lun Hsu ) Hui-Wen Chung ) Chih-Yueh Wu ) Huei-Ing Wu ) Yu-Tao Lee ) En-Chan Chen ) Weilun Fang ) Yen-Chung Chang ) 《The Journal of biological chemistry》2015,290(34):20748-20760
Glutamate is the principal excitatory neurotransmitter in the mammalian CNS. By analyzing the metabolic incorporation of azidohomoalanine, a methionine analogue, in newly synthesized proteins, we find that glutamate treatments up-regulate protein translation not only in intact rat cortical neurons in culture but also in the axons emitting from cortical neurons before making synapses with target cells. The process by which glutamate stimulates local translation in axons begins with the binding of glutamate to the ionotropic AMPA receptors and metabotropic glutamate receptor 1 and members of group 2 metabotropic glutamate receptors on the plasma membrane. Subsequently, the activated mammalian target of rapamycin (mTOR) signaling pathway and the rise in Ca2+, resulting from Ca2+ influxes through calcium-permeable AMPA receptors, voltage-gated Ca2+ channels, and transient receptor potential canonical channels, in axons stimulate the local translation machinery. For comparison, the enhancement effects of brain-derived neurotrophic factor (BDNF) on the local protein synthesis in cortical axons were also studied. The results indicate that Ca2+ influxes via transient receptor potential canonical channels and activated the mTOR pathway in axons also mediate BDNF stimulation to local protein synthesis. However, glutamate- and BDNF-induced enhancements of translation in axons exhibit different kinetics. Moreover, Ca2+ and mTOR signaling appear to play roles carrying different weights, respectively, in transducing glutamate- and BDNF-induced enhancements of axonal translation. Thus, our results indicate that exposure to transient increases of glutamate and more lasting increases of BDNF would stimulate local protein synthesis in migrating axons en route to their targets in the developing brain. 相似文献
19.
Dopamine and glutamate systems are both involved in cognitive, behavioral, and motor processes. Dysfunction of dopamine–glutamate interplay has been suggested in several psychotic diseases, above all in schizophrenia, for which there exists a need for novel medications. Intracellular calcium-dependent transduction pathways are key determinants of dopamine–glutamate interactions, which take place mainly, albeit not exclusively, in the postsynaptic density (PSD), a highly specialized postsynaptic ultrastructure. Stimulation of dopamine and glutamate receptors modulates the gene expression and the function of specific PSD proteins, the “scaffolding” proteins (Homer, Shank, and PSD95), belonging to a complex Ca2+-regulated network that integrates and converges dopamine and glutamate signaling to appropriate nuclear targets. Dysfunction of scaffolding proteins leads to severe impairment of Ca2+-dependent signaling, which may underlie the dopamine–glutamate aberrations putatively implicated in the pathogenesis of psychotic disorders. Antipsychotic therapy has been demonstrated to directly and indirectly affect the neuronal Ca2+-dependent pathways through the modulation of PSD scaffolding proteins, such as Homer, therefore influencing both dopaminergic and glutamatergic functions and enforcing Ca2+-mediated long-term synaptic changes. In this review, we will discuss the role of PSD scaffolding proteins in routing Ca2+-dependent signals to the nucleus. In particular, we will address the implication of PSD scaffolding proteins in the intracellular connections between dopamine and glutamate pathways, which involve both Ca2+-dependent and Ca2+-independent mechanisms. Finally, we will discuss how new strategies for the treatment of psychosis aim at developing antipsychotics that may impact both glutamate and dopamine signaling, and what should be the possible role of PSD scaffolding proteins. 相似文献
20.
Synaptic vesicular accumulation of glutamate is a vital initial step in glutamate transmission. We have previously shown that Rose Bengal, a polyhalogenated fluorescein analog, is a potent inhibitor of glutamate uptake into synaptic vesicles. Here, we report the structural features of Rose Bengal required for this inhibition. Various Rose Bengal-related compounds, with systematic structural variations, were tested. Results indicate that the four iodo groups and the phenyl group attached to the xanthene moiety are critical for potent inhibitory activity. Replacement of these groups with two iodo groups and an alkyl group, respectively, results in substantial reduction in potency. Of further interest in creating high potency is the critical nature of the oxygen atom which links the two benzene rings of xanthene. Thus, the phenyl group and multiple iodo groups, as well as the bridging oxygen of xanthene, are crucial elements of Rose Bengal required for its potent inhibitory action. 相似文献